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1 - 50 of 263 citations for IGF I Rat since 2019

• Forced isoform switching of Neat1_1 to Neat1_2 leads to the loss of Neat1_1 and the hyperformation of paraspeckles but does not affect the development and growth of mice

  Momo Isobe, et al., bioRxiv - Molecular Biology 2019

  Quote: ... After DNase I treatment (Recombinant DNase I, TaKaRa #2270A), cDNAs were synthesized with ReverTra Ace qPCR RT Master Mix (TOYOBO ...
• Widespread multi-targeted therapy resistance via drug-induced secretome fucosylation

  Mark Borris D. Aldonza, et al., bioRxiv - Cancer Biology 2021

  Quote: ... RNA was treated with deoxyribonuclease I (DNase I; Takara) and reverse-transcribed using RevertAid reverse transcriptase (Fermentas) ...
• Identification and Characterization of Stem Cells in Mammalian Esophageal Stratified Squamous Epithelia

  Yanan Yang, et al., bioRxiv - Cell Biology 2021

  Quote: ... DNase I (TaKaRa) was added to each sample ...
• Mitochondrial dsRNAs activate PKR and TLR3 to promote chondrocyte degeneration in osteoarthritis

  Sujin Kim, et al., bioRxiv - Molecular Biology 2020

  Quote: ... DNase I (TaKaRa) was treated to remove DNA ...
• Bi-phasic regulation of AIMP2 and its splice variant in PARP-1-dependent neurodegeneration

  Min Hak Lee, et al., bioRxiv - Molecular Biology 2022

  Quote: ... and deoxyribonuclease I (DNase I, 5 units/mL, Takara, Shiga, Japan) for 15 min ...
• Cell-free multi-omics analysis reveals tumor status-informative signatures in gastrointestinal cancer patients’ plasma

  Yuhuan Tao, et al., bioRxiv - Systems Biology 2023

  Quote: ... Recombinant DNase I (TaKaRa) was used to digest DNAs ...
• Discovery of prevalent natural Wolbachia in Aedes aegypti in Metropolitan Manila, Philippines using locally designed primers: bacterial density is influenced by strain and host sex

  Jerica Isabel L. Reyes, et al., bioRxiv - Microbiology 2022

  Quote: ... and exonuclease I (TaKaRa). The cleaned samples were subjected to Sanger sequencing for strain identification.
• FOP plays a negative role in ciliogenesis and promotes cell cycle re-entry by facilitating primary cilia disassembly

  Huadong Jiang, et al., bioRxiv - Cell Biology 2019

  Quote: ... cut with EcoR I and Xho I and then inserted into pEGFP-N1 (Clontech). The construct was verified by DNA sequencing.
• The Copper Responsive Transcription Factor SPL7 Represses Key Abscisic Acid Biosynthetic Genes to Balance Growth and Drought Tolerance

  Yanzhi Yang, et al., bioRxiv - Plant Biology 2021

  Quote: ... treated with DNase I (TaKaRa), and reverse transcribed using the PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) ...
• Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis

  Yi Sun, et al., bioRxiv - Plant Biology 2022

  Quote: ... followed by DNase I (Takara) treatment ...
• Uncovering novel endolysins against methicillin-resistant Staphylococcus aureus using microbial single-cell genome sequencing

  Takuya Yoda, et al., bioRxiv - Microbiology 2023

  Quote: ... and DNase I (Takara Bio) on ice until the bacterial cell are lysed ...
• Novel in-silico predicted matrikines are differential mediators of in vitro and in vivo cellular metabolism

  Nathan Jariwala, et al., bioRxiv - Cell Biology 2023

  Quote: ... procollagen type I (Takara #MK101), fibronectin (Takara #MK115) ...
• Generation of rat lungs by blastocyst complementation in Fgfr2b-deficient mouse model

  Shunsuke Yuri, Yuki Murase, Ayako Isotani, bioRxiv - Developmental Biology 2022

  Quote: ... rat anti-E-Cadherin (TAKARA; M110), rat anti-Endomucin (Santa Cruz ...
• Single-cell RNA-sequencing analysis reveals the molecular mechanism of subchondral bone cell heterogeneity in the development of osteoarthritis

  Yan Hu, et al., bioRxiv - Pathology 2022

  Quote: ... Rat anti Mouse OCN (Takara, M188); Rabbit anti Mouse MGST1 (Abcam ...
• MRSA emerges through natural transformation

  Mais Maree, et al., bioRxiv - Microbiology 2020

  Quote: ... The PCR product was cloned into the EcoR I – BamH I site of pHY300PLK (Takara) to generate the complementation plasmids pHY-12 ...
• The trans-zeatin-type side-chain modification of cytokinins controls rice growth

  Takatoshi Kiba, et al., bioRxiv - Plant Biology 2022

  Quote: ... The amplified fragments were cloned into the Xba I/Sac I site of pBI121 (Clontech) located downstream of the cauliflower mosaic virus (CaMV ...
• DR5 disulfide bonding as a sensor and effector of protein folding stress

