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Citations for Takara Bio :
1 - 50 of 1168 citations for Mouse ZNF583 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... in 35mm plates with 0.5 μg of pLKO shRNA or pLVX expression plasmids (Takara), 0.35 μg pCMV-dR8.2 and 0.15 μg pCMV-VSVG ...
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bioRxiv - Microbiology 2023Quote: ... pSIREN-shRNA-LucIRR and pSIREN-shRNA GAPDHHB were purchased from Clontech. pCDNA3.1 was purchased from Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were transfected with 1.0 μg of scrambled or Rai1-shRNA-expressing plasmids with the CalPhos Transfection kit (ClonTech) or Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... we expressed shRNA using SIREN-RetroQ (Clontech) gammaretroviral vector containing shRNA sequence targeting human TRIM5(Stremlau et al. ...
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bioRxiv - Developmental Biology 2024Quote: Lentiviral particles were produced following transient transfection of the shRNA-pLKO.1 vector and packaging plasmids into Lenti-X cells (632180; Takara Bio USA) using Attractene (301005 ...
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bioRxiv - Cell Biology 2023Quote: shRNA constructs were engineered on a pSIREN retroviral vector (Clontech). To deplete the endogenous expression of BIN1 ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviruses for shRNA transduction were generated in HEK293T cells (Takara). 8.4 µg of the vector of interest ...
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bioRxiv - Genetics 2022Quote: ... Lentiviral shRNA particles were prepared with Lenti-X 293T cells (Takara) and MISSION pLKO.1 plasmids (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... mouse DPPA3 cDNA was sub-cloned into a pGEX4T-3 plasmid using In-Fusion (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... transduced with concentrated shRNA-expressing lentiviruses harvested from LentiX-293T cells (Takara Bio; 632180), and cultured in BD Matrigel Matrix High Concentration (BD Biosciences ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... Sense and antisense oligonucleotides were annealed and inserted into the pLVX-shRNA vector (Clontech) at BamHI/EcoRI site ...
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bioRxiv - Cell Biology 2022Quote: ... A cassette containing the pre-shRNA sequence was inserted into pBAsi-mU6 (Takara Bio). The target sequences of each shRNA are as follows ...
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bioRxiv - Cancer Biology 2023Quote: Oligonucleotides were synthesized and cloned into the pSIREN-RetroQ retroviral shRNA expression vector (Clontech). Then pSIREN-RetroQ-Sirt7 and the negative control vector (pSIREN-RetroQ ...
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bioRxiv - Biophysics 2021Quote: Full length mouse ILK cDNA was cloned into the EcoRI site of pEGFP-N1 plasmid (Clontech). The R255A ...
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bioRxiv - Developmental Biology 2021Quote: The short hairpin RNA (shRNA)-expressing lentivirus vectors were constructed using pLVX-shRNA1 vector (Clontech). PRMT1-shRNA_#3–targeting sequence is GTGTTCCAGTATCTCTGATTA ...
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bioRxiv - Neuroscience 2020Quote: ... or cotransfected with IQSEC3 KD and shRNA-resistant myc-IQSEC3 using a CalPhos Transfection Kit (Takara) at DIV8 and immunostained at DIV14 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... purified plasmids with NucleoSpin Plasmid EasyPure (TaKaRa), and sequenced one plasmid for each NSP and MA gene from each of the 218 larval samples ...
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bioRxiv - Cancer Biology 2021Quote: shRNA directed against human MAGI1 and AMOTL2 were constructed and cloned in the pSIREN-RetroQ vector (TaKaRa) according to the manufacturer’s conditions between BamHI and EcoRI cloning sites as previously described for other genes 75 ...
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bioRxiv - Immunology 2022Quote: ... Retrovirus vectors carrying shRNA sequences were constructed by inserting annealed oligonucleotides into a pSIN-siU6 vector (Takara) between BamHI and ClaI sites ...
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bioRxiv - Cancer Biology 2022Quote: CPT1a knockdown cell lines were generated using the shRNA-expressing lentiviral pLKO-shRNA2 vector (No. PT4052-5; Clontech), with a puromycin selection cassette ...
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bioRxiv - Neuroscience 2023Quote: The Hes5.3-GFP plasmid derive from pEGFP-C1 plasmid (Clontech). The fragment bounded by the AseI and NheI restriction sites was deleted from pEGFP-C1 and the upstream sequences of Hes5.3 (1960 bp in length ...
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bioRxiv - Cancer Biology 2019Quote: Knockdown vectors expressing shRNAs were constructed by subcloning an annealed oligonucleotide into the pBAsi-hU6-Pur vector (TaKaRa Bio). Oligonucleotide sequences encoding the shRNA hairpin are shown below.
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bioRxiv - Neuroscience 2020Quote: Bacterial expression plasmids encoding mouse and human WT-α-synuclein in the inducible pRK172 backbone were transformed into BL21-CodonPlus (DE3) cells (Clontech). Cell pellets were lysed in 0.75M NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... eGFP plasmid (Clontech) at 5 ng/μL and pOCTmCherry at 1 μg/mL (Tradewell et al ...
