Labshake search
Citations for Takara Bio :
1 - 50 of 458 citations for Calcium Channel Voltage Dependent L Type Alpha 1C Subunit CACNA1C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... mCherry-tagged human SREBP-1c was inserted into pmCherry (mCherry-SREBP-1c) (Clontech), and HA-tagged hamster SCAP ...
-
bioRxiv - Genomics 2023Quote: ... using calcium-phosphate (Takara). Media was changed at 24 hours and samples were collected 72 hours post-transfection.
-
bioRxiv - Cell Biology 2020Quote: ... 68℃ for 1min (Fig. 1C) or using EmeraldAmp GT PCR Master Mix (Takara Bio) for 30 cycles of 98℃ for 10s ...
-
bioRxiv - Cell Biology 2019Quote: ... Calcium phosphate transfection kit (Takara) was used for transfection ...
-
bioRxiv - Neuroscience 2020Quote: ... a Calcium Phosphate Transfection kit (Clontech) was used to transfect cells with a mixture of OXTR-iTango2 DNA plasmid vectors with a secreted alkaline phosphatase (SEAP ...
-
bioRxiv - Bioengineering 2022Quote: ... were transiently transfected using calcium phosphate (Takara) and retrovirus-containing supernatants were collected 2 days later ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 2021: Escherichia coli DH5 alpha (Takara (Korea)) ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK-293T cells were transfected with calcium phosphate (Clontech) in T75 flasks using a total of 30 μg plasmid DNA ...
-
bioRxiv - Biophysics 2021Quote: ... using the calcium phosphate method (Takara Bio, Shiga, Japan). All animal experiments complied with the protocols approved by the Institutional Animal Care and Use Committee of Tohoku University (2016EgA-003 ...
-
bioRxiv - Microbiology 2019Quote: The dual-luciferase assay using an NF-□B–dependent firefly luciferase (pNF-□B-Luc; Clontech) and a Renilla luciferase driven by the thymidine kinase promoter (pRLTK ...
-
bioRxiv - Cell Biology 2019Quote: ... HEK-293 T cells were transfected with calcium phosphate (CalPhos, Clontech) in T75 flasks using 30 μg of plasmid DNA ...
-
bioRxiv - Immunology 2021Quote: ... pMD2.G and the CAR-expression plasmid by calcium phosphate transfection (Clontech). At 16 hrs post-transfection cells were washed with PBS and incubated in complete DMEM media containing 6 mM sodium butyrate (Sigma Aldrich) ...
-
bioRxiv - Genomics 2021Quote: ... by calcium phosphate transfection with CalPhos™ Mammalian Transfection Kit (Takara, 631312) following the manufacturer’s protocol into HEK293T cells ...
-
bioRxiv - Microbiology 2024Quote: ... The HA1 subunit was divided into three fragments for PCR (PrimeSTAR Max DNA Polymerase, Takara Bio), seven Ns and partial sequencing adapters were added in the primer as the barcode ...
-
bioRxiv - Cell Biology 2023Quote: ... procollagen type I (Takara #MK101), fibronectin (Takara #MK115) ...
-
bioRxiv - Neuroscience 2019Quote: ... The calcium phosphate-based CalPhos Mammalian Transfection Kit (631312, Clontech Laboratories, Mountain View, CA) was used (Harata et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... red fluorescent protein fused with the mitochondrial targeting sequence from cytochrome c oxidase subunit VIII (Clontech, Mountain View, CA) using Lipofectamine 2000 (Life Technologies ...
-
bioRxiv - Developmental Biology 2019Quote: ... 3Flag-BMAL1 and 3HA-CNOT1-C were transfected by the calcium phosphate-based kit (Clontech) in 10cm plates of HEK293T cell cultures ...
-
bioRxiv - Cell Biology 2021Quote: ... and the respective retroviral vector (pWPI) using calcium phosphate transfection (CalPhos Mammalian Transfection Kit, Takara Bio Europe ...
-
bioRxiv - Neuroscience 2019Quote: ... using the calcium phosphate-based CalPhos Mammalian Transfection Kit (631312, Clontech Laboratories, Mountain View, CA) (Harata et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... as internal control by the calcium phosphate transfection method according to the manufacturer’s protocol (Takara). Raji cells (3×106cells ...
-
bioRxiv - Bioengineering 2023Quote: ... 661W cells were transfected with the calcium phosphate technique or the XfectTM transfection reagent (Takara). MEF cells were transfected using TurboFectTM Transfection Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... X-alpha-Gal (X-α-gal) and aureobasidin A (AbA) were obtained from TaKaRa. Plasmids ...
-
bioRxiv - Immunology 2022Quote: ... – PU6-pAzpa was co-transfected with plasmids expressing full-lengths mutant 2B4 TCR and CD3 subunits using Xfect transfection kit (TaKaRa). For incorporating pAzpa into TCR sites ...
-
bioRxiv - Neuroscience 2024Quote: ... HEK293 cells in 24-well plates were transfected with a total of 0.9 μg of DNA vectors carrying genes encoding GluN1 and GluN2 subunits and green fluorescent protein (GFP; pQBI 25 vector; for identification of transfected cells; Takara) in a 1:1:1 ratio using 0.9 μl of PolyMag reagent (OZBiosciences ...
-
bioRxiv - Neuroscience 2024Quote: ... was amplified by PCR and inserted into the Cre-dependent AAV hSyn FLEx vector using BamHI/KpnI restriction sites.pTet-on (transactivator) was purchased from Clontech (Clontech; #P3070-5). Plasmids were prepared using the ZymoPURE Plasmid MaxiPrep Kit (Zymo Research ...
-
bioRxiv - Neuroscience 2020Quote: ... in a 100 mm dish using a calcium phosphate transfection kit (CalPhos Mammalian Transfection kit, Clontech, 631312). Two days post-transfection ...
