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Citations for Takara Bio :
1 - 50 of 2147 citations for Indeno 1 2 3 Cd Pyrene D12 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... 9.0 μL of 3 SMART™ CDS Primer II A (12 M, Clontech, 634936), and 1.4 μL of Loading Reagent (20X ...
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bioRxiv - Molecular Biology 2023Quote: ... 14-3-3γ CDS was amplified by PCR and subcloned into pCMV-mCherry (Clontech). Deletion and point mutations of SMAUG1 were created using Q5 site-directed mutagenesis kit (NEB) ...
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bioRxiv - Microbiology 2023Quote: The pEGFP-C1 plasmids including AFF4 3’UTR sites “1-2-3” were generated using pEGFP-C1 (Clontech). The following complementary oligonucleotides were annealed in respective pairs as above (1.25 µM each in 75 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... The N-terminal 3X-FLAG-tagged ThPOK expression plasmid was generated by cloning the ThPOK coding DNA sequence (CDS) in the backbone of pEGFPC-3 (Clontech) plasmid after replacing the CDS of EGFP with 3X-FLAG ...
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bioRxiv - Cancer Biology 2024Quote: ... we employed the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) or WST-1 (Water-Soluble Tetrazolium 1) assay from Takara, Japan ...
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bioRxiv - Systems Biology 2022Quote: Following the transfer of samples into a 384-well plate containing RT-PCR buffer with 3’ SMART-Seq CDS Primer IIA (SMART-Seq® v4 PLUS Kit, TaKaRa, cat# R400753); the samples were immediately denatured at 72ºC for 3 min and chilled on ice for at least 2 min ...
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bioRxiv - Genomics 2020Quote: ... RNase Inhibitor (0.4 U) and 1.92 μM of the 3’ oligo dT terminating primer: SMART-Seq® ICELL8® CDS (Takara Bio USA, CA, USA).
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bioRxiv - Neuroscience 2020Quote: ... 341F (5’-CCTACGGGNGGCWGCAG-3’) and 805R (5’-GACTACHVGGGTATCTAATCC-3’) or (2) 341F (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’) and 806R (5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACHVGGGTWTCTAAT-3’) (TaKaRa Bio, Shiga, Japan). The second PCR was performed to add the index sequences for Illumina sequencing with barcode sequences ...
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bioRxiv - Plant Biology 2020Quote: ... The PuERF2 CDS was cloned into the pRI101 vector (TakaRa) to allow expression of PuERF2 as a fusion with green fluorescent protein (GFP ...
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bioRxiv - Developmental Biology 2020Quote: ... SlPP2C3 (Solyc06g076400.2.1) CDS was cloned into pRI101-AN plasmid (TAKARA). For SlPP2C3-RNAi tomato transformation ...
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bioRxiv - Plant Biology 2023Quote: ... The full-length and truncate CDS of TaNAM-A1a (residues 1–220) were cloned into bait vector pGBKT7 (Clontech) respectively to generate bait plasmids BD-NAM-A1full and BD-NAM-A11-220 ...
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bioRxiv - Biochemistry 2019Quote: ... coli MS115-1 (CD-NTase018 from (Whiteley et al., 2019)) were synthesized (GeneArt) and inserted into vectors pBridge and pGADT7 (Clontech) by isothermal assembly ...
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bioRxiv - Cancer Biology 2020Quote: ... TRIP12 CDS was subcloned into the LHCX vector (Clontech, Cat. No. 631511) using a single NotI site introduced into the LHCX vector through the HindIII site.
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bioRxiv - Developmental Biology 2021Quote: ... NKX2.1 CDS harbouring each mutation was amplified and inserted by Infusion (638909, Takara) cloning into the tetON-NKX2.1/EF1a-TagRFP-2A-tet3G Dox-inducible lentiviral vector ...
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bioRxiv - Cancer Biology 2023Quote: ... the target mRNA CDS sequence was cloned using PrimeSTAR® Max (TAKARA, R045) or Q5® High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... the CDS of TaNAC016-3A were fused into the yeast vector pGADT7 (Clontech) to generate AD-NAC016-3A prey plasmid ...
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bioRxiv - Bioengineering 2021Quote: ... 1/3 volume Retro-X concentrator (Takara) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3 μM Shield-1 (Takara Bio) for indicated times ...
