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Citations for GenScript :
1 - 50 of 75 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the QuickClean 5M PCR Purification Kit (GenScript, Piscataway, NJ, USA). NanoDrop ND-100 Spectrophotometer (NanoDrop Technologies ...
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bioRxiv - Immunology 2021Quote: ... PCR products were Sanger sequenced by GenScript, and sequences were analyzed using IMGT/V-QUEST (32).
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bioRxiv - Immunology 2020Quote: ... PCR products were Sanger sequenced by Genscript and sequences were analyzed using IMGT/V-QUEST (33) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were Sanger sequenced by Genscript.
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bioRxiv - Genomics 2023Quote: ... with CloneEZ PCR Cloning Kit (GenScript #L00339). The pLenti-GIII-EF1α-CBX3-Flag-HA ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplifications were performed with Taq DNA Polymerase (GenScript) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Plates were coated overnight with 100 ng/well of S1 (Genscript) in coating buffer (0.015M carbonate/bicarbonate buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... CCT isoforms were PCR amplified from synthetic vectors from GenScript. SpCas9 was PCR amplified from px4591 ...
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bioRxiv - Molecular Biology 2021Quote: ... Point mutations were introduced by the PCR CloneEZ method (GenScript). Plasmids were transformed in acrB-deficient MG1655 E ...
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bioRxiv - Plant Biology 2021Quote: ... The purified PCR amplicons were sequenced using Sanger sequencing (Genscript, NJ). The overlapping PCR amplicon sequences were aligned and comparative analysis with the WT sequences was used to identify mutations for each of the independent nec3 mutants.
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bioRxiv - Immunology 2020Quote: ... ELISA plates were coated with 200 ng/well CoV2 RBD protein (GenScript; Cat: Z03483) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were generated by PCR or DNA synthesis (Genscript, New Jersey) containing ∼500-1000 bp DNA flanking each gene and fused together ...
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bioRxiv - Microbiology 2021Quote: Microtiter plates (96-well; Thermo) were coated with 1 μg/mL S-2P protein (Genscript) corresponding to the S protein of the Wuhan-Hu-1 virus ...
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bioRxiv - Microbiology 2020Quote: ... hnRNPUL1 (NM_007040) and PEG10 (NM_015068.3) were PCR amplified from ORF clones (hnRNPL, GenScript #OHu14072 ...
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bioRxiv - Biophysics 2023Quote: ... FRET-labeled 207 bp DNA was prepared by PCR using labeled primers (GenScript) ATCGGACCC/iCy5N/ATACGCGGCC (forward primer) ...
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bioRxiv - Immunology 2019Quote: 96-well plates were coated overnight at 4°C with mouse anti-Avi-tag antibody (Genscript) at 2 μg/ml in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Templates for sgRNA transcription were generated by PCR amplifying synthesized fragments (IDT and Genscript) or by annealing a T7 primer oligo to a single stranded template oligonucleotide ...
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bioRxiv - Microbiology 2019Quote: ... Primer synthesis and the sequencing of PCR products or plasmids were performed by Genscript Biotech (Nanjing ...
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bioRxiv - Biochemistry 2023Quote: ... the PCR amplified sfGFP sequence was first subcloned into vector pUC57 containing synthesized (GenScript) C-terminus for 3C-STREP-KKCKK-GFP11 (pFMP249) ...
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bioRxiv - Genetics 2024Quote: ... and cloning into the pJR1-41XL vector116 with the CloneEZ PCR Cloning Kit (GenScript). Plasmids were checked by PCR for correct insert size and also by Sanger sequencing (Eurofins Genomics ...
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bioRxiv - Biochemistry 2024Quote: ... the coding sequences for the PINK1 constructs were PCR amplified using clone OHu25380D (Genscript) as a template and cloned into the vector pFB-6HZB (SGC ...
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bioRxiv - Immunology 2019Quote: 96-well ELISA plates were coated overnight at 4°C with mouse anti-Avi-tag antibody (Genscript) at 2 μg/ml in PBS ...
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bioRxiv - Immunology 2023Quote: ... Plates were coated with 500 ng/mL RBD or 1 μg/mL NP (GenScript, Piscataway, New Jersey), and heat-inactivated plasma (1:50 in blocking buffer ...
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bioRxiv - Biophysics 2021Quote: ... cyaA DNA was amplified from genomic DNA by PCR and cloned in pET-15b (GenScript) using AsuII and NcoI enzymes to generate plasmid pME14 ...
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bioRxiv - Plant Biology 2021Quote: ... Primers were designed with Real-time PCR (TaqMan) Primer and Probes Design Tool from GenScript wesite (www.genscript.com/tools/real-time-pcr-taqman-primer-design-tool ...
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bioRxiv - Bioengineering 2022Quote: ... and gene expression was examined by real-time PCR using primer pairs (Genscript, Nanjing, China) and SYBR Green (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The resulting DNA was used as template for PCR amplification using Taq DNA polymerase (Genscript). DNA fragments resolved with electrophoresis of 1.2% Agarose gel purified using the Gel Extraction Kit (Qiagen ...
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bioRxiv - Genomics 2023Quote: The ACE2 coding sequence was synthesized by PCR amplification from the pUC57-ACE2 plasmid (GenScript). We included a NheI restriction site at the 5’ terminus and a PmeI restriction site at the 3’ terminus ...
