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Citations for Takara Bio :
1 - 50 of 366 citations for Transmembrane Serine Protease 11A TMPRSS11A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and K287A full-length aqMutLs were constructed by introducing the mutations into the pET-11a/aqmutL plasmid (47) using the PrimeSTAR mutagenesis procedure (Takara). The primer sets used for the construction of these mutant aqMutLs were 5’-TCGTAGAGGAACTCGTTGAGAATTCT-3’ and 5’-CGAGTTCCTCTACGACGTCAACAGGA-3’ ...
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bioRxiv - Microbiology 2020Quote: ... ramulus DSM 15684 were cloned without signal peptide or transmembrane domain from genomic DNA using In-Fusion cloning (Takara) and the primers in Table S8 into the EcoRI and Ncol restriction sites of the corresponding plasmids ...
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bioRxiv - Microbiology 2023Quote: ... Fragments encoding the CD45 extracellular domain (ECD) and transmembrane region were amplified by PCR and cloned into pEGFP-N1 (Clontech) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: The SYP121 DNA (obtained from Riken) was amplified from cDNA with primers excluding the transmembrane domain and cloned into the pGADT7 vector (Clontech Laboratories, Inc.). All other exocyst constructs used in the study have been previously described [38,48,73](Hála et al. ...
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bioRxiv - Cell Biology 2021Quote: Mouse IGFBP3 cDNA fused with a C-terminal 3xFlag (linked with a glycine-glycine-glycine-glycine-serine segment) was cloned into pQXCIP (Clontech) retroviral vector and sequence-confirmed ...
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bioRxiv - Neuroscience 2023Quote: ... HRV3C protease (TAKARA) was added overnight at 4°C and Ni-NTA was added to eliminate the cleaved portion overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... protease inhibitor (Takara, Japan), primary antibody (Cell signaling technology ...
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bioRxiv - Neuroscience 2023Quote: ... and protease inhibitors (Takara Bio, Kusatsu, Japan) mixes ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X protease inhibitor cocktail (635673; Takara Clontech), 1 mM PMSF ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X protease inhibitor cocktail (635673; Takara Clontech), 1 mM PMSF ...
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bioRxiv - Neuroscience 2020Quote: ... containing 1% protease inhibitor (Clontech, Mountain View, CA) and 1% phosphatase inhibitors (Millipore Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... containing 1% protease inhibitor (Clontech, Mountain View, CA) and 1% phosphatase inhibitors (Millipore Sigma) ...
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bioRxiv - Plant Biology 2023Quote: ... 0.05% T-20 and protease inhibitor cocktail (Takara)] ...
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bioRxiv - Microbiology 2021Quote: ... 10 U ml−1 of HRV- 3C protease (Takara) were added to the Ni-NTA eluted protein to remove the oligohistidine- GST-tag during dialysis against 3 L of dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... GST tags were cleaved by HRV-3C Protease (Takara), which released the recombinant proteins from the beads ...
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bioRxiv - Biochemistry 2023Quote: ... Cleavage of the His-tag by HRV3C protease (Takara, 7360) followed the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and 10% glycerol with complete protease inhibitor cocktail (Clontech Laboratories). Cells were incubated on ice for 20 minutes before centrifugation at 14,000 rpm at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... S-6P-NanoLuc proteins were released after HRV 3C protease (TaKaRa) treatment overnight at 4°C.
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bioRxiv - Molecular Biology 2022Quote: ... Lysozyme solution and Protease Inhibitor Cocktail (Takara Bio USA, Inc., CA). The suspension was sonicated 10 sec for 3 times with a 30 s pause ...
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bioRxiv - Microbiology 2020Quote: ... Purification tags were removed by treating recombinant proteins with HRV3C protease (TaKaRa) and cOmplete™ His-tag Purification Resin (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... and pH7.2) supplemented with the ProteoGuard EDTA-free protease inhibitor cocktail (Clontech, 635673). The cells in the suspended solution were sonicated using the Q125 sonicator (Qsonica ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining HRV-3C Protease was removed by TALON Metal Affinity Resin (Takara). In the rescue assays ...
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bioRxiv - Cancer Biology 2020Quote: ... The primary antibody rabbit anti-human Cas9 Polyclonal Antibody (Clontech) was diluted 1 ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal antibody (Clontech) and peroxidase-labeled anti-mouse IgG (H + L ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tagged TEV protease was removed by binding to Co2+-charged TALON resin (Clontech) and the flow-through concentrated using a 100 kDa molecular weight cut off concentrator (Corning) ...
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bioRxiv - Microbiology 2023Quote: ... anti-GAPDH antibody (Clontech) at a 1/2500 dilution ...
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bioRxiv - Genetics 2019Quote: ... Antibodies used were GAL4 AD Mouse Monoclonal Antibody (Takara Bio USA, Inc. #630402), GAL4 DNA-BD Mouse Monoclonal Antibody (Takara Bio USA ...