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bioRxiv - Cancer Biology 2022Quote: ... MB CDS were ligated in XhoI and BamHI digested pmCherry-C1 (Takara Bio #632524) or in HindIII and BamHI digested pmCherry-N1 (Takara Bio #632523 ...
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bioRxiv - Developmental Biology 2024Quote: ... The CDs of cdon was amplified using PCR (Prim STAR Max Premix Takara NO.R045A) and cloned into the vector PCS2+ to generate the expression constructs (5x In-Fusion HD Enzyme Premix ...
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bioRxiv - Genetics 2019Quote: BAC clone (BAC PAC RPCI- 98 library) DNA was extracted using a MIDI-prep kit (Clontech #740410). The probe for the bw locus was Clone BACR48M01 ...
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bioRxiv - Plant Biology 2020Quote: All SUMO CDS were translationally fused with the activation domain of pGADT7 AD (Takara Bio). βC1 was fused with binding domain and cloned into pGBKT7 BD (Takara Bio) ...
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bioRxiv - Plant Biology 2022Quote: ... we fused proAtSPCH and MpSETA CDS fragments by PCR using PrimeSTAR GXL polymerase (Takara Bio). The resultant PCR fragment was subcloned into pENTR1A entry clones (Invitrogen ...
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bioRxiv - Synthetic Biology 2020Quote: ... including the promoter and the full CDS was amplified by high-fidelity PCR (TaKaRa Bio) and sequenced on an Illumina MiSeq system with transposase based library preparation kit (Nextera XT ...
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bioRxiv - Plant Biology 2020Quote: ... the CDS of ONAC127 and ONAC129 was cloned into the pGBKT7 and pGADT7 vector (Clontech). The fusion plasmids were then transformed into yeast strain AH109 or Y187 ...
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bioRxiv - Evolutionary Biology 2022Quote: CDS sequence coding protein BmMETTL3(204-329) was cloned into the Pcold vector (TaKaRa, China). Primers were listed in Table S1 ...
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bioRxiv - Plant Biology 2022Quote: The coding sequence (CDS) of SAUR30 was cloned using Prime STAR MAX polymerase mix (TaKaRa) and the gene-specific primers shown in Supplemental Table S1 ...
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bioRxiv - Plant Biology 2020Quote: ... Full-length CDS of FveIAA4 and FveGID1c were ligated into GAL4 BD vector PBGK T7 (Clontech Inc.) through EcoRI/SalI double digestion sites ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 1/3 volume Lenti-X Concentrator (Takara 631232), mixed by gentle inversion ...
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bioRxiv - Cancer Biology 2023Quote: ... lentiviral constructs pCMV-VSV-G and pCMV-dR8.2 dvpr 98 were transfected in HEK293T cells by the calcium-phosphate method (CalPhos Mammalian Transfection Kit, Takara, 631312) for virus production ...
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bioRxiv - Developmental Biology 2019Quote: ... The CDS of the EGFP and mCherry fluorescent tags were obtained by PCR from the pEGFP-C1 (Clontech) and pRSET-B-mCherry (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... The full-length CDS features of those genes were amplified by PrimeSTAR Max DNA Polymerase (Takara Bio, Japan) with each primer set and cloned into the BamHI and Hind III sites of the pCold I vector by In-Fusion Cloning (Takara Bio ...
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bioRxiv - Genomics 2019Quote: ... the beads were washed twice with 50 μl 80% freshly-prepared EtOH on the magnetic stand and the dried beads pellets were resuspended in 4 μL elution buffer (RNAse inhibitor 1/200 Takara, CDS primer 0.5 μL in RNAse free water) immediately followed by an incubation at 72°C for 3 min ...
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bioRxiv - Immunology 2022Quote: ... or the expression vector LentiCRISPRv2 (coding for guideRNA) in a 3:1:3 ratio for 8 hours using calcium phosphate transfection (CalPhos Mammalian Transfection Kit, Takara Bio Europe ...
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bioRxiv - Molecular Biology 2021Quote: ... were used to amplify the full-length coding sequences CDS amplified with Tks Gflex™ DNA Polymerase (TaKaRa, Japan) according to manufacturer’s directions with an annealing temperature of 56.4°C ...
