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Citations for GenScript :
1 - 48 of 48 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 10Lμg of tissue lysates were resolved on a 4–12% Bris-Tris gel (M00668, GenScript) and transferred onto a nitrocellulose membrane ...
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bioRxiv - Microbiology 2020Quote: ... coli lysate (Genscript) at final concentration of 10 mg/mL to block anti-E ...
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bioRxiv - Microbiology 2023Quote: ... coli lysate (Genscript, USA). The diluted serum samples were incubated with the protein arrays overnight at 40C ...
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bioRxiv - Molecular Biology 2021Quote: ... coli lysate (GenScript, Piscataway, NJ) and incubated for 30 minutes on a shaker at RT ...
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bioRxiv - Immunology 2023Quote: ... coli lysate (GenScript, Piscataway, NJ) and incubated for 30 minutes on a shaker at room temperature (RT) ...
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bioRxiv - Biochemistry 2023Quote: The operon encoding LT from ETEC strains of porcine origin (also known as pLT) was synthesized by Genscript® with LT-IIB signal sequences ...
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bioRxiv - Immunology 2024Quote: ... coli lysate (GenScript, Piscataway, NJ, USA) to a final concentration of 10 mg/mL and preincubated at room temperature (RT ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of cell lysate was saved as an input control and the remaining cell lysate was incubated with Glutathione MagBeads (GenScript, L00327) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... coli lysates using glutathione-agarose or -sepharose (Sigma; GenScript) and Talon metal affinity resin (Clontech) ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysates were incubated with G1 affinity resin (Genscript) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Lysate was incubated with anti-DYKDDDDK G1 affinity beads (Genscript) for 3 hours at 4 °C and washed with 20 column volumes of purification buffer ...
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bioRxiv - Cancer Biology 2023Quote: Cell lysates were separated on an SDS-polyacrylamide gel (Genscript) and transferred to a nitrocellulose membrane using a wet transfer system (BioRad) ...
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bioRxiv - Bioengineering 2023Quote: ... We used gel-clot lyophilized amebocyte lysate tests (ToxinSensor, Genscript) to detect the presence of endotoxin above the detection threshold ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysates were applied to high affinity Ni-charged resin (Genscript, L00223) and eluted with increasing imidazole concentrations (150 – 750 mM).
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bioRxiv - Biochemistry 2023Quote: ... The cell lysates were separated by 4-20% SDS-PAGE (GenScript M00657) and transferred to a nitrocellulose membrane (0.45 µm ...
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bioRxiv - Cell Biology 2019Quote: ... quantified using a chromogenic limulus amebocyte lysate endotoxin assay kit (GenScript, Piscataway, NJ), were significantly below those necessary for activation of TLR4 (typically <0.05 ng/ml).
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bioRxiv - Cell Biology 2021Quote: ... Lysate was flowed over an Anti-FLAG resin (GenScript, Piscataway, NJ, US, L00432) column at 1 mL/min ...
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bioRxiv - Immunology 2022Quote: ... Lysates were loaded onto any-kD gradient ExpressPlus™ Page precast gels (GenScript). Proteins were transferred onto a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... This clarified protein lysate was then shaken with Ni-charged IMAC Magbeads (Genscript) for 1 hour to bind tagged proteins ...
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bioRxiv - Cell Biology 2024Quote: Protein lysates were incubated with 1 µg mouse anti-V5 antibody (Genscript A01724) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: Lysates were separated by electrophoresis on precast 12% Tris-Bis SDS-PAGE gel (Genscript) or 4-20% precast gels (BioRad) ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was then incubated with 125 µL of G1 Flag resin (Genscript) per 30 ml culture overnight at 4 °C with rotating before washing with 50 bead volumes of lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Whole worm lysates were then separated on 4-12% polyacrylamide gradient gels (GenScript, #M00654), transferred to membranes ...
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bioRxiv - Immunology 2021Quote: ... The lysate was immunoprecipitated using designated primary antibodies with protein G resin (GenScript, Piscataway, NJ), or anti-Flag M2 affinity agarose gel at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein lysates were loaded onto SurePAGE Bis-Tris 4-20% gels (Genscript) and transferred either to
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bioRxiv - Genomics 2023Quote: Cell lysates from HMLE shLuc and shM2 were separated by 4-20% SDS-PAGE (Genscript) and transferred to a PVDF membrane (10600023 GE) ...
