Labshake search
Citations for Takara Bio :
1 - 38 of 38 citations for TM 25659 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... TM-ABD or TM-ABD* fragments were then seamlessly cloned (Takara bio, San Jose, CA) into the linearized HaloTag vector by PCR amplification (primers details in Table S1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... while TaKaRa EpiTaq(tm) HS (Takara) was used for generic primers ...
-
bioRxiv - Immunology 2021Quote: ... using One Step TB Green(tm) PrimeScript(tm) RT-PCR Kit II (SYBR Green) (RR086B, TaKaRa, JAPAN). Relative copy number was determined by calculating the fold-change difference in the gene of interest relative to GAPTH ...
-
bioRxiv - Cancer Biology 2020Quote: ... using SYBR® Premix Ex Taq(tm) (Takara). Expression values relative to reference genes cyclophilin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
-
bioRxiv - Neuroscience 2020Quote: ... with SYBR Premix Ex Taq TM II (Takara Bio). The specificity of amplicons was examined by melting curve analysis and agarose gel electrophoresis ...
-
bioRxiv - Neuroscience 2023Quote: ... by using SYBR Premix Ex Taq TM (TAKARA, Japan) and parameters were ...
-
bioRxiv - Plant Biology 2024Quote: ... with the SYBER® Premix Ex Taq TM II kit (TaKaRa) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... Transformations have been performed using Stellar(tm) Competent Cells (Takara, Cat#636763).
-
bioRxiv - Molecular Biology 2020Quote: ... VAL2 and VAL3 were amplified by PCR (PrimeSTAR TM HS DNA Polymerase, TaKaRa) using the plasmids carrying the full-length cDNA of each gene as template ...
-
bioRxiv - Immunology 2020Quote: ... qRT-PCR was performed using TB Green TM Premix Ex TaqTM II (Takara). The following was the reaction system ...
-
bioRxiv - Cell Biology 2019Quote: ... The SYBR® Premix Ex Taq TM II kit (Takara, Beijing, China, RR82LR) reagent was used to detect the expression of miR195 by real-time quantitative PCR using the Applied Biosystems 7500 Real-Time PCR System (Thermo ...
-
bioRxiv - Molecular Biology 2020Quote: ... Adenoviruses were produced and amplified in Adeno-X(tm) 293 cells (Takara, # 632271), while titter was determined by fluorescence-activated cell sorting.
-
bioRxiv - Plant Biology 2022Quote: Y2H was performed using the Matchmaker TM GAL4-based two-hybrid system (Clontech) as described in Schrumpfová et al ...
-
bioRxiv - Cancer Biology 2024Quote: ... HA-RNF43-ECD-TM were constructed by subcloning using In-Fusion (TaKaRa, 639642). Plasmids were further constructed for lentiviral expression by subcloning into pBobi vector by In-Fusion.
-
bioRxiv - Cancer Biology 2020Quote: ... and reverse-transcribed into cDNA using the PrimeScript(tm) RT Reagent Kit (RR047A, TaKaRa, Japan). Quantitative real-time PCR analysis of target mRNA-expression levels was achieved using SYBR Green qPCR Master Mix (Bio-Rad ...
-
bioRxiv - Immunology 2020Quote: ... 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio ...
-
bioRxiv - Developmental Biology 2022Quote: ... 0.6 μl of reaction mix (16.7 U/μl SMARTScribe TM Reverse Transcriptase (Takara Bio, 639538), 1.67 U/μl Recombinant RNase Inhibitor (Takara Bio ...
-
bioRxiv - Plant Biology 2022Quote: ... Genomic DNA removal and reverse transcription were performed with PrimeScript TM RT regent kit (Takara). qPCR reaction was performed using iTaq Universal SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Molecular Biology 2020Quote: ... The qRT-PCR analyses were performed using SYBR Premix ExTaq TM II Mix (TaKaRa, Shiga, Japan). Actin3 (GenBank accession no. ...
-
bioRxiv - Neuroscience 2022Quote: ... PolyA+ mRNA was extracted from total RNA using Oligotex TM-dT30 mRNA Purification Kit (Takara Bio), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... RT–PCR was performed using a TB Green TM Premix Ex Taq Kit (Cat# RR820A, Takara) on a Light Cycler Real-Time PCR System (480II ...
-
bioRxiv - Immunology 2020Quote: ... The reverse transcription of RNA into cDNA was performed using a Prime Script TM RT Master Mix (Takara). The following was the reaction system ...