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bioRxiv - Neuroscience 2020Quote: ... and incubation with the primary antibody (polyclonal rabbit anti-dsRed antibody, 632496, Clontech, dilution 1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... 0.5% CHAPS supplemented with protease inhibitor cocktail) were first applied to a His60 Ni Superflow column (Clontech). Eluates from the His60 Ni column were pooled and dialyzed overnight against 50 mM sodium phosphate pH 7.4 and then loaded onto a DEAE column ...
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bioRxiv - Microbiology 2019Quote: ... The NusA tag of GfsB was cleaved with a HRV 3C protease at 4°C (Takara Bio) and removed by Ni-agarose ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HepG2 cells were resuspended in ice-cold PBS supplemented with protease inhibitors (Clontech, Mountain View, CA, USA) and lysed in ice bath by sonication in cycles of 10 s on /5 s off for 1 min at 6–10 μm amplitude at 25 % intensity from an exponential ultrasonic horn of 3 mm in a Soniprep 150 MSE (MSE Ltd. ...
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bioRxiv - Cell Biology 2019Quote: ... SMAD2 #3122 and SMAD3 #9523 antibodies (Cell Signalling Technology) and anti-FLAG antibody (Clontech). Horseradish peroxidase-conjugated secondary antibodies and anti-rabbit and anti-mouse antibodies were acquired from Molecular Probes ...
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bioRxiv - Synthetic Biology 2021Quote: ... The following antibodies were used: Anti-GFP antibody (JL-8 from Takara, Shiga, Japan), anti-Flag antibody (M2 from Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... or anti-GFP antibody (Clontech) for 1 hour at room temperature followed by a second incubation with HRP-conjugated secondary antibody (Santa Cruz Biotechnology) ...
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bioRxiv - Microbiology 2024Quote: ... Monoclonal Antibody (JL-8) (Clontech). Anti-mouse IgG was used as a secondary antibody.
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bioRxiv - Microbiology 2022Quote: ... 2X ProteoGuard EDTA-free protease inhibitor cocktail (catalog no. 635673, Takara Bio USA, Inc., San Jose, CA, USA), and DNase and RNase-free RPI molecular grade water (catalog no ...
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bioRxiv - Molecular Biology 2023Quote: ... purified MBP-mEGFP/mCherry-Seb1 and MBP-mEGFP/mCherry-Rhn1 proteins were incubated with HRV 3C protease (Takara; 1 U/1 g of target recombinant proteins ...
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bioRxiv - Cell Biology 2021Quote: ... 1982) or a commercial antibody against dsRed that detects mCherry (Living Colors DsRed Polyclonal Antibody, Clontech). After 3 consecutive 5-min washes in PBS ...
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bioRxiv - Plant Biology 2019Quote: Primary antibodies were monoclonal antibodies from mouse: α-GFP (JL-8, 1:5000, Takara, Shiga, Japan) or rat ...
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bioRxiv - Neuroscience 2023Quote: ... a mix of an mCherry-DsRed rabbit polyclonal antibody (Living Colors-Clontech Antibody 632496, 1:1000) and a mouse monoclonal antibody against oxytocin (P38 ...
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bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing N-terminal GST tag and TEV protease recognition sequence was inserted into pCold I (Takara), followed by the insertion of a DNA fragment containing C-terminal 2×HA tag by NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... N-terminal Flag-tagged P38α containing 3C protease site was amplified by PCR using CloneAmp HiFi PCR Premix (Takara) and ligated in to pcDNA3 using the aforementioned restrictions sites ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell pellets were resuspended in the prepared cell lysis buffer (0.1% Triton-X, 20 mM Tris, 100 mM NaCl) and ProteoGuard EDTA-Free protease inhibitor cocktail (Takara) for 30 minutes followed by sonication in ice cold water for 40 minutes (75 amplitude ...
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bioRxiv - Biochemistry 2021Quote: ... After incubation for 2 hours at 4°C the resin was washed extensively with buffer B and the flWT-Kv3.1 tetramer was eluted by addition of 100 µl of HRV 3C Protease at 1U/µl concentration from Takara Bio ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... combined with TaqStart Antibody (639250, Takara Bio Europe ...
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bioRxiv - Microbiology 2019Quote: ... Rabbit anti-GFP antibody (Clontech #632377) was used at 1:5,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: α-His primary antibody (1/10,000; Clontech) and HRP-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Molecular Biology 2022Quote: ... an anti-mCherry antibody (#Z2496N, TaKaRa), or a horseradish peroxidase-conjugated anti-α-tubulin antibody (#HRP-66031 ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.22 µg TaqStart Antibody (Clontech). 45-50 cycles of amplification were performed on a Bio-Rad C1000 thermocycler and the resulting product visualized with ethidium bromide on a 2% agarose gel.
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bioRxiv - Plant Biology 2022Quote: ... The anti-GFP antibody (TaKaRa, 632381), the anti-LhcB2 antibody (Agrisera ...