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Citations for GenScript :
451 - 500 of 907 citations for Recombinant Human ERN1 Protein GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Human codon-optimized HSPH1 fused C-terminally to a Myc-tag was expressed from pcDNA3.1 (Genscript). cDNA encoding wild-type human ubiquitin containing an N-terminal HA-tag was expressed from pRK5-HA (Addgene) ...
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bioRxiv - Immunology 2022Quote: ... Plasmids encoding human codon-optimized spike genes with the desired mutations were purchased (GenScript, Piscataway, NJ). Supernatants containing pseudoviruses were collected 48 h post-transfection ...
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bioRxiv - Cell Biology 2021Quote: Synaptopodin A was synthesized by Genscript using the coding sequence of human synaptopodin (NCBI Reference Sequence: NP_009217.3) and was subcloned by Genscript into HINDIII and Xho1 sites of the blasticidin-selectable mammalian expression vector pcDNA6 myc-HisA (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and non-human private were determined by the virus surrogate neutralization kit (cat # L00847, Genscript, Singapore). The percent of neutralizing virus in sera were determinded according to the manufacturer’s protocol
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bioRxiv - Biophysics 2020Quote: The Rh-PDE (full) gene (NCBI Gene ID: 16078606) was synthesized after human codon optimization (GenScript), as described previously13 ...
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bioRxiv - Biochemistry 2021Quote: Codon optimized constructs of the truncated versions of human Syx were designed and obtained from GenScript. Fusion constructs were generated as described previously(48 ...
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bioRxiv - Cell Biology 2022Quote: Three subunits of the human mTORC1 complex (mTOR, Raptor, mLST8) were codon-optimized and synthesized (GenScript). The mTOR gene was cloned into a pCAG vector without a tag ...
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bioRxiv - Immunology 2022Quote: ... The cells were cultured for 24 hours prior to being treated with: Human IL11 (UniProtKB:P20809, GenScript), human TGFβ1 (PHP143B ...
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bioRxiv - Immunology 2022Quote: Antibody heavy and light chain genes were optimized for human cell expression and synthesized by GenScript. VH and VL were inserted separately into plasmids (pCMV3-CH ...
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bioRxiv - Microbiology 2024Quote: ... The murine-human chimeric heavy and light chain sequences were then cloned into pGenDONR by GenScript, with a P2A sequence added between the heavy and light chain sequences to create the pGenDONR-IgG H1-P2A-Ig(λ ...
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bioRxiv - Neuroscience 2024Quote: ... 2000) was codon optimized for human cells and synthesized into a pcDNA 3.1(+) vector by Genscript. Codon-optimized G5A and Gqi5/9 sequences as well as cloning primers and further details are provided in Supplementary file 7.
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bioRxiv - Cancer Biology 2024Quote: ... Control (#2) and p53 gRNA (#4) vectors targeting human TP53 (GenScript, pLentiCRISPR v2, Piscataway, NJ, USA) were used to homozygously delete the TP53 gene in H1975 cells as per manufacturer instructions.
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bioRxiv - Microbiology 2023Quote: ... Dabie Bandavirus (DBV) glycoprotein Gn gene (GenBank NC_018138.1) was codon-optimized for human codon usage (Genscript) and cloned into the expression vector ...
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bioRxiv - Neuroscience 2024Quote: ... and a plasmid encoding human Kv5.1 with a C-terminal GFP tag was generated by Genscript. The Kv5.1BBS construct was generated at Genscript by inserting a bungarotoxin-binding site (GGWRYYESSLLPYPDGG ...
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bioRxiv - Neuroscience 2023Quote: ... The construct consisting of human APP770 cDNA in the pcDNA3.1 backbone was custom made (GenScript, Piscataway) and then sequenced ...
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bioRxiv - Molecular Biology 2020Quote: ... Polyreactivity was quantified by detecting bound IgG using an HRP-conjugated anti-human IgG secondary antibody (Genscript) and SuperSignal ELISA Femto Maxiumum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... DNA polynucleotides encoding the BCCR opsin domains optimized for human codon usage were synthesized (GenScript, Piscataway, NJ) and cloned into the mammalian expression vector pcDNA3.1 (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Three human codon-optimized As-NF-κB (named 1, 2, and 3) cDNAs were synthesized by GenScript based on sequences from the transcriptome of A ...
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bioRxiv - Biochemistry 2022Quote: ... A peptide corresponding to the human CRX homeodomain (amino acids 39 to 98) was synthesized by Genscript.
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bioRxiv - Biochemistry 2022Quote: Codon optimized human wild-type full-length (FL) APE1 in a pet28a vector was purchased from GenScript. The E96A/D210N ...
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bioRxiv - Immunology 2022Quote: ... Human codon-optimized cDNA encoding SARS-CoV-2 spike glycoproteins of various strains were synthesized by GenScript and cloned into eukaryotic cell expression vector pcDNA 3.1 between the BamHI and XhoI sites ...
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bioRxiv - Biochemistry 2022Quote: ... and human CDC73 sequences with an N-terminal Flag tag were synthesized and sequences confirmed by GenScript. The human ubiquitin sequence with an inserted N-terminal cysteine residue was synthesized by Eurofins ...
