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Citations for Takara Bio :
1 - 50 of 2017 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade A UGMED5 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and tat vectors (Takara Bio Inc.). MTLn3 cells were infected with the lentivirus containing the four TC10-targeting sgRNAs or the NT1 control sgRNA and were cultured as described in the Cell Culture section ...
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bioRxiv - Molecular Biology 2024Quote: ... and the human herpes simplex virus 5 puromycin resistance marker (Clontech).
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bioRxiv - Immunology 2021Quote: ... and the NES sequence of the HIV-1 rev protein into pT2ADW vector (Komatsu et al., 2018) by In-Fusion cloning (Takara Bio).
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bioRxiv - Microbiology 2021Quote: ... HIV-1 viral stocks were titered by RT-qPCR (Takara #631235) to determine viral RNA copies / mL ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 NL4-3 Vpu ORF was cloned into pEGFP-N1 (Clontech, France). The ORF of human ATG5 was cloned in frame with HA tag into pAS1B vector (pAS1B-HA-ATG5) ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus transduction was performed using RetroNectin® Recombinant Human Fibronectin Fragment (Takara, cat.no. T100A/B) according to the manufacturer’s instructions (with centrifugation) ...
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bioRxiv - Biophysics 2022Quote: HIV-1 Gag (75) was ligated into pEGFP-N1 (Clontech, Takara Bio, Mountain View, CA) to generate the HIV-1 Gag-EGFP vector ...
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bioRxiv - Biophysics 2022Quote: HIV-1 Gag (75) was ligated into pEGFP-N1 (Clontech, Takara Bio, Mountain View, CA) to generate the HIV-1 Gag-EGFP vector ...
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bioRxiv - Molecular Biology 2021Quote: ... we performed ELISA against HIV p24 (TaKaRa Clontech #632200) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... we performed ELISA against HIV p24 (TaKaRa Clontech #632200) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing media was harvested for virus isolation using Lenti-X concentrator (Takara) in lieu of ultracentrifugation ...
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bioRxiv - Microbiology 2020Quote: ... HIV viral particles were purified using the Lenti-X concentrator (Takara) and were resuspended in fresh DMEM medium supplemented with 5% of FBS ...
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bioRxiv - Microbiology 2021Quote: The LTR promoter of the HIV-1 laboratory strain pNL4-3 was cloned into the pTA-Luc backbone (Clontech) and is henceforth referred to as pTA-Luc-NL4-3 ...
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bioRxiv - Microbiology 2023Quote: ... tat genes) (67) and 0.83 μg of pMD.G (VSV-G) expression vectors using TransIT-LT1 (Takara, Cat# MIR2306) into 293T cells ...
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bioRxiv - Immunology 2022Quote: ... T cells were infected with either replication-competent HIV-1 (pAD8gNef [80] or pNLgNef [79] or single-round HIV-1 (pHR-CD8a-d2eGFP-IRES-Nef) using Lenti-X Accelerator (TaKaRa) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... VLP were quantified by a LFA assay for HIV p24 (GoStix Plus, Takara). In this case the number of virions per volume is calculated by the approximate stoichiometry of p24 in VLP (∼2000/virion) ...
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bioRxiv - Bioengineering 2024Quote: ... VLP were quantified by a LFA assay for HIV p24 (GoStix Plus, Takara). In this case the number of virions per volume is calculated by the approximate stoichiometry of p24 in VLP (∼2000/virion) ...
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bioRxiv - Microbiology 2019Quote: ... Proviral DNA sequences of resistant HIV-1 variants were extracted from the infected into MT4 cells using NucleoSpin® Tissue (TAKARA). DNA sequences of the IN regions were amplified with 1st primers (5’-AAA TTT AAA TTA CCC ATA CAA AAG GAA ACA TGG GAA GC-3’ and 5’-GGT CTG CTA GGT CAG GGT CTA CTT GTG TGC TAT ATC-3’ ...
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bioRxiv - Developmental Biology 2022Quote: ... Virus containing supernatant was collected and virus particles were concentrated by Lenti-X concentrator (Clontech Laboratories, CA) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: ... Quantitation of pseudotyped viruses was performed using an HIV p24 assay kit (Takara Bio) or ELISA for SARS-CoV-2 S (Sino Biological ...
