Labshake search
Citations for Takara Bio :
1 - 50 of 5261 citations for Anti FLAG M5 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2022Quote: ... After digestion by BamHI and dephosphorylation treatment using SAP (TaKaRa), the DNA fragment was ligated with 1.5-2.5 kbp fragments of the Sau3AI-digested S ...
-
bioRxiv - Microbiology 2020Quote: ... anti-Flag (Clontech, USA) and anti-GFP (Sigma ...
-
bioRxiv - Neuroscience 2020Quote: ... CyRFP excised with NheI and NotI from pCAG-CyRFP was blunted with SAP (Takara: 2660A) and Klenow (Takara ...
-
bioRxiv - Cell Biology 2019Quote: ... SMAD2 #3122 and SMAD3 #9523 antibodies (Cell Signalling Technology) and anti-FLAG antibody (Clontech). Horseradish peroxidase-conjugated secondary antibodies and anti-rabbit and anti-mouse antibodies were acquired from Molecular Probes ...
-
bioRxiv - Molecular Biology 2023Quote: ... Signals for eGFP and FLAG were detected by using Mouse Anti-GFP JL-8 (Clontech), Mouse Anti-Flag (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... The mutant 2X FLAG TANGO2 Gly154Arg was generated with the In-Fusion HD Cloning Plus Kit (Takara Bio Europe) following the manufacturer’s instructions using the pMK-T plasmid as template ...
-
bioRxiv - Microbiology 2024Quote: ... pseudotyped with LUJV GPC were produced by transfecting retroviral transfer vector pLXIN-Luc encoding luciferase as a reporter gene together with LUJV-flag or mutated LUJV-flag in pcDNA3.1 into the GP2-293 retroviral packaging cell line (Clontech). GP2-293 cells were seeded at 5×106 on 10-cm plates and transfected 24 h later with 5 µg of LUJV-flag/LUJV-flag-mut and 5 µg Luciferase using Lipofectamine 2000 (Invitrogen) ...
-
bioRxiv - Developmental Biology 2019Quote: ... plasmid after digestion of the inserts by EcoRI and KpnI and cloning into the EcoRI and EcoRV sites in frame with the C-terminal Flag tag in pSNAPf plasmid using In-Fusion Kit (Clontech). The human HSF2-YFP was constructed by PCR and cloned into the XhoI and SalI sites in frame with the N-terminal YFP tag in EGFp-C1 plasmid using In-Fusion Kit (Clontech) ...
-
bioRxiv - Developmental Biology 2020Quote: ... 5′UTR of test genes and EGFP were inserted into pCAG-FLAG-N1 plasmid 48 using In-Fusion HD Cloning Kit (TAKARA).
-
bioRxiv - Molecular Biology 2020Quote: ... The amplified DNAs were subcloned into the pCK-FLAG vector (CMV promoter-driven vector) at the BamHI and XhoI sites using In-Fusion HD Cloning Kit (Clontech). For dsRBD-deleted DICER ...
-
bioRxiv - Molecular Biology 2022Quote: ... Amplified mTurbo and FLAG-cyp40 fragments were introduced into XbaI site of pUASp-K10-attB vector using In-Fusion HD Cloning Kit (Takara). To generate UASp-mTurbo-FLAG-cyp40deltaTPR ...
-
bioRxiv - Cell Biology 2022Quote: ... Lenti-X HEK293T cells were transfected with either pLVX-NME6Myc-Flag or pLVX-NME6H137N Myc-Flag using Lenti-X Packaging Single Shots (Takara). The viral supernatant was collected after 48 h ...
-
Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... stably expressing PQLC2-FLAG (pLVX-puro vector, Clontech). Proteins bound to glutathione resin were then separated by SDS-PAGE and immunoblotted with anti-FLAG antibodies while the GST-tagged proteins were visualized by Ponceau S staining (APACOR) ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR products were inserted into pLEX-FLAG lentiviral vector between the NotI and XhoI sites using the InFusion HD cloning kit (Takara Bio) to generate constructs with N-terminal FLAG tags ...
-
Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: ... The expression plasmids for circSPNS1 was constructed by subcloning the PCR-amplified fragments into FLAG-pcDNA3 plasmid using an In-Fusion HD cloning kit (Takara Bio) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2019Quote: ... pEF-Tak Flag-Riplet was generated by InFusion (ClonTech) cloning of pCAGGS-HA-Riplet into pEF-Tak ...
