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Citations for Takara Bio :
1 - 29 of 29 citations for Nuclease, Micrococcal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Micrococcal nuclease (TaKaRa Bio Inc., Shiga, JP) was added to chromatin and digested at 25 °C for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... and 5U of Micrococcal Nuclease (Takara, 2910A) and incubated for 10 min at 37 °C ...
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bioRxiv - Genomics 2021Quote: ... and chromatin was then digested using Micrococcal nuclease (Takara) (1U per 10 μg chromatin ...
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bioRxiv - Immunology 2022Quote: ... NETs were treated with 2 U micrococcal nuclease (Takara Bio) for 10 min at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... The bead suspension was treated with 0.25 unit/µl Micrococcal Nuclease (Takara Bio) at 25°C for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... Co-purified cellular RNAs were degraded by incubating with 100U micrococcal nuclease (Clontech, 2910A) on-resin in 25 mL of Nickel wash buffer supplemented with 5 mM CaCl2 at room temperature for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Co-purified cellular RNAs were degraded by incubating with 100U micrococcal nuclease (Clontech, 2910A) on-resin in 25 mL of Nickel wash buffer supplemented with 5 mM CaCl2 at room temperature for 1 h ...
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bioRxiv - Biochemistry 2021Quote: Nucleosomes with the 145 base-pair DNA (200 ng each) were mixed with 0.01 unit/μL micrococcal nuclease (MNase; Takara) and incubated at 37°C for 0 ...
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bioRxiv - Biophysics 2020Quote: ... containing either tetra-acetylated or unmodified histone H4 was digested for 5 min at 22 °C with micrococcal nuclease (MNase) (0.125 to 2.0 units; Takara, cat. #2910A) in 5.5 mM Tris-HCl buffer (pH 7.6 ...
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bioRxiv - Plant Biology 2024Quote: ... were enriched from the lysate by immunoprecipitation using GFP-Trap (Lablead, GNM-25-1000) and were partially digested by micrococcal nuclease (2 × 10−5 U/μL, Takara, 2910A). The digested RNA was ligated to the 3′-RNA adaptor labeled by biotin ...
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bioRxiv - Bioengineering 2023Quote: ... RNA was resuspended in nuclease-free water (TAKARA) and then treated with DNAse using the TURBO DNA-free Kit (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... 19.5 µl of nuclease-free water (Takara Bio), and 5 µl of the DNA template ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA was digested with S1 nuclease (TaKaRa, Kusatsu, Japan) at 37°C for 20 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the amplified DNA samples were processed with S1 nuclease (TaKaRa) to reduce branching junctions.
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bioRxiv - Molecular Biology 2023Quote: ... RNA was incubated with 1 μL of S1 nuclease (Takara, Okasa, Japan) for 4 h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... DNA samples were digested with SacI and S1 nuclease (Takara Bio, #2410) and analyzed by agarose gel electrophoresis followed by staining with SYBR Gold nucleic acid gel stain (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... nuclease-free water and Titanium Taq DNA Polymerase (Clontech Laboratories Inc.; Takara Bio Group). Telomerase activity standard curves were generated using four 10-fold serial dilutions (0.02 ...
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bioRxiv - Cell Biology 2020Quote: ... nuclease-free water and Titanium Taq DNA Polymerase (Clontech Laboratories Inc.; Takara Bio Group). Telomerase activity standard curves were generated using four 10-fold serial dilutions (0.02 ...
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bioRxiv - Molecular Biology 2020Quote: ... 3.5 μL nuclease-free water was added to 4 μL of 5x PrimeScript buffer (Takara, USA), 1 μL RNAse OUT (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... DNA samples that were shortened to the appropriate size were blunted with S1 nuclease (Cat# 2140, Takara Bio) and T4 DNA polymerase (Cat# 2040 ...
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bioRxiv - Cell Biology 2019Quote: RNA from stress granules or cytosolic mRNAs from heated HeLa cells were resuspended in 10 μl of MS water and digested with 0.05 units of nuclease P1 (Wako) and 0.05 units of bacterial alkaline phosphatase (BAP C75, Takara) in P1 buffer (20 mM ammonium acetate pH 5.3 ...
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bioRxiv - Cell Biology 2020Quote: ... the sgRNA plasmid and a plasmid expressing the Cas9 nuclease were transfected using the Xfect Transfection Reagent (631318, Takara). A Cas9 expression plasmid without a puromycin resistance was generated by removing puromycin from pAc-sgRNA-Cas9 (addgene# 49330) ...
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bioRxiv - Genomics 2020Quote: ... The resulting plasmids were digested with Kpn1 and Xho1 at the multiple cloning site of the vector and serially deleted from the XhoI cutting site (5’-protruding end) by treating the plasmids with exonuclease III and mung bean nuclease (Takara) for fixed times ...
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Dissemination of linezolid-resistance through sex pheromone plasmid transfer in Enterococcs faecalisbioRxiv - Microbiology 2019Quote: ... bacterial cells harvested from fresh culture were embedded in agarose gels and then digested by S1 nuclease (TaKaRa, Dalian, China) at 37 °C for 20 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... The cDNA was diluted 1:1 with nuclease free water and subjected to qRT-PCR using SyBr green mix (Takara) in real-time PCR machine (BioRad CFX96) ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA from each candidate cell line was digested to single nucleoside using nuclease P1 (Fujifilm Wako Pure Chemical Corporation) and bacterial alkaline phosphatase (TaKaRa), followed by mass spectrometry analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... Total protein was lysed by homogenization in radioimmunoprecipitation (RIPA) buffer (FUJIFILM) containing protease inhibitor cocktail (NACALAI TESQUE) and Cryonase Cold-active Nuclease (TAKARA), while total RNA was extracted using an RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: ... Each cell was then aspirated a final time and deposited into an individual 0.5mL tube containing 9.5ul of nuclease-free water and 1ul of 10x Reaction Buffer (Smart-Seq V4 Ultra Low Input RNA kit, Takara Bio) and allowed to incubate at room temperature for 5 minutes before being stored at −80deg until cDNA synthesis ...
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bioRxiv - Developmental Biology 2021Quote: ... 350 ng RNA samples in 6 µL nuclease-free water) were generated using the Clontech SMARTer smRNA-Seq kit using 8 PCR cycles (Takara Bio). 30 µL of the PCR reaction was purified with AMPure XP beads (Beckman Coulter A63880 ...