-
No products found
because this supplier's products are not listed.
Diego López-Márquez, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 150 µM 3′,5′-Dimethoxy-4′-hydroxyacetophenone (acetosyringone; SIGMA, USA) at a 23.7×108 cfu/ml (OD600=0.75 ...
-
No products found
because this supplier's products are not listed.
Francis Ledesma, et al.,
bioRxiv - Bioengineering 2023
Quote:
... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.
-
No products found
because this supplier's products are not listed.
Ashwin Narayanan, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... concentrations was assessed using reduction in WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]- 1,3-benzene Disulfonate) to water-soluble formazan (Roche, France). Cells were seeded in 96-well plates at 2 × 104 cells/well and treated with the different NAM concentrations at 37°C ...
-
No products found
because this supplier's products are not listed.
Ludovica Iovino, et al.,
bioRxiv - Neuroscience 2022
Quote:
... were incubated for 10 min at 37 °C in the presence of 10 µM 2-amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile (UCPH-101; Abcam 120309, UK), a specific excitatory amino acid transporter 1 (EAAT1/Glast ...
-
No products found
because this supplier's products are not listed.
Fushun Wang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 5 µM, Tocris); Tetrodotoxin (TTX ...
-
No products found
because this supplier's products are not listed.
Liang Wang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... #4: 5’-CCGGTTTAGCTGAAGATTCAA-3’ (SI00443779, Qiagen), GTPBP10 siRNA 5’-TTGCGTGTTGTTCAGAAAGTA-3’ (SI04308647 ...
-
No products found
because this supplier's products are not listed.
Nitin Wadhwani, et al.,
bioRxiv - Cancer Biology 2022
Quote:
SJ-GBM2 and SF8628 cells were used to determine if reducing WDR82 through inducible knockdown affected cell viability 1×104 cells/100μl were plated in 96-well plates with complete cell culture medium with or without Dox (2μg/ml), and subjected to 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega) assay.
-
No products found
because this supplier's products are not listed.
Ana Monfort-Vengut, et al.,
bioRxiv - Cancer Biology 2024
Quote:
WNK1 depletion by CRISPR-Cas9 editing was done by using two independent gRNAs targeting Exon-1 (5’-CGCCGACGCTGTGACCGGC-3’) and Exon-4 (5’-ACTTACACTGGTCACGCGA-3’) cloned into pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W backbone vector (Addgene #67974). TET-ON-Cas9 expressing cells were infected and BFP-positive cell FACS sorted ...
-
No products found
because this supplier's products are not listed.
Nicole B. Webster, Néva P. Meyer,
bioRxiv - Developmental Biology 2023
Quote:
... and 1:400 rabbit anti-phosphorylated-SMAD1/5/8 (pSMAD1/5/8; clone 41D10, Cell Signaling Technologies). Secondary antibodies used were as follows ...
-
No products found
because this supplier's products are not listed.
John Fehilly, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... anti-pan-14-3-3 (B-8) (Santa Cruz Biotechnology, sc-133233, 1:1000), anti-14-3-3η (D23B7 ...
-
No products found
because this supplier's products are not listed.
Benjamin C. Shaw, et al.,
bioRxiv - Molecular Biology 2022
Quote:
The cDNA samples from the anterior cingulate samples were amplified using primers corresponding to TREM2 exon 1 (5’-CCTGACATGCCTGATCCTCT-3’) and exon 5 (5’-GTGTTCTTACCACCTCCCC-3’) with Q5 high-fidelity hot-start polymerase (NEB # M0493L). Thermocylcing parameters were as follows ...
-
No products found
because this supplier's products are not listed.
Justin E. Silpe, et al.,
bioRxiv - Microbiology 2021
Quote:
... 40 μg mL−1 5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactosidase (X-gal, Takara Bio), and 500 μM isopropyl beta-D-1-thiogalactopyranoside (IPTG ...
-
No products found
because this supplier's products are not listed.
Kevin C. Courtney, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′,5′- bisphosphate) (PI(4, 5)P2) were obtained from Avanti Polar Lipids. HEPES was from Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Kaitlin Morse, Sarah Swerdlow, Elçin Ünal,
bioRxiv - Molecular Biology 2023
Quote:
4–8 μl of samples and 3 μl of Precision Plus Protein Dual Color Standard (1610374, Bio-Rad) were loaded into 4–12% Bis-Tris Bolt gels (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Martijn AJ Koppens, et al.,
bioRxiv - Cell Biology 2021
Quote:
... pSmad1/5/8 (Merck, AB3848-I, 1/200), Ki67 (Cell Signaling Technology ...
-
No products found
because this supplier's products are not listed.
Merve Gozel, et al.,
bioRxiv - Immunology 2024
Quote:
... as positive markers for MSCs and CD3/4/8 (CD4/8/3, BD Tritest™; BD Biosciences San Jose, CA) CD56 (NCAM16.2 ...
