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Citations for Takara Bio :
1 - 50 of 88 citations for Glutathione S transferase Class mu 26 kDa Isozyme GST Schistosoma Japonicum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged protein was eluted with 6ml glutathione (10mg/ml, Takara) and the flow-through was collected ...
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bioRxiv - Microbiology 2021Quote: ... and 10 μL (1 mU) of Pfu PGAP (TaKaRa) was added ...
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bioRxiv - Neuroscience 2021Quote: Plasmids encoding GST-BVR (GST-BVRα) and GST-BVRβ were generated by cloning cDNA of human BLVRA and BLVRB into pCMV-GST vector (Clontech/TaKaRa). The construct encoding myc-FAK was generated as previously described (95) ...
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bioRxiv - Biochemistry 2020Quote: ... Glutathione-Superflow resin (635607, Takara), His60 Ni Superflow Resin (635660 ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... or glutathione-superflow resin (Clontech) for GST tag overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and tailed with poly-A homopolymer using terminal deoxynucleotide transferase (Takara), ligated to a poly-T overhang adaptor using T4 DNA ligase (Enzymatics) ...
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bioRxiv - Plant Biology 2024Quote: ... A glutathione sepharose beads (635608, Takara)-based purification method was then used to purify the fusion protein ...
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bioRxiv - Microbiology 2022Quote: ... and A549-MxB(1-715)-MxB(26-715) cells expressing untagged MxB(1-715) and MxB(26-715) using the pLKOD-Ires-Puro vector (Clontech Takara Bio ...
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bioRxiv - Neuroscience 2021Quote: Plasmids encoding GST-BVR (GST-BVRα) and GST-BVRβ were generated by cloning cDNA of human BLVRA and BLVRB into pCMV-GST vector (Clontech/TaKaRa). The construct encoding myc-FAK was generated as previously described (95) ...
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bioRxiv - Microbiology 2020Quote: ... was placed in a glutathione super-flow column (Clontech), equilibrated with 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μl of Ligation Mix (5 mM ATP, 7U Terminal Deoxynucleotidyl Transferase (TdT) (2230B, Takara), 15 U T4 RNA Ligase High Concentration (M0437 ...
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bioRxiv - Cell Biology 2022Quote: ... GST tags were cleaved by HRV-3C Protease (Takara), which released the recombinant proteins from the beads ...
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bioRxiv - Microbiology 2022Quote: ... and A549-MxB(1-715)-MxB(26-715) cells expressing untagged MxB(1-715) and MxB(26-715) using the pLKOD-Ires-Puro vector (Clontech Takara Bio, Mountain View, United States). MeWo cells (kind gift from Graham Ogg ...
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bioRxiv - Plant Biology 2019Quote: ... 60 °C for 20 s and 72 °C for 15 s (Clontech SYBR Green Master Mix and Mx3000P qPCR Systems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pBE-S (TAKARA Bio Inc.), the expression plasmid backbone ...
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bioRxiv - Cell Biology 2023Quote: ... for GST-tagged protein or on TALON-Cobalt beads column (635507, Takara Bio) for 6XHIS-tagged protein as previously described [19,26] ...
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bioRxiv - Cell Biology 2023Quote: ... The guides were then PCR amplified for 26 cycles using the Ex Taq polymerase (Takara) protocol with the P5 forward primer mix (Takara ...
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bioRxiv - Plant Biology 2021Quote: ... The fused proteins were purified with the GST-tagged protein purification kit (Clontech, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... to encode a 3C-cleavable His6-GST-fusion using In-Fusion enzyme (Clontech, Takara), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... to encode a 3C-cleavable His6-GST-fusion using In-Fusion enzyme (Clontech, Takara), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant collected after centrifugation was incubated with pre-equilibrated glutathione superflow resin (Takara, #635607) for 2-3 hrs at 4°C on continuous shaking ...
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bioRxiv - Biochemistry 2022Quote: Mouse GTSF1 cDNA was synthesized at Twist Biosciences and cloned into pCold-GST (Takara Bio) bacterial expression vector by restriction cloning ...
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bioRxiv - Cell Biology 2020Quote: The GST-Bbs5 was expressed for 3 h at 27 °C in Escherichia coliBL21(DE3) (Takara) transformed with pGEX-4T-2-Bbs5-WT in the presence of 0.1 mM isopropyl-β-D-thiogalactopyranoside (Takara) ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was applied onto a pre-equilibrated column containing Protino Glutathione Sepharose™ 4B beads (Takara) and kept for binding for 3 hrs at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The expressed protein was purified using hexa-histidine-tag and GST-tag by Talon-resin (Clontech/Takara) or Glutathione-sepharose (GE healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... The expressed protein was purified using hexa-histidine-tag and GST-tag by Talon-resin (Clontech/Takara) or Glutathione-sepharose (GE healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... The expressed peptide was purified using hexa-histidine-tag and GST-tag by Talon-resin (Clontech/Takara) or Glutathione-sepharose (GE healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... The expressed peptide was purified using hexa-histidine-tag and GST-tag by Talon-resin (Clontech/Takara) or Glutathione-sepharose (GE healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... and 45 cycles from 10 s at 95°C to 30 s at 60°C using a TB Green Premix Ex TaqTM GC (Takara Bio, Japan).
