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Citations for Takara Bio :
1 - 50 of 878 citations for IFN γ Receptor II Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... CHO-K1 cells were transfected with ACP-human IR plasmid using Xfect transfection reagent (Takara). 1 day after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Human Universal QUICK-Clone cDNA II (Clontech) for a template cDNA ...
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bioRxiv - Immunology 2021Quote: ... Full-length mIDO1 gene was PCR amplified from cDNA of IFN-γ-treated ID8 cell line (26) and inserted into the first cloning site in a retrovirus backbone vector (pQCXIX, Clontech-TaKaRa). A fusion gene of codon-optimized Luciferase (Luc2 ...
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bioRxiv - Neuroscience 2022Quote: ... were cotransfected into CHO-tetON cells (Clontech). Stably expressing cell lines were selected for by the addition of 1mg/mL hygromycin B to the media and single colonies were picked to generate monoclonal stable cell lines ...
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bioRxiv - Immunology 2020Quote: ... Human MTC™ Panel II (Takara Bio, Mountain View, CA, USA) were used ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 10 ng cDNA of various tissues (Takara Human MTC panel I & II) were used for each reaction and amplified by KOD Xtreme Hot Start DNA polymerase kit (Takara) ...
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bioRxiv - Immunology 2021Quote: ... T cell receptor sequencing libraries were prepared with the SMARTer Human TCR α/β Profiling Kit (catalog number 635015, Takara Bio USA, Inc.) according to manufacturer’s instructions with the exception of excluding the third and fourth bead size selection steps listed in Table 3 of the kit manual ...
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bioRxiv - Immunology 2021Quote: ... with the modification that Ecotropic Receptor Booster (Takara, #631471) was added to the cells as suggested by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... CHO-K1 cells were co-treated using 1μg/mL doxycycline (Takara Bio Europe, Clontech Inc.) along with the small molecules for experiments as described in main text.
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bioRxiv - Bioengineering 2019Quote: ... HEK293T and CHO-K1 cell lines were ordered from American Type Culture Collection (ATCC) and HEK293-tetON cells and CHO-tetON cells were purchased form Clontech (Takara Bio). Synthetic DNA was ordered from Twist Bioscience.
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bioRxiv - Cell Biology 2021Quote: ... CHO-K1 cells were co-treated using 1μg/mL doxycycline (Takara Bio Europe, Clontech Inc.) along with the small molecules for experiments as described in main text.
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bioRxiv - Cell Biology 2020Quote: ... at the C-terminus were amplified by PCR using KOD-Plus-Neo polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) for a template cDNA and then cloned into a pET-41 Ek/LIC vector (Novagen) ...
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bioRxiv - Cell Biology 2020Quote: ... 3C protease-cleavage site and a His12-tag at the N-terminus were amplified by PCR using KOD-Plus-Neo polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) as a template cDNA and then cloned into a pET-41 Ek/LIC vector (Novagen) ...
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bioRxiv - Neuroscience 2022Quote: The Yeast-Two-Hybrid screening for Jacob interaction partners was performed using a pretransformed human brain cDNA Library in pACT2 (Matchmaker-GAL4 Two-Hybrid II; Clontech) as described previously (Helmuth et al. ...
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bioRxiv - Cell Biology 2019Quote: ... The coding sequences of these small GTPase proteins were amplified with polymerase chain reaction (PCR) using KOD-Plus-Neo DNA polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) as template cDNA ...
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bioRxiv - Neuroscience 2021Quote: The desired ORF for Pdlim7 (Gene bank ID: AF345904.1) was amplified by PCR from Human Universal QUICK-Clone™ II (Takara 637260) using primers depicted in Table S2 and inserted into pEGFP-C1 plasmid through Gibson assembly.
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bioRxiv - Neuroscience 2023Quote: ... was similarly constructed with the leptin receptor promoter controlling expression of mCherry (Clontech) without ChR2 ...
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bioRxiv - Cell Biology 2021Quote: ... PrimeScript II (Takara) was used for the reverse transcription ...
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bioRxiv - Genomics 2019Quote: ... and the expression of IFN-β and IL-6 were measured by quantitative PCR (TAKARA, RR820A). The sequences of the primers were listed in the Supplementary Table 4.
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bioRxiv - Bioengineering 2024Quote: ... and the receptor- or payload-encoding viral expression plasmids (2.0 μg) into Lx293T cells (Clontech) in 6-well plates with Lipofectamine 3000 (Thermo Fisher L3000001) ...
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bioRxiv - Molecular Biology 2020Quote: ... They were radiolabeled at their 5′ ends with [γ-32P] ATP by T4 polynucleotide (Takara) and purified using Oligo Clean & Concentrator (Zymo Research ...
