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Citations for Takara Bio :
1 - 50 of 461 citations for Protease Inhibitor Cocktail EDTA Free 100X in DMSO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and pH7.2) supplemented with the ProteoGuard EDTA-free protease inhibitor cocktail (Clontech, 635673). The cells in the suspended solution were sonicated using the Q125 sonicator (Qsonica ...
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bioRxiv - Microbiology 2022Quote: ... 2X ProteoGuard EDTA-free protease inhibitor cocktail (catalog no. 635673, Takara Bio USA, Inc., San Jose, CA, USA), and DNase and RNase-free RPI molecular grade water (catalog no ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell pellets were resuspended in the prepared cell lysis buffer (0.1% Triton-X, 20 mM Tris, 100 mM NaCl) and ProteoGuard EDTA-Free protease inhibitor cocktail (Takara) for 30 minutes followed by sonication in ice cold water for 40 minutes (75 amplitude ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X protease inhibitor cocktail (635673; Takara Clontech), 1 mM PMSF ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X protease inhibitor cocktail (635673; Takara Clontech), 1 mM PMSF ...
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bioRxiv - Plant Biology 2023Quote: ... 0.05% T-20 and protease inhibitor cocktail (Takara)] ...
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bioRxiv - Neuroscience 2023Quote: ... and 10% glycerol with complete protease inhibitor cocktail (Clontech Laboratories). Cells were incubated on ice for 20 minutes before centrifugation at 14,000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysozyme solution and Protease Inhibitor Cocktail (Takara Bio USA, Inc., CA). The suspension was sonicated 10 sec for 3 times with a 30 s pause ...
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bioRxiv - Cell Biology 2021Quote: ... protease inhibitor (Takara, Japan), primary antibody (Cell signaling technology ...
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bioRxiv - Cell Biology 2019Quote: ... 0.5% CHAPS supplemented with protease inhibitor cocktail) were first applied to a His60 Ni Superflow column (Clontech). Eluates from the His60 Ni column were pooled and dialyzed overnight against 50 mM sodium phosphate pH 7.4 and then loaded onto a DEAE column ...
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bioRxiv - Molecular Biology 2023Quote: ... Total protein was lysed by homogenization in radioimmunoprecipitation (RIPA) buffer (FUJIFILM) containing protease inhibitor cocktail (NACALAI TESQUE) and Cryonase Cold-active Nuclease (TAKARA), while total RNA was extracted using an RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... and protease inhibitors (Takara Bio, Kusatsu, Japan) mixes ...
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bioRxiv - Neuroscience 2020Quote: ... containing 1% protease inhibitor (Clontech, Mountain View, CA) and 1% phosphatase inhibitors (Millipore Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... containing 1% protease inhibitor (Clontech, Mountain View, CA) and 1% phosphatase inhibitors (Millipore Sigma) ...
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bioRxiv - Immunology 2023Quote: ... 0.1mM EDTA) supplemented with 0.2U/µl recombinant RNase Inhibitor (Takara, Catalog # 2313B). Nuclei were counted and kept on ice (or for longer storage at - 80°C) ...
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bioRxiv - Developmental Biology 2021Quote: ... Single embryos were collected in 2 µl RNase free water with Recombinant RNase inhibitor (TAKARA) and ruptured with an RNase free needle ...
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bioRxiv - Neuroscience 2022Quote: ... with RNAse-free water with 0.4 U/μL Recombinant RNase Inhibitor (Takara Clontech, Ref. 2313A) and a fresh dilution of 1 in 300,000 was prepared before the first strand synthesis.
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bioRxiv - Neuroscience 2022Quote: ... with RNAse-free water with 0.4 U/μL Recombinant RNase Inhibitor (Takara Clontech, Ref. 2313A) and a fresh dilution of 1 in 300,000 was prepared before the first strand synthesis.
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bioRxiv - Pharmacology and Toxicology 2023Quote: HepG2 cells were resuspended in ice-cold PBS supplemented with protease inhibitors (Clontech, Mountain View, CA, USA) and lysed in ice bath by sonication in cycles of 10 s on /5 s off for 1 min at 6–10 μm amplitude at 25 % intensity from an exponential ultrasonic horn of 3 mm in a Soniprep 150 MSE (MSE Ltd. ...
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bioRxiv - Immunology 2021Quote: ... 100,000 cells were sorted into 200 uL PBS with 1 uM DTT and 5 uL RNase Inhibitor Cocktail (Takara); for ex vivo culture experiments ...
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bioRxiv - Genomics 2022Quote: ... 16 µL 100x DAPI and 8 µL ICELL8 Second Diluent (Takara) were added and incubated 10 minutes at RT ...
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bioRxiv - Genetics 2022Quote: ... Produced lentiviruses were concentrated 100x with Lenti-X Concentrator (#631232, Takara) to increase virus concentration.
