Labshake search
Citations for Takara Bio :
1 - 50 of 248 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: Tissue protein lysates were purchased from Takara. Details for each lysate are shown in Table S1 ...
-
bioRxiv - Genomics 2022Quote: Whole bladder protein lysate was obtained by homogenizing bladder tissue using Biomasher II (TaKaRa) in ice-cold RIPA buffer with the protease inhibitor (ThermoFisher Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 U/μL ribonuclease inhibitor (porcine liver) (TaKaRa Bio), 0.25 U/μL AMV reverse transcriptase XL (TaKaRa Bio) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.2 U/μL of ribonuclease inhibitor (porcine liver) (TaKaRa Bio) in 44.5 μL of the RNase-free Buffer RDD was added to each sample ...
-
bioRxiv - Cell Biology 2022Quote: ... Total RNA was extracted from crushed tissue or cell lysate using the TRIzol reagent protocol (TAKARA/Thermo). cDNA was synthesized using Superscript III (Thermo) ...
-
bioRxiv - Cell Biology 2021Quote: Isolating the porcine total RNA by Trizol reagent (TaKaRa, Dalian, China).
-
bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from brain tissues and cell lysates using an RNAiso Plus reagent (Takara, Shiga, Japan) and cDNA was synthesized using ReverTra Ace qPCR RT Master Mix (Toyobo ...
-
bioRxiv - Microbiology 2020Quote: ... The lysate was incubated with Talon Resin (CLONTECH) for 1 h at 20°C ...
-
bioRxiv - Microbiology 2020Quote: ... The lysate was incubated with Talon Resin (CLONTECH) for 1 h at 20°C ...
-
bioRxiv - Cell Biology 2019Quote: ... Lysates were then added to TALON ® (TaKaRa) resin and incubated for 1 hour at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Clarified lysates were incubated with HIS-60 (Takara) resin for 30-60 minutes ...
-
bioRxiv - Biophysics 2023Quote: ... Lysate was loaded on TALON resin (Takara Bio) gravity flow column ...
-
bioRxiv - Biochemistry 2019Quote: ... Cleared lysates were incubated with TALON resin (Clontech, 635504) which was preequilibrated with 50 mM Tris ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lysate was incubated with TALON metal affinity resin (Takara) (3ml slurry per L culture ...
-
bioRxiv - Microbiology 2021Quote: ... Clarified lysates were loaded onto a 1 mL TALON (Takara) column ...
-
bioRxiv - Biochemistry 2020Quote: ... The lysate was then incubated with 25μL NiNTA beads (Clontech) for 1 hour at 4°C for binding ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell lysate was mixed with SDS-PAGE loading buffer (Takara) containing 200 mM DTT ...
-
bioRxiv - Biochemistry 2020Quote: Clarified lysate was incubated with His60 Ni Superflow Resin (TaKaRa) for 1 hr at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Cleared lysate was subjected to purification using TALON resin (Clontech) which was equilibrated with 50 mM Tris ...
-
bioRxiv - Biochemistry 2020Quote: Clarified lysate was incubated with His60 Ni Superflow Resin (TaKaRa) for 1 hr at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: Clarified lysate was incubated with His60 Ni Superflow Resin (TaKaRa). Resin was washed with a buffer (300 mM NaCl ...
-
bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was added to TALON CellThru Resin (Takara) and incubated at 4°C for 1h ...
-
bioRxiv - Genomics 2024Quote: ... Lysates were incubated with His60 Ni Superflow resin (Takara Bio) at 4°C with rotation for 1 hour ...
-
bioRxiv - Biochemistry 2020Quote: ... CST was purified from the clarified lysate using Talon resin (Clontech) followed by protease cleavage of the GFP-His10 tag and finished with size exclusion chromatography on a Superdex 200 column equilibrated in Buffer A (25 mM HEPES pH 7.5 ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was applied to 2 ml Talon Superflow resin (Clontech) pre-equilibrated with buffer A (20 mM Tris/HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2021Quote: ... Clarified lysate was incubated with 5 mL TALON beads (Takara Bio), washed with 150 mL lysis buffer and eluted in 22 mL of elution buffer (25 mM Hepes pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... Cleared lysate was incubated with 400 μl of Talon Resin (TaKaRa) for 1 hour at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... and cell lysate passed over a TALON metal affinity column (Takara). After washing the column with Buffer I ...