-
bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were transfected at 11 days in vitro (DIV 11) using Calphos calcium phosphate transfection reagent (Takara) according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... and the psPAX2 (6.75 μg) vectors by the calcium phosphate transfection method according to the manufacturer’s protocol (Takara). 72 hours post-transfection ...
-
bioRxiv - Neuroscience 2019Quote: ... and doxycycline 2g/L (Clontech). On day 3 ...
-
bioRxiv - Neuroscience 2022Quote: ... Doxycycline (2 mg/L, Clontech) was also included on d0 to induce TetO gene expression by binding to rtTA and the TetO promoter upstream of the Ngn2 gene ...
-
bioRxiv - Cell Biology 2019Quote: ... wild-type SAF-A was cloned into pEGFP-N1 (Clontech) and confirmed by DNA sequencing ...
-
bioRxiv - Molecular Biology 2020Quote: ... and the psPAX2 (6.75 μg) packaging vectors by the calcium phosphate transfection method according to the manufacturer’s protocol (TaKaRa; Calphos). 72h post-transfection ...
-
bioRxiv - Cancer Biology 2023Quote: ... Individual lentiCRISPRv2 vectors were introduced along with packaging vectors into human embryonic kidney (HEK) 293T cells via calcium phosphate transfection according to the manufacturer’s protocol (Clontech). Lentivirus was harvested at 48 and 72 hours after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters prior to transduction of cancer cell lines using centrifugation at 1000 g for 2 hours in the presence of 8 μg/mL of polybrene (Santa Cruz Biotechnology) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The α1-subunit genes were inserted at the PH promoter of vectors already containing the corresponding β1-subunit proteins using In-Fusion® HD Cloning Kit (Takara Bio, USA Inc.) and control sequenced ...
-
bioRxiv - Neuroscience 2021Quote: ... The cDNAs encoding full-length human HCN1 channel and mouse TRIP8b (splicing variant 1a-4) were cloned into the pcDNA 3.1 (Clontech Laboratories) mammalian expression vector ...
-
bioRxiv - Microbiology 2021Quote: ... retroviral packaging construct pTG5349 and a reporter pCCLSIN.cPPT.hPGK.GFP.WPRE into HEK 293T cells by calcium-phosphate method (Calphos Mammalian Transfection kit, Clontech as per manufacturer’s instructions). Cells were seeded on 60×15mm dish a day before transfection to achieve 70 – 80% confluency ...
-
bioRxiv - Cell Biology 2021Quote: Transfection of Halo-tagged AnkB440 plasmids were conducted in HEK293T cells grown in 10 cm culture plates using the calcium phosphate transfection kit (Takara) and 8 µg of plasmid ...
-
bioRxiv - Immunology 2022Quote: ... or the expression vector LentiCRISPRv2 (coding for guideRNA) in a 3:1:3 ratio for 8 hours using calcium phosphate transfection (CalPhos Mammalian Transfection Kit, Takara Bio Europe ...
-
bioRxiv - Cancer Biology 2023Quote: ... lentiviral constructs pCMV-VSV-G and pCMV-dR8.2 dvpr 98 were transfected in HEK293T cells by the calcium-phosphate method (CalPhos Mammalian Transfection Kit, Takara, 631312) for virus production ...
-
bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected with 1 µg of TRPC6 channel plasmids (WT, T221A and 112Q) using Xfect transfection reagent according to manufacturer’s protocol (Takara Bio 631318). Cells expressing wild type and mutant channels were identified by YFP fluorescence ...
-
bioRxiv - Cell Biology 2022Quote: ... The extracted probes were radioactively labeled with 2.5 μl [alpha-32P] dCTP (Amersham) according to the instructions of the kit (LaddermanTM Labeling Kit, Takara). The probe was mixed with 100 μl TE buffer (10mM ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were transfected with plasmids expressing either SARS-COV-2 S protein WT or mutants (E1182K, L1193G, E1182K/L1193G, L1182K/L1186G/L1193G) by using Calcium phosphate transfection kit (Takara-Bio). 24 h post transfection ...
-
bioRxiv - Cancer Biology 2023Quote: Production of vesicular stomatitis virus (VSV-G) pseudotyped lentivirus was performed by calcium phosphate transfection of Lenti-XTM 293T cells (TaKaRa Clontech, 632180) with CROP-mCherry and helper plasmids pMD2.G and psPAX2 (Addgene plasmids 12259 and 12260) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and wild type etoll-9 was cloned in pGBKT7-BD vector (Clontech). Each mutant etoll-AD was transformed in Y187 strain followed by selection on synthetic defined (SD ...
-
bioRxiv - Genomics 2019Quote: ... Wild-type sequences were generated by PCR using human genomic DNA (Clontech) as a template ...
-
bioRxiv - Developmental Biology 2023Quote: ... Plasma Gla-type osteocalcin was analyzed by enzyme immunoassay (#MK111, Takara, Japan). The absorbance was read at 450 nm using a microplate reader (Bio-Tek ...
-
bioRxiv - Biochemistry 2020Quote: One colony was used to inoculate 10mL of selection media (6.9 g/L Yeast Nitrogen Base without amino acids, 0.62g/L Clontech -Leu/-Trp/-Ura dropout supplement ...
-
bioRxiv - Biochemistry 2020Quote: ... Transformed cells were plated on a selection agar (6.9g/L yeast nitrogen base without amino acids, 0.62g/L Clontech -Leu/-Trp/-Ura dropout supplement ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Neurons were transfected with GCaMP6 or NEMO-encoding plasmids at 7 to 9 days after plating using a calcium phosphate transfection kit (Takara Bio Inc).