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Citations for Takara Bio :
1 - 50 of 304 citations for L Leucine N T Boc H2O 13C6 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... which was then cloned into the Aor51HI site in pUGW2 35S 97 using the In-Fusion cloning kit (TaKaRa Bio). The 2.5-kb HindIII-SacI fragment in the resulting plasmid ...
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bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex ...
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bioRxiv - Plant Biology 2023Quote: ... and selected on DDO (Synthetic Dropout Medium/-Tryptophan-Leucine) and TDO (Synthetic Dropout Medium/-Tryptophan-Histone-Leucine) media (Clontech) with corresponding concentration of 3-AT ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... transformants containing the desired plasmids were screened on a selective dropout (SD) medium lacking tryptophan (W) and leucine (L) (Clontech). Protein interactions were assessed on SD selection medium lacking LW ...
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bioRxiv - Plant Biology 2023Quote: ... were used to co-transform yeast strain AH109 and colonies carrying both vectors were selected on SD medium without tryptophan (-W) and leucine (-L) (TaKaRa 630317) at 28°C ...
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bioRxiv - Plant Biology 2024Quote: ... The clones growing in the –L/-T liquid selection medium (Clontech) were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech ...
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bioRxiv - Plant Biology 2022Quote: ... Transformants were selected on Synthetic Defined (SD) media lacking Leucine and Tryptophane (Clontech). Three individual clones were grown ON in liquid cultures (lacking Leucine and Tryptophane ...
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bioRxiv - Plant Biology 2024Quote: ... were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech) to determine interactions between the two proteins.
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bioRxiv - Genetics 2022Quote: ... Colonies were grown on media lacking histidine and leucine (DO Supplement -His/-Leu, Takara Bio) to select for the presence of both vectors ...
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bioRxiv - Biochemistry 2020Quote: ... pGAD plasmids were transformed into PJ49-4a yeast and were selected on leucine-dropout medium (Clontech, #630414). pTEF or pTEFdual plasmids were included to express proteins in trans and were transformed with pOBD plasmids and selected on media lacking tryptophan and uracil (Sunrise Science Products ...
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bioRxiv - Cancer Biology 2019Quote: ... then subcloned into T-clone vector pMD19-T (TaKaRa) to generate pT-DMP-2 ...
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bioRxiv - Cancer Biology 2019Quote: ... then subcloned into T-clone vector pMD19-T (TaKaRa) to generate pT-DMP-1 ...
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bioRxiv - Cell Biology 2019Quote: ... Unincorporated UTPs were removed using CHROMA SPIN-100-DEPC-H2O columns (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... the pSTT91 and pGAD424 plasmids cloned with potential interactors were introduced into the Saccharomyces cerevisiae strain L40 and grown for 2 days at 30°C on a leucine- and tryptophan- deficient synthetic defined premixed yeast growth medium (SD-Leu-Trp, TaKaRa Clontech)(Hollenberg et al. ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The Northern dot blots containing cDNAs from primary human tumors (T) with paired adjacent normal tissues (N) and cancer cell lines were obtained from Clontech (Cancer Profiling Array, #7840-1). The blots contained normalized cDNA isolated from tumors and the corresponding adjacent normal tissues from individual cancer patients ...
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bioRxiv - Immunology 2022Quote: ... pmCherry-N’ (Clontech) between NheI and HindIII restriction sites ...
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bioRxiv - Physiology 2021Quote: ... the pSTT91 and pGAD424 plasmids cloned with potential interactors were introduced into the Saccharomyces cerevisiae strain L40 and grown for 2 days at 30°C on a leucine- and tryptophan- deficient synthetic defined premixed yeast growth medium (SD-Leu-Trp, TaKaRa Clontech)(Hollenberg et al. ...
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bioRxiv - Genetics 2022Quote: ... the ptetO7-ALA1 strain was transformed with a vector (pAG425) to express wild-type or mutant AARS1 and grown on media lacking leucine (DO Supplement -Leu, Takara Bio). One colony was picked ...
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bioRxiv - Biochemistry 2020Quote: ... U-[15N,12C,2H]-labelled REC3 domain was overexpressed in BL21(DE3) cells containing chaperone plasmid pG-KJE8 (TAKARA, 3340) in M9 medium in 2H2O containing 2 g l−1 2H12C glucose (Sigma #552003 ...
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bioRxiv - Cell Biology 2020Quote: Lenti-X 293 T (Clontech), Neuro2A (mouse neuroblastoma ...
