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Citations for Takara Bio :
1 - 50 of 763 citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... in the T/C-28a2 chondrocyte cell after transfection with a pIRESpuro2 plasmid construct (Clontech) containing the human Cx43 sequence ...
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bioRxiv - Cancer Biology 2019Quote: ... then subcloned into T-clone vector pMD19-T (TaKaRa) to generate pT-DMP-2 ...
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bioRxiv - Cancer Biology 2019Quote: ... then subcloned into T-clone vector pMD19-T (TaKaRa) to generate pT-DMP-1 ...
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bioRxiv - Developmental Biology 2022Quote: c-Kit-EGFP fusion protein: chicken c-Kit cDNA was inserted into multiple cloning site of pEGFP-N1 Vector (Clontech, #6085-1) including EGFP sequences using a primer set ...
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bioRxiv - Biochemistry 2023Quote: ... CBS proteins with a permanent C-terminal His-tag were purified using TALON (Clontech) resin followed by anion exchange using a Hitrap Q column (Cytiva) ...
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bioRxiv - Cell Biology 2020Quote: Lenti-X 293 T (Clontech), Neuro2A (mouse neuroblastoma ...
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bioRxiv - Cell Biology 2022Quote: ... and oligo(T)18 primers (Takara). Relative transcript amounts were measured by SYBR-green quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into pMD18-T (Takara) vector and then transformed into E.coli DH5α for sequencing ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... and oligo(T)18 primers (Takara). ARL3 cDNA and cDNAs encoding the six BBS proteins BBS1 ...
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bioRxiv - Microbiology 2021Quote: ... and inserted to pMD19-T (TAKARA) to obtain pMD19-T-MMP0852/0853 (Table S1) ...
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bioRxiv - Microbiology 2019Quote: ... and inserted to pMD19-T (TAKARA) to obtain pMD19-T-Pmcr-tetR-Tmcr (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2020Quote: ... the amplified PCR products were cloned into a T/A cloning vector PMD18-T (TaKaRa, Tokyo, Japan), and later excised from PMD18-T plasmid using NotI and XhoI restriction sites ...
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bioRxiv - Cancer Biology 2020Quote: ... was cloned into pMD19-T simple (TAKARA) to obtain pMD19-T-DMP firstly ...
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bioRxiv - Microbiology 2021Quote: ... Lenti-X 293 T cells (Takara Bio) grown in 10 cm dish were transiently transfected with the following plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... cloned into T-Vector pMD20 (Takara, 3270), and sequenced (Thermo ...
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bioRxiv - Developmental Biology 2022Quote: ... The expression of Dunk protein was confirmed by Western blot using c-Myc Monoclonal Antibody (Takara, Cat# 631206). Then ...
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bioRxiv - Cell Biology 2021Quote: ... and inserted into T-Vector pMD20 (Takara Bio) using DNA Ligation Kit
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into a pMD19-T vector (Takara). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... and cloned into pMD18-T vector (TaKaRa, China) and the recombinant plasmids were picked and sent to Tsingke Biotechnology Co. ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were transferred to retronectin-coated (Takara) plates previously spinoculated with retroviral particles at 2000ξg for 1.5h ...
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bioRxiv - Plant Biology 2023Quote: ... 0.05% T-20 and protease inhibitor cocktail (Takara)] ...
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bioRxiv - Plant Biology 2024Quote: ... The pMD18-T vector was procured from TaKaRa Bioengineering (Dalian ...
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bioRxiv - Cell Biology 2021Quote: ... red fluorescent protein fused with the mitochondrial targeting sequence from cytochrome c oxidase subunit VIII (Clontech, Mountain View, CA) using Lipofectamine 2000 (Life Technologies ...
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bioRxiv - Plant Biology 2019Quote: ... and the Gal4 AD fused with SV40 large T-antigen (pGADT7-T) from the Matchmaker Gold Yeast Two-Hybrid System kit (Takara Bio) were used as positive control ...
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bioRxiv - Plant Biology 2019Quote: 6His-GST-CRK2cyto and 6His-MBP-RBOHD/C constructs for recombinant proteins were generated by using In-Fusion technology (Clontech). The coding regions of CRK2cyto (WT ...
