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Citations for Takara Bio :
1 - 50 of 594 citations for Serpin G1 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: HEK-293 Tet-Off (HEK-293TO) cells (Clontech) and HEK-293 Flp-In T-REx cells (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: HEK GP 293 cells (Clontech) were transfected with plasmid DNA using TurboFect Transfection reagent (ThermoFisher scientific ...
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bioRxiv - Biophysics 2022Quote: ... LX-293 HEK cells (Clontech) were co-transfected with LentiCRISPRv1 or LentiCRISPRv2 ...
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bioRxiv - Synthetic Biology 2023Quote: Human Embryonic Kidney (HEK) 293 cells (ATCC, CRL-1573) and HEK293T-LentiX (Takara Biosciences) were cultured in standard culture conditions (37ºC and 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: ... HEK-293 T cells were transfected with calcium phosphate (CalPhos, Clontech) in T75 flasks using 30 μg of plasmid DNA ...
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bioRxiv - Cell Biology 2019Quote: HEK 293 Tet-On® 3G cells were purchased from Clontech and cultured in Minimum Essential Medium (MEM ...
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bioRxiv - Synthetic Biology 2024Quote: WT Human Embryonic Kidney (HEK) 293 cells (ATCC, catalog no. CRL-1573) and HEK293T-LentiX (Takara Biosciences, catalog no. 632180) were cultured in a humidity-controlled incubator under standard culture conditions (37 °C with 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... Retroviral production was performed by transient transfection of HEK-GP2 293 cells (Clontech) with 0.8 μg pCMVsVg and 1.2 μg of pLPC-puro-N-Flag-NELFE plasmids using JetPrime reagent (polyplus) ...
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bioRxiv - Immunology 2022Quote: ... Viruses were packaged in human embryonic kidney (HEK) 293 cells and viral supernatants were processed using Retro-X concentrators (Takara Bio. USA Inc). Naïve CD8 T cells were purified by negative-selection and stimulated in vitro with plate-bound anti- CD3/CD28 ...
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bioRxiv - Biochemistry 2021Quote: ... Human embryonic kidney EcoPack 2–293 cells (Clontech) were cultivated on collagen-coated (Collagen R ...
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bioRxiv - Cell Biology 2020Quote: Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
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bioRxiv - Cell Biology 2021Quote: Human embryonic kidney 293 T cells (HEK293T, Takara, Cat# 632180) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Immunology 2024Quote: ... HEK 293T cells were sourced from the American Type Culture Collection (ATCC) and GP2-293 from Takara Bio ...
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bioRxiv - Cell Biology 2024Quote: ... Human embryonic kidney (HEK) cell lines (Lenti-X 293T) (Takara) were cultured in high-glucose DMEM (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... we used jetPrime (Polyplus) according to the manufacturer’s instructions to transfect Lenti-X 293 T HEK cells (Takara) with lentiviral production plasmids pMD2.G and psPAX2 along with our individual transfer plasmids ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293/17 cell media were harvested and concentrated overnight with a Lenti-X concentrator (Clontech, Mountain View, CA) according to the manufacturer’s protocol and resuspended in 10-fold less media volume prior to concentration.
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bioRxiv - Biochemistry 2021Quote: ... Purification procedure was similar to that for HEK 293 cells except for the usage of cobalt-based affinity resin (Talon, Clontech) to capture His-tagged proteins.
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bioRxiv - Biochemistry 2020Quote: Induced expression of the bidirectional plasmid required the use of HEK 293 Tet-On® 3G cells (Takara Clontech Cat# 631182). Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008 ...
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bioRxiv - Biochemistry 2020Quote: Induced expression of the bidirectional plasmid required the use of HEK 293 Tet-On® 3G cells (Takara Clontech Cat# 631182). Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008 ...
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bioRxiv - Biochemistry 2023Quote: ... Human embryonic kidney (HEK) 293T Lenti-X were obtained from Takara (cat# 632180). These cells transformed with adenovirus type 5 DNA and expresses the SV40 large T antigen ...
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bioRxiv - Immunology 2020Quote: Transferrin over-expression or knockdown vectors were constructed and HEK 293T cells (Conservation Genetics CAS Kunming Cell Bank, China) and EcoPack™ 2–293 cells (Clontech, USA) were used to package lentiviruses and retroviruses ...
