Labshake search
Citations for Takara Bio :
1 - 50 of 389 citations for Inactive Serine Protease 35 PRSS35 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... Ras family GTPases lacking C-terminal cysteine residues and with mutations locking them in either the active or inactive conformation were cloned into pcDNA3.1+ (Clontech), upstream of a GAGA linker ...
-
bioRxiv - Cell Biology 2021Quote: Mouse IGFBP3 cDNA fused with a C-terminal 3xFlag (linked with a glycine-glycine-glycine-glycine-serine segment) was cloned into pQXCIP (Clontech) retroviral vector and sequence-confirmed ...
-
bioRxiv - Immunology 2021Quote: ... PCR was performed for 35 cycles using PrimeStar MAX (Takara). Primers used for these reactions can be found in Supplementary Table 1 ...
-
bioRxiv - Neuroscience 2023Quote: ... HRV3C protease (TAKARA) was added overnight at 4°C and Ni-NTA was added to eliminate the cleaved portion overnight at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... protease inhibitor (Takara, Japan), primary antibody (Cell signaling technology ...
-
bioRxiv - Molecular Biology 2021Quote: ... and then used as a template for PCR for 35 cycles with EpiTaq (TAKARA) using the following primer ...
-
bioRxiv - Immunology 2021Quote: ... PCR on the resulting cDNA was performed for 35 cycles using PrimeStar MAX (Takara). Then ...
-
bioRxiv - Plant Biology 2023Quote: ... under the control of cauliflower mosaic virus 35S promoter in the binary vector pBI121 (Clontech, USA) was transferred into Agrobacterium tumefaciens strain GV3101 and used to transform Arabidopsis by floral dip method and tobacco plants by leaf disk method (Horsch et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... and protease inhibitors (Takara Bio, Kusatsu, Japan) mixes ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1X protease inhibitor cocktail (635673; Takara Clontech), 1 mM PMSF ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1X protease inhibitor cocktail (635673; Takara Clontech), 1 mM PMSF ...
-
bioRxiv - Genomics 2019Quote: ... 1 ng full length cDNA was used to perform 35-cycle PCR with Premix Taq™ (TaKaRa). PCR products were purified with QIAquick Gel Extraction Kit (Qiagen ...
-
bioRxiv - Microbiology 2022Quote: Vero cells were transduced with supernatants of Plat-GP cells cotransfected with pMDG (35) and pRetroX-Tet3G (TaKaRa), selected with 1 mg/mL G418 solution (Wako ...
-
bioRxiv - Neuroscience 2020Quote: ... containing 1% protease inhibitor (Clontech, Mountain View, CA) and 1% phosphatase inhibitors (Millipore Sigma) ...
-
bioRxiv - Neuroscience 2019Quote: ... containing 1% protease inhibitor (Clontech, Mountain View, CA) and 1% phosphatase inhibitors (Millipore Sigma) ...
-
bioRxiv - Plant Biology 2023Quote: ... 0.05% T-20 and protease inhibitor cocktail (Takara)] ...
-
bioRxiv - Genomics 2021Quote: ... Sample suspension (35 nL) was dispensed on the ICELL8 into the nanowells of a 350v Chip (Takara Cat# 640019). The 350v Chip was dried and sealed ...
-
bioRxiv - Microbiology 2021Quote: ... 10 U ml−1 of HRV- 3C protease (Takara) were added to the Ni-NTA eluted protein to remove the oligohistidine- GST-tag during dialysis against 3 L of dialysis buffer I (25 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2022Quote: ... GST tags were cleaved by HRV-3C Protease (Takara), which released the recombinant proteins from the beads ...
-
bioRxiv - Plant Biology 2020Quote: ... The CDS of PuACO1 was cloned into the KpnI and EcoRI sites downstream of the GFP sequence and the CaMV 35S promoter in the pRI101 vector (TaKaRa). The recombinant Pro35S:Myc-PuBZR1 and Pro35S:GFP-PuACO1 constructs were infiltrated into tobacco (N ...
-
bioRxiv - Cancer Biology 2021Quote: ... 24 cycles (for actin beta [ACTB]) or 35 cycles (for MLKL) of amplification were performed under the following conditions using PrimeSTAR Max DNA Polymerase (Takara): denaturing at 98°C for 10 s ...
-
bioRxiv - Microbiology 2021Quote: ... pBICP35-CE1–CE8 transient expression vectors under the control of the 35S promoter were constructed using an In-Fusion system (Clontech, TaKaRa). The fragment containing the cDNA of CE1 was amplified with the primers 35S_CE1_Fw and 35S_CE1_Rv ...
-
bioRxiv - Plant Biology 2021Quote: The Pro197Ser-mutated ALS genes and the native form of MvALS1 gene were inserted into the pCAMBIA1390 vector under the cauliflower mosaic virus 35S promoter using the In-Fusion DH Cloning Kit (TaKaRa), as described previously (Iwakami et al. ...
-
bioRxiv - Microbiology 2021Quote: ... 72 °C 20 seconds for 35 cycles followed by subcloning into the eukaryotic expression vector pCAGGs using the In-Fusion HD cloning kit (Takara). All plasmids were midiprepped using the Nucleobond Xtra midi kit (Macherey-Nagel) ...
-
bioRxiv - Genetics 2019Quote: ... The gene fragments of off-target sites were amplified with primers specific to each locus by 35 cycles of PCR with TaKaRa LA Taq (TaKaRa). The PCR products were purified and subjected to the process of denatured and annealed by using a thermocycler ...
-
bioRxiv - Plant Biology 2019Quote: The full-length cDNA of NaERF2-like with two HA flags was cloned into pCAMBIA1301 vector after 35S promoter via in-fusion technique (Clontech). N ...
