Labshake search
Citations for GenScript :
1 - 33 of 33 citations for Yeast Extracts since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: The yeast Rev1 construct was purchased from GenScript. All Rev1 proteins were expressed in BL21(DE3 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... coli or yeast and synthesized by GenScript (Piscataway, NJ) or Twist Biosciences (San Francisco ...
-
bioRxiv - Synthetic Biology 2021Quote: ... ΔVP1 was codon-optimised for yeast and synthesised by GenScript. All other synthetic genes were manually codon-optimised for S ...
-
bioRxiv - Genetics 2021Quote: ... we stained the yeast with recombinant CoV2 RBD-His6 (Genscript) at diminishing concentrations through the rounds (5nM ...
-
bioRxiv - Immunology 2022Quote: ... with probabilities matching yeast codon usage (GenScript Codon Usage Frequency Table). The DNA-encoded peptide sequences were flanked by invariant sequences from the yeast construct for handles in amplification and cloning ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The yeast THI4 coding sequence (ScTHI4) was synthesized by GenScript (Piscataway, NJ), and PCR amplified using primers 1 and 2 ...
-
bioRxiv - Biochemistry 2021Quote: The construct for the yeast Rev1 catalytic core was purchased from GenScript. All Rev1 constructs were transformed and expressed in BL21(DE3 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Total protein extracts were separated by SDS-PAGE (Genscript, USA) and then blotted to NC membranes (Millipore ...
-
bioRxiv - Plant Biology 2024Quote: ... total protein extracts were loaded onto commercial precast gels (SurePage; GenScript) and immunoblotted with anti-GFP antibodies (Abcam Ab290 ...
-
bioRxiv - Microbiology 2019Quote: ... discoideum crude extract with an anti-FLAG rabbit polyclonal antibody (GenScript, USA). For ectopic expression assays ...
-
bioRxiv - Microbiology 2023Quote: ... Protein extracts were resolved in an ExpressPlus 4-12% gradient gel (GenScript), electroblotted to a nitrocellulose membrane ...
-
bioRxiv - Molecular Biology 2020Quote: ... The extracts were fractionated on a 4-20% ExpressPlus™ PAGE gel (Genscript) using SDS-MOPS buffer and transferred onto nitrocellulose membrane (BioTrace™ NT Nitrocellulose transfer membrane ...
-
bioRxiv - Biochemistry 2021Quote: ... Full-length DHFR was expressed in yeast from pTR1412 lacking the URA3-HA-GFP fusion (Genscript). Point mutations were generated by Genscript ...
-
bioRxiv - Molecular Biology 2023Quote: The nuclear extract was subsequently incubated with MonoRab™ Anti-DYKDDDDK affinity resin (Genscript, L00766) for 2 hours and then washed with BC300 buffer (20 mM HEPES at pH 7.9 ...
-
bioRxiv - Biochemistry 2020Quote: ... Full-length codon-optimized yeast HSP104 fused C-terminally to an HA-tag was expressed from pcDNA3.1 (Genscript). Human codon-optimized HSPH1 fused C-terminally to a Myc-tag was expressed from pcDNA3.1 (Genscript) ...
-
bioRxiv - Cell Biology 2020Quote: ... the DNA encoding for mScarlet was codon optimized for expression in yeast and chemically synthesized (GenScript, Piscataway, NJ). mScarlet was then PCR amplified and cloned as a PstI/BamHI fragment into the pCM189 plasmid ...
-
bioRxiv - Cell Biology 2024Quote: The Complementary Determining Sequence (CDS) encoding PfMFS_DT was codon-optimized for expression in yeast (GenScript Biotech, United States) (Supplementary Figure S2 ...
-
bioRxiv - Genetics 2022Quote: The pancreatic isoform of human GCK (Ensembl ENST00000403799.8) was codon optimized for yeast expression and cloned into pDONR221 (Genscript). The initial test set GCK variants were generated by Genscript ...
-
bioRxiv - Genetics 2022Quote: ... Human GKRP (Ensembl ENST00000264717.7) was codon optimized for yeast expression and cloned into pDONR221 with an N-terminal HA-tag (Genscript). For yeast expression ...
