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Citations for Takara Bio :
1 - 50 of 575 citations for SARS Coronavirus Envelope Protein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... reactions were performed to insert these linear fragments into the pMV306 previously digested with EcoRV and transformed into Escherichia coli (E. coli) Stellar TM competent cells (Takara Bio), purified (NucleoSpin Plasmid ...
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bioRxiv - Systems Biology 2023Quote: ... Ligated plasmid products were transformed into stellar competent cells (E. coli HST08 strain, Takara Bio). See Table S1 for full descriptions of constructs.
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bioRxiv - Immunology 2023Quote: ... RVFV L segment RNA and SARS E RNA were detected with PrimeDirect™ Probe RT-qPCR Mix (Takara, RR650A) according to manufacturer’s instructions using RVFV L primers fwd 5’ TGAAAATTCCTGAGACACATGG 3’ ...
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bioRxiv - Systems Biology 2023Quote: ... was used as the wild-type (WT) strain for genetic manipulations. Chemically competent Escherichia coli (E. coli) DH5α and HST08 (Stellar Competent Cells, TaKaRa Bio Inc., Kusatsu, Shiga, Japan) were used as the host strains for molecular cloning.
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bioRxiv - Immunology 2021Quote: ... envelope vector into Lenti-X 293T cells (Clontech) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The SARS-CoV-2 viral load was quantified in culture supernatant using RT-QPCR with primer probes targeting E gene of SARS-CoV-2 using PrimeDirect Probe RT-qPCR Mix (TaKaRa Bio USA, Inc) and Applied Biosystems QuantStudio3 real-time PCR system (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... coli HST08 (used to test coregulation of genes in newly-formed operons and for vector construction; E. coli HST08 Premium Competent Cells, Takara Bio, Japan, 9128).
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bioRxiv - Microbiology 2020Quote: ... coli (Clontech), extracted and retransformed into EAW19 ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified from Escherichia coli BL21 (DE3) with TALON Metal Affinity Resin (Takara), Pierce Glutathione Agarose (Thermo scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant protein was produced in Escherichia coli BL21 (DE3) and purified with TALON resin (Clontech). For immunization ...
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bioRxiv - Microbiology 2021Quote: 293T were transfected with full length SARS-CoV-2 Spikes and a green fluorescent protein (GFP) expressor (pIRES2-eGFP; Clontech) using the calcium-phosphate method ...
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bioRxiv - Pathology 2021Quote: ... Expression of the GST-N protein of SARS-CoV-2 was induced by isopropyl-D-1-thiogalactopyranoside (0.3 mM IPTG, Takara Bio). The cell pellets were sonicated ...
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bioRxiv - Microbiology 2022Quote: ... and E484Q mutations of the SARS-CoV-2 spike protein were determined by qPCR using mutation detection kits purchased from Takara Bio (Shiga ...
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bioRxiv - Microbiology 2019Quote: ... coli cells (Takara) and selected on LB-Ampicillin plates ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (TaKaRa) were transformed with these plasmids for amplification and DNA storage ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5α (Takara) or Stellar™ (Takara ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Clontech 636763) via heat shock ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coli (Takara,#9027) were transformed using electroporation and the subcloned library was obtained by combining 4 maxipreps ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5α (Takara) and Stellar™ (Takara) ...
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bioRxiv - Microbiology 2022Quote: ... coli Stellar (Takara) and E ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Takara Bio). Plasmids were purified by miniprep (Qiagen ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli Stellar (Takara) cells on LB- or M9 minimal-agar supplemented with 1 mM IPTG were inoculated into 250 μL of the same liquid medium and grown in a 1.5-mL 96-deep well plate at 240 rpm for ~10 generations ...
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bioRxiv - Genomics 2023Quote: ... coli (Takara Bioscience). 1% of the transformed cells were plated on ampicillin-agar plates to ensure adequate transformation efficiency (at least 10-fold library coverage) ...
