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Citations for Takara Bio :
1 - 50 of 1630 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the human cytomegalovirus (hCMV) promoter/immediate-early enhancer (IE) of peGFP-N1 (Clontech) and the chimeric intron (chI ...
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bioRxiv - Bioengineering 2022Quote: ... gene segments of HA7 and NA9 ectodomains were cloned downstream of the cytomegalovirus promoter in pTriEx3 expression vector (Clontech). The vectors were transfected into A549 cells in 96-well microtiter plate using TransLT transfection reagent (Mirus ...
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bioRxiv - Biochemistry 2020Quote: ... Each PCR product was subcloned into the NheI and XhoI sites of a cytomegalovirus promoter-driven pIRES2-AcGFP1 vector (Clontech Laboratories Inc. ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysate was mixed with SDS-PAGE loading buffer (Takara) containing 200 mM DTT ...
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bioRxiv - Microbiology 2019Quote: ... and cell lysate passed over a TALON metal affinity column (Takara). After washing the column with Buffer I ...
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bioRxiv - Genomics 2024Quote: ... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.
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bioRxiv - Systems Biology 2024Quote: ... We used 600 µl of cell lysate for total RNA extraction (Clontech 740955), followed by processing for microarray (for interactome assembly ...
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bioRxiv - Biochemistry 2023Quote: ... the clarified cell lysate was incubated with pre-equilibrated Ni- NTA resin (Takara) for 2 h at 4°C in slow shaking conditions ...
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bioRxiv - Cell Biology 2020Quote: ... target proteins were purified from cell lysate by Ni-iminodiacetic acid affinity chromatography (Clontech). However ...
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from skin or cell lysates using TRIzol Reagent (RNAiso Plus, TaKaRa). From isolated RNA ...
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bioRxiv - Biophysics 2020Quote: ... 50 µl of cell lysate was added to equal volume of LDH substrate (Takara, France) in a 96-well microplate and incubated at room temperature for 5 min ...
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bioRxiv - Bioengineering 2020Quote: ... and the DNA isolated from the cell lysates using viral DNA extraction kit (TaKaRa, Japan) was used to determine the cleavage activity of HSV-1 genomes by Sanger sequencing and TIDE analysis ...
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bioRxiv - Neuroscience 2023Quote: Small scale AAV cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were purified from the soluble cell lysate using His60 Ni Superflow Resin (Takara Bio) and concentration was measured using NanoDrop ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized and amplified from cell lysates using a SMART-Seq HT Kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Adeno-associated virus (AAV) cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
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bioRxiv - Microbiology 2023Quote: ... Genomes in the cell lysate were digested by 600U/mL of HindIII (Takara Bio, Shiga, Japan) for 4 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations of cell lysates were measured using a bicinchoninic acid protein assay kit (Takara Bio Inc.). Cell lysate containing the same amount of protein was mixed with 10 volumes of methanol and then centrifuged at 20,000 × g for 30 min to precipitate proteins ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was extracted from crushed tissue or cell lysate using the TRIzol reagent protocol (TAKARA/Thermo). cDNA was synthesized using Superscript III (Thermo) ...
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bioRxiv - Biochemistry 2023Quote: ... EPHA2- FN2 and antigen-A were purified using His 60 Ni Superflow resin (Takara), whilst antigen-B was purified with rProteinA Sepharose (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: Tissue protein lysates were purchased from Takara. Details for each lysate are shown in Table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... The cell lysate was clarified by centrifugation at 30,000 g and fractionated using His60 Ni Superflow Resin (ClonTech). The resin was washed using wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from brain tissues and cell lysates using an RNAiso Plus reagent (Takara, Shiga, Japan) and cDNA was synthesized using ReverTra Ace qPCR RT Master Mix (Toyobo ...
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bioRxiv - Pathology 2023Quote: Total RNA was extracted from skin biopsies and cell lysates using RNAiso Plus Reagent (Takara, Cat No: 9109) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was incubated with Talon Resin (CLONTECH) for 1 h at 20°C ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was incubated with Talon Resin (CLONTECH) for 1 h at 20°C ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were then added to TALON ® (TaKaRa) resin and incubated for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified lysates were incubated with HIS-60 (Takara) resin for 30-60 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Lysate was loaded on TALON resin (Takara Bio) gravity flow column ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting cell lysate was used for affinity purification and flowed over a TALON cobalt affinity resin (Takara Bio). The cobalt resin was washed with ~20 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... pellets were lysed using an SLM Aminco Pressure Cell Press and the clarified lysate was applied onto a HisTALON Superflow column (Clontech). Bound proteins were eluted by applying an increasing imidazole concentration ...
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bioRxiv - Cancer Biology 2022Quote: Telomerase-mediated extension and subsequent amplification of TRAP products were conducted in 25-µL reactions containing 1 µL of cell lysate and 2 U of Titanium Taq DNA polymerase (Takara). The other kit components—TRAP reaction buffer ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.4) and the protein contents of the cell lysate were quantified using a BCA Protein Assay Kit (TAKARA, T9300A) following the manufacturer’s protocol (Song et al. ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was obtained by centrifugation of the cell lysate at 10,000 g for 30 minutes and applied into the His TALON™ gravity column (Clontech). The columns were washed to remove the non-target proteins ...
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bioRxiv - Biochemistry 2019Quote: ... Cleared lysates were incubated with TALON resin (Clontech, 635504) which was preequilibrated with 50 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was incubated with TALON metal affinity resin (Takara) (3ml slurry per L culture ...
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bioRxiv - Microbiology 2020Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Monocytes were infected immediately after purification by adding HIV-1BaL virus to the culture at MOI between 3 and 5 based on the p24 titer ...
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bioRxiv - Immunology 2020Quote: Levels of p24 antigen in the purified SARS-CoV-2 pseudotype solution was measured by ELISA (Takara). Mouse sera were heat inactivated ...
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bioRxiv - Developmental Biology 2020Quote: ... tetracycline-controlled transactivator (tTA) and the SV40 large T antigen polyadenylation signal (Takara Bio, Mountain View, CA), into B6SJLF1 fertilized eggs ...
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bioRxiv - Microbiology 2022Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Infections of fully differentiated MDM were performed after adherent growth in the presence of M-CSF for seven days ...
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bioRxiv - Microbiology 2021Quote: ... Clarified lysates were loaded onto a 1 mL TALON (Takara) column ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was then incubated with 25μL NiNTA beads (Clontech) for 1 hour at 4°C for binding ...
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bioRxiv - Biochemistry 2020Quote: Clarified lysate was incubated with His60 Ni Superflow Resin (TaKaRa) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cleared lysate was subjected to purification using TALON resin (Clontech) which was equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: Clarified lysate was incubated with His60 Ni Superflow Resin (TaKaRa) for 1 hr at 4°C ...
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bioRxiv - Biochemistry 2020Quote: Clarified lysate was incubated with His60 Ni Superflow Resin (TaKaRa). Resin was washed with a buffer (300 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was added to TALON CellThru Resin (Takara) and incubated at 4°C for 1h ...
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bioRxiv - Genomics 2024Quote: ... Lysates were incubated with His60 Ni Superflow resin (Takara Bio) at 4°C with rotation for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were prepared using a French press and susequent protein purification accomplished using Talon metal affinity resin (TaKaRa Bio USA, Inc.) as described by the manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... CST was purified from the clarified lysate using Talon resin (Clontech) followed by protease cleavage of the GFP-His10 tag and finished with size exclusion chromatography on a Superdex 200 column equilibrated in Buffer A (25 mM HEPES pH 7.5 ...