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Citations for GenScript :
1 - 50 of 187 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Plasmid construction and validation were performed by GenScript (Genscript Biotech Corp ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid constructions were conducted by GenScript (Piscataway, NJ, USA). The plasmids pGFP-P2A-CFTR-WT-T7 and pGFP-P2A-CFTR-Δex23-T7 encode recombinant GFP-P2A-CFTR proteins ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All plasmids and strain construction were confirmed by Sanger sequencing (Genscript).
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bioRxiv - Microbiology 2023Quote: ... For construction of pNW2315 the required fragment was synthesised by GenScript and inserted into the pCC1 vector ...
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bioRxiv - Microbiology 2023Quote: ... The two sets of oligo libraries were synthesized on two separate chips by GenScript. The mutant sequences of each frame were amplified with Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Two TAP1 gRNAs (Genscript, USA) were introduced by lentiviral transduction ...
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bioRxiv - Neuroscience 2020Quote: ... Purified monomer protein passed through two to three rounds of endotoxin removal (Endotoxin removal kit, GenScript) to reach a level of <0.1 EU mg-1 ...
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bioRxiv - Neuroscience 2023Quote: ... Purified monomer protein was subjected to two to three rounds of endotoxin removal (Endotoxin removal kit, GenScript) until a level of <0.1 EU per mg was achieved ...
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bioRxiv - Cell Biology 2021Quote: ... two peptides were synthesised by GenScript. One peptide contained a phosphorylated serine (P) ...
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bioRxiv - Cancer Biology 2022Quote: ... a hybrid probe library with 6394 probes was designed and synthesized by GenScript. Magnetic bead-based capturing ...
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bioRxiv - Bioengineering 2022Quote: ... Two GenPart fragments were synthesized from GenScript for each plasmid (OA-1053A or OA-1053B) ...
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bioRxiv - Synthetic Biology 2022Quote: ... These two cassettes were synthesized by Genscript Inc ...
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bioRxiv - Cell Biology 2023Quote: DNA sequences used for plasmid construction were either amplified from the cDNA of HEK293T cells or purchased from GenScript and WZ Biosciences ...
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bioRxiv - Cancer Biology 2019Quote: Two vectors were prepared by GenScript (Piscataway, NJ) with puromycin resistance genes and the flanking guide RNA (gRNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Two Mpro gene constructs were ordered from Genscript, USA in a pET28a(+ ...
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bioRxiv - Microbiology 2020Quote: ... Two additional fragments were de novo synthesized (Genscript) and amplified by PCR (primers are listed in Table S6) ...
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bioRxiv - Biochemistry 2021Quote: ... A further two constructs were synthesised by GenScript as above but with point mutations leucine to alanine (in bold and underlined ...
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bioRxiv - Immunology 2021Quote: ... Two gBlock gene fragments were synthesized by Genscript to encode the modified TCRα chain ...
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bioRxiv - Bioengineering 2019Quote: ... a DNA block composed of the leader sequence of the chromosomal CRISPR2 as a promoter and two CRISPR repeats spaced by two BsaI restriction sequences in opposite orientation was synthesized from GenScript (Nanjing, China), and used as a template for PCR amplification with the primer pair of L2R-XbaI-F/L2R-EcoRI-R (Table 3) ...
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bioRxiv - Immunology 2021Quote: ... based on antibody-mediated blockage of pseudovirus infection of cells containing SARS-CoV-2 receptors was performed on serum specimens using two commercially available SARS-CoV-2 LVV-PsN Kit (SC2087A; SC2087L; GenScript, Nanjing, China) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Two plasmids (pMT-1 and pMT-2) were constructed by Genscript® using vector pUCP22.
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bioRxiv - Immunology 2021Quote: ... the VH and VL regions of 2H1 and 2H1-hybrid antibodies were codon-optimized using a proprietary algorithm and synthetized by GenScript and then cloned into the commercially available pFUSE vectors (InvivoGen) ...
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bioRxiv - Plant Biology 2022Quote: ... a DNA fragment containing two expression cassettes was synthesized artificially (GenScript, China): one was for the plasma-membrane-targeted mTagBFP2-FRB and the other was for the ICR1- or ICR2-fused mCherry-FKBP12 (Fig ...
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bioRxiv - Immunology 2020Quote: ... Two commercially available ACE2-Fc proteins obtained from Genscript (Cat.No. Z03484-1) and Acrobiosystems (Cat.No ...
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bioRxiv - Microbiology 2021Quote: ... or AnfD were generated using the PolyExpress Silver package (two epitopes) from Genscript. Specificity of the antibodies was confirmed using recombinant NifD ...
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bioRxiv - Biochemistry 2021Quote: ... leaving two extra GS residues between the tag and the M125 residue (GenScript). Wild-type and the associated LonP1 mutants were expressed in BL21 (DE3 ...
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bioRxiv - Immunology 2023Quote: ... injection at two sites on the back with 100μg of MOG35-55 (GenScript) emulsified 1:1 in 100μl CFA (Chondrex Inc.) ...
