Labshake search
Citations for Takara Bio :
1 - 50 of 505 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2020Quote: Human mitochondrial to nuclear DNA ratio kit (Takara) was used to assess mitochondrial DNA content ...
-
The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Yeast transformation as well as yeast protein extraction were performed following the recommendations presented in the Yeast Protocol Handbook PT3024-1 (Clontech).
-
bioRxiv - Cancer Biology 2022Quote: ... Prior to RNA extraction (Takara Nucleospin), tumors were crushed using ice-cold mortar and pestle under sterile conditions ...
-
bioRxiv - Genomics 2020Quote: ... MiniBEST Plant RNA Extraction Kit (TaKaRa) was used to isolate total RNA ...
-
bioRxiv - Immunology 2020Quote: ... and purified by gel extraction (Clontech Libraries).
-
bioRxiv - Plant Biology 2022Quote: Nuclear two-hybrid interaction assays were performed using the MATCHMAKER GAL4 two-hybrid system (Clontech). The CWLP coding region without signal peptide (SP ...
-
bioRxiv - Plant Biology 2021Quote: ... after-ripened Col and ref6c seeds collected at different imbibition time points were subjected for total RNA extraction using Minibest plant RNA extraction kit (Takara). Three independent biological replicates were performed ...
-
bioRxiv - Genetics 2022Quote: ... total RNA extraction and separation of residual DNA contamination were excuted using Plant RNA Extraction Kit (TaKaRa MiniBEST, kyoto, Japan), and RNase-free DNase I (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: Ribozol RNA extraction reagent was purchased from Takara Life Sciences and the Verso cDNA Synthesis Kit (Thermo Scientific) ...
-
bioRxiv - Immunology 2021Quote: ... Purification was performed with AAVpro Extraction Kit (Takara) according to the manufacturer protocol ...
-
bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells by TRIzol and an RNA extraction kit (MiniBEST Universal RNA Extraction Kit, RR820A, Takara, China). Total RNA (1 μg ...
-
bioRxiv - Genomics 2023Quote: ... purified via gel extraction from a 1% agarose gel followed by cleanup using the NucleoSpin PCR clean up and gel extraction kit (Takara Bio). Linearized CROP-seq-optiI and amplified oligonucleotides were assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB ...
-
bioRxiv - Zoology 2020Quote: ... MiniBEST Universal RNA Extraction Kit was purchased from TaKaRA, iScript™ gDNA Clear Synthesis Kit was purchased from Bio-Rad.
-
bioRxiv - Cell Biology 2022Quote: ... AAV particles were isolated using AAVPro extraction solution (Clontech). After transduction with both CRISPR/Cas9 gesicles and AAV homology donor ...
-
bioRxiv - Immunology 2020Quote: ... Purification was performed with the AAVpro Extraction Kit (Takara) according to manufacturer protocol and titer quantification by qPCR with SYBRGreen (ThermoFisher).
-
bioRxiv - Microbiology 2023Quote: ... followed by RNA extraction using NucleoSpin RNA Plus (TaKaRa).
-
bioRxiv - Molecular Biology 2023Quote: ... The TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan) was used to extract the total RNA from the total RNA of larval gut samples in the AcCK1 ...
-
bioRxiv - Immunology 2021Quote: ... 100 μL cell culture supernatant was harvested for viral RNA extraction using the MINIBEST Viral RNA/DNA Extraction Kit (Takara, Cat no. 9766). RNA was eluted in 30 μL RNase-free water ...
-
bioRxiv - Microbiology 2020Quote: ... One hundred microliter cell culture supernatant was harvested for viral RNA extraction using the MiniBEST Viral RNA/DNA Extraction Kit (Takara, Cat no. 9766) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... RNA extractions were performed using NucleoSpin® RNA XS (Takara) and resuspended in 20ul of RNase free water ...
-
bioRxiv - Microbiology 2021Quote: ... PSCs by Mini BEST Universal RNA Extraction Kit (Takara, Japan). Me Script II 1st Strand cDNA Synthesis Kit (Takara ...
-
bioRxiv - Developmental Biology 2023Quote: ... RNA extraction was performed with RNAiso Plus reagent (TaKaRa, AKF0727A). Transcriptome library construction and subsequent sequencing analysis were completed by Novogene Technology Co. ...
-
bioRxiv - Biochemistry 2024Quote: ... and RNA extraction kit were bought from Takara (Dalian, China). Ultrapure water was employed throughout the work ...
-
bioRxiv - Physiology 2019Quote: ... After total RNA extraction using Isogen (Takara Bio Inc., Shiga, Japan), the RNA quality was monitored using an Agilent 2100 Bioanalyzer system (Agilent Technology ...
-
bioRxiv - Plant Biology 2021Quote: ... following by phenol/chloroform extraction and precipitation with DNA mate (TaKaRa). The primers used for PCR were listed in Supplemental Table S1.
-
bioRxiv - Molecular Biology 2022Quote: ... Following purification using the MiniBEST agarose gel DNA extraction kit (TaKaRa), the digested DNA fragment and pET28a were ligated using the quick ligation kit (NEB) ...
