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Citations for Takara Bio :
1 - 50 of 136 citations for Kinase Domain of Pyruvate Kinase liver Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and T4 polynucleotide kinase (Takara). The extension reaction was performed with the AccuPower® DNA Sequencing Kit (Bioneer) ...
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bioRxiv - Cell Biology 2022Quote: ... phosphorylated by T4 polynucleotide kinase (TaKaRa) and inserted between SpeI and NotI restriction sites (pFastBac1-hCAP-D3-[3C]-StrepII ...
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bioRxiv - Cell Biology 2022Quote: ... phosphorylated by T4 polynucleotide kinase (TaKaRa), and inserted between BamHI and EcoRI restriction sites by using restriction enzymes and a DNA ligation kit (TaKaRa) ...
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bioRxiv - Genomics 2019Quote: ... together with phosphorylated (T4 polynucleotide kinase, Takara) primers that spanned the length of the RNA of the retrozyme (i.e. ...
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bioRxiv - Cell Biology 2021Quote: ... and then phosphorylated by T4 polynucleotide kinase (Takara, 2021S). pX459 was digested with BbsI ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by conducting a T4 polynucleotide kinase reaction (TAKARA). After phosphorylation ...
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bioRxiv - Genetics 2020Quote: ... 5′-phosphorylated by T4 polynucleotide kinase (TaKaRa, Shiga, Japan), self-ligated by T4 ligase (TaKaRa ...
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bioRxiv - Plant Biology 2023Quote: ... and end-labeled by T4 polynucleotide kinase (Takara Bio) and [γ-32P]-ATP (PerkinElmer) ...
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bioRxiv - Plant Biology 2021Quote: ... Strands with a 5′ monophosphate were radiolabeled with T4 polynucleotide kinase (Takara) and [γ-32P]ATP ...
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bioRxiv - Microbiology 2019Quote: ... and a Renilla luciferase driven by the thymidine kinase promoter (pRLTK; Clontech) was performed according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... a stepwise RNA end repair was carried out using T4 polynucleotide kinase (TaKaRa). A 3′-dephosphorylation and 5′-phosphorylation reaction was performed using T4 PNK enzyme (TaKaRa) ...
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bioRxiv - Biochemistry 2021Quote: ... T4 polynucleotide kinase and TransIT®-LT1 Reagent were purchased from TAKARA (Shiga, Japan). SB203580 was purchased from Adipogen Life Science (San Diego ...
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bioRxiv - Cell Biology 2023Quote: ... and the 5′-end was phosphorylated with T4 polynucleotide kinase (Cat. # 2021S: Takara, Kyoto, Japan). This phosphorylated DNA fragment was ligated to the Bbs I cloning site in pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Microbiology 2023Quote: ... corresponding to these sgRNA sequences (LpAsp_For and LpAsp_Rev as well as LpCht_For and LpCht_Rev) were phosphorylated by T4 polynucleotide kinase (TAKARA) followed by annealing and cloning into BbsI digested pSPneogRNAH vector (Zhang and Matlashewski ...
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bioRxiv - Biochemistry 2022Quote: ... An oligonucleotide probe against snoRNA58 labeled with [γ-32P] ATP by T4 polynucleotide kinase (Takara, cat# 2021A) was used as a loading control ...
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bioRxiv - Microbiology 2020Quote: The VZV thymidine kinase gene (ORF36) was inserted into the Lenti-X pLVX-Puro vector (Clontech, Mountain View, CA) with XhoI and EcoR I sites ...
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bioRxiv - Microbiology 2023Quote: ... inverse PCR amplification was conducted with oligonucleotides and templates listed in Table S1 and the resulting PCR products were treated with T4 Polynucleotide Kinase (Takara) and ligated with a DNA ligation kit (Takara) ...
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bioRxiv - Molecular Biology 2023Quote: ... synthesized 5’ and 3’ fragments of the target RNA were 32P-radiolabeled at the 5’ end using T4 polynucleotide kinase (Takara). After gel purification ...
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bioRxiv - Microbiology 2020Quote: ... ramulus DSM 15684 were cloned without signal peptide or transmembrane domain from genomic DNA using In-Fusion cloning (Takara) and the primers in Table S8 into the EcoRI and Ncol restriction sites of the corresponding plasmids ...
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bioRxiv - Plant Biology 2022Quote: ... The CWLP coding region without signal peptide (SP) was cloned in frame downstream of the GAL4 DNA-binding domain in the bait vector pGBKT7 (Clontech) and introduced into the yeast strain AH109 (Clontech) ...
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bioRxiv - Cell Biology 2024Quote: ... total mouse liver mRNA (Takara) was used.
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bioRxiv - Cell Biology 2023Quote: ... Gal4-activation domain (Gal4ad) vector pACT2 (Clontech) as described (Guo ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were cloned in plasmids derived from the 2 hybrid vectors pGADT7 (GAL4-activating domain) and pGBKT7 (GAL4-binding domain) creating N terminal fusions and transformed in yeast haploid strains Y187 and AH109 (Clontech), respectively ...
