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Citations for Singer Instruments :
1 - 50 of 57 citations for Rat Colony Assay Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Both assays take advantage of the RoToR HDA colony-pinning robot (Singer Instruments), which can pin out yeast on agar plates in a 96-well arrangement ...
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bioRxiv - Synthetic Biology 2021Quote: ... each individual colony was isolated using the Rotor HDA colony picker (Singer instruments, UK). Using the colony picker ...
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bioRxiv - Genetics 2020Quote: ... deletion library was arrayed on solid agar EMM media plates in in 384 colony/plate format using a RoToR HDA robot (Singer Instruments). After two days of growth plates were scanned and raw colony sizes extracted using gitter R package [54] ...
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bioRxiv - Genetics 2022Quote: ... and a gene deletion were performed by sequential pinning of yeast colonies on appropriate selective media using a RoToR pinning robot (Singer Instruments) as described (Baryshnikova et al. ...
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bioRxiv - Genomics 2023Quote: Extracts from commercial spontaneous fermentations sourced from wineries within Australia were plated on WL media containing 25 µg/mL chloramphenicol and then arrayed on YPD media +25 µg/mL chloramphenicol in 96-well format using a PIXL automated colony-picker (Singer Instruments) or by hand ...
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bioRxiv - Molecular Biology 2023Quote: ... methodology 33,34 by sequential pinning of yeast colonies on agar plates with appropriate selective media using a pinning robot (Rotor, Singer Instruments). Each cross was performed in 4 technical replicates arranged next to each other in a 2×2 group ...
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bioRxiv - Genetics 2021Quote: ... Strains were arranged into 384-colony format using a RoToR HDA colony-pinning robot (Singer Instruments), including a 96-colony grid of wild-type 972 h- strains for plate normalization (Kamrad ...
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bioRxiv - Genetics 2020Quote: ... Cells for the colonies were transferred to two agar plates containing the test media using an automated pinning robot (Singer Instrument ROTOR HDA). This allows for a total of four replicates per strain and per condition ...
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bioRxiv - Microbiology 2024Quote: ... Colonies were picked either using a disposable loop or a PIXL colony picking robot (Singer Instruments, PIX-001). When picking by hand ...
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bioRxiv - Cell Biology 2020Quote: ... Library was spotted on plates in 1536-colony format using a ROTOR colony pinning robot (Singer Instruments, Somerset, UK). Each library strain had four technical replicates spotted next to each other (2×2) ...
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bioRxiv - Molecular Biology 2019Quote: ... Mating was performed on 1536-colony format YPD plates using a RoToR bench top colony arrayer (Singer Instruments; Somerset, UK). Resulting diploids were selected for deletion/DAmP libraries and ER-GFP markers KanR and URA3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Colonies were picked manually (sublibraries 1 and 5 only) or using a PIXL robotic colony picker (Singer Instrument Company, Somerset, UK) at the Stanford University School of Medicine Genome Technology Center (Palo Alto ...
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bioRxiv - Microbiology 2023Quote: ... An ordered PA14 transposon mutant library was pinned from source plates in 1536 colony density onto the rectangular agar plates using a ROTOR HDA robotic colony replicator (Singer Instruments). The screen was performed in duplicates using different source plates ...
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bioRxiv - Synthetic Biology 2022Quote: A PIXL colony picker (Singer Instruments, Roadwater, United Kingdom) was used to select individual colonies from the transformation plates using the 490-510 nm (cyan ...
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bioRxiv - Cell Biology 2022Quote: ... we used a RoToR benchtop colony arrayer (Singer Instruments). In short ...
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bioRxiv - Cell Biology 2019Quote: ... and PhenoSuite colony counting and analysis software (Singer Instruments). Colony growth on selection plates was scored and compared to colony growth on MATa control plates ...
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bioRxiv - Cell Biology 2020Quote: ... we used a RoToR bench top colony arrayer (Singer Instruments). In short ...
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bioRxiv - Bioengineering 2023Quote: ... colonies were replicated by stamping using a ROTOR robot (Singer Instruments, UK) to new plates as well as plates containing 200 mg L-1 amido-black for fluorescence screening as previously described
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bioRxiv - Genetics 2024Quote: The plates were imaged with the PhenoBooth Colony Counter (Singer Instruments, UK) after 72 hours of incubation and the size of the individual colonies was used as a proxy for fitness ...
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bioRxiv - Cell Biology 2021Quote: ... A RoToR bench-top colony array instrument (pinning robot; Singer Instruments, Somerset UK) was used to pin the libraries ...
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bioRxiv - Cell Biology 2022Quote: ... and libraries were handled using a RoToR bench-top colony arrayer (Singer Instruments). Briefly ...
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bioRxiv - Genetics 2021Quote: ... The colony sizes were calculated in pixels using Phenosuite software (Singer Instruments, UK) and the heat maps of the phenotypic behaviours were constructed using the R shiny app (https://kobchai-180shinyapps01.shinyapps.io/heatmap_construction/) ...
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bioRxiv - Cell Biology 2019Quote: ... 2006) in high-density format using a RoToR bench top colony arrayer (Singer Instruments).
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bioRxiv - Cell Biology 2019Quote: ... All copying of yeast colonies was performed on a Rotor robot (Singer Instruments, UK).
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bioRxiv - Genetics 2022Quote: ... in a density of 1536 colonies per plate using the RoToR HDA system (Singer Instruments, UK). Each single deletion strain was plated in quadruplicates allowing replicates for consistent SGA analysis ...
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bioRxiv - Genetics 2023Quote: ... plates containing 1536 colonies were pinned onto YES plates using a RoToR arraying robot (Singer Instruments) and incubated for 24 hours at 32°C ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast manipulations in high-density format were performed on a RoToR bench top colony arrayer (Singer Instruments). In short ...