-
bioRxiv - Microbiology 2022Quote: ... and plasmid isolation and gel extraction kits were obtained from Takara Biotech (USA) ...
-
bioRxiv - Microbiology 2021Quote: ... total cellular RNA was extracted using Universal RNA Extraction Kits (Takara). Then the Reverse Transcription Kit (Takara ...
-
bioRxiv - Microbiology 2019Quote: ... RNA extraction was performed using the RNAiso Plus (Takara, Dalian, China), and the first strand of cDNA synthesis was performed using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara ...
-
bioRxiv - Cell Biology 2023Quote: ... total RNA was isolated with MiniBEST Universal RNA extraction Kit (TaKaRa). 500 ng total RNA was saved as input sample ...
-
bioRxiv - Biochemistry 2023Quote: ... We purified PCR products by agarose gel extraction (Takara Bio, 740609) and used BP clonase (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: RNA extraction was carried out using RNAiso Plus (TaKaRa, Dalian, China) following the manufacturer’s instructions with some modifications ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... mtDNA copy number was determined by quantitative PCR with Human mitochondrial to nuclear DNA ratio kit (Takara Bio USA, 7246).
-
bioRxiv - Biochemistry 2020Quote: ... Full-length EGFP fused N-terminally to a nuclear localization signal (NLS) and C-terminally to a Myc-tag was expressed from pCMV (Clontech).
-
bioRxiv - Plant Biology 2023Quote: ... were PCR-amplified and fused to a nuclear localization signal (NLS) in pENTR vectors using In-Fusion HD Cloning Plus (Clontech). See Table S5 for primer details ...
-
bioRxiv - Molecular Biology 2023Quote: ... and those targeting the Alu repeat sequence in the nuclear genome (5′-CTTGCAGTGAGCCGAGATT-3′ and 5′- GAGACGGAGTCTCGCTCTGTC-3′) (75) with TB Green Premix Ex Taq II (TaKaRa) on Thermal Cycler Dice Real Time System II (TaKaRa) ...
-
bioRxiv - Cell Biology 2024Quote: RNA sequencing libraries of rRNA-depleted nuclear RNA or polyA RNA were prepared with SMARTer Stranded Total RNA-Seq Kit v2 (Takara) according to the manufacturer’s protocols ...
-
bioRxiv - Cell Biology 2024Quote: A mix of 4 μg of pcDNA3.1 plasmid containing the mouse Wnt10b cDNA (provided by Dr Bernardi, INRA, Montpellier) and 1 μg of pAcGFP-Nuc that encodes a nuclear GFP (Clontech) in 0.9% NaCl was electrotransfected directly into TA muscles of anesthetized ob/+ and ob/ob mice according to the protocol described by Grifone et al.[29] ...
-
bioRxiv - Plant Biology 2021Quote: ... we used the nuclear system Y2H mating standard operating procedure method as described in the manufacturer’s instructions (Clontech Laboratories, Inc., USA) to screen the protein that interacts with TiAP1 and further verified the obtained interaction protein ...
-
bioRxiv - Genetics 2021Quote: ... AD-MSCs and iPSCs by using the Genomic DNA Extraction Kit (TaKaRa). PCR was performed by using Q5 High-Fidelity DNA Polymerase (New England Biolabs ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR product was purified using MiniBEST agarose gel DNA extraction kit (TaKaRa). Following DpnI digestion of the template at room temperature overnight ...
-
bioRxiv - Cancer Biology 2022Quote: ... Total RNA was isolated from cells using the RNA extraction reagent (Takara). Reverse transcription was performed using PrimeScriptTM RT reagent kit (Takara) ...
-
bioRxiv - Developmental Biology 2022Quote: Total RNA extraction and reverse transcription were performed using NucleoSpin (Takara, #U955C) or Picopure (Thermo ...
-
bioRxiv - Plant Biology 2023Quote: ... RNA was extracted using TaKaRa MiniBEST Plant RNA Extraction Kit (TaKaRa, 9769) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: Total RNA was extracted by using MiniBEST Universal RNA extraction Kit (TaKaRa) according to the manufacturer’s instructions and quantified by NanoDrop instruments (ThermoFisher) ...
-
bioRxiv - Microbiology 2024Quote: ... The band was extracted and purified with Nucleospin Gel Extraction Kit (Takara). The gel-purified DNA was then further purified with Ampure XP beads (Beckman Coulter ...
-
bioRxiv - Molecular Biology 2024Quote: ... Viruses were extracted and purified by using an AAV extraction kit (Takara).
-
bioRxiv - Neuroscience 2022Quote: Between 1-3 ng of the nuclear RNA was used in the SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, cat # 634888) according to the manufacturer’s protocols ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Highly pure genomic DNA extraction was performed with NucleoSpin Tissue kit (Clontech Takara), according to the instructions of the manufacturer ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Highly pure genomic DNA extraction was performed with NucleoSpin Tissue kit (Clontech Takara), according to the instructions of the manufacturer ...