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Citations for Takara Bio :
1 - 50 of 787 citations for L Alanine N T Boc 2 13C 98 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Doxycycline (2 mg/L, Clontech) was also included on d0 to induce TetO gene expression by binding to rtTA and the TetO promoter upstream of the Ngn2 gene ...
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bioRxiv - Plant Biology 2024Quote: ... The clones growing in the –L/-T liquid selection medium (Clontech) were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech ...
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bioRxiv - Plant Biology 2024Quote: ... were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech) to determine interactions between the two proteins.
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bioRxiv - Microbiology 2019Quote: ... Specific PCR products were excised from 2% agarose gel and cloned into pMD19-T (TaKaRa) and then sequenced ...
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bioRxiv - Microbiology 2021Quote: ... Specific PCR products were excised from 2% agarose gel and cloned into pMD19-T (TaKaRa) and then sequenced ...
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bioRxiv - Cancer Biology 2019Quote: ... then subcloned into T-clone vector pMD19-T (TaKaRa) to generate pT-DMP-2 ...
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bioRxiv - Cancer Biology 2019Quote: ... then subcloned into T-clone vector pMD19-T (TaKaRa) to generate pT-DMP-1 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The Northern dot blots containing cDNAs from primary human tumors (T) with paired adjacent normal tissues (N) and cancer cell lines were obtained from Clontech (Cancer Profiling Array, #7840-1). The blots contained normalized cDNA isolated from tumors and the corresponding adjacent normal tissues from individual cancer patients ...
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bioRxiv - Immunology 2022Quote: ... pmCherry-N’ (Clontech) between NheI and HindIII restriction sites ...
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bioRxiv - Cell Biology 2021Quote: pAAVS1-CMV-HA-TRF2 and variants encoding alanine-substitution mutants of TRF2 (used in Figure 1E-G) were prepared using the In-Fusion (Clontech) reaction ...
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bioRxiv - Microbiology 2020Quote: ... The EGFP-nsP3 truncations and alanine substitution mutations were constructed by subcloning the corresponding nsP3-encoding PCR products into the vector pEGFP-C1 (Clontech) using XhoI-KpnI restriction sites.
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bioRxiv - Molecular Biology 2021Quote: ... two to three amino acids in GFP-SP-BAP were replaced by alanine for each construct using the CloneAmp HiFi PCR Premix (Takara Bio) according to the manufacturer.
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bioRxiv - Cell Biology 2023Quote: ... and N-SREBP2 (1440 bps) were amplified using high-fidelity PCR (Advantage-HF 2 PCR kit, #639123; Clontech) from human pcDNA3.1-2xFLAG-SREBP1a (#26801 ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated for 1 h at room temperature with Odyssey blocking buffer and 0.2 % PBS-T (PBS containing 0.2 % (v/v) Tween-20) with rat anti-HA clone 3F primary antibody (Clontech) at 1:1,000 dilution ...
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bioRxiv - Genetics 2019Quote: BAC clone (BAC PAC RPCI- 98 library) DNA was extracted using a MIDI-prep kit (Clontech #740410). The probe for the bw locus was Clone BACR48M01 ...
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Relationship between True Digestibility of dietary Phosphorus and Gastrointestinal Bacteria of GoatsbioRxiv - Microbiology 2019Quote: ... Taq buffer 5 μL of 10×Ex (20 mmol/L Mg 2+;TaKaRa Inc., Dalian, China), template DNA 0.35 μg ...
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bioRxiv - Cell Biology 2020Quote: Lenti-X 293 T (Clontech), Neuro2A (mouse neuroblastoma ...
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bioRxiv - Immunology 2024Quote: ... T cells were activated for 2 days and NK cells were activated for 4 days followed by retronectin (Takara, T100B) mediated viral transduction ...
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bioRxiv - Pathology 2021Quote: ... Expression of the GST-N protein of SARS-CoV-2 was induced by isopropyl-D-1-thiogalactopyranoside (0.3 mM IPTG, Takara Bio). The cell pellets were sonicated ...
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bioRxiv - Synthetic Biology 2023Quote: ... T cells were incubated at 3×104 cells/well in 96-well U bottom plates for 24 hours in T cell media containing 50 IU/mL rhIL-2 and varying concentrations of AP20187 (B/B homodimerizer, Takara, 635058). After 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and oligo(T)18 primers (Takara). Relative transcript amounts were measured by SYBR-green quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into pMD18-T (Takara) vector and then transformed into E.coli DH5α for sequencing ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... and oligo(T)18 primers (Takara). ARL3 cDNA and cDNAs encoding the six BBS proteins BBS1 ...