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bioRxiv - Microbiology 2019Quote: ... (5’-GGT CTC TCT GGT TAG ACC AGA TCT GAG C-3’ and 5’-AAA CAT GGG TAT TAC TTC TGG GCT GAA AG-3’) using PrimeSTAR®HS (TAKARA) and purified by QIAquick Gel Extraction (QIAGEN) ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR (qPCR) analysis of intracellular HIV-1 DNA levels was conducted using Premix Ex Taq (Probe qPCR) Rox plus (Takara Bio, Kusatsu, Japan). The oligonucleotides HIV-1 LTR and β2-microglobulin were used for HIV-1 DNA quantification and cell number determination ...
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bioRxiv - Pathology 2021Quote: ... Viral titering was performed using 15 μL of unconcentrated virus or 1.5 μl of concentrated virus with the Lenti-X qRT-PCR Titration Kit (Clontech, 632165). Results were read on an ABI QuantStudio 6 RT-PCR machine.
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bioRxiv - Biophysics 2022Quote: ... Virus was concentrated using Lenti-X Concentrator (Takara) and resuspended in DPBS before being applied to neurons.
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bioRxiv - Cell Biology 2022Quote: ... The virus titer (ifu/ml) defined by Clontech’s Lenti-X qRT-PCR Titration Kit (Cat ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... Virus was precipitated using Lenti X-concentrator (Takara) following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2024Quote: ... Virus was concentrated using Lenti-X Concentrator (Takara) and titer quantified using p24 ELISA antigen assay (Takara) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human specific cytoplasm (TAKARA, Stem121, 1:500), anti-TBR2 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... The virus was concentrated using Lenti-X Concentrator (Clontech). Lentivirus titers from 48h and 72h were determined by plaque assay (data not shown ...
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bioRxiv - Immunology 2019Quote: ... and virus was concentrated using Lenti-X Concentrator (Takara). Existing mIRAK1mCherry and Irak1−/− iBMDM were then transduced with concentrated lentivirus ...
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bioRxiv - Genomics 2021Quote: ... and virus was collected with LentiX Concentrator (Takara, # 631232). HUVEC were infected with combinations of two viruses and used 96 h after infection ...
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bioRxiv - Neuroscience 2021Quote: ... The virus was concentrated using Lenti-X Concentrator (Clontech), and suspended in PBS.
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bioRxiv - Microbiology 2019Quote: ... Virus packaging was assessed using Lenti-X GoStix (Clontech) but virus titers were not otherwise measured ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was extracted using Lenti-X concentrator (Clontech Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was extracted using Lenti-X concentrator (Clontech Takara) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Virus was concentrated using LentiX concentrator (Takara, Shiga, Japan).
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bioRxiv - Immunology 2023Quote: ... the virus was concentrated using RetroX concentrator reagent (Takara). Meanwhile ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus was concentrated with Lenti-XTM Concentrator from Takara and re-suspended in NeuroCultTM NS-A Complete Media.
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bioRxiv - Neuroscience 2022Quote: 50 µg of human cerebral cortex lysate (Protein Medley, Takara, Kusatsu, Japan, Cat. No. 635323) were diluted and prepared according to manufacturer’s instructions (protocol PT1602-1) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human nuclei (STEM101; Takara, Y40400, IgG1, 1:100) and anti-human NOGOA (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human specific GFAP (hGFAP, TAKARA, Stem123, 1:1000), anti-AQP4 (Cell Signaling ...
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bioRxiv - Bioengineering 2022Quote: ... Virus-containing supernatants were concentrated with Retro-X concentrator (Takara). PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus was extracted using Lenti-X™ concentrator (Clontech Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus was extracted using Lenti-X™ concentrator (Clontech Takara) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was concentrated using Lenti-X Concentrator (Clontech, 631231), resuspended by one-twentieth volume of PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Virus titers were measured using AAV titration kit (TaKaRa/Clontech) according to the manufacturer’s instructions by determining the number of DNase Iresistant vg using qPCR (StepOne ...
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bioRxiv - Neuroscience 2022Quote: ... Virus titers were measured using AAV titration kit (TaKaRa/Clontech) according to the manufacturer’s instructions by determining the number of DNase Iresistant vg using qPCR (StepOne ...
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bioRxiv - Neuroscience 2022Quote: ... The virus-containing media was concentrated with LentiX Concentrator (Takara) and resuspended in Neurobasal (Gibco).
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bioRxiv - Bioengineering 2023Quote: ... All virus was titrated with Lenti-X GoStix Plus (Takara) before being aliquoted and stored in −80 °C.
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bioRxiv - Bioengineering 2023Quote: ... Virus was titered using Lenti-X GoStix Plus (Takara Bio). Lentivirus encoding GFP was purchased by VectorBuilder (Chicago ...