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bioRxiv - Bioengineering 2019Quote: ... flanked by two cysteine residues (VPM peptide, GCRDVPMSMRGGDRCG, purity 96.9%, Mw 1696.96 Da, GenScript). Cells and/or soluble molecules ...
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bioRxiv - Systems Biology 2023Quote: ... Two constructs used for the initial multiplex CRISPRi/dCpf1 prototyping were synthesized by Genscript USA Inc (Piscataway ...
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bioRxiv - Plant Biology 2023Quote: ... Two codon-optimized ACD6Col-0 sequences were obtained and synthesized from gBlock synthesis (GenScript) and subcloned into pcDNA3.1 myc-6xHis or mGFP5-6xHis through the HiFi assembly method (NEB) ...
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bioRxiv - Developmental Biology 2020Quote: ... and was purified across two columns equipped with Ni2+ affinity resin (Ni-NTA Resin, GenScript) and MBP (PurKine MBP-Tag Dextrin Resin 6FF ...
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bioRxiv - Molecular Biology 2022Quote: ... SUMO4 knockout U2OSTrEx-FlpIn were generated using two different guide RNAs pSpCas9 (BB)-2A PURO (GenScript) plasmids that target the SUMO4 gene at nucleotides (relative to start codon ...
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bioRxiv - Synthetic Biology 2019Quote: ... and the melamine operon was amplified in two halves from a synthetic construct (GenScript, Hong Kong, Ltd.) using primers D98847023 and D98847024 (first half ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequences representing the two DGAT1 isoforms were synthesised and cloned into pcDNA3.1 by GenScript (New Jersey, USA). Co-transfection of cells with pMAXGFP plasmid (Lonza ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with two replicates for each antibody (LY-CoV016, LY-CoV555, REGN10987, and S309 [Genscript, Gene-to-Antibody service]) assay on different days ...
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bioRxiv - Molecular Biology 2023Quote: ... FYNB (NM002037.5) flanked by two gateway sites have been synthesized and cloned in PUC57 plasmid (GenScript, Hong Kong). These two clones were individually transferred by Gateway recombinational cloning into pDONR207 vector ...
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bioRxiv - Biochemistry 2022Quote: ... two polypeptides with the sequence MQDDLLMDKSKTGGGGASSSWNTHQ (with and without an N-terminal Dansyl group) were also obtained from Genscript. All the peptides were over 95% pure ...
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bioRxiv - Evolutionary Biology 2021Quote: ... This 338aa protein fragment was then expressed in E.coli and used to immunize two rabbits for antibody production by GenScript. ELISA titer > 1:128,000 and target protein fragment binding validation by western blot and cell line overexpression.
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bioRxiv - Genomics 2024Quote: The two recoded TALEN arms were ordered as synthetic pieces of DNA delivered into the pUC57-Mini vector (GenScript). The left recoded arm was cloned in pHR’CMVGFP_hRIF1(1924-2446)IREShygro3 (Addgene #23138 ...
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bioRxiv - Immunology 2024Quote: ... coli system by Genscript and supplied in protein storage buffer (50 mM Tris-HCl ...
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bioRxiv - Bioengineering 2021Quote: ... under two different RNP complexing conditions: 1) 10 mins at 25°C or 37°C with nuclease reaction buffer (GenScript) and 2 ...
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bioRxiv - Cell Biology 2021Quote: ... which was synthesized to contain 7 modified TetO elements flanked by two minimal CMV promoter sequences based on pTet-T2 sequences (GenScript) (49) ...
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bioRxiv - Bioengineering 2022Quote: ... The cassette containing the two-sgRNA array (targeting msfGFP and mScarlet) and the codon-optimized cas6 were purchased from Genscript and further cloned to the sgRNA expressing backbone ...
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bioRxiv - Immunology 2021Quote: ... This recombinant target panel consisted of two full-length viral structural proteins, S (FL, trimer-stabilized, LakePharma) and N (GenScript); two truncated S protein domains ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured at 5e5 cells/well with two peptide pools representing the full-length S protein at 1 μg/ml (Genscript) overnight in order to stimulate the cells ...
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bioRxiv - Immunology 2023Quote: ... TAP1 deficient 221-C*08:02 cells were generated by expression of HLA-C*08:02 in 221-Cas9 cells followed by transduction with two gRNA targeting TAP1 (Genscript)42 ...
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bioRxiv - Molecular Biology 2023Quote: ... approximately 25 μM positive-sense strand ssRNA synthesized by IVT was annealed with 25 μM each of the two RNA oligonucleotides (synthesized chemically by GenScript) corresponding to the two segments of the negative-sense strand ssRNA.
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bioRxiv - Evolutionary Biology 2020Quote: ... custom polyclonal antibodies were generated in rabbits against recombinant fragments corresponding to regions that significantly differ between the two AGOs (GenScript, USA).
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bioRxiv - Molecular Biology 2021Quote: ... elephas VTG amino acid sequence deduced in silico (Fig. 1A) was used to create two synthetic peptides (Fig 1B) (Genscript USA Inc.) to be employed for the production of specific anti-VTG antibodies (Twin Helix ...
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bioRxiv - Molecular Biology 2020Quote: ... ShCAST system was synthesized by GenScript according to the constructs described previously40 ...