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bioRxiv - Cell Biology 2022Quote: ... and oligo(T)18 primers (Takara). Relative transcript amounts were measured by SYBR-green quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into pMD18-T (Takara) vector and then transformed into E.coli DH5α for sequencing ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... and oligo(T)18 primers (Takara). ARL3 cDNA and cDNAs encoding the six BBS proteins BBS1 ...
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bioRxiv - Microbiology 2021Quote: ... and inserted to pMD19-T (TAKARA) to obtain pMD19-T-MMP0852/0853 (Table S1) ...
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bioRxiv - Microbiology 2019Quote: ... and inserted to pMD19-T (TAKARA) to obtain pMD19-T-Pmcr-tetR-Tmcr (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2020Quote: ... the amplified PCR products were cloned into a T/A cloning vector PMD18-T (TaKaRa, Tokyo, Japan), and later excised from PMD18-T plasmid using NotI and XhoI restriction sites ...
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bioRxiv - Physiology 2020Quote: ... N-glycosidase F (PNGase, Takara, 4450) was used as previously reported [22] ...
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bioRxiv - Cancer Biology 2020Quote: ... was cloned into pMD19-T simple (TAKARA) to obtain pMD19-T-DMP firstly ...
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bioRxiv - Microbiology 2021Quote: ... Lenti-X 293 T cells (Takara Bio) grown in 10 cm dish were transiently transfected with the following plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... cloned into T-Vector pMD20 (Takara, 3270), and sequenced (Thermo ...
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bioRxiv - Systems Biology 2020Quote: ... or pCMV-HA-N vector (Clontech #635690), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... and cloned into pmRFP-N vectors (Clontech). A construct coding for N-terminally GFP-tagged full-length rat Shank3 in the pHAGE vector was obtained from Alex Shcheglovitov (University of Utah) ...
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bioRxiv - Cell Biology 2021Quote: ... and inserted into T-Vector pMD20 (Takara Bio) using DNA Ligation Kit
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into a pMD19-T vector (Takara). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... and cloned into pMD18-T vector (TaKaRa, China) and the recombinant plasmids were picked and sent to Tsingke Biotechnology Co. ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were transferred to retronectin-coated (Takara) plates previously spinoculated with retroviral particles at 2000ξg for 1.5h ...
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bioRxiv - Plant Biology 2023Quote: ... 0.05% T-20 and protease inhibitor cocktail (Takara)] ...
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bioRxiv - Plant Biology 2024Quote: ... The pMD18-T vector was procured from TaKaRa Bioengineering (Dalian ...
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bioRxiv - Plant Biology 2019Quote: ... and the Gal4 AD fused with SV40 large T-antigen (pGADT7-T) from the Matchmaker Gold Yeast Two-Hybrid System kit (Takara Bio) were used as positive control ...
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bioRxiv - Cancer Biology 2023Quote: ... The H2afx coding sequence from Mus musculus was ordered from Eurofins and cloned into the pOZ-N-FH backbone adding the 1xHA tag at the N terminus using the in-fusion HD cloning kit (#12141, Takara). Full length wild type H2afx coding sequence was then mutagenized to obtain the desired S139A point mutation.
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bioRxiv - Biophysics 2021Quote: ... RNA was synthetized using the T7-Scribe transcription kit (Cellscript) and purified with Chroma Spin DEPC-H2O columns (Clontech, CA, USA). The ncAA naphthalene was ligated to the tRNA with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... the supernatant was removed and the pellet was dissolved in 20 ul RNase-free H2O (Takara Bio Inc., Otsu, Shiga, Japan). Quantitation of the RNAs was performed spectrophotometrically at 260 nm ...
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bioRxiv - Microbiology 2019Quote: ... The amplified fragments were cloned into pMD18-T (TaKaRa), confirmed by sequence analysis ...
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bioRxiv - Immunology 2019Quote: ... Connected DNA fragment to pMDTM18-T vector (TaKaRa 6011) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... The cloning vector pMD20-T was purchased from Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into T-vector pMD20 (Takara Bio Inc.). To amplify Vero-PVR1 ...
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bioRxiv - Neuroscience 2022Quote: ... and the AAVpro 293 T cell line (#632273, Takara) following the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: mRNA was captured using poly-T coated dishes (Clontech) and used for library construction using SMARTer technology (SMARTer Stranded RNA-seq Kit ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA was captured using poly-T coated dishes (Clontech) and SMARTer technology was used for library construction (SMARTer Stranded RNA-seq Kit).
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bioRxiv - Cell Biology 2023Quote: ... Clone and sequence using the pMD18-T vector (TaKara).