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Citations for Takara Bio :
1 - 50 of 419 citations for Prestained Protein Peptide Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Plasmid pTAP-AFAP was constructed by cloning the gene fragment encoding AFAP-streptavidin-binding peptide-3xFLAG tag fusion protein (AFAP-SBP-3xFLAG, shorted as AFAP-SF) into mammalian expression vector pIRESpuro3 (Takara). SBP is a peptide with the length of 38 amino acids (Wilson et al. ...
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bioRxiv - Cell Biology 2019Quote: ... with bovine serum albumin (BSA) standard (Takara). The antibodies used in this research are described Table S2.
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bioRxiv - Immunology 2023Quote: ... Plates were coated with 5μg/mL of target peptide using coating reagent from the Takara Peptide Coating Kit (Takara cat. #MK100). Measles peptide was utilized as a negative control ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA ligation was done using standard ligation techniques (Takara DNA Ligation kit ver.2.1 ...
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bioRxiv - Molecular Biology 2020Quote: DNA ligation was performed using standard ligation techniques (Takara DNA Ligation kit ver ...
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bioRxiv - Cancer Biology 2020Quote: ... using standard In-Fusion cloning (Takara Bio, Cat # 638911) to create pLEGB ...
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bioRxiv - Immunology 2021Quote: ... Standards were prepared from a PCR PrimeStar MAX (Takara) reaction using naive C57BL/6OlaHsd mice genomic DNA for the internal control or donor AAV plasmid for the Donor sample and purified using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Plant Biology 2024Quote: ... following standard procedures (Yeast Protocols Handbook PT3024-1, Clontech). The proteins were extracted by post-alkaline methods59 and detected by western blot with antibody Myc (Abmart ...
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bioRxiv - Genomics 2021Quote: ... DNA standards for library quantification (Takara Bio Inc., Shiga, Japan) were used as the standard ...
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bioRxiv - Plant Biology 2023Quote: ... and standard PCR was performed using ExTaq HS kit (TaKaRa). Control reactions were run using wild-type DNA as a template.
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bioRxiv - Molecular Biology 2021Quote: ... and 1 µM of Shield-1 peptide (Clontech, Mountain View CA) for 4-6 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μM of Shield-1 peptide (Clontech, Mountain View CA) for 4 h ...
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bioRxiv - Genetics 2020Quote: ... RNA was extracted from the worms using the standard TRIzol (Takara) method ...
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bioRxiv - Bioengineering 2019Quote: ... MMEJ vector was modified so that 2A peptide and EGFP cDNA (Clontech) were inserted downstream of the SaCas9 driven by CMV promoter replaced for the GRK1-93 promotor ...
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bioRxiv - Microbiology 2020Quote: ... Transformation with plasmids and cultivation were performed using standard techniques (Clontech manual). In addition to negative and positive controls included in the MatchMaker III system ...
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bioRxiv - Cancer Biology 2022Quote: ... Bacterial transformation was performed following standard protocols using Stellar competent cells (Takara). Mini- and MidiPreps (Macherey-Nagel ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The standard curves were drawn using serially diluted pT7Blue (Takara Bio, Japan) plasmid carrying a kanamycin resistance gene fragment ...
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bioRxiv - Synthetic Biology 2023Quote: Standard PCR reactions were performed using the PrimeSTAR Max DNA Polymerase (TaKaRa) or KOD One PCR Master Mix -Blue-(Toyobo) ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed as standard procedures in 10 μl reactions by TaKaRa Ex Taq ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed as standard procedures in 50 μl reactions by TaKaRa LA Taq ...
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bioRxiv - Cell Biology 2021Quote: ... or cloned (all others) by standard mutagenesis techniques in the plasmid pQCXIP (Clontech). WT human PRRT2 (gene ID ...
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bioRxiv - Neuroscience 2023Quote: ... The standard curve was prepared using the positive control solution provided by Takara with serial dilution for absolute quantification ...
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bioRxiv - Genomics 2024Quote: ... Embryos were fixed and stained using standard Drosophila methods using anti-dsRed (Clontech) and visualized using the ABC-HRP kit (VectorLabs ...
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bioRxiv - Physiology 2019Quote: ... an exogenous RNA control (λ polyA External Standard Kit, Takara Bio Inc, Shiga, Japan) was added at a fixed amount (0.04 ng × ml−1 of Trizol reagent ...
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bioRxiv - Biochemistry 2020Quote: Standard recombinant DNA techniques and In-Fusion® HD Cloning (Takara Bio USA, Inc.) were used for plasmids construction in this study and were verified by DNA sequencing ...
