Labshake search
Citations for Takara Bio :
1 - 50 of 378 citations for Integrin Alpha M CD11b ITGAM Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2021Quote: ... 2021: Escherichia coli DH5 alpha (Takara (Korea)) ...
-
bioRxiv - Cell Biology 2024Quote: ... 1999) with a retroviral vector expressing integrin β1 under a tet-inducible promotor (Retro-X™ Tet-On®; Clontech, USA). Integrin β1-induced and FACS sorted cells were cultured in DMEM with 10% fetal bovine serum ...
-
bioRxiv - Microbiology 2022Quote: ... X-alpha-Gal (X-α-gal) and aureobasidin A (AbA) were obtained from TaKaRa. Plasmids ...
-
Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threatsbioRxiv - Molecular Biology 2019Quote: ... DNA was transferred to HyBond N+ in transfer buffer (0.4 M NaOH, 1 M NaCl) and UV cross-linked before hybridization with a random primed probe (Takara) overnight at 42°C and washed twice for 20 min with 0.5× SSC 0.1% SDS at 65°C.
-
bioRxiv - Bioengineering 2024Quote: ... CD11b-specific amplicons were generated via 3-step nested PCR using PrimeSTAR GXL (Takara Bio, Kusatsu, Shiga, Japan) according to the manufacturer’s protocol with a 60°C annealing temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissues were rinsed four times for 15 min each with 0.1 M PBS and incubated with secondary antibodies anti-DsRed (1:500; RRID:AB_10013483; Living Colors DsRed polyclonal; Takara Bio USA, San Jose, CA) and anti-Troma1 (1:500 ...
-
bioRxiv - Cell Biology 2020Quote: ... M-MLV reverse transcriptase and RNase inhibitor (TaKaRa, D2639A). Q-PCR was performed in triplicate with Power SYBR® Green (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2019Quote: ... and retrotranscription was performed with M-MLV retrotranscriptase (Clontech). Subsequently ...
-
bioRxiv - Immunology 2024Quote: ... cDNA was subsequently synthesized using Reverse Transcriptase M-MLV (TakaRa) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... The extracted probes were radioactively labeled with 2.5 μl [alpha-32P] dCTP (Amersham) according to the instructions of the kit (LaddermanTM Labeling Kit, Takara). The probe was mixed with 100 μl TE buffer (10mM ...
-
bioRxiv - Molecular Biology 2022Quote: ... and digested with SacI and XbaI enzymes in M buffer (TaKaRa). The gene sequence included a SacI restriction site in the second intron and ...
-
bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription was performed using the M-MLV reverse system (Takara) to obtain cDNA ...
-
bioRxiv - Immunology 2020Quote: In-vivo and in-vitro generated CD11b+ Gr1+ MDSCs were sorted on flow cytometry machine and cells pelleted for RNA isolation using RNAiso plus (Takara bio. Inc., Japan). The extracted RNA was converted to cDNA with Takara kit (Takara bio ...
-
bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using M-MLV reverse transcriptase (TaKaRa Biotechnology, Dalian, China). In brief ...
-
bioRxiv - Immunology 2022Quote: ... and first strand cDNA was synthesized with Reverse Transcriptase M-MLV (TaKaRa). Real-time PCR was performed using Hieff® qPCR SYBR Green Master Mix (No Rox ...
-
bioRxiv - Developmental Biology 2020Quote: ... Reverse transcription was performed by using reverse transcriptase M-MLV (TaKaRa, Japan) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... was subcloned into the lentiviral expression vector pLVX-M-puro (Takara 632164). For CRISPR-KO experiments ...
-
bioRxiv - Biochemistry 2019Quote: ... CoAA and hnRNP M fragments were inserted in-frame into pM vector (Clontech) containing Gal4 DNA-binding domain to produce Gal4-fusion proteins ...
-
bioRxiv - Microbiology 2021Quote: ... followed by cDNA synthesis using M-MLV reverse transcriptase (TaKaRa Biotechnology, Dalian, China). The qualified cDNAs were subjected to high-throughput sequencing for viral metagenomic analysis by the Beijing Genome Institute (BGI ...
-
bioRxiv - Molecular Biology 2020Quote: ... The plug was equilibrated twice in 1 ml of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
-
bioRxiv - Plant Biology 2022Quote: ... cDNA synthesis was performed using the M-MLV reverse transcriptase (Takara Bio, Inc.) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
-
bioRxiv - Developmental Biology 2021Quote: ... and reverse-transcribed using random hexamer-primers and M-MuLV reverse transcriptase (Takara, Japan). The resulted cDNA was used to perform quantitative PCR in an Applied Biosystems Step One Plus real-time PCR system (Applied Biosystems ...
-
bioRxiv - Cell Biology 2020Quote: ... the reverse transcription reactions were performed using reverse Transcriptase M-MLV (RNase H-) (TaKaRa). The cDNA was then used for real-time qPCR using the SYBR Green qPCR Master Mix (Bi-make ...
