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Citations for Takara Bio :
1 - 48 of 48 citations for PDGF Receptor Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: PDGF-IRES-Cre was generated by cloning human PDGF-B and Cre into pQXIX vector (Clontech) as previously described5 ...
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bioRxiv - Bioengineering 2022Quote: ... PDGF-aa (20 ng/ml; R&D) is also supplemented to RHB-A (Takara). This medium composition will be referred to as RHB-A complete ...
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bioRxiv - Immunology 2021Quote: ... with the modification that Ecotropic Receptor Booster (Takara, #631471) was added to the cells as suggested by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... ECL substrate (TakaRa) was added and developed onto X-ray film.
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bioRxiv - Plant Biology 2023Quote: ... HRP substrates (Takara) and an iBrightTM CL750 Imaging System were used for immunodetection.
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bioRxiv - Neuroscience 2023Quote: ... was similarly constructed with the leptin receptor promoter controlling expression of mCherry (Clontech) without ChR2 ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoreactive bands were luminescent in a chemiluminescent substrate (Western BLoT Quant HRP substrate, cat. # T7102A, TaKaRa) and imaged using an image analyzer (LAS4000 ...
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bioRxiv - Plant Biology 2020Quote: ... as substrate (Yeast Protocols Handbook, Clontech). For UV-B treatments ...
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bioRxiv - Immunology 2022Quote: ... 3,3’-diaminobenzidine (DAB) substrate (Takara, Japan) was added ...
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bioRxiv - Bioengineering 2024Quote: ... and the receptor- or payload-encoding viral expression plasmids (2.0 μg) into Lx293T cells (Clontech) in 6-well plates with Lipofectamine 3000 (Thermo Fisher L3000001) ...
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bioRxiv - Plant Biology 2021Quote: ... as substrate as described (Yeast Protocols Handbook, Clontech).
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bioRxiv - Plant Biology 2023Quote: ... Probes were detected with sensitive HRP substrates (Takara), and an iBrightTM CL750 Imaging System.
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bioRxiv - Immunology 2021Quote: ... or Western BLoT Ultra Sensitive HRP Substrate (Takara Bio).
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bioRxiv - Plant Biology 2022Quote: ... Bound proteins were detected using Hyper HRP Substrate (Takara). PtIns(3,5)P2 Grip protein (P-3516-3-EC ...
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bioRxiv - Cancer Biology 2023Quote: ... and developed using Western BLoT Chemiluminescent HRP Substrate (Takara). The chemiluminescent images were observed using FUSION FX (Vilber ...
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bioRxiv - Molecular Biology 2024Quote: ... Signals were detected with ECL western blotting substrate (TaKaRa) and image was acquired using Azure Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... Insoluble material was removed by centrifugation and the supernatant containing the receptor was rotated with Talon resin (Takara) overnight ...
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bioRxiv - Microbiology 2021Quote: ... or Western BLoT Ultra Sensitive HRP Substrate (Takara, Cat# T7104A) according to the manufacturers’ instruction ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were transiently transfected either with GluK3EM or co-tansfected with Wild type/mutant receptors along with GFP expressing plasmid (2 µg/dish) using Xfect reagent (Clontech). Currents were recorded from medium sized cells expressing a moderate level of fluorescence from either the fused EGFP in case of GluK3EM or co-expressed EGFP and having a capacitance of ∼5-6 pF at 48-60 hours post transfection ...
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bioRxiv - Immunology 2020Quote: ... The entirety of each of the annotated intracellular domains was ordered as a separate gene fragment (Integrated DNA Technologies) and each complete receptor was cloned into pHR lentiviral expression vector (Clontech). Each region of the protein was amplified using PCR and fragments were combined using Gibson assembly.
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bioRxiv - Immunology 2023Quote: T cell receptors were generated by custom synthesis (Twist Bioscience) in a 3rd generation pLVX-EF1α-IRES-Puro lentiviral vector (Takara/Clontech). Following the TransIT-Virusgen Transfection (Mirus ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells and hippocampal neurons were transfected with plasmids encoding FM4 recombinant receptors for 15 h and then treated with 2 μM DD-Solubilizer (TakaraBio/Clontech) to induce the release of the receptors from the ER into the secretory trafficking ...
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bioRxiv - Neuroscience 2023Quote: ... Enhanced chemiluminescence (ECL) substrate and stripping buffer were purchased from Takara Bio (Kusatsu ...
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bioRxiv - Immunology 2023Quote: T cell receptors were generated by custom synthesis (Twist Bioscience) in a 3rd generation pLVX-EF1α-IRES-Puro lentiviral vector (Takara/Clontech). Following the TransIT-Virusgen Transfection (Mirus ...