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bioRxiv - Genetics 2024Quote: We obtained all the colony size of the mutants using the Phenobooth (Singer Instruments Ltd, Somerset, UK). The colony sizes of both double and single mutants were normalized per plate ...
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bioRxiv - Bioengineering 2022Quote: ... Fluorescent mVenus expression intensities were analyzed by picking primary transformant colonies using a PIXL robot (Singer Instruments, UK) to 384 colonies/plate layout on manufacturer supplied rectangular Petri dishes ...
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bioRxiv - Cell Biology 2021Quote: ... To perform the SGA in a high-density format we used a RoToR benchtop colony arrayer (Singer Instruments). In short ...
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bioRxiv - Molecular Biology 2023Quote: Yeast strains were assembled in 1536-colony format on agar plates using a pinning robot (Rotor, Singer Instruments). For each tFT strain ...
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bioRxiv - Cell Biology 2020Quote: ... The bait and prey colonies were mated on YPD plates using a RoToR HDA-Robot (Singer Instruments Co. Ltd) and incubated overnight at 30°C ...
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bioRxiv - Cell Biology 2019Quote: Synthetic Genetic Array (SGA) analysis was performed according to (Tong and Boone, 2007) using a colony pinning robot (Singer Instruments). The tcb1/2Δ query strain (WKY330 ...
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bioRxiv - Cell Biology 2022Quote: Cells were moved from agar plates into liquid 384-well plates using the RoToR bench-top colony arrayer (Singer Instruments). Liquid cultures were grown overnight in synthetic medium with 2% glucose (SD ...
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bioRxiv - Cell Biology 2022Quote: Cells were moved from agar plates into liquid 384-well plates using a RoToR bench-top colony arrayer (Singer Instruments). Liquid cultures were grown overnight in low biotin 0.512nM d-biotin (sigma ...
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bioRxiv - Genomics 2019Quote: ... strains were grown overnight on liquid YPD medium then pinned onto a solid SC medium with a 1,536 colony per plate density format using robot assisted pinning with ROTOR™ (Singer Instrument) and incubated overnight at 30°C ...
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bioRxiv - Cell Biology 2019Quote: ... the CerS3 strain was pinned onto 1536-format arrays of mutant colonies on YPD agar using a pinning robot (Singer Instruments) and incubated at room temperature for 1 day to induce mating ...
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bioRxiv - Genetics 2020Quote: ... cells were plated to YPD at 23°C at which time individual colonies were cherry-picked into 96-well plates to allow for automated pinning using the Singer ROTOR (Singer Instruments). Next ...
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bioRxiv - Microbiology 2022Quote: ... colonies were pinned on the antibiotic-containing plates and once on a vehicle control plate at 1536 colony density using the ROTOR HDA (Singer Instruments). This experiment was done in duplicate using two different source plates ...
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bioRxiv - Synthetic Biology 2023Quote: The images of grown colonies were captured and segmentized by PIXL with camera resolution of 5MP (2448 × 2048) (Singer Instruments, UK). The PIXL software segments the colonies in different manners based on four algorithms (background subtraction ...
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bioRxiv - Bioengineering 2023Quote: ... Up to 384 colonies per transformation event were transferred to TAPhi-NO3 agar plates using a PIXL robot (Singer Instruments, UK). After a further three days ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 µL of saturated cultures were dispensed into a sterile 96-well plate and subsequently pinned to 384 density on YPG agar using a Singer RoToR colony pinning robot (Singer Instruments). Plates were grown at room temperature until colonies formed (24-48 hours) ...
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bioRxiv - Genomics 2024Quote: ... was crossed with strains harboring the ura4+ reporter gene at different heterochromatic loci by using RoToR HDA colony pinning robot (Singer Instruments). To enable selection of the ura4+ reporter under conditions of transcriptional repression ...
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bioRxiv - Cell Biology 2021Quote: ... query and library strains were handled in high-density format (384-1536 strains per plate) using a RoToR bench-top colony arraying instrument (Singer Instruments, UK). In short ...
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bioRxiv - Cell Biology 2019Quote: ... Strains were inoculated from 384-colony agar plates to 384-well liquid cultures using a RoToR HDA robot (Singer Instrument Company Limited). The cultures were grown for 36 hours to saturation in a DTS-4 microplate thermoshaker (Appropriate Technical Resources) ...
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bioRxiv - Microbiology 2021Quote: ... the Hfr chloramphenicol resistant Lpp+21 strain was arrayed in 384-colony density on LB agar containing chloramphenicol using the Singer rotor HAD (Singer Instruments, United Kingdom). Similarly ...
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bioRxiv - Cancer Biology 2022Quote: ... and pinned in quadruplicate to achieve a 384-colony density (i.e. 4 technical replicates for each position of the microtiter plate) using a Singer RoToR robot (Singer Instruments, Inc. Somerset UK). Strains were grown on YES solid agar media with the indicated camptothecin concentrations ...
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bioRxiv - Systems Biology 2020Quote: The Chlamydomonas mutant collection12 was maintained by robotically passaging 384-colony arrays to fresh medium using a Singer RoToR robot (Singer Instruments, 704 Somerset, UK). The mutant collection was grown on 1.5% agar Tris-Acetate-Phosphate (TAP ...
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bioRxiv - Microbiology 2024Quote: ... A volume of 45 mL of media was poured into SBS PlusPlates (Singer Instruments, UK) and left to set on an even surface.
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bioRxiv - Molecular Biology 2022Quote: ... Solid media was cast in polystyrene plates from a single batch (Singer Instruments, SBS-format PlusPlates). Each plate was cast on top of the same perfectly level surface with precisely 50 mL of synthetic grape must medium at 50 C ...