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bioRxiv - Neuroscience 2021Quote: ... and 45 cycles from 10 s at 95°C to 30 s at 60°C using TB Green Premix Ex TaqTM GC (Takara Bio, Japan).
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bioRxiv - Biochemistry 2019Quote: ... GST and AOC3/ZAG) was amplified by PCR and cloned into the expression plasmid pLVX-Tight Puro (Clontech), which allows packaging of constructs in a lentiviral format ...
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bioRxiv - Molecular Biology 2021Quote: ... the PCR-amplified fragment was cloned into the NdeI and XhoI sites of the pCold-GST vector (TaKaRa).
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bioRxiv - Immunology 2021Quote: ... Full-length mIDO1 gene was PCR amplified from cDNA of IFN-γ-treated ID8 cell line (26) and inserted into the first cloning site in a retrovirus backbone vector (pQCXIX, Clontech-TaKaRa). A fusion gene of codon-optimized Luciferase (Luc2 ...
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bioRxiv - Immunology 2020Quote: ... S gene was cloned into pMSCV_Neo vector (TakaRa Bio, USA) that vector have no GFP or luciferase reporter gene ...
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bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing N-terminal GST tag and TEV protease recognition sequence was inserted into pCold I (Takara), followed by the insertion of a DNA fragment containing C-terminal 2×HA tag by NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Developmental Biology 2019Quote: ... Plasmids pGEX-GST and pGEX-GST-Nogo66 were used to express recombinant proteins using 0.1 mM Isopropyl β-D-1-thiogalactopyranoside induction for 1 h at 37°C in an Escherichia coli BL21 expression system and purified by Glutathione resin (Clontech).
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bioRxiv - Plant Biology 2019Quote: 6His-GST-CRK2cyto and 6His-MBP-RBOHD/C constructs for recombinant proteins were generated by using In-Fusion technology (Clontech). The coding regions of CRK2cyto (WT ...
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bioRxiv - Molecular Biology 2020Quote: ... All CTD inserts were cloned into the BamHI and NotI sites of pQLink-GST using InFusion cloning (Addgene #138470-138472) (Clontech). pQLink-GST encodes an N-terminal GST tag followed by a TEV protease cleavage site.
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bioRxiv - Biochemistry 2022Quote: ... The induction of His-and GST-fusion proteins and their subsequent purification using TALON® Metal Affinity Resin (Clontech Laboratories) and Glutathione SepharoseTM 4 Fast Flow beads (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: Clarified lysates containing 6His-GST-AVI-PLpro or 6His-GST-AVI-PLpro E912R E948R M953E Y1013K (S1* mutant) were incubated with His60 Ni Superflow Resin (TaKaRa) for 1 hr at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and Human GABRB3 (IMAGE ID 3871111, Source BioScience) were used to obtain N-terminal GST fusions in pGEX-KG (Clontech) or N-terminal FLAG fusions in pJEN1 (pcDNA3 derived ...
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bioRxiv - Pathology 2021Quote: ... Expression of the GST-N protein of SARS-CoV-2 was induced by isopropyl-D-1-thiogalactopyranoside (0.3 mM IPTG, Takara Bio). The cell pellets were sonicated ...
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bioRxiv - Microbiology 2022Quote: ... S-6P-NanoLuc proteins were released after HRV 3C protease (TaKaRa) treatment overnight at 4°C.
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bioRxiv - Immunology 2021Quote: ... Full-length mIDO1 gene was PCR amplified from cDNA of IFN-γ-treated ID8 cell line (26) and inserted into the first cloning site in a retrovirus backbone vector (pQCXIX, Clontech-TaKaRa). A fusion gene of codon-optimized Luciferase (Luc2 ...
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bioRxiv - Cell Biology 2022Quote: ... was amplified by PCR and subcloned into pBacPAK-His3-GST construct (provided by Dr. Svend Kjaer, The Francis Crick Institute) via In-Fusion cloning method (Takara Bio). StrepII2x-ATG5 ...
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bioRxiv - Biophysics 2019Quote: The resulting constructs were then transferred into pRSF-oRibo-Q1-oGST by using the GST-internal SwaI and post-H6 SpeI restriction sites and In-Fusion Cloning (Takara Bio). pRSF-oRibo-Q1-oGST was digested using SwaI and SpeI ...
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bioRxiv - Microbiology 2021Quote: ... His-Raf1, His-ATG8) and empty plasmids (GST tag, 6×His tag) were transformed into component cells Escherichia coli BL21 (Takara, 9126) or BL21 (DE3 ...
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bioRxiv - Biophysics 2019Quote: Rab8a-His6 proteins (pET19) were co-expressed with GroEL/S (pGro7, Takara) in BL21(DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by SDS–PAGE and immunoblotting with goat anti-GST (1:3000, Cytiva, 27-4577-01) and mouse anti-6xHis (1:3000, Takara Bio, 631212) antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were induced by 1 mM IPTG at 16°C for 20 h, then purified by a GST-tag Protein Purification Kit (Beyotime, Shanghai, China) or His TALON Purification Kit (Takara, Beijing, China). Corresponding primers are listed in Supplemental Table S2.