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Citations for Takara Bio :
1 - 50 of 2681 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1× ExTaq PCR buffer (Takara, Clontech Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... 1× ExTaq PCR buffer (Takara, Clontech Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... 1x of PCR buffer (10x, Takara, CA, USA), 10 μL of barcoded primer set forward/ reverse from ONT ...
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bioRxiv - Cancer Biology 2023Quote: For indexing PCR, 78μL PCR mix (24μL H20, 50μL 2X SeqAmp CB PCR buffer (Takara Cat. #638526), 2μL SeqAmp DNA polymerase ((Takara Cat ...
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bioRxiv - Microbiology 2020Quote: ... Each PCR reaction contained 2.5 µl of PCR buffer (TaKaRa, 10x concentration, 1x final), 1 µl of the Golay barcode tagged forward primer (10 µM concentration ...
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bioRxiv - Neuroscience 2023Quote: ... Clontech Advantage PCR buffer (1x) and mix (1x) (TaKaRa), linker-ligated DNA (0.6 v/v ratio) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR fragments were annealing in 1X prime star buffer (Takara) and Surveyor enzyme was added to digest the annealed DNA fragments at 42 °C for 1h ...
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bioRxiv - Microbiology 2021Quote: ... 10 μL of 2× One Step RT-PCR buffer (TaKaRa), and 5 μL ddH2O ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.6 μl PCR mix was dispensed to each well containing the following: 1× SeqAmp PCR buffer (Takara Bio), 0.025 U μl−1 of SeqAmp polymerase (Takara Bio ...
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bioRxiv - Molecular Biology 2020Quote: ... Reaction conditions were as follows: 5 μL 10× PCR buffer (Takara Bio), 5 μL dNTPs (25 mM ...
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bioRxiv - Plant Biology 2021Quote: ... PCR was conducted with TaKaRa LA Taq® with GC buffer (TaKaRa) as follows ...
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bioRxiv - Genomics 2019Quote: The 90-μL preparation volume for PCR contains 1X Advantage 2 PCR Buffer [not short amplicon (SA)](10X, Clontech, 639206, Advantage® 2 PCR Kit), 0.4-mM dNTP Mix (50X/10 mM ...
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bioRxiv - Neuroscience 2022Quote: ... washed with 200 µL water, and resuspended in PCR mix (22 µL water, 25 µL Terra PCR direct buffer, 1 µL Terra Polymerase (Takara), 1 µL 100 M Truseq PCR handle primer ...
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bioRxiv - Plant Biology 2021Quote: ... Each PCR was conducted using TaKaRa LA Taq® with GC buffer (TaKaRa), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... PCR amplification was conducted using TaKaRa LA Taq® with GC buffer (TaKaRa), gene specific primers (300 nM of each) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR reaction included 2.5 µL of 10X buffer (Mg2+ plus) (TaKaRa, Japan), 1.5 µL of 25 mM MgCl2 ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with 2x SeqAMP buffer and SeqAmp DNA polymerase (Takara USA) and for the final amplification ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with 2x SeqAMP buffer and SeqAmp DNA polymerase (Takara USA) and for the final amplification ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR reaction mixture for all reactions consisted of 5x PrimeSTAR Buffer (Takara), 200uM dNTPs (Takara) ...
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bioRxiv - Biochemistry 2021Quote: ... total DNA was extracted from muscle tissue using Lysis Buffer for PCR (Takara, Shiga, Japan) and Proteinase K (Takara) ...
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bioRxiv - Neuroscience 2023Quote: ... and its contents were expelled into a PCR tube containing the lysis buffer (Takara, 634894). Metadata for all Patch-seq neurons including in this study are located in Supplementary Data Table 3.
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bioRxiv - Neuroscience 2020Quote: ... The contents of the pipette were expelled into a PCR tube containing lysis buffer (Takara, 634894). cDNA libraries were produced using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Semi-quantitative PCR was conducted using LA taq with GC buffer I (Takara medical co. ltd) using the methods in manual.
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bioRxiv - Cell Biology 2020Quote: ... gDNA of CFU colonies was extracted by suspending the cell in 1×PCR buffer (Takara, R050A) containing 0.2mg/ml proteinase K (Tiangen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and deposited into a PCR tube with 5 μL lysis buffer (Takara Bio, San Francisco, CA). cDNAs were then prepared by reverse transcriptase (Takara Bio ...
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bioRxiv - Microbiology 2019Quote: ... the cDNA was purified and eluted in 20ul of elution buffer (NucleoSpin PCR Clean-up Kit, Clontech). The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... cells were single-cell sorted into 96-well low-bind PCR-plates [Eppendorf] containing 3 μl of lysis buffer (0.5 units/μl RNase inhibitor [Takara] ...
