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Citations for Takara Bio :
1 - 50 of 53 citations for CRISPR Screening since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... together with linearized screening puromycin marker (Clontech). Two days post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... the Lenti-X CRISPR/Cas9-System (Takara) was used ...
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bioRxiv - Molecular Biology 2022Quote: Y1H library screening was performed by using the Matchmaker Gold Yeast One-Hybrid Library and Screening kit (Clontech,CA, USA). The promoter fragments of CaPR1 were inserted in the pAbAi vectors ...
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bioRxiv - Physiology 2023Quote: ... was validated in vitro (sgRNA screening system, Clontech), and obtained from PNAbio ...
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bioRxiv - Neuroscience 2019Quote: Y2H screening was performed using the Matchmaker Gold System (Clontech). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Gal4-based two-hybrid screening against the mouse cDNA library (Clontech) was performed as described before (Mitsuzawa et al. ...
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bioRxiv - Cell Biology 2020Quote: ... The two plasmids for CRISPR/Cas9 were midi-prepped (Takara Bio) and co-injected by BestGene ...
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bioRxiv - Cell Biology 2023Quote: Yeast-two-hybrid library screening was performed using the MATCHMAKER system (Takara) with a human kidney yeast-two-hybrid cDNA library ...
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bioRxiv - Genetics 2023Quote: ... Initially the Matchmaker® Gold Yeast One-Hybrid Library Screening System (Takara Bio) was employed ...
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bioRxiv - Immunology 2021Quote: CRT-null HEK293T cells were generated using Guide-it CRISPR/Cas9 System (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Guide-it In Vitro Transcription and Screening Kit (Takara Bio USA, Mountain View, California) was used ...
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bioRxiv - Biochemistry 2020Quote: ... the cells were harvested for analysis using Guide-it™ sgRNA Screening Kit (TaKaRa). Stable I2PP2A knockdown CRISPR I2PP2A HEK293 cells were also maintained in MEM with 10% FBS supplemented with Gentamycin (50 mg/ml).
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bioRxiv - Plant Biology 2020Quote: Y1H assay was performed using Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The nucleotide sequences of promoter region of selected key defense genes having potential RAV1 binding motifs were retrieved by using online tool (https://bioinformatics.psb.ugent.be/plaza/) ...
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bioRxiv - Plant Biology 2023Quote: ... and Y1H analysis was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid screening was performed using the Matchmaker Gold Yeast Two-Hybrid System (Takara). The cDNA fragments corresponding to CEPDL2 and GrxS5 were subcloned into the pGBKT7 vector ...
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bioRxiv - Neuroscience 2020Quote: ... a Y2H screening was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... a Y2H screening was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Screening of interaction clones was performed as described by the manufacturer’s instructions (Clontech, TaKaRa, Dalian, China). The screened transformants from the cDNA library were transferred to grow on the stringent dropout medium which lack of Tryptophan (Trp) ...
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bioRxiv - Molecular Biology 2021Quote: ... Screening of interaction clones was performed as described by the manufacturer’s instructions (Clontech, TaKaRa, Dalian, China). The screened transformants from the cDNA library were transferred to grow on the stringent dropout medium which lack of Tryptophan (Trp) ...
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bioRxiv - Molecular Biology 2022Quote: Yeast two-hybrid screening was performed using the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) as described by the manufacturer (Young ...
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bioRxiv - Molecular Biology 2023Quote: Y1H assays were performed using the Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The sequence of LhWRKY44 was inserted into the vector pGADT7 as prey ...
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bioRxiv - Molecular Biology 2022Quote: ... we used the Matchmaker® Gold Yeast One-Hybrid Library Screening System (Clontech, Mountain View, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Y2H assays were performed as recommended in the BD Matchmaker Library Construction and Screening kit (Clontech, USA). The complete coding sequence of PRH1 was fused to the GAL4 DNA binding domain (BD ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNAs efficiency was assessed in vitro using the sgRNA In vitro Transcription and Screening kit (Clontech, 631439) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: The sgRNA sequences were amplified using Guide-it™ CRISPR Genome-Wide Library PCR Kit (Takara, 632651) and subjected to the high-throughput amplicon sequencing on NextSeq500 ...
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bioRxiv - Cell Biology 2023Quote: The lenti-CRISPR v2 constructs containing specific sgRNAs were transfected into Lenti-X 293T cells (Takara; 632180) with psPAX2 and VSV-G plasmids using the FuGENE HD transfection reagent (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... was produced and purified according to the Guide-it CRISPR/Cas9 Gesicle Production System protocol (Takara Bio) involving oligo-DNA pair annealing in a thermal cycler.