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bioRxiv - Genomics 2023Quote: ... coli (Takara, 636763) and 10 colonies were picked at random to ensure that each colony was unique ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were expressed in Escherichia coli strain BL21(DE3) cells containing chaperone plasmid pG-KJE8 (TAKARA, 3340). When the optical density at 600 nm reached 0.6 ...
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bioRxiv - Immunology 2022Quote: ... GP2-293 retroviral packaging cells and the p10A1 envelope vector were purchased from Clontech. Lipofectamine 3000 was purchased from ThermoFisher and Polybrene was purchased from Sigma ...
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bioRxiv - Immunology 2021Quote: pVAX1-SARS-CoV2-co was designed by Takara, which encoded a highly optimized DNA sequence encoding the SARS-CoV-2 Spike glycoprotein ...
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bioRxiv - Physiology 2020Quote: ... coli Stellar cells (Clontech/Takara Bio USA Inc. ...
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bioRxiv - Bioengineering 2019Quote: ... coli BL21 (DE3) (TaKaRa, hsdS ...
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bioRxiv - Biophysics 2020Quote: ... coli (TOYOBO or TaKaRa), calf intestine (Roche) ...
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bioRxiv - Bioengineering 2019Quote: ... coli cells (Stellar, TaKaRa) were used for all cloning steps and were grown in LB medium supplemented with antibiotics as required - ampicillin (100 μg/mL) ...
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bioRxiv - Microbiology 2021Quote: ... coli Stellar™ (Takara) following standard protocols [56] ...
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bioRxiv - Microbiology 2019Quote: ... coli strain StellarTM (ClonTech), prior to isolation and transformation into COH1 GBS ...
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bioRxiv - Microbiology 2019Quote: ... coli cells (Stellar, TaKaRa), grown in LB medium at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... coli cells (Stellar, TaKaRa). A synthetic version of the E ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (Takara Bio) and positive clones were identified via RFP selection before sequencing to confirm the cloning was successful.
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bioRxiv - Microbiology 2022Quote: ... coli Stellar cells (Takara) for non-R6K origin of replication-based vectors or PIR2 cells (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... coli HST08 (Takara Bio). The pLI50_pgl plasmid was then isolated and transformed by electroporation into the restriction-deficient strain RN4220 ...
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bioRxiv - Genomics 2022Quote: ... coli cells (Takara Bio) and plasmid was prepared following standard protocols ...
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bioRxiv - Microbiology 2022Quote: ... coli Stellar (Takara Bio) cells and plated on selective Luria-Bertani (LB ...
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bioRxiv - Microbiology 2023Quote: ... coli StellarTM (Takara Biosciences) was used for cloning and plasmid storage ...
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bioRxiv - Developmental Biology 2023Quote: ... coli (Takara Bio, 636763) via ampicillin-resistant selection ...
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bioRxiv - Genomics 2023Quote: ... coli cells (Takara Bioscience). Sanger sequencing (Genewiz ...
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bioRxiv - Developmental Biology 2023Quote: ... coli (Takara Bio, 636763). DNA was extracted by miniprep (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... coli Stellar cells (Clontech) and plated onto Luria Bertani agar supplemented with 25 µg/ml chloramphenicol ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21 (DE3, Takara).
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bioRxiv - Bioengineering 2024Quote: ... coli DH5α (Takara Bio) was used for molecular cloning and cultivated in Luria-Bertani (LB ...
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bioRxiv - Neuroscience 2022Quote: ... and VSV-G (viral envelope) (Stewart et al., 2003) into Lenti-X HEK293T cells (Clontech) at a mass ratio of 5:4:3 using polyethyleneimine (PEI) ...
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bioRxiv - Pathology 2021Quote: ... The number of SARS-COV-2 copies were quantified using Direct One-Step RT-qPCR Mix for SARS-CoV-2 kit (Takara Bio Inc.).
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bioRxiv - Developmental Biology 2022Quote: ... rat anti-E-Cadherin (TAKARA; M110), rat anti-Endomucin (Santa Cruz ...