  Mary E. Law, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and Cla I sites 5′ to the BamH I site (Clontech, Mountain View, CA, USA).
• A novel culture-independent method to search unknown dominant bacterial groups and its application to microbial analysis of membrane bioreactors

  Katsuji Watanabe, Naoto Horinishi, bioRxiv - Microbiology 2020

  Quote: ... using each of the following 4 restriction enzymes: HaeIII or Hha I or Rsa I or Alu I (10 units, Takara Bio Co. Ltd. Shiga, Japan) in buffer solution (10xLow salt buffer ...
• Tracking pre-mRNA maturation across subcellular compartments identifies developmental gene regulation through intron retention and nuclear anchoring

  Kyu-Hyeon Yeom, et al., bioRxiv - Molecular Biology 2020

  Quote: RNA was treated DNase I (Takara) followed by phenol extraction ...
• Crosstalk between Fat Mass and Obesity-related (FTO) and multiple WNT signaling pathways

  Hyunjoon Kim, Soohyun Jang, Young-suk Lee, bioRxiv - Molecular Biology 2021

  Quote: ... and treated with DNase I (Takara). 200-500 ng of total RNAs were reverse transcribed using Primescript RT master mix (Takara) ...
• Extracellular matrix mineralization in the mouse osteoblast-like cell line MC3T3-E1 is regulated by actin cytoskeleton reorganization and non-protein molecules secreted from the cells themselves

  Hiraku Suzuki, et al., bioRxiv - Biochemistry 2019

  Quote: ... and Recombinant RNAse i nhibitor (TaKaRa). cDNA was prepared from total RNAs using ReverTra Ace (TOYOBO) ...
• Versatile and multiplexed mass spectrometry-based absolute quantification with cell-free-synthesized internal standard peptides

  Keiko Masuda, et al., bioRxiv - Synthetic Biology 2021

  Quote: ... restriction enzyme Dpn I (Takara Bio) was added to cut and remove the original template plasmid at 37 °C overnight ...
• Dorsomedial hypothalamic BDNF neurons integrate thermal afferent signals to control energy expenditure

  Qian Zhou, et al., bioRxiv - Neuroscience 2021

  Quote: ... after DNase I treatment (Takara, #RR047A). PCR was performed in triplicate using an SYBR Green PCR kit (Takara ...
• Overexpression of the RNA-binding protein NrdA affects global gene expression and secondary metabolism in Aspergillus species

  Chihiro Kadooka, et al., bioRxiv - Microbiology 2021

  Quote: The plasmid pPTR I (Takara Bio), which carries the ptrA gene ...
• Overexpression of the RNA-binding protein NrdA affects global gene expression and secondary metabolism in Aspergillus species

  Chihiro Kadooka, et al., bioRxiv - Microbiology 2021

  Quote: The plasmid pPTR I (Takara Bio) was used to construct the overexpression vector for the A ...
• Overexpression of the RNA-binding protein NrdA affects global gene expression and secondary metabolism in Aspergillus species

  Chihiro Kadooka, et al., bioRxiv - Microbiology 2021

  Quote: ... or plasmid pPTR I (Takara Bio) were used as template DNA ...
• Overexpression of the RNA-binding protein NrdA affects global gene expression and secondary metabolism in Aspergillus species

  Chihiro Kadooka, et al., bioRxiv - Microbiology 2021

  Quote: ... pPTR I (Takara bio, Shiga, Japan) (47 ...
• Overexpression of the RNA-binding protein NrdA affects global gene expression and secondary metabolism in Aspergillus species

  Chihiro Kadooka, et al., bioRxiv - Microbiology 2021

  Quote: ... and plasmid pPTR I (Takara Bio) were used as template DNA ...
• Chromatin-based mechanisms to coordinate convergent overlapping transcription

  Soichi Inagaki, et al., bioRxiv - Molecular Biology 2020

  Quote: ... and treated with DNase I (Takara). Libraries for mRNA-seq were constructed using the KAPA Stranded mRNA-seq Kit according to the manufacturer’s instruction ...
• Evolutionary transition from a single RNA replicator to a multiple replicator network

  Ryo Mizuuchi, et al., bioRxiv - Evolutionary Biology 2021

  Quote: ... after digestion with Sma I (Takara). The remaining plasmids were treated with DNase I (Takara) ...
• Identification and functional prediction of sugar beet circRNAs involved in drought responses

  Chun-Lei Zou, et al., bioRxiv - Plant Biology 2022

  Quote: ... DNase I (Takara Bio, Dalian, China) was used to process the total RNA ...
• Cotranscriptional demethylation induces global loss of H3K4me2 from active genes in Arabidopsis

  Shusei Mori, et al., bioRxiv - Plant Biology 2023

  Quote: ... and treated with DNase I (Takara). Libraries for mRNA-seq were constructed using the KAPA Stranded RNA-seq Library Preparation Kit according to the manufacturer’s instructions ...
• Cotranscriptional demethylation induces global loss of H3K4me2 from active genes in Arabidopsis

  Shusei Mori, et al., bioRxiv - Plant Biology 2023

  Quote: ... and treated with DNase I (Takara). Libraries for mRNA-seq were constructed using the KAPA Stranded RNA-seq Library Preparation Kit according to the manufacturer’s instructions ...
• Uncovering novel endolysins against methicillin-resistant Staphylococcus aureus using microbial single-cell genome sequencing

