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Citations for Takara Bio :
1 - 50 of 874 citations for Upstream stimulatory factor 2 USF2 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The upstream and downstream regions adjacent to the binding site were amplified using Advantage 2 polymerase (Takara Bio, Shiga, Japan) from 3T3-L1 genomic DNA and cloned into the HR110-PA-1 vector (System Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... These fragments were cloned into pUC19 with the HYG resistance cassette upstream of pCTR4-2 with InFusion cloning (Takara, Cat#638911). Plasmids were isolated from individual clones and confirmed by digestion with HindIII (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... upstream and downstream flanking regions (approximately 1,000 bp) were amplified using PrimeStar HS (Takara Bio) and cloned into the vector pCE along with a 30 bp site specific spacer sequence (in the gene to be deleted ...
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bioRxiv - Immunology 2021Quote: ... Fgf9 cDNA was cloned upstream of the IRES-eGfp-SV40 polyA cassette (pIRES2-eGfp vector, Clontech) in the pTRE2 vector (Clontech)49 ...
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bioRxiv - Biochemistry 2021Quote: ... Coding sequence for RIIα was amplified from the library using primers Prkar2a_F & Prkar2a_R and inserted upstream of the IRES2 sequence in pIRES2-GFP (Clontech) using EcoRI and BamHI entry sites ...
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bioRxiv - Plant Biology 2021Quote: ... The CaMV 35S promoter was then replaced by the HB40 native promoter (1,859 bp upstream translation start site) using In-Fusion (Takara) to generate the HB40:HB40-GFP construct ...
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bioRxiv - Molecular Biology 2024Quote: To generate APRF1pro:APRF1-mVENUS and TOPP4pro:TOPP4-3xFLAG the genomic region of both genes including a region of around 1.5 kb upstream their transcription start sites were amplified by PCR and cloned by the InFusion cloning (Takara) into the pCAMBIA1300 in frame with the coding sequences of mVENUS or the 3xFLAG peptide ...
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bioRxiv - Plant Biology 2023Quote: ... HRP substrates (Takara) and an iBrightTM CL750 Imaging System were used for immunodetection.
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bioRxiv - Microbiology 2021Quote: The complementing strain was constructed by amplifying either nyxA or nyxB and their corresponding promoter region (500 bp upstream) with the PrimeStar DNA polymerase (Takara). Insert and pmini-Tn7 (47 ...
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bioRxiv - Microbiology 2019Quote: ... The complementing strain was constructed by amplifying BspL and its promoter region (500 bp upstream) with the PrimeStar DNA polymerase (Takara) using the following primers ...
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bioRxiv - Plant Biology 2020Quote: A reporter plasmid was made by cloning the 76bp AG intron sequence upstream of the lacZ gene in the vector pLacZi (Clontech). The yeast reporter strain was made by integration of the linearized AG intron reporter plasmid into the yeast strain YM4271 ...
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bioRxiv - Plant Biology 2022Quote: ... and pMKMM21 (3.5 kb upstream SYP12A:5’UTR:miniTurbo- Myc-SYP12A:3’UTR) were generated by in-fusion cloning (HD enzyme mix; Takara Bio). Gateway binary vectors pMKMM22 and pMKMM23 for the expression of miniTurbo-Myc- MpSYP13B and miniTurbo-Myc-MpSYP12A were generated by LR-recombination (LR clonase ...
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bioRxiv - Plant Biology 2022Quote: ... we amplified the genomic fragment of the 4,194 bp upstream region of the translational initiation site from Tak-1 gDNA using PrimeSTAR Max DNA polymerase (Takara Bio), subcloned it into pENTR1A at the EcoRI restriction sites using the In-Fusion HD Cloning Kit and then transferred it into the destination vector pMpGWB10465 using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... the GST gene from the pEGKG plasmid (29) was amplified by PCR and inserted immediately upstream of GFPS65T using the In-Fusion HD cloning kit (Takara).