-
bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was then bound to cobalt-charged resin (Takara) for 1hr and washed twice with lysis buffer ...
-
bioRxiv - Genomics 2022Quote: ... Tissues were homogenized using BioMasher II (TaKaRa) with RLT Plus buffer (1% β-mercaptoethanol).
-
bioRxiv - Molecular Biology 2023Quote: ... Cleared lysates were applied to 3 mL TALON Metal Affinity Resin (Takara) and incubated for 30 minutes at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... lysate clarified by centrifugation was passed over TALON Metal Affinity Resin (Takara), washed with 10 column volumes (CV ...
-
bioRxiv - Microbiology 2023Quote: ... Clarified lysates were incubated with TALON Metal Affinity Resin cobalt beads (Takara), and beads were then washed with Tris-HCL (pH 8.0 ...
-
bioRxiv - Microbiology 2023Quote: ... The lysate was added to the Ni-IDA resin (Takara, Cat # 635660) equilibrated with Binding Buffer (50 mM NaPi ...
-
bioRxiv - Genetics 2019Quote: ... liver and kidney), mouse tissues (brain, liver, heart, testis and kidney) and rat tissues (brain, liver and kidney) were purchased from Takara Bio Inc.
-
bioRxiv - Genetics 2020Quote: ... the pooled cDNA samples of human foetal tissues (Fetal MTC Panel, 636747, Lot#1308272A) and adult tissues (MTC panel I, 636742, Lot#1303120A) were sourced from Clontech. The cDNA samples from whole human embryonic and foetal hearts of different developing stages were obtained from the MRC-Wellcome Human Developmental Biology Resource (HDBR) ...
-
bioRxiv - Cell Biology 2020Quote: ... Clarified lysate was added to pre-equilibrated TALON® cobalt resin (Takara Bio) and incubated end-over-end for 1 h at 4 °C ...
-
bioRxiv - Synthetic Biology 2022Quote: ... the clarified lysate was bound to TALON® Metal Affinity Resin (Takara Bio) for 1 h at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... Diluted lysate was incubated with 1.25 ml His60 Ni Superflow affinity resin (Takara) per liter of expression culture ...
-
bioRxiv - Microbiology 2020Quote: ... and 2 µl of lysate was used for PCR using SapphireAMP (Takara, RR350) and gene-specific primers ...
-
bioRxiv - Biochemistry 2022Quote: ... Clarified lysates were incubated with TALON® metal affinity resin (Takara Bio USA) for 1 h with end-over-end rotation at 4 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Lysates were cleared by centrifugation and immobilized on TALON metal affinity resin (Takara) per the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... Clarified lysate was mixed with ∼0.5 ml/L of culture cobalt resin (TaKaRa) for one hour ...
-
bioRxiv - Systems Biology 2024Quote: ... We used 600 µl of cell lysate for total RNA extraction (Clontech 740955), followed by processing for microarray (for interactome assembly ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 2 µl of lysate was used for PCR using SapphireAMP (Takara, RR350) and primers specific for each gene ...
-
bioRxiv - Biochemistry 2023Quote: ... the clarified cell lysate was incubated with pre-equilibrated Ni- NTA resin (Takara) for 2 h at 4°C in slow shaking conditions ...
-
bioRxiv - Microbiology 2020Quote: ... and kidney using a NucleoSpin tissue kit (Clontech). Quantification of Leptospira 16s rRNA was done using TAMRA probe and primers from Eurofins (Huntsville ...
-
bioRxiv - Genetics 2021Quote: ... The clarified lysate was subjected to Talon Metal Affinity column (Takara, Mountain View, CA) as per the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... target proteins were purified from cell lysate by Ni-iminodiacetic acid affinity chromatography (Clontech). However ...
-
bioRxiv - Biophysics 2019Quote: ... and proteins were purified from the lysate with the His60 Ni Superflow Resin (Clontech). An additional purification step was done through size exclusion chromatography in a Superdex 200 FPLC column (GE Healthcare).