-
No products found
because this supplier's products are not listed.
Andrew L. Eagle, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Male C57Bl/6J mice (3–5/cage, 7–8-week old upon arrival from Jackson Labs) were allowed at least 5 d to acclimate to the facility prior to any experimental procedures ...
-
No products found
because this supplier's products are not listed.
Verena Schmied, et al.,
bioRxiv - Neuroscience 2024
Quote:
... 4-5 3D-organoids were placed into one well of an 8-well chamber (IBIDI, #80826) and covered with 3% (w/v ...
-
No products found
because this supplier's products are not listed.
Teresa Neuwirth, et al.,
bioRxiv - Immunology 2024
Quote:
... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
-
No products found
because this supplier's products are not listed.
Fang Xu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 8% (w/v) paraformaldehyde (PFA; 4% final concentration; 157-8, Electron Microscopy Sciences), distilled water ...
-
No products found
because this supplier's products are not listed.
Christophe Royer, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... 3 to 4 week old CD-1 females (Charles River UK) were injected intraperitoneally with 5 IU of PMSG (Intervet ...
-
No products found
because this supplier's products are not listed.
Elvin Wagenblast, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Between 1×10^3 – 5×10^4 cells were re-suspended in 20μl Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the CRISPR/Cas9 gRNA RNP complex ...
-
No products found
because this supplier's products are not listed.
Gina Partipilo, et al.,
bioRxiv - Bioengineering 2024
Quote:
... disodium;4-[3-pyridin-2-yl-6-(4- sulfonatophenyl)-1,2,4-triazin-5-yl]benzenesulfonate hydrate (Ferrozine, VWR) All media components were autoclaved or sterilized using 0.2 μm PES filters.
-
No products found
because this supplier's products are not listed.
Timothy J. Mottram, et al.,
bioRxiv - Microbiology 2023
Quote:
... 4 μl of the 3’-5’-adapter-ligated RNA was mixed with barcoded RT primers (Illumina) and cDNA synthesis was performed using SuperScript™ II Reverse Transcriptase (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Saijuan Xu, et al.,
bioRxiv - Bioengineering 2024
Quote:
... and 5 ng ml−1 human IL-3 (PeproTech, 200-03) as EDM-1 ...
-
No products found
because this supplier's products are not listed.
Anthony Gurunian, Keren Lasker, Ashok A. Deniz,
bioRxiv - Biophysics 2024
Quote:
... 4-[2-[6-(dioctylamino)-2-naphthalenyl]ethenyl]-1-(3-sulfopropyl)-pyridinium (di-8-anepps) was purchased from Cayman Chemical (Ann Arbor, Michigan). Mineral Oil (USP grade ...
-
No products found
because this supplier's products are not listed.
Jinhyung Lee, et al.,
bioRxiv - Bioengineering 2024
Quote:
Female 6- to 8-week-old NU/J mice were inoculated subcutaneously with 5 × 106 SKOV-3 cells supplemented with Matrigel (Corning, #354234), and allowed to grow for 4 weeks until the tumor size was 200 mm3 ...
-
No products found
because this supplier's products are not listed.
Annika Ullrich, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
-
No products found
because this supplier's products are not listed.
Flora D’anna, et al.,
bioRxiv - Genomics 2020
Quote:
... 3 times 5 min washes with TNT and signal amplification for 8 min using Cy3 (NEL704A001KT, Perkin Elmer) 1:50 in amplification diluent ...
-
No products found
because this supplier's products are not listed.
Peng Liu, Ian P. Lapcinski, Karen H. Ashe,
bioRxiv - Biochemistry 2024
Quote:
... that recognizes amino acids 3-8 of Aβ and the Protein G Sepharose 4 Fast Flow resin (GE healthcare) (Tables 2 and 3 ...
-
No products found
because this supplier's products are not listed.
Matthew J. Burke, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... a J2-4 reverse primer 5’-(GGATAGATCTCTGAGGAGACGGTGACCAGGAG-3’) and HERC II polymerase (Agilent), following the manufacturer’s recommended reaction conditions ...
-
No products found
because this supplier's products are not listed.
Anika Schumacher, et al.,
bioRxiv - Bioengineering 2023
Quote:
... 8 μM GSK-3 inhibitor CHIR99021 (R&D Systems), 200 ng/ml fibroblast growth factor 9 (FGF9 ...
-
No products found
because this supplier's products are not listed.
Mengmeng Jin, et al.,
bioRxiv - Neuroscience 2024
Quote:
... in a feeder free fashion and passaged every 4∼5 days at a 1:4∼1:6 ratio using ReLeSR (Stemcell technologies) as described previously 88,95 ...
-
No products found
because this supplier's products are not listed.
N. Sumru Bayin, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Image stacks were obtained every 3-4 minutes for 6-8 hours using a LSM880 (Leica) with an environmental chamber (37°C with 5% CO2) ...