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bioRxiv - Molecular Biology 2020Quote: Primers (PAGE grade, fasmac, Appendix Table S4) were labelled with 32P-γ-ATP by T4PNK (Takara), followed by filtering through MicroSpin G-25 Columns (GE healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... Antisense probes were radiolabeled at their 5′ ends with [γ-32P] ATP by T4 polynucleotide (Takara) and purified using Performa Spin Columns (Edge BioSystems) ...
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bioRxiv - Immunology 2024Quote: ... A stable IFN-λ-expressing cell line was obtained by transfection of one million Lenti-X (HEK293T, Takara) cells with 500 ng Super PiggyBac Transposase vector and 1250 ng of PiggyBac transposase mammalian expression vector using Lipofectamine 3000 following to the manufactureŕs instructions ...
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bioRxiv - Biochemistry 2022Quote: ... An oligonucleotide probe against snoRNA58 labeled with [γ-32P] ATP by T4 polynucleotide kinase (Takara, cat# 2021A) was used as a loading control ...
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bioRxiv - Immunology 2022Quote: ... using SYBR Premix Ex Taq II or TB Green Premix Ex Taq II (Takara Bio). The primer sequences used were shown in Table S5 (Tara et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Insoluble material was removed by centrifugation and the supernatant containing the receptor was rotated with Talon resin (Takara) overnight ...
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bioRxiv - Molecular Biology 2020Quote: LNA/DNA probes (PAGE grade, fasmac, Table SX) were labelled with 32P-γ-ATP by means T4PNK (Takara), followed to filter by MicroSpin G-25 Columns (GE healthcare) ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were transduced with the above-mentioned γ-retroviral SFG construct following plating on retronectin (Takara Bio)-coated plates and spinoculation ...
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bioRxiv - Cancer Biology 2019Quote: ... SYBR Premix Ex Taq II (TaKaRa) was used to perform quantitative RT-PCR in Agilent Mx3005P ...
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bioRxiv - Microbiology 2021Quote: ... SYBR Premix Ex Taq II (Takara) was used for quantitative real time PCR for quantification of target gene expression ...
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bioRxiv - Microbiology 2019Quote: ... The pCold™ II (Takara Bio) and pET50b-Amp plasmids for protein expression in E ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with SYBR Premix ExTaq II (Takara). Pre-designed primer sets for mouse TNF-α (primer set MA097070) ...
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bioRxiv - Plant Biology 2024Quote: ... SYBR Premix Ex Taq II (Takara) and the primers listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2019Quote: Human MPS1 was amplified from human testis cDNA (Marathon cDNA; Takara Bio Inc.) using Pfu polymerase (Agilent Technologies) ...
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bioRxiv - Cell Biology 2020Quote: Human CDC20 was amplified from human testis cDNA (Marathon cDNA; Takara Bio Inc.) using Pfu polymerase (Agilent Technologies) ...
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bioRxiv - Physiology 2019Quote: Human TMEM206 was cloned from cDNA obtained from a human brain cDNA library (Clontech) using the following primers:
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bioRxiv - Cell Biology 2021Quote: ... using SYBR Premix Ex Taq II (TaKaRa). The analysis of each sample in triplicate was performed more than twice for each experiment ...
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bioRxiv - Microbiology 2021Quote: ... and SYBR Premix Ex Taq II (Takara) following the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... and SYBR Premix Ex Taq II (Takara) was used for amplification according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... with SYBR Premix Ex Taq II (TaKaRa). We used specific primers ...
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bioRxiv - Cell Biology 2021Quote: ... SYBR Premix Ex Taq II (Takara, RR081A) or TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Genomics 2022Quote: ... Tissues were homogenized using BioMasher II (TaKaRa) with RLT Plus buffer (1% β-mercaptoethanol).
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bioRxiv - Plant Biology 2022Quote: ... 10 μl SYBR Premix ExTaq II (Takara), 0.4 μM gene-specific primers and 20 ng cDNA ...
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bioRxiv - Immunology 2023Quote: ... TB Green Premix Ex Taq II (TaKaRa) and StepOneplus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... using SYBR Premix Ex Taq II (Takara) in conjunction with primer sets specific to human SOD1 (hSOD1 ...
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bioRxiv - Cell Biology 2023Quote: ... using SYBR Premix Ex Taq II (Takara) reaction mix with primer sets specific to guide strand cDNA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... using SYBR Premix Ex Taq II (Takara) in conjunction with primer sets specific to mRNA of test genes and an internal control for either human (i.e. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... using SYBR Premix Ex Taq II (Takara) in conjunction with primer sets specific to cynoSOD1 and an internal control (i.e. ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were transiently transfected either with GluK3EM or co-tansfected with Wild type/mutant receptors along with GFP expressing plasmid (2 µg/dish) using Xfect reagent (Clontech). Currents were recorded from medium sized cells expressing a moderate level of fluorescence from either the fused EGFP in case of GluK3EM or co-expressed EGFP and having a capacitance of ∼5-6 pF at 48-60 hours post transfection ...