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bioRxiv - Plant Biology 2021Quote: ... MEL1 and lacZ) under distinct GAL4 upstream activating sequences as described in Matchmaker GAL4 Two-Hybrid System 3 & Libraries User Manual (Clontech). The transformants were grown on synthetic dropout (SD ...
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bioRxiv - Cell Biology 2021Quote: ... pLPCX-IRES-eGFP was generated by cloning a fusion PCRP consisting of the encephalomyocarditis virus internal ribosomal entry site (EMCV-IRES) upstream of the eGFP coding sequence flanked by EcoRI and NotI sites into the cognate sites of pLPCX (Clontech). A DNA gene block encoding the vaccinia virus Wisconsin strain K3L fused to the C-terminus of mNeonGreen by a GSGS linker and hosted into the expression vector pTwist Lenti SFFV puro WPRE was obtained commercially (Twist Bioscience) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 2019) upstream of an egfp coding sequence and an SV40 polyadenylation sequence using In-Fusion Snap Assembly master mixes (Takara). To prepare the injection mixtures ...
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bioRxiv - Microbiology 2024Quote: ... and the Tn7 site homologous fragments (upstream and downstream) were amplified from AB5075 from genomic DNA using the PrimeStar polymerase (Takara).
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bioRxiv - Immunology 2021Quote: ... and F1 and F2 arms homologous to upstream and downstream sequences of the A41L ORF using in-fusion cloning (Takara Bio). The resultant spike transfer cassette was linearized by NotI and transfected into the MCL for homologous recombination with SCV ...
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bioRxiv - Plant Biology 2019Quote: ... 2.5 kb of promoter sequences were isolated from genomic DNA and inserted upstream of the ß-glucoronidase gene of pCambia1301 vectors using the In-Fusion Cloning Recombinase kit (Clontech). The constructs were transformed into Agrobacterium tumefaciens strain GV3101 by electroporation ...
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Lateral organ diversification in plants mediated by the ALOG protein family of transcription factorsbioRxiv - Plant Biology 2019Quote: ... an MpTAW1 genomic fragment with a 10 kb upstream region and a 3 kb downstream region was amplified by PCR using Prime STAR GXL polymerase (TaKaRa, Japan) and subcloned into pENTR/D-TOPO (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... the V5-tag sequence followed by a Stop codon was introduced upstream of the IRES in the pLVX-IRES-Neo vector (Clontech, Addgene), using annealed oligo-cloning ...
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bioRxiv - Cell Biology 2023Quote: ... templates were constructed by cloning the 1.5 kb upstream and downstream homology arms into the pPD95.77 plasmid using the In-Fusion Advantage PCR cloning kit (Clontech, Cat. #639621). Fluorescence tags were inserted into the constructs with a flexible linker before the stop codons ...
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bioRxiv - Biochemistry 2023Quote: ... The fusion between the core of the AGA1 promoter (-150 to 0) and the STL1 upstream activation sequence (-800 to -163) was generated by In-Fusion cloning (Takara Bio) into a plasmid containing the 24xPP7 stem loops ...
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bioRxiv - Developmental Biology 2022Quote: ... A synthetic CRE sequence flanked by AttB1-AttB2 sites was cloned (by Eurofins) upstream of a IRES2-DsRed-express2 cassette (plasmid pIRES2-DsRed-express2 from Clontech/SakaRa, #632540), producing a plasmid called p4 containing AttB1-CRE-AttB2_IRES2-DsRed-express2 ...
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bioRxiv - Plant Biology 2024Quote: ... equilibrated with 2 μg of anti-GFP antibody (Takara Bio, 632381). Beads were washed for 2 x 10 mins in low salt wash buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The isolation of the upstream genome sequences of gnrh1 and gnrh3 of piranha was performed using the Universal GenomeWalker ™ kit (Takara Bio USA) according to the instructions ...