-
No products found
because this supplier's products are not listed.
Han Zhu, et al.,
bioRxiv - Cell Biology 2022
Quote:
Samples were incubated on a rotator for 5 min at 4°C and then centrifuged at 500g for 5 min (Eppendorf, 5920R; 4°C, ramp speed of 3/3). Supernatant was removed and pellet was resuspended in sort buffer [1mM EDTA (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Buse Baran, et al.,
bioRxiv - Plant Biology 2024
Quote:
... Guide RNA sequences “5-CGUGUCACGACGGAGUGGAU-3” and “5-AUGUUGUAUGUGCCUAUACG-3” were synthesized by GenScript by adding the Cas12a scaffold sequence (5-UAAUUUCUACUCUUGUAGAU-3 ...
-
No products found
because this supplier's products are not listed.
Lisa M. Hom, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Caspase 8 (Proteintech; 13423-1-AP), Caspase 1 (Proteintech ...
-
No products found
because this supplier's products are not listed.
Yun Deng, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... with 5-bromo-4-chloro-3-indolyl-phosphate nitro blue tetrazolium staining (Vector Laboratories, Burlingame, CA, USA). 10 ∼ 30 embryos were used for each probe ...
-
No products found
because this supplier's products are not listed.
Emily N. P. Prowse, et al.,
bioRxiv - Cell Biology 2024
Quote:
... We loaded 2 mL of the homogenate mixture on top of a 3.92 ml 62% sucrose in MAB cushion in an Ultra-Clear 5/8” X 4” Beckman Coulter tube (344061, Beckman Coulter). We added the remaining sucrose in MAB solutions ...
-
No products found
because this supplier's products are not listed.
Sophie Dürauer, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and cell numbers were recorded every 8 h for 3 days using Cytation 5 (BioTek) equipped with a 4x objective and the Gen5 software (ver ...
-
No products found
because this supplier's products are not listed.
Coralie Hérent, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Cy-3 or Cy-5 (1:500, Jackson ImmunoResearch). Sections were counterstained with a fluorescent Nissl stain (NeuroTrace 435/445 blue ...
-
No products found
because this supplier's products are not listed.
Marc Duque, et al.,
bioRxiv - Neuroscience 2021
Quote:
... performing half media changes every 3-4 days with fresh NbActiv1 supplemented with PrimocinTM (InvivoGen ant-pm-1). Neurons infected with GCaMP6f as stated above were infected with AAV9-hSyn-Cre (Addgene #105553-AAV9 ...
-
No products found
because this supplier's products are not listed.
Kirtan Joshi, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... A volume of 150 µL of AdCre (1×1011 PFU/mL) ± adjuvants porcine IL-8 (5 ng/mL) (NBP2-35234; Novus Biological) and ± adjuvants Polybrene (1:100 ...
-
No products found
because this supplier's products are not listed.
Sara Lago, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... pRPA32 1:1000 (Bethyl, A300-246A-8) and 53BP1 1:100 (Millipore ...
-
No products found
because this supplier's products are not listed.
Zuzanna M. Luczak-Sobotkowska, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Cryosections were first blocked in PBS containing 10% donkey serum in a 0.1% Triton X-100 solution for 2 hours and then incubated in a 3% donkey serum buffer overnight at 4 °C with guinea pig anti-Tmem119 antibody (1:1,000, Synaptic System). After that ...
-
No products found
because this supplier's products are not listed.
Valentina Gandin, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... using the following two primer sequences (T7 Forward: 5’-TAATACGACTCACTATAGCGTCATC-3’; Reverse: 5’-TTGTCGCACGTTCGGTGTCG-3’) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research, 11-302). dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB ...
-
No products found
because this supplier's products are not listed.
Wenjian Lv, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were passaged (1:12) every 3-4 days using StemMACS Passaging Solution XF (Miltenyi Biotec) and Y-27632 dihydrochloride (Santa Cruz ...
-
No products found
because this supplier's products are not listed.
He-Chin Hsieh, et al.,
bioRxiv - Microbiology 2023
Quote:
... and BA.4/5 (Genetex, Cat. No. GTX137098-pro) were mixed with RBD-LTA ...
-
No products found
because this supplier's products are not listed.
Nina R. Montoya, et al.,
bioRxiv - Microbiology 2022
Quote:
... The plates were developed with TMB (3, 3’, 5, 5’ - Tetramethylbenzidine) ELISA Peroxidase Substrate (Rockland Immunochemical, Limerick, PA), and absorbance was measured on a plate reader at 655 nm.
-
No products found
because this supplier's products are not listed.
Annelot C. M. van Esbroeck, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... After rinsing the membrane with TBS-T (3 × 5 min) and TBS (3 × 5 min) fluorescence was detected by scanning on the Odyssey CLx (LI-COR Biosciences).