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bioRxiv - Immunology 2023Quote: ... pEF1-K6a (no tag) was constructed using a pUC57 vector encoding human K6a (codon-optimized by Genscript) as a PCR template to generate a full length human K6a DNA fragment terminated by a stop codon ...
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bioRxiv - Biochemistry 2023Quote: ... The cDNA of human STK25 isoform 1 (RefSeq accession no. NM_001271977.2) was cloned into the pcDNA3.1+ vector (GenScript). The Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Cell Biology 2023Quote: The open reading frame of full-length human GPNMB (NM_001005340.2) cloned into pcDNA3.1-C-EGFP was purchased from GenScript. pcDNA3.1-GPNMB-EGFP-G375V ...
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bioRxiv - Microbiology 2024Quote: ... Human ACE2 (GenBank accession: NM_021804.2) with a C-terminal Flag tag was synthesized by GenScript (Piscataway, NJ) and pCSDest-TMPRSS2 (Addgene Plasmid # 53887 ...
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bioRxiv - Immunology 2024Quote: Antibody heavy and light chain genes were first optimized for human cell expression and synthesized by GenScript. VH and VL segments were separately inserted into plasmids (pCMV3-CH ...
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bioRxiv - Immunology 2024Quote: Antibody heavy and light chain genes were first optimized for human cell expression and synthesized by GenScript. VH and VL segments were separately inserted into plasmids (pCMV3-CH ...
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bioRxiv - Biophysics 2024Quote: The codon-optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683 ...
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bioRxiv - Bioengineering 2021Quote: ... Codon-optimized cDNAs containing the effector protein RfxCas13d were synthesized (Genscript) and assembled into modified pET28 vectors by NEBuilder HiFi DNA assembly (E2621 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were separated in 4-20% gradient precast PAGE gels (Genscript) and stained by Coomassie blue.
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bioRxiv - Biochemistry 2020Quote: ... Soluble protein was further purified via glutathione agarose chromatography (GenScript # L00206) with gravity flow at room temperature in fresh buffer B ...
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bioRxiv - Cell Biology 2021Quote: ... supernatants were precleared with protein A-sepharose beads (GenScript, Piscataway, NJ). Precleared supernatants were then incubated overnight at 4°C with 5μg of anti-NM IIA pAb or control rabbit immunoglobulin ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were resolved on 10% SDS-polyacrylamide gels (GenScript, M00666) and transferred to polyvinylidene fluoride membranes (Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... centrifuged and purified by affinity chromatography with protein A resin (Genscript), and subjected to desalt in a composed buffer 50 mM NaPO4 ...
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bioRxiv - Biochemistry 2021Quote: ... SMT (SUMO-like tag) fusion protein in a pET28a vector (Genscript). Quick Change mutagenesis was performed to generate the W611A mutant of hP13 ZnF5-WWE1-WWE2 ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were separated by 4– 12% gradient SDS-PAGE (GenScript) and blotted onto nitrocellulose or PVDF membranes ...
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bioRxiv - Evolutionary Biology 2022Quote: Protein G (5 μg/ml, 50 μl/well; Genscript, China, Z02007) was diluted to 5 μg/ml with PBS (0.01 M ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV (NP_828851.1) and MERS (AFS88936.1) Spike proteins were synthesized (GenScript) and cloned into pcDNA3.1 ...
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bioRxiv - Microbiology 2021Quote: LAD2 cells (3×105) were exposed to Spike-RBD protein (Genscript) (5 μg/mL) ...
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bioRxiv - Immunology 2022Quote: ... were coated with 100 ng/well Delta-S1 protein (GenScript, China) or 250 ng/well purified Delta-S protein in PBS and incubated at 4 °C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The DNA encoding this fusion protein was commercially synthesized (GenScript Inc) and cloned into pET15b bacterial expression vector at Nco I/BamH I cloning sites ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was transferred to a PVDF membrane using eBlot L1 (Genscript). Blocking was performed with 5% milk in PBST (PBS + 0.1% TritonX-100 ...
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bioRxiv - Microbiology 2022Quote: ... An anti-SARS-2 nucleocapsid protein rabbit antiserum (generated by GenScript) was used at a 1:1000 dilution to detect specific anti–SARS-2 immunoreactivity using the Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics ...
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bioRxiv - Neuroscience 2024Quote: ... An additional plasmid encoding green fluorescent protein (GFP; pCAG-GFP; GenScript), was used to label successfully transfected cells ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 N and S1 proteins were obtained from Genscript and Sino Biological.
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bioRxiv - Microbiology 2023Quote: ... The proteins were separated with an ExpressPlusTM PAGE gel (Genscript, M42012) and transferred to a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... smegmatis gene MSMEG_3935 encoding RafH protein was commercially synthesized from GenScript and cloned in the pET-28a(+ ...
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bioRxiv - Biochemistry 2023Quote: ... followed by staining using the eStain L1 Protein Staining System (GenScript). PAGE-MASTER Protein Standard Plus (GenScript ...
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bioRxiv - Immunology 2023Quote: CPG2 and β-Lac proteins were produced and purified by GenScript as previously described (7) ...