-
bioRxiv - Immunology 2023Quote: ... pEFTak FLAG-murineMAVS was cloned using InFusion cloning (Takara) of a PCR fragment of the MAVS open reading frame amplified from NIH3T3 cell cDNA and NotI- and PmeI-digested pEFTak FLAG vector ...
-
bioRxiv - Cancer Biology 2021Quote: ... and the oligonucleotides were inserted into C terminal of ARL4C-EGFP or ARL4C-FLAG-HA using In-Fusion HD Cloning Kit (Takara Bio Inc.).
-
bioRxiv - Microbiology 2020Quote: ... the target genes with or without glycosite mutation were cloned into the pTOR::FLAG vector using the In-fusion HD Cloning kit (Clontech, Beijing, China) (Table S1) ...
-
bioRxiv - Cell Biology 2022Quote: ... The PCR products were subcloned into the pcDNA5 FRT/TO HA FLAG Ms Jaw1 (digested with HpaI/SphI) by double-insert cloning using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO HA FLAG Ms Jaw1 504–508A ...
-
bioRxiv - Cell Biology 2022Quote: ... The PCR products were subcloned into the pcDNA5 FRT/TO HA FLAG Ms Jaw1 (digested with HpaI/BamHI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan). Similarly ...
-
bioRxiv - Cell Biology 2022Quote: ... and the DNA fragments were ligated to the pcDNA5 FRT/TO HA FLAG vector (digested with the same enzymes) using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO HA FLAG Hu sec11a and pcDNA5 FRT/TO HA FLAG Hu sec11c ...
-
bioRxiv - Cell Biology 2022Quote: ... The PCR products were subcloned into the pcDNA5 FRT/TO HA FLAG Ms Jaw1 (digested with AfeI/BamHI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan) to swap the luminal region of Jaw1 to that of KASH proteins ...
-
bioRxiv - Cell Biology 2022Quote: ... and the DNA fragments were ligated into the pcDNA5 FRT/TO HA FLAG vector (digested with same enzymes) using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan), resulting in pcDNA5 FRT/TO HA FLAG Ms Jaw1 PA and pcDNA5 FRT/TO HA FLAG Ms Jaw1 NIDR PA ...
-
bioRxiv - Biochemistry 2023Quote: ... MBP-Hua2(K221WY -AWY)NT-FLAG and MBP-Hua2(G183KV-AAV)NT-FLAG were constructed by introducing point mutations using In-Fusion HD Cloning (Takara Bio USA, Inc) with the primers listed in Table 1 ...
-
bioRxiv - Microbiology 2023Quote: ... or the BamHI/MluI site of pWPI-ACE2-zeo (for ACE2 expression plasmids)43 with 3×FLAG-tag at the C-terminus using In-Fusion® HD Cloning Kit (Takara, Cat# Z9650N). Nucleotide sequences were determined by DNA sequencing services (Eurofins) ...
-
bioRxiv - Immunology 2021Quote: Flag-RNH1 was further sub-cloned into retroviral vector pMSCVpuro (Clontech). Retroviral vector pMSCVpuro-Flag–RNH1 was co-transfected with the helper plasmids VSV-G and Hit60 into HEK293T cells using PEI transfection reagent ...
-
bioRxiv - Molecular Biology 2023Quote: ... C-FLAG-D20 was subcloned into the pLVX-TetOne-Puro (Clontech) and in-house attb vectors ...
-
Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: Flag-RNH1 was sub-cloned into the retroviral vector pMSCVpuro (Clontech) as described previously 21 ...
-
bioRxiv - Genetics 2020Quote: Myomixer-FLAG was packaged in the pLVX-TetON (Takara Bio; Mountain View, CA) plasmid and have been described in previous literature64 ...
-
bioRxiv - Cell Biology 2019Quote: FLAG-tag-hFAIM-S expression vector was constructed using pCMV-(DYKDDDDK)-C (Takara) (cloned into EcoRⅠ and KpnⅠ sites) ...
-
bioRxiv - Biochemistry 2021Quote: Flag-GFP-tagged UCH37 (WT, C88A, C88S or EWI) were cloned into pQCXIP (Clontech). Retroviruses were produced by co-transfection of pCI-VSVG (a gift from Garry Nolan ...
-
bioRxiv - Cell Biology 2021Quote: ... C-terminally triple FLAG tagged MBP was inserted into pKK plasmids using InFusion cloning (Takara). To introduce C-terminally triple FLAG tagged UPF1 ...