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bioRxiv - Microbiology 2020Quote: ... 4 μL of 10 × PCR Buffer (Mg2+ plus) provided with the TAKARA Taq™ Hot Start Version (TAKARA, Japan), 0.08 μL of TransScript® Reverse Transcriptase [M-MLV ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The 16S rRNA V4 region was PCR-amplified with buffer and polymerase PrimeSTAR GXL DNA Polymerase (Takara, Cat: R050B). The cycling conditions for the PCR are as follows:_98 °C for 3 min ...
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bioRxiv - Neuroscience 2021Quote: ... cytosol and/or nucleus content in each pipette were expelled into individual PCR tubes containing 11.5 µl of lysis buffer (Takara, 634984) and stored in −80 °C.
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bioRxiv - Genetics 2023Quote: ... The whole fkbA locus from the FK506 resistant isolates was amplified using primers JOHE52223 and JOHE52224 and LA Taq DNA polymerase for long-range PCR with GC Buffer I (Takara). PCR conditions were optimized for long fragments as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA amplification was performed by adding 15 μL of Seq amp PCR mixture (12.5 μL of 2x SeqAmp buffer (Takara Bio), 0.05 μL of 100 μM N-IS PCR primer ...
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bioRxiv - Neuroscience 2023Quote: ... we harvested 10-12 GFP+ cells per fly into 0.5ul nuclease free water in the pipette tip and then the tip was broken into a 96 well PCR tube containing RNAse inhibitors and buffer as described by Clontech’s ultra low HV SMARTer Ultra Low RNAseq kit (Catalog #634823) ...
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bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...
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bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...
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bioRxiv - Neuroscience 2020Quote: ... cytosol and nucleus was removed from pipette holder and contents were expelled into a PCR tube containing the lysis buffer (Takara, 634894).
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bioRxiv - Neuroscience 2020Quote: ... and nucleus was removed from the pipette holder and contents were expelled into a PCR tube containing lysis buffer (Takara, 634894).
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bioRxiv - Neuroscience 2020Quote: ... The pipette was removed from the recording chamber and the contents of the pipette were expelled into a PCR tube containing lysis buffer (Takara, 634894). Patch-seq sample tubes were held on dry ice in a benchtop plexiglass enclosure throughout the recording session to ensure collected samples remained free of RNAse and DNAse contamination ...
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bioRxiv - Neuroscience 2020Quote: ... cytosol and nucleus was removed from pipette holder and contents were expelled into a PCR tube containing the lysis buffer (Takara, 634894).
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bioRxiv - Microbiology 2020Quote: ... 1 μl sense and 1 μl anti-sense primers of 100 μM each were mixed with 2 μl 10X Taq polymerase PCR buffer (Takara, Japan) and 16 μl ultra-pure water to a final volume of 20 μl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR reactions were performed in a 25μl-reaction mixture containing 12.5μl of 2 × Gflex PCR Buffer (Mg2+, dNTP plus) (TaKaRa Bio Inc., Shiga, Japan), 0.5μl of Tks
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bioRxiv - Neuroscience 2022Quote: ... and nucleus was removed from the pipette holder and contents were expelled into a PCR tube containing lysis buffer (Takara, 634894).
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bioRxiv - Cancer Biology 2023Quote: ... A bead ratio of 1x was used (50 µL of AMPure XP beads to 50 µL cDNA PCR product with 1 µL of 10x lysis buffer added, as per Clontech instructions), and purified cDNA was eluted in 17 µL elution buffer provided by Clontech ...
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bioRxiv - Microbiology 2021Quote: ... recombined by PCR (TaKaRa PCR kit (Clontech Laboratories)) using the primers and cycle conditions in Table S1C ...
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bioRxiv - Microbiology 2021Quote: ... recombined by PCR (TaKaRa PCR kit (Clontech Laboratories)) using the primers and cycle conditions in Table S1C ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR (CloneAmp HiFi PCR Premix, Takara-Bio Europe) genotyping was done with primers designed for each knock-out line that allowed for confirming either the presence of the wild-type (F-gt5/R-gt5WT and F-gt3WT/R-gt3 ...
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bioRxiv - Plant Biology 2023Quote: ... we PCR amplified (CloneAMP HIFI PCR premix, TAKARA) the coding sequences of ATG8a ...
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bioRxiv - Microbiology 2020Quote: ... The PCR reaction (20 µl) contained 1.25 U of TaKaTa ExTaq Polymerase and 1 x ExTaq Buffer (Clontech Laboratories, Palo Alto, CA, USA), 312.5 µM of each dNTP ...
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bioRxiv - Systems Biology 2022Quote: Following the transfer of samples into a 384-well plate containing RT-PCR buffer with 3’ SMART-Seq CDS Primer IIA (SMART-Seq® v4 PLUS Kit, TaKaRa, cat# R400753); the samples were immediately denatured at 72ºC for 3 min and chilled on ice for at least 2 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Klotho genotypes were determined by semi-quantitative PCR using LA-Tag DNA polymerase and TaKaRa buffer with Mg+2 (TaKaRa Shuzo, Tokyo, Japan) as follows ...