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bioRxiv - Biophysics 2022Quote: ... with insertion of N-terminal residues MATLEK a part of the N17 domain and C-terminal residues PQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRP which comprise the PR (proline-rich) domain containing the C38 domain using primers as a part of In-Fusion cloning protocol (Takara). eGFP-PolyQ31 was adapted from eGFP-PolyQ74 through the variability of Q-length PCR products amplified using CloneAmp HiFi PCR Premix (Takara) ...
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bioRxiv - Plant Biology 2022Quote: ... fragments were recombined into GW versions of the GAL4 DNA-binding domain vector pGBT-9 and the activation domain vector pGAD424 (Clontech). Oligonucleotides used for cloning are listed in Supplementary Table S3.
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bioRxiv - Cell Biology 2024Quote: ... where the entire ORF of the corresponding gene was inserted in frame into the pGADT7 (GAL4 activation domain) or pGBKT7 (GAL4 binding domain) plasmid (Clontech).
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bioRxiv - Plant Biology 2023Quote: ... and a rabbit LexA binding domain antibody (Clontech), respectively.
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bioRxiv - Plant Biology 2022Quote: ... Cytosolic domain fragments of NbPMA3 (F1, F2 or F3) were fused with the Gal4 activation domain in pGADT7 (Clontech, PT3249-5, USA) and inserted into the Y187 strain under selection with SD/-Leu ...
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bioRxiv - Developmental Biology 2022Quote: ... Each domain deletion PCR amplification was performed by TAKARA-HIFI Tag by following the manufactory guides ...
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bioRxiv - Genetics 2019Quote: ... liver and kidney), mouse tissues (brain, liver, heart, testis and kidney) and rat tissues (brain, liver and kidney) were purchased from Takara Bio Inc.
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bioRxiv - Molecular Biology 2022Quote: ... 1 U/μL ribonuclease inhibitor (porcine liver) (TaKaRa Bio), 0.25 U/μL AMV reverse transcriptase XL (TaKaRa Bio) ...
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bioRxiv - Cell Biology 2024Quote: ... Domain swapped constructs were generated by in-fusion cloning (Takara Bio). FZD(1-10)-V5-IRES-mKate constructs were a kind gift of Karl Willert (Department of Cellular & Molecular Medicine ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.2 U/μL of ribonuclease inhibitor (porcine liver) (TaKaRa Bio) in 44.5 μL of the RNase-free Buffer RDD was added to each sample ...
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bioRxiv - Microbiology 2022Quote: Human liver cDNA library was obtained from Clontech (California, USA). Dual Luciferase reporter assay kit and CellTiter96 Aqueous one solution cell proliferation assay kits were from Promega (Madison ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pooled total RNA samples from human liver were obtained from Clontech. Each sample (2 μg ...
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bioRxiv - Plant Biology 2020Quote: ... βC1 was fused with binding domain and cloned into pGBKT7 BD (Takara Bio). Plasmids were transformed into AH109 strain as described previously (Gietz and Woods ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA was isolated from liver of mice using RNAiso plus (TaKaRa). RNA was reverse-transcribed using the PrimeScript RT reagent Kit (TaKaRa) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pooled total RNA of human kidney and liver were purchased from Clontech. Total RNA (2 µg ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from human liver and fetal brain was purchased from Clontech Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 50mg of liver sample was taken in 1ml RNAiso (Takara Cat.-9108/9109) and RNA was isolated according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from liver organoids using RNAiso Plus reagent (TaKaRa, Japan) in combination with chloroform and isopropanol ...
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bioRxiv - Plant Biology 2020Quote: ... and Lamin (LAM) were cloned into the DNA-binding domain vector pGBKT7 (Clontech Matchmaker System). The full-length open reading frame of NAA50 ...
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bioRxiv - Plant Biology 2020Quote: All SUMO CDS were translationally fused with the activation domain of pGADT7 AD (Takara Bio). βC1 was fused with binding domain and cloned into pGBKT7 BD (Takara Bio) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Domain swapped HsPLZF was constructed by inverse PCR and the In-Fusion system (Takara Bio). Deletion mutation and amino acid mutation of each protein was performed by inverse PCR.
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from the liver tissue by using Trizol Reagent (Takara, Japan), and complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2023Quote: ... a region spanning the ENE domain was amplified by conventional PCR (PrimeStar HS mix, Takara Bio) with primer sequences listed in Table S9 ...
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bioRxiv - Genetics 2021Quote: The coding sequence of the MAR was inserted into the Gal4 DNA-binding domain vector pGBKT7 (Clontech); the coding sequences for full length Nup60 and Nup60(188-388 ...
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bioRxiv - Immunology 2022Quote: ... The NTD domain was purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Systems Biology 2023Quote: ... sSH2 domains were immediately purified by the N-terminal His6 tag with TALON® resin (Takara Bio) using a gravity column (detailed purification method in the Supporting Methods 1) ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA samples were isolated from the rat liver using RNAiso Plus (D9109; TaKaRa, Dalian,China). RNA samples were converted to cDNA using a RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...