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bioRxiv - Plant Biology 2023Quote: ... the genomic sequence from the MpRKD promoter region (3827 bp upstream from start codon) to the 3’UTR was amplified by PCR with PrimeSTAR HS DNA polymerase (TaKaRa Bio, Kusatsu, Japan) and the primers IF-Hind-pRKD-F and IF-Sal-gRKD-R ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR-amplified enhancer-containing fragments were tandemly inserted into the upstream region of the original promoter sequence by In-Fusion cloning (TAKARA BIO, Shiga, Japan). This process is summarized in Figure S3 ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently stained using a horseradish peroxidase (HRP)-conjugated monoclonal anti-StrepTag antibody (Iba) or mouse anti-6His (Clontech) monoclonal antibody combined with rabbit anti-mouse-HRP polyclonal antibodies (DAKO) ...
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bioRxiv - Cell Biology 2023Quote: ... downstream of the Myh6 (α- myosin heavy chain) promoter and upstream of a human growth hormone polyadenylation signal using In-Fusion HD (Takara Bio cat# 011614). The final transgenic targeting construct was verified by DNA sequencing ...
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bioRxiv - Bioengineering 2019Quote: ... MO). The horseradish peroxidase (HRP)-conjugated monoclonal anti-His antibody (anti-mouse Cat. #631210) was obtained from Clontech (Mountain View, CA). The ultrasensitive HRP substrate used for Western blotting was from TaKaRa (Shiga ...
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bioRxiv - Plant Biology 2023Quote: ... Probes were detected with sensitive HRP substrates (Takara), and an iBrightTM CL750 Imaging System.
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bioRxiv - Synthetic Biology 2020Quote: ... dry milk and 0.1 % (v/v) Tween 20 and then incubated with mouse anti-6xHis tag monoclonal antibody-HRP conjugate (Clontech or Thermo Fisher Scientific, USA) in the same buffer for 2 h at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were stained with anti-mouse E-cadherin monoclonal antibody ECCD-2 (1:100, Takara) for 16 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... or Western BLoT Ultra Sensitive HRP Substrate (Takara Bio).
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bioRxiv - Molecular Biology 2020Quote: ... HRP-linked (1:2000, Takara, japan, Cat#T7122A-1);
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bioRxiv - Molecular Biology 2021Quote: ... HRP-linked (1:2000, Takara, Japan, Cat# T7122A-1).
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bioRxiv - Plant Biology 2022Quote: ... Bound proteins were detected using Hyper HRP Substrate (Takara). PtIns(3,5)P2 Grip protein (P-3516-3-EC ...
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bioRxiv - Cancer Biology 2023Quote: ... and developed using Western BLoT Chemiluminescent HRP Substrate (Takara). The chemiluminescent images were observed using FUSION FX (Vilber ...
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bioRxiv - Microbiology 2021Quote: ... or Western BLoT Ultra Sensitive HRP Substrate (Takara, Cat# T7104A) according to the manufacturers’ instruction ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated for 1 h at room temperature with Odyssey blocking buffer and 0.2 % PBS-T (PBS containing 0.2 % (v/v) Tween-20) with rat anti-HA clone 3F primary antibody (Clontech) at 1:1,000 dilution ...
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bioRxiv - Physiology 2022Quote: ... The insulin-like growth factor 1 (IGF1) coding sequence was PCR amplified (CloneAmp, Takara Bio; Cat. No. 639298) from cDNA generated from a rat gastrocnemius muscle ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (TaKaRa) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and as a prey to the activation domain of the GAL4 transcription factor (GAL4-AD) in plasmid pACT2 (Clontech). To monitor the induction of a HIS3 reporter gene by complexes of bait and prey fusion proteins ...
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bioRxiv - Biochemistry 2024Quote: ... The sequence of human histone H2A(K15C-129) was cloned into the pCold-Trigger factor (TF) vector (Takara Bio) with a SUMO coding sequence inserted to generate His6–TF–SUMO–H2A(K15C-129 ...
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bioRxiv - Immunology 2021Quote: ... immunoreactive bands were visualized using Western BLoT Quant HRP Substrate (Takara Bio) or Western BLoT Ultra Sensitive HRP Substrate (Takara Bio).