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bioRxiv - Cell Biology 2021Quote: ... The yeast mating experiment was performed as per the Two-hybrid library screening protocol for yeast mating (Clontech). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Yeast 2-hybrid library screening was conducted using the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... MC3T3-E1 and ATDC5 cells were infected with CRISPR lentivirus and selected with puromycin (4.5 μg/ml, Clontech) for 7 days ...
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bioRxiv - Plant Biology 2020Quote: Y2H screening was performed using Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA, Inc., Mountain View, US-CA). For bait AP_SAP11-like_PM19 or AP_SAP11-like_PM19Δ40-56 was cloned into pGBKT7 (TRP1 nutritional marker ...
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bioRxiv - Cancer Biology 2020Quote: The in vitro targeting efficiency of sgRNAs was tested using a Guide-it Complete sgRNA Screening System (Takara, 632636) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The sgRNAs were prepared following the instruction (Guide-it™ sgRNA In Vitro Transcription and Screening Systems, TaKaRa, Japan). Briefly ...
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bioRxiv - Neuroscience 2021Quote: Genomic clones for the Sipa1l1 locus were isolated by screening with a C57BL/6N male liver genomic library (Clontech). The targeting vector was constructed by inserting a nuclear localization signal-LacZ-polyA cassette ...
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bioRxiv - Plant Biology 2022Quote: Yeast-two-hybrid library screening was performed according to the Matchmaker Gold Yeast Two-Hybrid System User Manual (Clontech).
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bioRxiv - Developmental Biology 2021Quote: Two-hybrid screening and assays were performed as described in the Matchmaker™ Pretransformed Libraries User Manual (Takara PT3183-1) using a Drosophila Mate & Plate™ Library - Universal Drosophila (Normalized A+ RNA isolated from embryo (~20 hr) ...
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bioRxiv - Molecular Biology 2021Quote: ... The Y1H experiment was carried out according to the manufacturer’s protocol for the Matchmaker Gold Y1H library screening system (Clontech, USA). The primers are listed in Table S1.
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bioRxiv - Plant Biology 2022Quote: ... PtoATPE and PtoPSBB) were co-transformed into the Y1HGold yeast strain using the Matchmaker One-Hybrid Library Construction and Screening Kit (Clontech), respectively ...
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bioRxiv - Genomics 2019Quote: SiGRF1 protein interactions were investigated by screening a foxtail millet cDNA library in yeast with the Matchmaker Two-Hybrid System 3 (Clontech). SiGRF1 cDNA without the termination codon was cloned with EcoR1 technology in pGBKT7 ...
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bioRxiv - Microbiology 2022Quote: ... A tomato Mate & Plate library was constructed and used for screening with the Matchmaker Gold Yeast Two-Hybrid System (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... the target site of the CRISPR/Cas9 system on dnmt3aa gene was amplified using TaKaRa Ex Taq® (Takara Bio) with the primers listed in Supplementary Table S2 ...
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bioRxiv - Plant Biology 2020Quote: ... The yeast one-hybrid (Y1H) assay was conducted using the Matchmaker™ Gold Yeast One-Hybrid Library Screening System kit (Cat. no. 630491, Clontech).
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bioRxiv - Plant Biology 2022Quote: ... The recombinant constructs was transformed into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant plasmid was used as bait to screen the cDNA library using the Matchmaker™ Gold Yeast Two-Hybrid Library Screening System kit (Cat. no. 630489, Clontech).
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bioRxiv - Microbiology 2022Quote: The yeast-two-hybrid library screening was performed according to the protocols of the Matchmaker® Gold Yeast Two-Hybrid System (TaKaRa). Briefly ...
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bioRxiv - Plant Biology 2019Quote: Y1H assays were carried out using the Matchmaker Gold yeast one hybrid Library Screening System (Biosciences Clontech, Palo Alto, CA, USA). The PRH1 promoter was inserted into the pAbAi vector ...
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bioRxiv - Neuroscience 2020Quote: ... this was followed by a second screening using the SD quadruple dropout (Leu−, Trp−, Ade−, and His−) selective medium (Clontech Takara Bio). After the elimination of duplicates containing the same AD/library plasmid via yeast-colony PCR ...
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bioRxiv - Microbiology 2020Quote: ... the in vitro Cas9 cleavage was performed using a previously amplified target pyrG amplicon following the steps of the Guide-it™ sgRNA In Vitro Transcription and Screening Systems User Manual (Takara Bio USA ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant constructs were introduced into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... this was followed by a second screening using the SD quadruple dropout (Leu−, Trp−, Ade−, and His−) selective medium (Clontech Takara Bio). After the elimination of duplicates containing the same AD/library plasmid via yeast-colony PCR ...