-
bioRxiv - Plant Biology 2019Quote: ... EPFL2 promoter DNA was amplified by PCR from Col genomic DNA and inserted at the HindIII and SmaI sites of pPZP211/35S using the InFusion kit (Clontech) yielding the intermediate vector pPZP211/pEPFL2 ...
-
bioRxiv - Plant Biology 2022Quote: ... which was then cloned into the Aor51HI site in pUGW2 35S 97 using the In-Fusion cloning kit (TaKaRa Bio). The 2.5-kb HindIII-SacI fragment in the resulting plasmid ...
-
bioRxiv - Microbiology 2023Quote: PCRs were conducted over 35 cycles and were based on TaKaRa Ex Taq Hot Start polymerase chemistry (Takara Bio, USA). For 16S rRNA gene amplification ...
-
bioRxiv - Plant Biology 2023Quote: ... The amplified cDNA was inserted downstream of the CaMV 35S promoter in the modified pRI201-AN vector using In-Fusion Cloning technology (Takara). Agrobacterium tumefaciens strain C58 ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was collected after centrifugation at 65,000 g for 35 min and was incubated with TALON® Metal Affinity Resin (TaKaRa) for 2 h at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... The PCR product was run on a 1% agarose gel at 85 V for 35 min and gel extracted using the Nucleospin Gel Extraction Kit (Takara). We then used PCR with KOD Polymerase 2x Mastermix (Millipore Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... Cleavage of the His-tag by HRV3C protease (Takara, 7360) followed the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... and 10% glycerol with complete protease inhibitor cocktail (Clontech Laboratories). Cells were incubated on ice for 20 minutes before centrifugation at 14,000 rpm at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... pBICP35-CE1–CE8 transient expression vectors under the control of the 35S promoter were constructed using an In-Fusion system (Clontech, TaKaRa). The fragment containing the cDNA of CE1 was amplified with the primers 35S_CE1_Fw and 35S_CE1_Rv ...
-
bioRxiv - Plant Biology 2021Quote: ... Products were ligated to pCAMBIA1300 vector backbone containing the 35S promoter and a C-terminal YFP using the In-Fusion cloning system (Takara Bio).
-
bioRxiv - Plant Biology 2020Quote: ... plant intron Syn7 [34] regulated by a double-enhancer Cauliflower mosaic virus 35S RNA promoter (P35S2) and a nos terminator in the T-DNA binary vector pBI121 (Clontech, Switzerland). Syn7 was amplified with the primers 5′- GAGCTGAAAGggtaagattatcgatatttaaattatttatttcttcttttc-3′ and 5′-TGAAGTCGATGCctgcatgatatcataaaaccatgga-3′ specifying the splicing junctions and then inserted into the YFP coding region by overlapping PCR ...
-
bioRxiv - Genetics 2020Quote: ... The PE Sox1(+35)TFBS or Sox1(+35)TFBS+CGI inserts in Gata6-TAD or Gria1-TAD were amplified by PCR using EpiTaq polymerase (Takara Bio) and primers described in Extended Data Fig ...
-
bioRxiv - Cancer Biology 2023Quote: ... Michael Kilberg, [35]) into a plasmid expressing both luciferase and tdTomato (pFULT, kind gift of Prof. Ghambir) using InFusion (Takara Bio) according to the manufacturer’s specifications.
-
bioRxiv - Neuroscience 2021Quote: ... vector used was AAV9-CaMKII::G-CaMP7 (9.4 × 1012 vg/mL)35 after 10-fold dilution with phosphate buffered saline (PBS) (T900, Takara BIO INC, Japan) before injection ...
-
bioRxiv - Microbiology 2022Quote: ... S-6P-NanoLuc proteins were released after HRV 3C protease (TaKaRa) treatment overnight at 4°C.
-
bioRxiv - Molecular Biology 2022Quote: ... Lysozyme solution and Protease Inhibitor Cocktail (Takara Bio USA, Inc., CA). The suspension was sonicated 10 sec for 3 times with a 30 s pause ...
-
bioRxiv - Microbiology 2020Quote: ... Purification tags were removed by treating recombinant proteins with HRV3C protease (TaKaRa) and cOmplete™ His-tag Purification Resin (Roche) ...
-
bioRxiv - Cell Biology 2021Quote: ... and pH7.2) supplemented with the ProteoGuard EDTA-free protease inhibitor cocktail (Clontech, 635673). The cells in the suspended solution were sonicated using the Q125 sonicator (Qsonica ...
-
bioRxiv - Cell Biology 2022Quote: ... The remaining HRV-3C Protease was removed by TALON Metal Affinity Resin (Takara). In the rescue assays ...
-
bioRxiv - Cancer Biology 2020Quote: ... The primary antibody rabbit anti-human Cas9 Polyclonal Antibody (Clontech) was diluted 1 ...
-
bioRxiv - Microbiology 2022Quote: ... monoclonal antibody (Clontech) and peroxidase-labeled anti-mouse IgG (H + L ...
-
bioRxiv - Biochemistry 2023Quote: ... The His-tagged TEV protease was removed by binding to Co2+-charged TALON resin (Clontech) and the flow-through concentrated using a 100 kDa molecular weight cut off concentrator (Corning) ...
-
bioRxiv - Microbiology 2023Quote: ... anti-GAPDH antibody (Clontech) at a 1/2500 dilution ...
-
bioRxiv - Genetics 2019Quote: ... Antibodies used were GAL4 AD Mouse Monoclonal Antibody (Takara Bio USA, Inc. #630402), GAL4 DNA-BD Mouse Monoclonal Antibody (Takara Bio USA ...