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bioRxiv - Molecular Biology 2024Quote: The human embryonic kidney (HEK) 293T cell line was purchased from Takara (Cat# 632180) and cultured in a humidified 95% air / 5% CO2 incubator at 37°C in a standard Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequences derived from pRetroX-G1-Red and pRetroX-SG2M (Clontech) were cloned into pHR lentiviral vectors under puromycin or blasticidin selection ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were collected at 24 and 48 h after transfection of HEK 293/17 cells and concentrated using a LentiX concentrator (Takara Bio USA, Inc., Mountain View, CA). HEK 293/17 cells or NSC cells were plated in 6 well plates at a density of 105 per well ...
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bioRxiv - Cell Biology 2021Quote: ... 293-LVX cells (Clontech) were transfected with pMSCV and pCl-Eco plasmids using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: HEK 293T cells (Clontech) and its derivative ...
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bioRxiv - Cell Biology 2020Quote: Lenti-X 293 T (Clontech), Neuro2A (mouse neuroblastoma ...
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bioRxiv - Cancer Biology 2020Quote: ... into GP2-293 cells (Clontech). Virus was harvested and cleared via centrifugation at 48 hours post-transfection and infections were carried out in the presence of 10 μg/mL of polybrene (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... EcoPack 293 cells (Takara Bio) were plated in 6-well plates (3 x 105/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Individual lentiCRISPRv2 vectors were introduced along with packaging vectors into human embryonic kidney (HEK) 293T cells via calcium phosphate transfection according to the manufacturer’s protocol (Clontech). Lentivirus was harvested at 48 and 72 hours after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters prior to transduction of cancer cell lines using centrifugation at 1000 g for 2 hours in the presence of 8 μg/mL of polybrene (Santa Cruz Biotechnology) ...
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bioRxiv - Molecular Biology 2023Quote: ... His (Clontech), supplemented with 1.5 mM 3-amino-1,2,4-triazol (3-AT ...
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bioRxiv - Synthetic Biology 2023Quote: ... LentiX HEK 293T cells (Clontech) were cultured in DMEM + GlutaMAX (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: Lenti-X HEK-293Ts (Takara) were used for lentivirus production and maintained at below 80% confluency in DMEM supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2020Quote: We transiently co-transfected cDNA constructs of GluN1 and GluN2A into mammalian human embryonic kidney 293 (HEK293) with a separate pEGFP-Cl vector (Clontech) at a ratio of 4.5:4.5:1 (GluN1/GluN2A/EGFP ...
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bioRxiv - Cancer Biology 2020Quote: ... retroviral Packaging Cells (GP2-293; Clontech) were transfected with pVSV-G (Clontech ...
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bioRxiv - Bioengineering 2023Quote: ... GP2-293 cell line (Takara, CA) was used as the viral packing cell line for plasmid encoding CAR construct ...
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bioRxiv - Microbiology 2023Quote: ... or TransIT-293 (Takara, Cat# MIR2700) with Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... using TransIT®-293 reagent (Takara). Two days later ...
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bioRxiv - Cancer Biology 2023Quote: ... into GP2-293 cells (Takara 631458). Lentivirus was generated by transfecting the PIKFYVE-containing plasmid with the packaging plasmids pMD2.G and psPAX2 (Addgene 12259 and 12260 ...
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bioRxiv - Neuroscience 2020Quote: ... Lenti-X HEK 293T cells (ClonTech) were used for producing lentiviral particles ...
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bioRxiv - Neuroscience 2024Quote: HEK-293T (Takara cat. no. 632617), HEK293-EGFP (GenTarget ...
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bioRxiv - Developmental Biology 2021Quote: ... –His (630428, Clontech) or –Trp ...
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bioRxiv - Developmental Biology 2021Quote: ... –His (630419, Clontech) selective media +3 mM ...
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bioRxiv - Plant Biology 2022Quote: ... and His (Clontech) with and without the addition of 3-Amino-1H-1,2,4-triazole (Acros Organics (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... Purification of His-tagged proteins was carried out using His-tag affinity resin (His-resin) (Clontech, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... Lenti-X 293 T cells (Takara Bio) grown in 10 cm dish were transiently transfected with the following plasmids ...
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bioRxiv - Immunology 2022Quote: 5 × 106 GP2-293 packaging cells (Clontech) were plated in a 10 cm dish containing D10 medium (DMEM supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2020Quote: Gp2-293 packaging cell line (Clontech #631458) was grown in DMEM (Sigma-Aldrich #D5796 ...
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bioRxiv - Cell Biology 2022Quote: ... GP2-293 cells were purchased from Takara Bio USA (#631458) ...
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bioRxiv - Molecular Biology 2023Quote: ... Lenti-X 293 cells (Takara Bio Inc.) were seeded and transfected with pSIH-H1 shRNA lentivectors (System Biosciences ...