Labshake search
Citations for Takara Bio :
1 - 50 of 191 citations for Round Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Flow-sorted cells were cultured in ex vivo GT media in a 96 well flat bottom plate coated with 33.3 ug/ml of Retronectin (Takara). The 62 hr protocol consisted of pre-stimulation in GT media (24 hr ...
-
bioRxiv - Immunology 2019Quote: ... 96-well plates were coated with Retronectin (Clontech) overnight at 4°C ...
-
bioRxiv - Biochemistry 2019Quote: ... a 96-well Capturem His-tagged purification kit (Clontech) was used according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... into 96-well plates containing 2 µL Lysis Buffer (Clontech) supplemented with 1 U/μL RNase inhibitor (NEB) ...
-
bioRxiv - Bioengineering 2022Quote: ... the 96-well plate can be coated with RetroNectin® (Clontech/Takara ...
-
bioRxiv - Cancer Biology 2020Quote: ... Each well of the 96-well plate contained 3µL lysis buffer (10U Recombinant RNase Inhibitor (Takara Bio), 0.2% Triton X-100 (Sigma-Aldrich) ...
-
bioRxiv - Bioengineering 2022Quote: ... the 96-well plate can be coated with RetroNectin® (Clontech/Takara; 12µg/ml PBS according to manufacturer’s recommendations ...
-
bioRxiv - Bioengineering 2020Quote: ... followed by spinoculation in a 96-well plate coated with Retronectin (Takara, T100B). T cells were further expanded and dynabeads were removed prior to downstream procedures (e.g. ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The protein library was purified using a His-Tagged 96-well plate cartridge (Clontech) and buffer exchanged into 50 mM Tris-HCl ...
-
bioRxiv - Synthetic Biology 2019Quote: ... and the supernatants were transferred to a new 96-well plate with 50 μl Talon resin in each well (Takara Bio, Japan). Unspecific binding of proteins to the resin was reduced by adding imidazole to a final concentration of 10 mM in each well ...
-
bioRxiv - Neuroscience 2024Quote: ... and individual cells were captured in separate wells of a 96-well plate containing 4 μl lysis buffer (1 U/μl RNase inhibitor [Clontech, Cat#: 2313B]), 0.1% Triton (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2021Quote: ... Real-time qPCR was performed in optical 96-well plates using a TP950 (Takara, Japan). Fluorescence threshold data (Ct ...
-
bioRxiv - Cell Biology 2022Quote: cDNA was generated from single cells in the 96-well plate using the SmartSeq v4 kits (Takara Bio) using 1/4th volume reactions dispensed using a Mantis dispenser (Formulatrix) ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were single-cell sorted into 96-well low-bind PCR-plates [Eppendorf] containing 3 μl of lysis buffer (0.5 units/μl RNase inhibitor [Takara] ...
-
bioRxiv - Genetics 2020Quote: ... into a 96-well plate with qPCR buffer [SYBR Premis Ex TaqTM (Tli RHaseH Plus)] (RR420A; TAKARA, Tokyo, Japan) with 0.4 uM primers (Table S2 ...
-
bioRxiv - Immunology 2020Quote: ... non-tissue culture treated 96-well plates were coated overnight at 4 °C with 1 ml of retronectin (Takara) at 25μg/ml in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... single-cells were isolated using a BD FACS Aria III for sorting individual cells into 96-well plates prefilled with lysis buffer (0.26 μl of 10×Lysis buffer (Takara), 0.03 μl of RNase Inhibitor (100 U/μl ...
-
bioRxiv - Neuroscience 2020Quote: ... Single cells were sorted into 96-well plates containing 4uL lysis buffer containing 4U Recombinant RNase Inhibitor (Takara Bio 2313B), 0.05% Triton X-100 ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 × 104 SH-SY5Y cells in 90 µL of growth media containing no antibiotics were plated in opaque TC-treated 96-well plates containing Xfect (Clontech)/DNA transfection complexes ...
-
FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSSTbioRxiv - Plant Biology 2023Quote: ... Mating was carried out in a 96-well plate according to manufacturer’s instructions (Matchmaker GAL4 Two Hybrid System (Clontech/TakaraBio). Mated diploid strains were serially diluted and plated on - Leu–Trp double dropout (DDO ...
-
Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... BBQ and FICZ as indicated at 37°C in 96-well plates for 5 days prior to measuring viability using WST reagent (Takara) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... and GC B cells (live singlet CD19+ CD4− IgDlo CD71+CD38int CD20+ CXCR5+) were sorted using a FACSAria II into 96-well plates containing 2 μL Lysis Buffer (Clontech) supplemented with 1 U μl−1 RNase inhibitor (NEB) ...
-
bioRxiv - Immunology 2023Quote: ... into 96-well plates containing 12.5μl CDS sorting buffer as described in the SMART-Seq® HT Kit protocol (Takara Bio). Full-length cDNA amplification of single cells was performed per the kit protocol (Takara Bio) ...
-
bioRxiv - Neuroscience 2023Quote: ... we harvested 10-12 GFP+ cells per fly into 0.5ul nuclease free water in the pipette tip and then the tip was broken into a 96 well PCR tube containing RNAse inhibitors and buffer as described by Clontech’s ultra low HV SMARTer Ultra Low RNAseq kit (Catalog #634823) ...
-
bioRxiv - Bioengineering 2023Quote: ... The first-round PCR was performed using LA Taq DNA polymerase (Takara) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... freshly harvested single cells were sorted into 96-well plates containing the lysis buffer (0.2% triton-X-100, 1U/µl RNase inhibitor (Takara Bio, 2313A). Reverse transcription was performed using SuperScript II (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... Single cells were sorted by FACS as described above into 96-well plates containing 0.1 μl of RNase inhibitor (Clontech, Cat# 2313A), 1.9 μl of 0.2% Triton X-100 (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... We performed the second round of PCR using Premix Taq™ (TAKARA, RR902Q) in a 50μl reaction volume ...