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bioRxiv - Immunology 2022Quote: ... total splenocytes were seeded at 0.5×106 cells per well in a 24-well plate and stimulated with 0.1 μM OVA257-264 (Genscript RP10611) peptide and 20 ng/ml recombinant murine interleukin-2 (IL-2 ...
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bioRxiv - Immunology 2022Quote: ... 2 × 106 splenocytes from each animal were transferred in a round bottom 96 well plate (200 µl volume) and ex vivo restimulated with 1 µg/ml of the lmon_0149 peptides YSYKFIRV (GenScript) and QVFEGLYTL (made in house by solid phase synthesis ...
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bioRxiv - Immunology 2023Quote: ... 5×105 cells per well (6 well plate) were stimulated with 100 ng/mL of IFN-γ (Z02916, Genscript) for 0 h ...
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bioRxiv - Microbiology 2019Quote: ... cdeR and cseR were made by introducing PCR-generated amplicons or synthetic gene fragments (IDT or Genscript) into pEX18Gm-derived delivery plasmid (39) ...
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bioRxiv - Physiology 2022Quote: ... The purified PCR products and linearized vectors were assembled using a GenBuilder Plus kit (Genscript, L00744-10) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 N gene was PCR amplified from plasmids pUC57-SARS-CoV-2-N (GenScript) using forward primers with T7 promoter and reverse primers with poly(T)34 sequences ...
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bioRxiv - Microbiology 2023Quote: ... The SARS-CoV-2 N gene was PCR amplified from plasmids pUC57-SARS-CoV-2-N (GenScript) using forward primers with T7 promoter and reverse primers with poly(T)34 sequences ...
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bioRxiv - Immunology 2021Quote: ... One million of freshly isolated splenocytes were seeded into the precoated plates and stimulated with S1 and S2 peptides pools (GenScript) with a final concentration of 1 μg/ml of each peptide diluted in RPMI-1640 supplemented with 10% FBS and incubated for 48 hours at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions and plated in 24 well tissue culture plates at 3×106/well in in the presence of OT-I peptide (GenScript) and the following cytokines ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were plated in a 6 well plate and co-transfected with 1 μg of pUC57-NASP-FKBP12F36V (by Genscript) and 2 μg of pSpCas9(BB)-2A-Puro-NASP-sgRNA_V2.0 (#62988 ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were coated with 2 μg/mL of recombinant Karp type-specific antigen 56 (TSA56, generated by Genscript) in PBS and blocked with 1% BSA ...
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bioRxiv - Bioengineering 2019Quote: ... The resulting PCR products were analyzed by agarose gel electrophoresis and DNA sequencing (GenScript Biotech Corp., Nanjing, China).
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bioRxiv - Cancer Biology 2022Quote: Human RET-GFP and RET712-1114-GFP were cloned by PCR using a cDNA clone (GenScript Biotech; NM_020975.6) as template ...
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bioRxiv - Plant Biology 2019Quote: ... PCR products were run in 2% agarose gel electrophoresis using a 100 bp DNA ladder (GenScript; CAT: M102O).
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR products were analysed by agarose gel electrophoresis and confirmed by Sanger sequencing (GenScript, Nanjing, China). The genome editing plasmids were cured following the method we previously developed (Zheng et al ...
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bioRxiv - Immunology 2023Quote: ... Raw values were normalized to a synthetic standard on each plate (VHH72-Fc by NRC for spike/RBD or an anti-N IgG from Genscript, #A02039). The relative ratios were further converted to BAU/mL using the WHO International Standard 20/136 as a calibrant (33 ...
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bioRxiv - Neuroscience 2019Quote: ... The GtACR1-EYFP fragment from the p7-GtCAR1 plasmid was swapped in using CloneEZ® PCR Cloning Kit (GenScript) for the myr::GFP fragment in pJFRC177 and the sequence was verified (GenScript) ...
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bioRxiv - Molecular Biology 2019Quote: ... MCM7 or CDC45 were amplified by PCR using Phusion polymerase (Thermo) from pcDNA3.1 plasmids encoding the cDNA of each protein (MCM plasmids from Genscript). The PCR products were then cloned into the pGBKT7-BD or pGADT7-AD plasmids ...
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bioRxiv - Microbiology 2020Quote: ... hnRNPUL1 (NM_007040) and PEG10 (NM_015068.3) were PCR amplified from ORF clones (hnRNPL, GenScript #OHu14072; hnRNPUL1, Origene #RC200576; PEG10, GenScript #OHu101111) and the products were cloned into the mammalian expression plasmid pCAGGS ...
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bioRxiv - Microbiology 2022Quote: All constructs were PCR amplified from a codon optimized gene block encoding the coding sequence of human DYRK1A (GenScript) using Q5 High-Fidelity DNA Polymerase with GC enhancer buffer (New England Biolabs) ...
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bioRxiv - Bioengineering 2022Quote: ... by means of overhang PCR and was cloned via traditional restriction/ligation into a pET28a(+) plasmid (ordered from GenScript) coding for the C-terminal flexible linker and small BiT (SB ...
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bioRxiv - Bioengineering 2020Quote: ... for detection of anti-L and anti-D antibodies plates were coated with either L-MMP peptide or D-MMP peptide resepcitvely (GenScript; sequence above) Serum samples were tested at a 1:500 dilution followed by incubation with alkaline phosphatase-labeled goat anti-mouse IgG1 or IgG2a ...