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bioRxiv - Microbiology 2021Quote: ... and inserted to pMD19-T (TAKARA) to obtain pMD19-T-MMP0852/0853 (Table S1) ...
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bioRxiv - Microbiology 2019Quote: ... and inserted to pMD19-T (TAKARA) to obtain pMD19-T-Pmcr-tetR-Tmcr (Supplementary Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... real-time RT-PCR was performed with SARS-CoV-2 N primer sets and SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler Nano (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... the amplified PCR products were cloned into a T/A cloning vector PMD18-T (TaKaRa, Tokyo, Japan), and later excised from PMD18-T plasmid using NotI and XhoI restriction sites ...
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bioRxiv - Physiology 2020Quote: ... N-glycosidase F (PNGase, Takara, 4450) was used as previously reported [22] ...
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bioRxiv - Cancer Biology 2023Quote: ... lentiviral constructs pCMV-VSV-G and pCMV-dR8.2 dvpr 98 were transfected in HEK293T cells by the calcium-phosphate method (CalPhos Mammalian Transfection Kit, Takara, 631312) for virus production ...
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bioRxiv - Microbiology 2023Quote: ... The N-terminal 3xFLAG-tagged ORF67.5 expression plasmid was constructed using the insert extracted from a previously constructed N-terminal 2×S-tagged ORF67.5 expression plasmid digested with EcoRI and SalI (Takara Bio, Shiga, Japan) (32) ...
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bioRxiv - Cancer Biology 2020Quote: ... was cloned into pMD19-T simple (TAKARA) to obtain pMD19-T-DMP firstly ...
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bioRxiv - Microbiology 2021Quote: ... Lenti-X 293 T cells (Takara Bio) grown in 10 cm dish were transiently transfected with the following plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... cloned into T-Vector pMD20 (Takara, 3270), and sequenced (Thermo ...
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bioRxiv - Systems Biology 2020Quote: ... or pCMV-HA-N vector (Clontech #635690), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... and cloned into pmRFP-N vectors (Clontech). A construct coding for N-terminally GFP-tagged full-length rat Shank3 in the pHAGE vector was obtained from Alex Shcheglovitov (University of Utah) ...
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bioRxiv - Cell Biology 2021Quote: ... and inserted into T-Vector pMD20 (Takara Bio) using DNA Ligation Kit
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into a pMD19-T vector (Takara). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... and cloned into pMD18-T vector (TaKaRa, China) and the recombinant plasmids were picked and sent to Tsingke Biotechnology Co. ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were transferred to retronectin-coated (Takara) plates previously spinoculated with retroviral particles at 2000ξg for 1.5h ...
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bioRxiv - Plant Biology 2023Quote: ... 0.05% T-20 and protease inhibitor cocktail (Takara)] ...
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bioRxiv - Plant Biology 2024Quote: ... The pMD18-T vector was procured from TaKaRa Bioengineering (Dalian ...
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bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex ...
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bioRxiv - Plant Biology 2019Quote: ... and the Gal4 AD fused with SV40 large T-antigen (pGADT7-T) from the Matchmaker Gold Yeast Two-Hybrid System kit (Takara Bio) were used as positive control ...
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bioRxiv - Cancer Biology 2023Quote: ... The H2afx coding sequence from Mus musculus was ordered from Eurofins and cloned into the pOZ-N-FH backbone adding the 1xHA tag at the N terminus using the in-fusion HD cloning kit (#12141, Takara). Full length wild type H2afx coding sequence was then mutagenized to obtain the desired S139A point mutation.
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bioRxiv - Cell Biology 2022Quote: ... while pGADT7-CmVPS41s and pGADT7-T control prey plasmids were transformed into yeast strain Y187 following the Yeastmaker Yeast Transformation System 2 instructions (Clontech by Takara Bio, Mountain View, USA). The transformed cells were grown either in SD-Trp agar plates for Y2HGold or in SD-Leu agar plates for Y187 at 30 ºC for 3-5 days ...
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bioRxiv - Microbiology 2019Quote: ... The amplified fragments were cloned into pMD18-T (TaKaRa), confirmed by sequence analysis ...
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bioRxiv - Immunology 2019Quote: ... Connected DNA fragment to pMDTM18-T vector (TaKaRa 6011) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... The cloning vector pMD20-T was purchased from Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into T-vector pMD20 (Takara Bio Inc.). To amplify Vero-PVR1 ...
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bioRxiv - Neuroscience 2022Quote: ... and the AAVpro 293 T cell line (#632273, Takara) following the manufacturers’ instructions ...