  Takuya Yoda, et al., bioRxiv - Microbiology 2023

  Quote: ... added 0.2U of DNase I (Takara), and incubated at 30 °C for an hour ...
• Piezo1 Mutant Zebrafish as a Model of Idiopathic Scoliosis

  Ramli, et al., bioRxiv - Developmental Biology 2023

  Quote: ... treated with DNase I (Takara Bio), and purified by RNA clean and concentration kit (Zymo Research).
• Mixed Culture of Bacterial Cell for Large Scale DNA Storage

  Min Hao, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Then QuickCut™ Not I (Takara, #1623) was used for fragments recovery ...
• CoBATCH for high-throughput single-cell epigenomic profiling

  Qianhao Wang, et al., bioRxiv - Genomics 2019

  Quote: ... 10 U/ml DNase I (TaKaRa 2270A) was added to the reaction after 3-min digestion and gently pipetting was repeated every 5-6 min digestion ...
• Escherichia coli small heat shock protein IbpA is an aggregation-sensor that self-regulates its own expression at a translational level

  Tsukumi Miwa, Yuhei Chadani, Hideki Taguchi, bioRxiv - Biochemistry 2020

  Quote: ... and treated with recombinant DNase I (Takara). The treated RNA was purified with an RNeasy Mini kit (Qiagen) ...
• Genome-wide Transcriptomic Analysis of the Response of Botrytis cinerea to Wuyiencin

  Liming Shi, et al., bioRxiv - Microbiology 2019

  Quote: ... RNase-free DNase I (Takara Bio, Japan) was used to digest traces of genomic DNA ...
• Functional unit and regulatory mechanisms of supergene in female-limited Batesian mimicry of Papilio polytes

  Shinya Komata, et al., bioRxiv - Genetics 2022

  Quote: ... The extracted and DNase I (TaKaRa, Japan) treated RNA was sent to Macrogen Japan Corporation for library preparation by TruSeq stranded mRNA (paired-end ...
• Virome and nrEVEome diversity of Aedes albopictus mosquitoes from La Reunion Island and China

  Umberto Palatini, et al., bioRxiv - Genomics 2022

  Quote: ... treated with DNase I (Takara, Kusatsu, Japan) and stored at -80 °C ...
• Rec8 cohesin-mediated axis-loop chromatin architecture is required for meiotic recombination

  Takeshi Sakuno, et al., bioRxiv - Cell Biology 2022

  Quote: ... Fifty units of DNase I (TaKaRa Bio) were added to the cell extract ...
• Programmable acetylation modification of bacterial proteins by a Cas12a-guided acetyltransferase

  Yanqiang Liu, Ni Zuo, Weihong Jiang, Yang Gu, bioRxiv - Microbiology 2022

  Quote: ... and further treated with DNase I (TaKaRa) to eliminate residual DNA contained in the extracted samples ...
• Molecular mechanisms underlying the formation of larval green color and camouflage patterns in swallowtail butterfly, Papilio memnon

  Liang Liu, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and treated by DNase I (TaKaRa, Japan). The total RNAs were sent to Macrogen Japan Corp ...
• Comparable analysis of multiple DNA double-strand break repair pathways in CRISPR-mediated endogenous tagging

  Chiharu Tei, et al., bioRxiv - Genomics 2023

  Quote: ... After treatment of DNase I (Takara Bio), the synthesized guide RNA was purified using the RNA Clean & Concentrator Kit (Zymo Research) ...
• The Escherichia coli small heat shock protein IbpA plays a role in regulating the heat shock response by controlling the translation of σ³²

  Tsukumi Miwa, Hideki Taguchi, bioRxiv - Molecular Biology 2023

  Quote: ... and treated with recombinant DNase I (Takara). The treated RNA was subsequently purified using an RNeasy Mini kit (Qiagen) ...
• Depletion-assisted multiplexed cell-free RNA sequencing reveals distinct human and microbial signatures in plasma versus extracellular vesicles

  Hongke Wang, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and treated with DNase I (TaKaRa, 2270A) for 20 min at 37 °C ...
• Discovery of N-Glycan Outer Chain like α-(1→6)-linked Mannan Structures in Aspergillus fumigatus Mycelium

  Chihiro Kadooka, et al., bioRxiv - Microbiology 2023

  Quote: ... utilizing the plasmid pPTR I (TAKARA, Japan) as a template and the primer pairs pHSG396-ptrA-IF-F and pHSG396-ptrA-IF-R ...
• The Shank/ProSAP N-terminal (SPN) domain of Shank3 regulates targeting to postsynaptic sites and postsynaptic signalling

  Daniel Woike, et al., bioRxiv - Neuroscience 2023

  Quote: ... Rat Shank3 deletion constructs in pmRFP-N3 (Clontech) were described before (23) ...
• Murine uterine gland branching is necessary for gland function in implantation

  Katrina Granger, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rat anti-CDH1 (M108, Takara Biosciences, 1:500), rabbit anti-FOXA2 (Abcam ...