-
bioRxiv - Cell Biology 2020Quote: ... Importin α with an N-terminal FLAG tag was cloned into pLVX-TetOne-Puro (Clontech) to generate stable cell lines ...
-
bioRxiv - Microbiology 2022Quote: ... the FLAG-APEX2-GARG-1060 insert was cloned into the retroviral vector plasmid pLNCX2 (Takara Bio). Cloning details are available upon request ...
-
bioRxiv - Molecular Biology 2020Quote: ... and ligated into the Nhe1 site of the pBS32-Flag-MS2CP parent plasmid using InFusion cloning (Clontech). The PTBP1 Y247Q ...
-
bioRxiv - Genetics 2020Quote: ... in frame with Myc and Flag tag by In-Fusion HD EcoDry Cloning Plus System (Takara Bio). In order to insert a poly histidine tag for protein expression and purification ...
-
bioRxiv - Neuroscience 2022Quote: ... with either 7.5 μg of pcDNA3.1 and pcDNA3.1-p27-Flag (p27 full-length construct) or 7.5 μg of empty pMSCVpuro (Clontech, 634401) and pSUPER.retro mSox2.3 (shRNASox2 target sequence ...
-
bioRxiv - Immunology 2020Quote: ... The IFIT1 amplicon was inserted into the NotI-PmeI digested pEF-Tak-Flag vector by InFusion cloning (Clontech). The following 5’UTR constructs were purchased from IDT as gBlocks ISG15 (RefSeq ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were transfected with eukaryotic expression plasmid harbouring FLAG or MYC-tagged FBXO22 using Xfect (TAKARA Bio) transfection reagent according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... N-terminal Flag-tagged P38α containing 3C protease site was amplified by PCR using CloneAmp HiFi PCR Premix (Takara) and ligated in to pcDNA3 using the aforementioned restrictions sites ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA encoding FLAG-Rhino was cloned into pPB-2× Ty1-Tjen-EGFP-P2A-BlastR51 using In-Fusion cloning (TAKARA), bearing pPB-FLAG-Rhino-Tjen-EGFP-P2A-BlastR ...
-
bioRxiv - Cell Biology 2021Quote: ... The cyclin D1-GFP plasmids were constructed by combining the PCR fragment of cyclin D1 from the cyclin D1-Flag plasmid and GFP from pEGFP-N1 (BD Biosciences Clontech) into XPack CMV constructs (System Biosciences) ...
-
bioRxiv - Cell Biology 2020Quote: ... HeLa cells were transfected with control (scramble) or NUP153-specific shRNA vectors along with FLAG-hNUP153 expression vector using Xfect transfection reagent (Clontech) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: Flp-In™ T-REx™ 293 cell lines stably transfected with Flag-BirA*-TopBP1 WT or W1145R grown to 75% confluence were incubated with 1µg/ml of doxycycline (Clontech, 631311) for 16h and with 50µM biotin for 3 or 16 hours ...
-
bioRxiv - Immunology 2022Quote: ... The following plasmids were generated by insertion of PCR-amplified fragments into the NotI-to-PmeI digested pEF-TAK-Flag using InFusion cloning (Clontech): pEF-TAK-Flag-UFL1 (GenBank ...
-
bioRxiv - Immunology 2019Quote: ... pEF-TAK-HA-RAB1B was generated by PCR amplification of RAB1B from pEF-TAK-Flag-RAB1B and insertion into the EcoRI-XbaI digested pEF-vector using InFusion cloning (Clontech). The pcDNA-Myc-TRAF3 plasmid was generated by cloning the PCR-amplified TRAF3 (plEGFP-N1-TRAF3 ...
-
bioRxiv - Biophysics 2021Quote: ... A cDNA coding for 128QHTT with C-terminal fusion to a FLAG-His affinity tag was cloned into the vector pTRE-tight-BI-AcGFP1 (Clontech) for expression of 128QHTT upon induction with Dox ...
-
bioRxiv - Cancer Biology 2021Quote: ... whereas a TOPBP1 fragment corresponding to residues 2-1523 was amplified from pCDNA5-FRT/TO-LacR-FLAG-TopBP1 and cloned into pGBKT7 (Clontech/Takara) to create a fusion with the GAL4 DNA binding domain using the NdeI and XmaI sites ...
-
bioRxiv - Plant Biology 2019Quote: The full-length cDNA of NaERF2-like with two HA flags was cloned into pCAMBIA1301 vector after 35S promoter via in-fusion technique (Clontech). N ...