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bioRxiv - Molecular Biology 2022Quote: ... Produced lentiviruses were concentrated 100x with Lenti-X Concentrator (#631232, Takara) to increase virus concentration ...
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bioRxiv - Systems Biology 2020Quote: ... (2019) now recommend using Titanium Taq plus DMSO (Takara) and we have observed slightly better mapping rates for Titanium Taq amplicons ...
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bioRxiv - Immunology 2024Quote: ... Harvested supernatant was concentrated 100x using the Lenti-X Concentrator (Takara; 631231) and resuspended in complete DMEM and stored at -80°C ...
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bioRxiv - Microbiology 2023Quote: ... TwistAmp® Basic Kit (TwistDx, TABAS03KIT), Milenia HybriDtect1 (TwistDX, Milenia01), UltraPure DNase/RNase-Free Distilled Water (Thermo, 10977015) and RNase inhibitor (Takara, 2313A). Thermo Fisher Nanodrop 1000 Spectrophotom was used to quantify nucleic acid ...
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bioRxiv - Bioengineering 2023Quote: ... Lentivirus was then concentrated 100X using the Lenti-X concentrator (Takara Bio,631232). Biological titration of lentivirus by QPCR was carried out as previously described86 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.0 μl of 25mM B/B (in DMSO) (AP20187, Takara) was added to 50ul of PGCs (3,000 PGCs/μl ...
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bioRxiv - Genetics 2020Quote: ... 1.0 ul of 25mM B/B (in DMSO) (Takara Bio) was added to 50ul PGC suspension before injection and subsequently 50 μl 300ul P/S (containing 30ul of 0.5mM B/B drug ...
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bioRxiv - Developmental Biology 2020Quote: ... Lentivirus was first concentrated 100x using Lenti-X Concentrator kit from Takara (Cat # 631231). Concentrated lentivirus was aliquoted and stored in −80 before use ...
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bioRxiv - Neuroscience 2023Quote: ... HRV3C protease (TAKARA) was added overnight at 4°C and Ni-NTA was added to eliminate the cleaved portion overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Viral supernatant was collected at 48 hours and concentrated 100x using Lenti-X Concentrator (Clontech), resuspended in cell culture media ...
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bioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s instructions and concentrated ∼100x using the Lenti-X concentrator kit (Takara) and stored at -80°C until needed ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral media was then kept at 4°C before 100x concentration using LentiX concentrator (Takara #631232) and resuspension of virus in organoid seeding media ...
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bioRxiv - Bioengineering 2023Quote: ... The viral supernatant was concentrated to 100x in 1x DPBS using Lenti-X Concentrator (Clontech, 631232) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2019Quote: ... RNase inhibitor (Clontech), 2.5 mM dNTPs (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... RNAse Inhibitor (Takara), and ERCC RNA spike in controls (Ambion) ...
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bioRxiv - Immunology 2024Quote: ... RNAse Inhibitor (Takara)) in a 96-well V-bottom PCR plate (Thermo Fisher) ...
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bioRxiv - Systems Biology 2019Quote: ... and RNase-free water (TaKaRa, Japan)) in a 96-well PCR plate (BIOplastics).
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bioRxiv - Genomics 2019Quote: ... the beads were washed twice with 50 μl 80% freshly-prepared EtOH on the magnetic stand and the dried beads pellets were resuspended in 4 μL elution buffer (RNAse inhibitor 1/200 Takara, CDS primer 0.5 μL in RNAse free water) immediately followed by an incubation at 72°C for 3 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and the indicated type and amount of RNase Inhibitor (no RNAse inhibitor, 0.003 μl RNase Inhibitor (40 U/μl, Cat. 2313B, TaKaRa), SEQURNA Thermostable RNase inhibitor (Cat ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... We froze 5 μL of the supernatant in 45 μL of Tris-EDTA buffer (10 mM Tris, 1 mM EDTA, Takara Bio Inc.) before analysis by polymerase chain reaction (PCR) ...
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bioRxiv - Genomics 2024Quote: ... Supernatant containing viral particles was harvested after 2 and 3 days and concentrated 100x using Lenti-X concentrator (Takara) following manufacturers instructions.
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant RNase inhibitor (Takara) and protease inhibitor cocktail (Sigma-Aldrich)) ...
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bioRxiv - Developmental Biology 2020Quote: ... RNAse inhibitor (Clontech 2313A) at 1 units/μL and DTT (Promega P1171 ...
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bioRxiv - Developmental Biology 2020Quote: ... RNAse inhibitor (Clontech 2313A) at 1 units/μL and DTT (Promega P1171 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNase inhibitor (Takara Bio) and nuclease-free water ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the RNAse inhibitor (Clontech) was added during the each step of the nuclear preparation ...
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bioRxiv - Cell Biology 2021Quote: ... RNase inhibitor (Takara, Japan), protease inhibitor (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... cell-free protein expression system from Takara Bio Inc ...