-
bioRxiv - Cell Biology 2021Quote: ... cDNA products were amplified by qPCR using the SYBR®Premix Ex Taq TM kit (Takara, RR420A, Japan) in an Applied Biosystem 7500 Fast Real-Time PCR System under standard PCR conditions ...
-
bioRxiv - Microbiology 2022Quote: ... Viral load was measured by RT-qPCR using One-Step SYBR® Primescript(tm) RT-PCR kit II (Takara). CT values from serum samples were used to calculate serum viral load according to regression equation built by a set of standard viral RNA extracted from dilutions of known titre virus preparation ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... then cDNA was synthesized according to the PrimeScript TM II 1st Strand cDNA Synthesis Kit (Takara Bio, Dalian, China). Real-time PCR analysis was performed with the LightCycler 480 SYBR Green I Master (Roche ...
-
bioRxiv - Cell Biology 2024Quote: ... 1×105 cells of HeLa cells or BJ-5ta cells expressing inducible TM-mNG21-10 were transfected with 7.5 pmol of Cas9 (TAKARA), 7.5 pmol of sgRNA ...
-
bioRxiv - Genetics 2019Quote: ... Isolated RNA was reverse transcribed into cDNA was by Prime Script TM RT reagent Kit with gDNA Eraser (Stratagene, Takara). Real-time PCR was performed on ABI-7500 real-time PCR machine (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2019Quote: ... NEK9 lentiviral particles for each construct were harvested from transfected 293T packaging cells using the Lenti-X(tm) HTX Packaging System (Clontech), transduced into U2OS parental cells and selected in fresh media supplemented with 2 mg/ml of G418 and 1 µg/ml puromycin over several days ...
-
bioRxiv - Cell Biology 2020Quote: ... Mouse E-Syt3 cDNA was amplified by PCR from a cDNA library constructed with poly(A) RNA from 3T3-L1 adipocytes (Mate & Plate TM Library System, Clontech) and cloned into the PT-Adv vector using 5’and 3’ E-Syt3 primers (Table 1) ...
-
bioRxiv - Neuroscience 2020Quote: ... the RNA sample was diluted into 140 ng/μL and purified with the Primescript TM RT regent kit (Takara, RR047). cDNA was generated using SYBR® Premix Ex Taq™ II kit (Takara ...
-
bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR analysis was performed on an Agilent Real-Time qPCR apparatus (Mx3000P system) using SYBR Premix ExTaq TM II (Takara, Japan). Biological replicates for each set of experiments were carried out three times ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Bcl-2 and PTEN were detected by RT-PCR with TB Green TM Premix Ex TaqTM II(Tli RNaseH Plus) (RR820A, Takara, Japan). The complete gene sequences showed in Table 2 were searched from the National Center for Biotechnology Information (NCBI ...
-
bioRxiv - Plant Biology 2023Quote: ... which was then reverse-transcribed into first-strand cDNA using the Prime-Script-TM RT Reagent Kit (RR036, Takara, Kyoto, Japan). The quantitative real-time polymerase chain reaction was performed using the ABI 7300 sequencer and SYBR Premix Ex Taq-TM (RR420 ...
-
bioRxiv - Microbiology 2024Quote: ... reactions were performed to insert these linear fragments into the pMV306 previously digested with EcoRV and transformed into Escherichia coli (E. coli) Stellar TM competent cells (Takara Bio), purified (NucleoSpin Plasmid ...
-
bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was carried out to evaluate the syndecan-4 expression between lenti-synd4 shRNA and lenti-null group with SYBP Premix Ex Taq TM from Takara (Shiga, Japan) following the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Spec.100) and TB Green TM Premix Ex TaqTM II (Tli RNaseH Plus) (RR820A, Spec. 200) were supplied by Takara (Beijing, China). IP cell lysate (P0013 ...
-
bioRxiv - Plant Biology 2023Quote: ... The quantitative real-time polymerase chain reaction was performed using the ABI 7300 sequencer and SYBR Premix Ex Taq-TM (RR420, Takara, Kyoto, Japan) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2019Quote: ... This region was PCR amplified with DinoSL and KbrSRP-U6R1 primer set (sequences and Tm in Table 2) using the high fidelity PrimeSTAR HS DNA Polymerase (Takara, Kusatsu, Shiga Prefecture, Japan) at 94°C for 1 min ...