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Citations for Takara Bio :
1 - 50 of 682 citations for Recombinant E. coli MUG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified from Escherichia coli BL21 (DE3) with TALON Metal Affinity Resin (Takara), Pierce Glutathione Agarose (Thermo scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant protein was produced in Escherichia coli BL21 (DE3) and purified with TALON resin (Clontech). For immunization ...
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bioRxiv - Developmental Biology 2021Quote: ... and recombinant Cas9 proteins (Clontech) were introduced together into the KhES-1 cells by electroporation (Neon device ...
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bioRxiv - Molecular Biology 2023Quote: ... The recombinant Cas9 protein (TAKARA) and the sgRNA were incubated for 10 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... reactions were performed to insert these linear fragments into the pMV306 previously digested with EcoRV and transformed into Escherichia coli (E. coli) Stellar TM competent cells (Takara Bio), purified (NucleoSpin Plasmid ...
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bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were transformed into Escherichia coli BL21 (Takara Bio Co Ltd, Japan) and the transformed cells were cultured at 37°C until the OD600 reached at 0.6–0.8 ...
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bioRxiv - Systems Biology 2023Quote: ... Ligated plasmid products were transformed into stellar competent cells (E. coli HST08 strain, Takara Bio). See Table S1 for full descriptions of constructs.
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bioRxiv - Evolutionary Biology 2023Quote: ... recombinant protein (vector pColdTM TF-DNA, Takara Clontech) to use as the antigen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... recombinant protein (vector pColdTM TF-DNA, Takara Clontech) to use as the antigen ...
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bioRxiv - Microbiology 2024Quote: ... The recombinant construct was transformed into Escherichia coli StellarTM Competent Cells (dam–/dcm–) (Takara Bio) according to the supplier’s protocol (ClonTech ...
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bioRxiv - Systems Biology 2023Quote: ... was used as the wild-type (WT) strain for genetic manipulations. Chemically competent Escherichia coli (E. coli) DH5α and HST08 (Stellar Competent Cells, TaKaRa Bio Inc., Kusatsu, Shiga, Japan) were used as the host strains for molecular cloning.
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bioRxiv - Microbiology 2020Quote: ... Purification tags were removed by treating recombinant proteins with HRV3C protease (TaKaRa) and cOmplete™ His-tag Purification Resin (Roche) ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were purified with His60 Ni Gravity Column Purification Kit (Clontech) following a manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... coli HST08 (used to test coregulation of genes in newly-formed operons and for vector construction; E. coli HST08 Premium Competent Cells, Takara Bio, Japan, 9128).
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bioRxiv - Microbiology 2020Quote: ... coli (Clontech), extracted and retransformed into EAW19 ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant protein was purified using the His60 Ni gravity column purification kit (Takara Bio) according to the manual instruction ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins of interest were bound to TALON metal affinity beads (Clontech, Mountain View, CA) and eluted with imidazole (gradient 10–200 mM ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant Irg1-like protein was enriched using cobalt TALON Metal Affinity Resin (Takara Bio #635501) with gentle shaking for 1 hour at 4 ℃ and washed twice by centrifugation for 3 minutes at 3500 rpm at 4 ℃ and resuspension in the washing buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged recombinant proteins were isolated by 1 h incubation with Talon metal affinity resin (Clontech) at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... coli cells (Takara) and selected on LB-Ampicillin plates ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (TaKaRa) were transformed with these plasmids for amplification and DNA storage ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5α (Takara) or Stellar™ (Takara ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Clontech 636763) via heat shock ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coli (Takara,#9027) were transformed using electroporation and the subcloned library was obtained by combining 4 maxipreps ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5α (Takara) and Stellar™ (Takara) ...
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bioRxiv - Microbiology 2022Quote: ... coli Stellar (Takara) and E ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Takara Bio). Plasmids were purified by miniprep (Qiagen ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli Stellar (Takara) cells on LB- or M9 minimal-agar supplemented with 1 mM IPTG were inoculated into 250 μL of the same liquid medium and grown in a 1.5-mL 96-deep well plate at 240 rpm for ~10 generations ...
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bioRxiv - Genomics 2023Quote: ... coli (Takara Bioscience). 1% of the transformed cells were plated on ampicillin-agar plates to ensure adequate transformation efficiency (at least 10-fold library coverage) ...
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bioRxiv - Genomics 2023Quote: ... coli (Takara, 636763) and 10 colonies were picked at random to ensure that each colony was unique ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were expressed in Escherichia coli strain BL21(DE3) cells containing chaperone plasmid pG-KJE8 (TAKARA, 3340). When the optical density at 600 nm reached 0.6 ...
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bioRxiv - Biochemistry 2021Quote: ... After 12 h medium was collected and recombinant proteins were purified on TALON metal affinity resin (Takara). 200 μl of the resin was loaded onto 1 ml column (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... coli Stellar cells (Clontech/Takara Bio USA Inc. ...
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bioRxiv - Bioengineering 2019Quote: ... coli BL21 (DE3) (TaKaRa, hsdS ...
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bioRxiv - Biophysics 2020Quote: ... coli (TOYOBO or TaKaRa), calf intestine (Roche) ...
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bioRxiv - Bioengineering 2019Quote: ... coli cells (Stellar, TaKaRa) were used for all cloning steps and were grown in LB medium supplemented with antibiotics as required - ampicillin (100 μg/mL) ...
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bioRxiv - Microbiology 2021Quote: ... coli Stellar™ (Takara) following standard protocols [56] ...
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bioRxiv - Microbiology 2019Quote: ... coli strain StellarTM (ClonTech), prior to isolation and transformation into COH1 GBS ...
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bioRxiv - Microbiology 2019Quote: ... coli cells (Stellar, TaKaRa), grown in LB medium at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... coli cells (Stellar, TaKaRa). A synthetic version of the E ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (Takara Bio) and positive clones were identified via RFP selection before sequencing to confirm the cloning was successful.
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bioRxiv - Microbiology 2022Quote: ... coli Stellar cells (Takara) for non-R6K origin of replication-based vectors or PIR2 cells (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... coli HST08 (Takara Bio). The pLI50_pgl plasmid was then isolated and transformed by electroporation into the restriction-deficient strain RN4220 ...
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bioRxiv - Genomics 2022Quote: ... coli cells (Takara Bio) and plasmid was prepared following standard protocols ...
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bioRxiv - Microbiology 2022Quote: ... coli Stellar (Takara Bio) cells and plated on selective Luria-Bertani (LB ...
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bioRxiv - Developmental Biology 2023Quote: ... coli (Takara Bio, 636763) via ampicillin-resistant selection ...
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bioRxiv - Genomics 2023Quote: ... coli cells (Takara Bioscience). Sanger sequencing (Genewiz ...
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bioRxiv - Developmental Biology 2023Quote: ... coli (Takara Bio, 636763). DNA was extracted by miniprep (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... coli Stellar cells (Clontech) and plated onto Luria Bertani agar supplemented with 25 µg/ml chloramphenicol ...
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bioRxiv - Microbiology 2023Quote: ... coli StellarTM (Takara Biosciences) was used for cloning and plasmid storage ...