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bioRxiv - Genomics 2022Quote: ... Individual colonies had their plasmids extracted using NucleoSpin Plasmid (Takara Bio) and Sanger sequenced to verify successful ligation with no sequence errors ...
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bioRxiv - Cell Biology 2020Quote: All GFP plasmids used were cloned into pEGFP-N1 plasmid (Clontech): pEGFP-N1 ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were transfected with control (scramble) or NUP153-specific shRNA vectors along with FLAG-hNUP153 expression vector using Xfect transfection reagent (Clontech) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... including a puromycin resistance cassette and a tet-responsive element for expression of shRNAs was established according to a publicly available protocol (46) using In-Fusion HD Cloning Kit (Clontech) (Supplementary Table 3) ...
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bioRxiv - Cell Biology 2021Quote: ... Expression of shRNA targeting and knocking-down Eklf was induced by the addition of 2 µg/ml of doxycycline (Clontech) for 96 hr ...
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bioRxiv - Developmental Biology 2023Quote: ... The constructed plasmids then were purified using nucleospin plasmid-transfection grade (TAKARA) and transfected into piezo1-deficient N2A cells (Sugisawa et al. ...
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bioRxiv - Genetics 2019Quote: ... Plasmid pEGFP-N1 (Clontech), expressing EGFP under the control of a CMV promoter ...
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bioRxiv - Developmental Biology 2021Quote: ... in plasmid pGBKT7 (Clontech), and as a prey to the activation domain of the GAL4 transcription factor (GAL4-AD ...
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bioRxiv - Neuroscience 2022Quote: ... and pHelper plasmid (TAKARA). The supernatants were purified and concentrated in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... pHelper plasmid (Takara Bio) and AAV transfer plasmids using TransIT-VirusGEN Transfection reagent (Mirus) ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid pEYFP-N1 (Clontech) was used as control ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: The shRNA target sequence for human HSF1 (NM_005526.4) was selected using the RNAi Target Sequence Selector (Clontech, Mountain View, CA, USA). The target sequences were ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was isolated using the FastGene Plasmid Mini Kit (Nippon Genetics, Tokyo, Japan) or NucleoSpin Plasmid EasyPure Kit (Takara Bio). The REase NdeI was employed for the linearization of plasmid DNAs ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was carried out to evaluate the syndecan-4 expression between lenti-synd4 shRNA and lenti-null group with SYBP Premix Ex Taq TM from Takara (Shiga, Japan) following the manufacturer’s instructions ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... Genes encoding the human MDM2 and pVHL30 proteins were obtained from commercial plasmid provided by GenScript (GenEZ plasmid OHu28568 and OHu23297) and cDNA transferred into pGBKT7 and pGADT7 plasmids (Clontech) to perform yeast two-hybrid assays (Y2H) ...
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bioRxiv - Cell Biology 2022Quote: ... pLVX-IRES-Puro plasmid (Clontech) was digested with AgeI and NotI (FastDigest ...
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bioRxiv - Neuroscience 2019Quote: Plasmid dsRed2-mito7 (Clontech #55838) was cut using NheI and NotI enzymes and then treated with DNA polymerase I Large Klenow fragment to isolate mito-dsRed2 cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... pGADT7 and pGBKT7 plasmids (Clontech) were transformed ...
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bioRxiv - Bioengineering 2023Quote: ... pHelper plasmid (Takara Bio Inc.), the plasmid expressing Rep and serotype 8 capsid (AAV8) ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids were extracted from yeast cells by Easy Yeast Plasmid Isolation Kit (#630467, Takara Bio) and transformed into E ...
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bioRxiv - Cancer Biology 2021Quote: ... plasmid or the enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1, Clontech/Takara Bio Europe). Transfections were achieved using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... plasmid or the enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1, Clontech/Takara Bio Europe). Transfections were achieved using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... transfections also contained 0.2 μg of the pEGFP plasmid (Enhanced Green Fluorescent Protein plasmid, Clontech), and EGFP expression served as a marker of transfection ...
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bioRxiv - Neuroscience 2020Quote: ... plasmids were rescued from yeast using the Easy Yeast Plasmid Isolation Kit (Clontech Takara Bio). Protein interactions were confirmed by co-transformation of the Nwd1 bait with each candidate prey plasmid into Y2H Gold yeast host cells ...
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bioRxiv - Neuroscience 2020Quote: ... plasmids were rescued from yeast using the Easy Yeast Plasmid Isolation Kit (Clontech Takara Bio). Protein interactions were confirmed by co-transformation of the Nwd1 bait with each candidate prey plasmid into Y2H Gold yeast host cells ...
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bioRxiv - Microbiology 2022Quote: ... Lentiviral plasmids were packaged into pseudoparticles via triple-plasmid co-transfection into HEK293T cells (TaKaRa). pTsin or pWPI plasmids ...