-
bioRxiv - Genomics 2019Quote: ... 9.0 μL of 3 SMART™ CDS Primer II A (12 M, Clontech, 634936), and 1.4 μL of Loading Reagent (20X ...
-
bioRxiv - Microbiology 2024Quote: ... and cDNA was synthesized using the M-MLV Reverse Transcriptase cDNA Synthesis Kit (Takara) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2019Quote: ... The first strand cDNA was generated with M-MLV first strand cDNA synthesis kit (Takara), using oligo (dT ...
-
bioRxiv - Developmental Biology 2023Quote: ... 1 µg of total RNA was converted to cDNA using M-MLV reverse transcriptase (TAKARA) under standard conditions with oligo(dT ...
-
bioRxiv - Genomics 2023Quote: The nuclei containing about 1,000 ng of DNA were resuspended with 1x M buffer (Takara). To permeabilize nuclei ...
-
bioRxiv - Cell Biology 2021Quote: ... and purified under denaturing condition (8 M urea) using the TALON metal affinity resin from Clontech. Purified fusion proteins were used to produce antibodies in guinea pigs from Covance Inc ...
-
bioRxiv - Molecular Biology 2022Quote: ... EBIV M/L segment-specific sequences were amplified by PCR including Universal Primer A Mix (Takara), Gene-Specific Primers (GSPs ...
-
bioRxiv - Cell Biology 2021Quote: ... cDNAs were synthesized from total RNA using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (Takara, Japan) based on the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1× M buffer containing 160 units of NheI (TaKaRa) overnight at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... The S-F gene was inserted between the P and M gene of pNDV_LaSota (LS) L289A mutant (NDV_LS/L289A) antigenomic cDNA by in-Fusion cloning (Clontech). The recombination product was transformed into NEB® Stable Competent E ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was obtained from 1 µg of total RNA from each sample (Super M-MLV reverse transcriptase, TAKARA) and QPCR was performed using TaqMan™ Gene Expression Master Mix (4369016 ...
-
bioRxiv - Molecular Biology 2020Quote: ... the plug was incubated in 160 μl of 1× M buffer containing 160 units of Nhe I (TaKaRa) for 7 hr at 37°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1x M buffer containing 160 units of Nhe I (TaKaRa) overnight at 37°C ...
-
bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from M-exos and HM-exos using the Trizol reagent (Takara Biotechnology, Japan; 9109), and RNA integrity was assessed using a 2100 bioanalyzer (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 g RNA was used to synthesize the cDNA using reverse transcription M-MLV (RNase-free) kits (TaKaRa, Japan). qRT-PCR was performed with SYBR green PCR master mix (ToYoBo ...
-
bioRxiv - Immunology 2021Quote: ... The sequence of the HXP-S was inserted into pNDV_LS/L289A rescue plasmid (between P and M genes) by in-Fusion cloning (Clontech). The recombination product was transformed into MAX Efficiency™ Stbl2™ Competent Cells (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2022Quote: ... First strand cDNA synthesis was performed on mRNA fragments with SMARTscribe™ M-MLV Reverse Transcriptase (Takara Bio Inc.). Illumina barcode PCR reactions were performed to prepare the cDNA for Illumina 75 bp paired-end sequencing ...
-
bioRxiv - Microbiology 2024Quote: ... M-MLV reverse transcriptase (2641A) and TB Green® Fast qPCR Mix (RR430A) were purchased from Takara (Tokyo, Japan).
-
bioRxiv - Cancer Biology 2020Quote: ... The primary antibody rabbit anti-human Cas9 Polyclonal Antibody (Clontech) was diluted 1 ...
-
bioRxiv - Immunology 2019Quote: ... Rab 5 and Rab 7 cDNA (a gift of M. Sandor) and human CD81 cDNA (Open Biosystems) were cloned into pmCherry-N1 vector (Clontech). Raji/DC-SIGN cells were electroporated with 1 µg of indicated plasmid using the Eppendorf Multiporator in iso-osmolar electroporation buffer using a 90 µs ...
-
bioRxiv - Bioengineering 2019Quote: ... The vector harbored the URA3 and the leu2-d markers and the 2-m replication origin derived from the pYEX-S1 (Clontech) backbone ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins induced by 1 mM IPTG entered inclusion bodies and were extracted with 8 M urea and purified with TALON Metal Affinity Resin (Takara) under denaturing conditions ...
-
bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins corresponding to the full-neck regions of myosin VIIIs were solubilized using 8 M urea and purified using a Ni-NTA His60 column (Takara), or for GST-tandem-IQ fusions ...
-
bioRxiv - Microbiology 2022Quote: ... monoclonal antibody (Clontech) and peroxidase-labeled anti-mouse IgG (H + L ...
-
bioRxiv - Plant Biology 2020Quote: The total RNA of the buds collected for the transcriptome research was extracted by the EASYspin Plus Plant RNA Kit RN37 (Aidlab Biotechnology) and cDNA synthesis by the M-MLV RTase cDNA Synthesis Kit (TaKaRa Company). cDNA amplified fragment length polymorphism (cDNA-AFLP ...