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bioRxiv - Immunology 2021Quote: ... immunoreactive bands were visualized using Western BLoT Quant HRP Substrate (Takara Bio) or Western BLoT Ultra Sensitive HRP Substrate (Takara Bio).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane was incubated with Western BLoT Chemiluminescence HRP Substrate (TaKaRa Bio), and membrane image was captured using a chemiluminescent imaging system LuminoGraph I (ATTO ...
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bioRxiv - Microbiology 2023Quote: ... Chemiluminescence was detected using Western BLoT Ultra Sensitive HRP Substrate (T7104A, Takara) according to the manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Site-directed mutagenesis in GC-A and GC-B and construction of chimeric receptors were performed through use of the In-Fusion HD plus cloning kit (Takara Bio Inc.). The primers used are listed in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified fragments required to generate the chimeric receptor were combined with a linearized pCS2+ backbone and annealed using an In-Fusion kit (Takara Bio, 638947). The resulting plasmids were transformed into Top10 chemically competent cells ...
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bioRxiv - Bioengineering 2019Quote: ... The ultrasensitive HRP substrate used for Western blotting was from TaKaRa (Shiga, Japan). The CM5 Sensor Chip ...
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bioRxiv - Microbiology 2021Quote: ... The protein bands were visualized using the Western BLoT Hyper HRP Substrate (TAKARA) and exposed using a Chemiluminescence Imaging System (Fusion Solo S ...
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bioRxiv - Cell Biology 2021Quote: The membrane was then incubated with Western BLoT Hyper HRP Substrate (Takara: T7103B) for chemiluminescence detection ...
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bioRxiv - Immunology 2022Quote: ... The signal was detected using the Western Blot Ultra-Sensitive HRP Substrate (Takara) and imaged using the Fusion FX Imaging system (PeqLab Biotechnologie).
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bioRxiv - Biochemistry 2023Quote: ... The annealed substrate was purified using CHROMA SPIN™+TE-200 Columns (TaKaRa) to remove unannealed oligonucleotides.
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with Takara POD conjugate solution and DAB substrate (Takara, Japan).
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bioRxiv - Immunology 2021Quote: ... T cell receptor sequencing libraries were prepared with the SMARTer Human TCR α/β Profiling Kit (catalog number 635015, Takara Bio USA, Inc.) according to manufacturer’s instructions with the exception of excluding the third and fourth bead size selection steps listed in Table 3 of the kit manual ...
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bioRxiv - Molecular Biology 2020Quote: The cleavage substrate was amplified using overlap-PCR (PrimeSTAR GXL DNA Polymerase from Takara) from the random PAM plasmid library (see Supplementary Materials and Methods) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the blot was developed with Western BLoT Chemiluminescence HRP Substrate (Takara, Kusatsu, Shiga, Japan), and the signals were detected using the ChemiDoc XRS+ Imager (Bio-Rad).
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bioRxiv - Plant Biology 2020Quote: ... Immunodetection was performed by incubating the membranes in the Western BLoT Quant HRP Substrate (Takara) and recording the chemiluminescence by LuminoGraphI(ATTO).
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bioRxiv - Biophysics 2020Quote: ... 50 µl of cell lysate was added to equal volume of LDH substrate (Takara, France) in a 96-well microplate and incubated at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed and quantitative β-galactosidase assay was performed using ONPG substrate following standard protocols (Clontech Laboratories). For two-hybrid experiments in meiotic conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed and quantitative β-galactosidase assay was performed using ONPG substrate following standard protocols (Clontech Laboratories).
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bioRxiv - Genomics 2022Quote: ... in which 5 pmol of substrate primer and 12,5 µl of TB Green Premix Ex Taq II (Takara) were added ...
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bioRxiv - Genetics 2023Quote: ... The phosphorylated reverse strand from each substrate was digested by sequential strandase-treatment to yield ssDNA (Takara 632666) which was column-purified and resuspended in nucleases-free water (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Supernatants (50 μL) were then combined with an equivalent volume of LDH reaction buffer/substrate mix (Takara Bio Inc.) in a clear-bottom 96-well plate ...
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bioRxiv - Plant Biology 2022Quote: ... β-galactosidase activity in liquid cultures was assayed using CPRG as substrate as described in the Yeast Protocols Handbook (Takara).
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bioRxiv - Biochemistry 2022Quote: ... Normalization was made by measuring the β-Gal activity after 1-hour incubation of 7.5 µl of cellular extract with 50 µl of β-Gal substrate (β-Gal detection kit II, Clontech).
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Quantification of transcript was done by qRT-PCR with fluorescent-labelled nucleotide substrate (TB Green™ Premix Ex Taq™ II, Takara or PowerUp SYBR Green Master Mix ...