-
bioRxiv - Cell Biology 2024Quote: ... Flanking att sequences were added through another round of PCR (Takara, cat # R011) and purified ...
-
bioRxiv - Neuroscience 2022Quote: ... single GFP+ cells were captured into 96-well plate with 11.5 μl lysis buffer and RNase Inhibitor without the CDS IIA Oligo (Takara Bio, Mountain View, CA), and stored at −80°C until used for construction of cDNA libraries ...
-
bioRxiv - Neuroscience 2023Quote: ... The qPCR reaction was performed on 96-well plates in a final volume of 12 μL using the Premix Ex Taq Probe based qPCR assay (Takara Biotechnology, ref. RR390A). All reactions were run in duplicate on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2020Quote: ... Reverse PCRs were performed in two rounds using CloneAmp HiFi PCR premix (Clontech, Mountain View, CA, USA). The first round was performed adding the forward and reverse primers in two independent reactions and consisted of 3 cycles of 98 °C for 10 seconds ...
-
bioRxiv - Molecular Biology 2021Quote: ... the gene encoding for eBFP1.2 was generated by multiple rounds of site-directed mutagenesis of pEGFP-C1 (Clontech). Next ...
-
bioRxiv - Genomics 2022Quote: ... prior to another round of PCR amplification for 16-18 cycles using either LA-Taq or PrimeSTAR GXL (Takara) at an annealing temperature of 60℃.
-
bioRxiv - Microbiology 2022Quote: ... Seven Ns were added into the primers as the barcode for barcoded-subamplicon sequencing during the first round PCR (PrimeSTAR Max DNA Polymerase, Takara Bio ...
-
bioRxiv - Immunology 2022Quote: ... T cells were infected with either replication-competent HIV-1 (pAD8gNef [80] or pNLgNef [79] or single-round HIV-1 (pHR-CD8a-d2eGFP-IRES-Nef) using Lenti-X Accelerator (TaKaRa) following the manufacturer’s protocol.
-
bioRxiv - Microbiology 2022Quote: ... the first round of PCR amplification was performed using Takara Ex Taq® Hot-Start Version (RR006A; TaKaRa Bio, Shiga, Japan). The amplification comprised 40 cycles of denaturation at 98 °C for 10 s ...
-
bioRxiv - Microbiology 2022Quote: ... The reverse transcription and the first round PCR were performed in a 25μl reaction volume by using PrimeScript™ One-Step RT-PCR Kit (TAKARA, RR055A). Cycling conditions were as follows ...
-
bioRxiv - Genetics 2020Quote: ... First-round reverse transcription PCR (RT-PCR) was conducted by using PrimeScript One Step RT-PCR Kit Ver.2 (TaKaRa, Dalian, China). A 10 μL reaction mixture contained 5 μL of 2 × 1 Step Buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were seeded in one well of a 6 well plate in supplemented DEF-CS (Takara). After a recovery period of 5 days ...
-
bioRxiv - Immunology 2021Quote: ... 24-well non-tissue culture plate wells were pre-coated with RetroNectin® (Takara Bio USA, Inc.) according to manufacturer’s instructions and then day 10 cultured Tregs were added at 0.3 x 106 cells/well in 0.3mL of cell culture media ...
-
bioRxiv - Cancer Biology 2020Quote: ... three replicate wells of 5□×□104 cells per well were seeded in a retronectin (Takara Bio Inc, JPY) coated 24-well XF24 plate ...
-
bioRxiv - Microbiology 2020Quote: ... as well as siRNA ladder marker (Takara), were separated on a 15% denaturing polyacrylamide gel electrophoresis (PAGE ...
-
bioRxiv - Biochemistry 2024Quote: ... a two-round PCR strategy was required to amplify the ORF using a high-fidelity PrimeStar ® DNA polymerase (Takara Europe, Saint-Germain-en-Laye, France). The first round of the nested PCR was carried out using the above cDNA as the template with primers designed in the 5’- and 3’-untranslated regions (UTR ...
-
bioRxiv - Immunology 2019Quote: ... Non-tissue culture treated 24-well plates were coated with 0.5 mL per well of 10 μg/mL RetroNectin (Takara) on day 1 and stored overnight at 4°C ...
-
bioRxiv - Genomics 2022Quote: ... we employed SMARTer RNA unique dual index kit -96 U (Takara Bio, 634452). SMARTer RNA unique dual index of each 5’ and 3’ PCR primer were added to each sample to distinguish pooled libraries from each other ...
-
bioRxiv - Genomics 2021Quote: The NucBlue and DAPI co-stained single-nuclei suspension (60□cells/µl) was distributed to eight wells of a 384-well source plate (Takara) and then dispensed into a barcoded SMARTer ICELL8 3’ DE Chip (Takara ...
-
bioRxiv - Developmental Biology 2022Quote: iPSCs SFCi55 and hESCs RUNX1-GFP were plated at 3 × 105 cells per a well of a 6 well plate and reverse transfected with 2 μg of DNA using the Xfect Transfection reagent (Clontech) and analyzed 2 days later.
-
bioRxiv - Cell Biology 2024Quote: iPSCs SFCi55 and hESCs RUNX1-GFP were plated at 3 × 105 cells per a well of a 6 well plate and reverse transfected with 2 μg of DNA using the Xfect Transfection reagent (Clontech) and analyzed 2 days later.
-
bioRxiv - Neuroscience 2020Quote: SH-SY5Y cells were seeded on the glass bottom plates and transfected with 0.5 μg of Mito dsRED plasmid (Clontech) using lipofectamine 2000 (Invitrogen) ...