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bioRxiv - Plant Biology 2023Quote: Cloning of RAP2.4pro:RAP2.4–venusYFP was done according to standard In–fusion cloning procedures (Takara In–Fusion® Snap Assembly Master Mix #638948) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were measured using BCA Protein Assay Kit (TaKaRa). The samples were subjected to SDS-PAGE ...
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bioRxiv - Plant Biology 2022Quote: ... benthamiana according to a standard AcLi-mediated transformation kit (Matchmaker; Clontech, Mountain View, CA, USA). To repeat the interaction ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Protein concentrations were assessed with the BCA protein assay kit (Takara BCA Protein Assay Kit, Takara, Shiga, Japan). SDS-PAGE and Western blotting were performed as previously described (Shintani et al. ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Protein concentrations were assessed with the BCA protein assay kit (Takara BCA Protein Assay Kit ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were estimated using a BCA Protein Assay Kit (Takara). For FRAP experiments ...
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bioRxiv - Cell Biology 2019Quote: ... 2016) using anti-GFP (Living Colors A.v. peptide antibody, rabbit polyclonal, 1 mg/ml; TakaraBio/Clontech) primary antibody and horseradish peroxidase linked anti-rabbit IgG (from donkey ...
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bioRxiv - Cell Biology 2023Quote: Procollagen Type 1 C-peptide (PIP) was measured according to the manufacturer’s instructions (Takara, Shiga, Japan). For evaluation of PIP ...
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bioRxiv - Molecular Biology 2019Quote: ... These overlapped fragments were then cloned into competent cells with standard In-Fusion HD Cloning (TaKaRa) techniques ...
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bioRxiv - Cell Biology 2019Quote: All plasmids were generated using standard restriction enzyme cloning and InFusion system (TaKaRa Bio, Kusatsu, Japan). The list of all plasmids used in the study is available in Table S1 ...
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bioRxiv - Developmental Biology 2023Quote: ... under standard conditions with oligo(dT) or random hexamer primers and Recombinant RNase Inhibitor (RRI, TAKARA). Then the cDNA was subjected to quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured using the BCA Protein Assay Kit (Takara Bio). The protein samples were subjected to SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration was determined by a BCA protein assay kit (TakaRa) using bovine serum albumin (BSA ...
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bioRxiv - Physiology 2022Quote: ... Protein concentration was determined using the BCA protein assay kit (Takara Bio).
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bioRxiv - Molecular Biology 2020Quote: ... and cloned downstream of a sequence encoding the CD8 signal peptide (MALPVTALLLPLALLLHAA) in the vector pIRESpuro3 (Clontech). BC-deltaSTP-GPR56 was from Lei Xu (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: ... An aliquot of the glycopeptide fraction was treated with peptide-N-glycanase F (PNGaseF, Takara, Shiga, Japan) in H218O to remove N-glycans and to label glycosylated Asn with 18O as Asp (18O) ...
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bioRxiv - Cell Biology 2019Quote: ... The expressed peptide was purified using hexa-histidine-tag and GST-tag by Talon-resin (Clontech/Takara) or Glutathione-sepharose (GE healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... The expressed peptide was purified using hexa-histidine-tag and GST-tag by Talon-resin (Clontech/Takara) or Glutathione-sepharose (GE healthcare ...
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bioRxiv - Synthetic Biology 2020Quote: ... Protein concentrations were measured with a BCA protein assay kit (Takara, Dalian, China).
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was quantified using the BCA protein assay kit (Takara Bio, T9300A). Cell lysates (about 200 µl ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or with standard (DreamTaq - Thermo Fisher Scientific, or EmeraldAmp Max HS PCR Master Mix - TaKaRa Bio Inc) DNA polymerases ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA ligation was done using standard ligation techniques (Takara DNA Ligation kit ver.2.1, Takara, Otsu, Japan). For the transformation ...
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bioRxiv - Biochemistry 2022Quote: ... aIF1A was purified as described (24) except that a standard affinity chromatography step on Talon resin (Clontech) was added to the original protocol ...
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bioRxiv - Microbiology 2020Quote: ... Western blotting was performed using standard procedures with the following primary antibodies: JL-8 monoclonal antibody (Takara) for GFP variants ...
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bioRxiv - Molecular Biology 2020Quote: DNA ligation was performed using standard ligation techniques (Takara DNA Ligation kit ver. 2.1; Takara, Otsu, Japan). For transformation ...