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bioRxiv - Cell Biology 2024Quote: ... Clarified lysates were incubated with 1 mL Anti-DYKDDDDK (FLAG) Affinity Resin (Genscript; Piscataway, NJ) for 2 h at 4 °C and then washed with 10 column volumes (CVs ...
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bioRxiv - Cell Biology 2021Quote: ... Whole worm lysates were then separated on 4-12% polyacrylamide gradient gels (GenScript #M00652 and #M00654), transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were boiled for 5 minutes with 100 mM DTT and 1X LDS buffer (GenScript M00676) and run on NuPAGE 4-12% Bis-Tris gels (Thermo NP0335BOX ...
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bioRxiv - Biochemistry 2023Quote: Lysates prepared as described in respective protocols were separated on a SurePage Bis-Tris polyacrylamide gel (Genscript) and transferred to a nitrocellulose membrane using the semi-dry iBlot2 system (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... The Drosophila SARM1 TIR used for the cell lysates experiments was synthesized with codon optimization by Genscript Corp and then cloned by Gibson assembly into a pACYC backbone with a Twin Strep tag fused to the N-terminus (Supplementary file 3) ...
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bioRxiv - Biochemistry 2024Quote: ... Clarified lysate from 1 L of culture was incubated with 1 mL of equilibrated Ni-resin (Genscript) and washed with 30 mL wash buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... equal amounts of lysates were loaded and separated by SDS-PAGE using 4-12% SurePAGE Bis- Tris (GenScript) or 3-8% NuPAGE Tris-Acetate (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Lysate supernatants were incubated overnight at 4°C with anti-flag monoclonal antibody coated on agarose beads (Genscript). The beads were then washed five times in wash buffer (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... The clarified cell lysate was incubated with 2 ml pre-equilibrated Ni2+ charged magnetic resin purchased from GenScript. Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Serum LPS concentrations were measured with a ToxinSensor Chromogenic Limulus Amebocyte Lysate (LAL) Endotoxin Assay Kit (GenScript, Piscataway, NJ), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were then incubated with antibody (anti-Myc antibody 9E10 or anti-CycB3, from rabbit, custom-made by Genscript) for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Lysate from equal number of DRGs was loaded and separated by electrophoresis on 4-12% ExpressPlus gels (Genscript M41210), followed by transfer to a PVDF membrane ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting lysate was centrifuged at 12,000 x g to separate the soluble protein fraction and incubated with glutathione resin (GenScript) for 30 minutes while shaking at room temperature (RT) ...
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bioRxiv - Microbiology 2019Quote: ... and 500 μL of tissue culture media containing 500 pM GLP-1 (GenScript) added to the lower chamber ...
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bioRxiv - Neuroscience 2023Quote: ... FOLR1 was immunoprecipitated by overnight incubation of lysates with rabbit anti-Xenopus laevis FOLR1 polyclonal affinity purified antibody raised against the peptide KHQKVDPGPEDDLHC (custom made by GenScript), chemically cross-linked to protein G-agarose beads at 4°C on a mini-rotator ...
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bioRxiv - Cell Biology 2022Quote: ... 20-50 μg of protein lysate of each sample was loaded and separated on 4-12% Bis-Tris gels (Thermo Fisher or GenScript) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... After clearance by ultracentrifugation (45 min, 120,000 g) the lysates were incubated with either FLAG-antibody- coated beads (Genscript, L004332-5) or Strep-Tactin®XT 4Flow high capacity resin (IBA life sciences ...
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bioRxiv - Microbiology 2021Quote: The phage endotoxin concentration was measured using the chromogenic Limulus Amebocyte Lysate ToxinSensor™ kit (Genscript Corporation, NJ, USA, < 0.01 EU/ml) and the Staphylococcal enterotoxin (SET ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of GST-G12-like and His-AaCPR100A sonicated lysates were mixed with high-affinity GST resin (GenScript, Piscataway, NJ, USA), and then eluted ...
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bioRxiv - Immunology 2024Quote: ... identical amounts and volumes of protein lysate from either untransfected or transfected cells were added to 20 μl of anti-DYKDDDDK G1 Affinity Resin (GenScript, Cat. No. L00432), previously washed with TBS 0.1% Tween™ 20 (TBS-T) ...
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bioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions and plated in 24 well tissue culture plates at 3×106/well in in the presence of OT-I peptide (GenScript) and the following cytokines ...
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bioRxiv - Microbiology 2019Quote: ... the dissected thrips tissues that were only incubated with secondary antibody (without adding primary antibody) and the tissues incubated with each pre-immune mouse antiserum (GenScript) were used as negative controls ...