-
bioRxiv - Biochemistry 2023Quote: The DNA sequence of pancreatic human GCK (Ensembl ENST00000403799.8) was codon optimized for yeast and cloned into pDONR221 (Genscript). Selected missense variants were generated by Genscript ...
-
bioRxiv - Biochemistry 2021Quote: ... gene was codon-optimized and cloned into the p423_GAL1 yeast expression vector as an N-terminal Flag (DYKDDDDK) and C-terminal decahistidine (10X His) tagged fusion protein (GenScript) (Fig ...
-
bioRxiv - Bioengineering 2023Quote: ... BsmbI and NotI sites for bccyb5 (UniProt identifier A0A384K1M2) and bccbr1 (UniProt identifier A0A384JNH0) were codon-optimised for yeast and provided by Genscript Biotech Corp ...
-
bioRxiv - Biochemistry 2023Quote: ... the gene was inserted between the BamHI and EcoRIsites of the vector pGEX-4T-1 with an N-terminal GST tag followed by an HRV 3C protease cleavage site. The DNA sequence encoding yeast (S. cerevisiae) Ubc4 was synthesized by GenScript and its codon was optimized for E ...
-
bioRxiv - Molecular Biology 2023Quote: ... The pBY011 plasmid encoding yeast CHD1 gene under the GAL1/10 promoter was obtained from a DNASU plasmid repository and altered by GenScript, where FLAG tag and NES-NES sequences were added to the N- and C-termini of the gene ...
-
bioRxiv - Biochemistry 2024Quote: ... gene was codon-optimized and cloned into the p423_GAL1 yeast expression vector as an N-terminal Flag (DYKDDDDK) and C-terminal deca-histidine (10X His) tagged fusion protein (GenScript) (Supplementary Fig ...
-
bioRxiv - Biochemistry 2019Quote: ... xlAZIN2 and xlODC1 were purified from the cell extracts by affinity chromatography using an anti-Flag resin (GenScript) in accordance with the instructions of the supplier ...
-
bioRxiv - Microbiology 2020Quote: ... regions of the TSPs Sh-TSP2 and MS3_01370 (predicted using TMPRED) were codon optimized based on yeast codon usage preference and synthesized by GenScript (NJ, US). The synthesized coding DNAs with a 6-His-tag expressed at the C-terminus were cloned into the pPinkα-HC expression vector (Thermofisher ...
-
bioRxiv - Synthetic Biology 2022Quote: ... coli strain DH10B/TOP10 was as previously described.14,15 Bacterial THI4s were codon-optimized for yeast and synthesized by GenScript (Piscataway, NJ); the recoded sequences are given in Table S1 ...
-
bioRxiv - Biochemistry 2023Quote: The coding sequence of the 11 subunits of mouse CMG were codon optimised for high-level expression in budding yeast cells and produced by DNA synthesis (GenScript Biotech). A previously described ‘yeast toolkit’ (Lee et al. ...
-
bioRxiv - Biochemistry 2023Quote: ... or with p416-GPD-PfNF54_070015200 (hereafter referred to as p416-PfPolK, i.e., cloned with the Plasmodium PfPolK gene optimized by Genscript for expression in yeast). Yeast expression vectors were transformed into yeasts by the lithium acetate method (Gietz & Woods ...
-
bioRxiv - Biochemistry 2023Quote: ... genes flanked by BamHI and HindIII sites were codon-optimized for expression in budding yeast (Figures S1A and S1B) (GenScript; Piscataway, NJ). Codon-optimized γ1-actin (ACTG1 ...
-
bioRxiv - Immunology 2021Quote: ... and RBD219-N1C1 (residues 332-549, C538A) were codon-optimized based on yeast codon usage preference and synthesized by GenScript (Piscataway, NJ, USA), followed by subcloning into the Pichia secretory expression vector pPICZαA (Invitrogen ...
-
bioRxiv - Cell Biology 2019Quote: ... The polyclonal antibody used to purify γ-TuRC from Xenopus egg extract was generated against a purified γ-tubulin peptide (amino acids 412-451) through a commercial vendor (Genscript). The presence of γ-TuRC during its purification was tracked via Western blotting using the GTU88 (Sigma-Aldrich ...