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bioRxiv - Plant Biology 2019Quote: ... The RxL10 CDS was recombined from pDONR207 into a Gateway compatible version of the Y2H vector pGilda (Clontech, Mountainview, CA) to generate an N-terminal fusion to the LexA DNA binding domain ...
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bioRxiv - Cell Biology 2019Quote: Expression plasmid for mouse FLAG tagged BAG3 (pFLAG-BAG3) was constructed by cloning partial mouse BAG3 cDNA containing the whole CDS into pEGFP-N1 (Clontech). Forward primer sequence used to clone BAG3 plasmid is 5′-AAAGGATCCAGCGCCGCCACCC-3′ and the reverse primer sequence is 5′-GACTCTAGATCACTAGGGAGCCACCAGGTTGC-3′ ...
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bioRxiv - Plant Biology 2020Quote: ... The BrSRK-9 CDS fragment was introduced into the KpnI site of pMYC290 by InFusion cloning (TAKARA Bio, Shiga, Japan). Fragments of CDS of the BrSRK S domain and transmembrane domain were amplified by PCR using the first-strand cDNA synthesized from stigma RNA (template ...
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bioRxiv - Cell Biology 2021Quote: ... BicD and GFP CDS were amplified by PCR and the two fragments were combined into AttB-pUASp-K10 vector by InFusion cloning (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... All PIF3 constructs for the yeast transactivation assays were generated by subcloning either the full-length or a fragment of PIF3 CDS into the EcoRI/SalI sites of the pBridge vector (Clontech). The GST-PIF3 and GST-PIF3mTAD constructs for GST pulldown assays were generated by subcloning PIF3 or PIF3mTAD into the EcoRI/XhoI sites of the pET42b vector (Promega) ...
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bioRxiv - Plant Biology 2021Quote: ... the CDS of SWEET5 was amplified by primers P17 and P18 and subcloned into it by In-Fusion® (Takara). The agroinfiltration-based assays were performed as previously described (Gookin and Assmann ...
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bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing CDS of each Hero protein and C-terminal FLAG and His tags was inserted into pCold I (Takara) by NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Plant Biology 2023Quote: ... and was cloned into the AscI site of the pENTR/D-TOPO entry vector containing full length MpNEK1 CDS (Otani et al., 2018) by In-fusion system (Takara). The resulting vector pENTR/D-TOPO-MpNEK1-mCitrine was subjected to LR reaction to transfer MpNEK1-Citrine fusion into the Gateway binary vector pMpGWB144 (MpEF1α pro:XVE >>LexA operator:Gateway cassette).
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bioRxiv - Immunology 2023Quote: ... into 96-well plates containing 12.5μl CDS sorting buffer as described in the SMART-Seq® HT Kit protocol (Takara Bio). Full-length cDNA amplification of single cells was performed per the kit protocol (Takara Bio) ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... was digested with NheI and KpnI and the Lmx1a CDS and IRES-GFP fragments were inserted using the In-Fusion HD Cloning kit (Takara). Molecular biology kits used are listed in Table T4.
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bioRxiv - Genomics 2024Quote: ... Supernatant containing viral particles was harvested after 2 and 3 days and concentrated 100x using Lenti-X concentrator (Takara) following manufacturers instructions.
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... to OD600 0.2–0.3 with 2–3 mL fresh AYE +Fe +Cys containing 40 ng/mL anhydrous tetracycline (aTC, Clontech 631310). On the third day ...
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bioRxiv - Neuroscience 2021Quote: ... three types of the GPR151 CDS-related inserts were sub-cloned into FUGW vector using In-Fusion Cloning kit (Takara, 638910). For control experimental sets ...
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bioRxiv - Neuroscience 2022Quote: ... was digested using HinDIII and XhoI to remove the PSMD2 CDS and the amplified Zic3 gene was cloned using Infusion kit (Clontech, USA) as per manufacturer’s instruction. ...
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bioRxiv - Plant Biology 2022Quote: ... StNPR1 or StNPR3/4 cds) construct combinations were transformed into the Y2H Gold strain using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, USA) and the transformants selected on control SD media without Leu and Trp (-L-W) ...