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bioRxiv - Microbiology 2022Quote: ... PreS2-LHBS sequence (deletion nucleotides 2-55) was cloned into the protein stability construct after the c-terminal of EGFP by In-Fusion cloning (Takara). The protein stability construct was a kind gift from Dr ...
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bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged protein fraction was incubated for thirty minutes at 4°C with Talon resin (Clontech Laboratories/Takara Bio USA) that had been previously equilibrated in lysis/extraction buffer ...
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bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged protein fraction was incubated for thirty minutes at 4°C with Talon resin (Clontech Laboratories/Takara Bio USA) that had been previously equilibrated in lysis/extraction buffer ...
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bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing CDS of each Hero protein and C-terminal FLAG and His tags was inserted into pCold I (Takara) by NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Biophysics 2022Quote: ... These plasmids were designed by fusing the coding sequences of the respective proteins C-terminal (intracellularly) via a linker to mEGFP (called D0 / Donor only) or mCherry in the pIRESpuro2 vector (Clontech)32 (for more linker details see Supplementary Table 5) ...
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bioRxiv - Microbiology 2019Quote: ... The amplified fragments were cloned into pMD18-T (TaKaRa), confirmed by sequence analysis ...
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bioRxiv - Immunology 2019Quote: ... Connected DNA fragment to pMDTM18-T vector (TaKaRa 6011) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... The cloning vector pMD20-T was purchased from Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into T-vector pMD20 (Takara Bio Inc.). To amplify Vero-PVR1 ...
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bioRxiv - Neuroscience 2022Quote: ... and the AAVpro 293 T cell line (#632273, Takara) following the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: mRNA was captured using poly-T coated dishes (Clontech) and used for library construction using SMARTer technology (SMARTer Stranded RNA-seq Kit ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA was captured using poly-T coated dishes (Clontech) and SMARTer technology was used for library construction (SMARTer Stranded RNA-seq Kit).
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bioRxiv - Cell Biology 2023Quote: ... Clone and sequence using the pMD18-T vector (TaKara).
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bioRxiv - Bioengineering 2023Quote: ... murine T cells were transferred to retronectin-coated (Takara) plates previously spinoculated with retroviral particles at 2000ξg for 1.5h ...
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bioRxiv - Immunology 2022Quote: Transduction of T cells was performed with Retronectin (Takara) as previously described (22 ...
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bioRxiv - Immunology 2023Quote: ... T cells were transduced by centrifugation on Retronectin (Takara)-coated plates.
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bioRxiv - Neuroscience 2022Quote: ... protein lysate was immunoprecipitated for 2 h at 4°C with the following antibodies: mouse anti-GFP (1:1000; Takara Bio), mouse anti-FLAG (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Co-IPs were carried out by incubating the samples with 30 μL of protein A agarose bead slurry for 4h at 4°C in a rotating wheel and with anti-mCherry (Takara 632496) of 1:1000 dilution ...
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bioRxiv - Evolutionary Biology 2019Quote: ... was designed aimed to amplify the A+T-rich region and the fragment was ligated into pMD19-T vector (TaKaRa Bio, Otsu, Shiga, Japan). All of them were stained with ethidium bromide ...
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bioRxiv - Molecular Biology 2019Quote: ... ligated into the pMD™19-T plasmid vector (Takara), and transformed chemically into Escherichia coli competent cells ...
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bioRxiv - Cell Biology 2021Quote: Human embryonic kidney 293 T cells (HEK293T, Takara, Cat# 632180) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Genomics 2020Quote: ... Amplicons were ligated to T-Vector pMD19 (Takara, No.3271) with T4 ligase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified DNA was directly ligated with pMD18-T vector (Takara) for TA cloning according to the pMD18-T kit protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloned into pMD19-T vector (Takara Bio, Dalian, China) before transformation into E ...
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bioRxiv - Developmental Biology 2024Quote: ... the C-terminal twin strep tag was removed by incubating with the 3C protease (Takara Bio, Japan; 1.5 unit/50 mg protein) and ran on a size-exclusion column ...