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Citations for Takara Bio :
1 - 50 of 4649 citations for Alkaline Phosphatase Colorimetric Activity Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The calf intestinal alkaline phosphatase (TaKaRa) was used as a standard for the reaction.
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bioRxiv - Molecular Biology 2023Quote: ... 1 μL of alkaline phosphatase (Takara, Okasa, Japan) was added ...
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bioRxiv - Molecular Biology 2019Quote: ... an alkaline phosphatase (AP) tag (pSEAP; Clontech, CA, USA) was linked to the N-terminus of PAR1 cDNA (F2R ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 0.5 U/ml Bacterial Alkaline phosphatase (Takara 2120A) at 37°C for 1 h in 10 µl of a reaction mixture containing 20 mM HEPES–KOH pH 7.5(Nacalai 15639-84) ...
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bioRxiv - Cell Biology 2023Quote: ... secreted embryonic alkaline phosphatase (SEAP) activity and TGF-β concentration were determined using the Great EscAPe® SEAP (631738, TaKaRa Bio, Kusatsu, Japan) chemiluminescence kit to quantify SEAP activity (excitation wavelength 360 nm ...
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bioRxiv - Plant Biology 2019Quote: ... and dephosphorylated with Calf Intestinal Alkaline Phosphatase (Takara, cat#2250A). CRISPR targets were synthesized in oligonucleotide form and annealed to produce a double stranded fragment which was subsequently ligated to the BsaI treated vectors (Takara ...
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bioRxiv - Biochemistry 2024Quote: ... unreacted ligation oligo was dephosphorylated using alkaline phosphatase (Calf intestine) (Takara) and formamide dye [10 mM EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... After de-phosphorylation of the linear vector using Shrimp Alkaline Phosphatase (Takara, 2660A), the cDNA and the linear vector were mixed and ligated.
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bioRxiv - Cell Biology 2023Quote: ... DUSP13 deficient in phosphatase activity (=Dusp13_Mut) was generated using the PrimeSTAR® Mutagenesis Basal Kit (Takara Bio). The Dusp family contains the amino acid cysteine ...
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bioRxiv - Cell Biology 2022Quote: ... as described previously.15 Alkaline phosphatase (ALP) staining was also performed for pluripotency characterization using TRACP & ALP double-stain Kit (Takara) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... qPCR-confirmed wsp-positive products were cleaned using a mixture of alkaline phosphatase (TaKaRa) and exonuclease I (TaKaRa) ...
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bioRxiv - Plant Biology 2019Quote: ... 30 µg of microsomal proteins were then treated with 30 units of calf intestinal alkaline phosphatase (TaKaRa) at 37°C for 2 h ...
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bioRxiv - Cell Biology 2019Quote: RNA from stress granules or cytosolic mRNAs from heated HeLa cells were resuspended in 10 μl of MS water and digested with 0.05 units of nuclease P1 (Wako) and 0.05 units of bacterial alkaline phosphatase (BAP C75, Takara) in P1 buffer (20 mM ammonium acetate pH 5.3 ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was tested using gel electrophoresis (1.5% agarose) and products that successfully amplified were cleaned with Exonuclease I and Shrimp Alkaline Phosphatase (Takara, Japan). The cleaned PCR products were cycle-sequenced using a BigDye™ Terminator v3.1 Cycle Sequencing Kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA from each candidate cell line was digested to single nucleoside using nuclease P1 (Fujifilm Wako Pure Chemical Corporation) and bacterial alkaline phosphatase (TaKaRa), followed by mass spectrometry analysis ...
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bioRxiv - Genomics 2021Quote: ... 10 ng of cfDNA was dephosphorylated in a 10-μL reaction containing 2.5 μL of 10× TACS buffer and 1 μL of shrimp alkaline phosphatase (Takara Bio Inc.) at 37 °C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... Lactate dehydrogenase activity was quantified using the LDH Cytotoxicity Detection Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Media lactate dehydrogenase (LDH) was assayed by enzymatic colorimetric assay (LDH Cytotoxicity Detection Kit, Takara Bio, cat# MK401).
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bioRxiv - Microbiology 2022Quote: NK cell activity was quantified using the LDH Cytotoxicity Detection Kit (MK401, TaKaRa, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... then 100 µL of this supernatant was used to evaluate LDH release into the culture medium as a measure of host cells lysis using a colorimetric cytotoxicity detection kit (Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... the Lactate dehydrogenase (LDH) activity in the supernatant was measured with LDH Cytotoxicity Detection Kit (Takara) according to the manufacturer’s instruction.
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bioRxiv - Biochemistry 2022Quote: ALP activities were measured using a Great EscAPe™ SEAP kit (Takara Bio Inc., Shiga, Japan) in lysates of PIGT+ and PIGT− cells ...
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bioRxiv - Cell Biology 2021Quote: Cell lysis was assessed by measuring Lactate Dehydrogenase (LDH) activity in cell supernatants using the LDH cytotoxicity detection kit (TaKaRa). To obtain the positive control ...
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bioRxiv - Synthetic Biology 2023Quote: ... SEAP activity in supernatant (25 μL) was measured using the Great EscAPe SEAP Chemiluminescence Assay kit 2.0 (Clontech, Fremonet, CA). The GLuc activity in supernatant (20 μL ...
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bioRxiv - Microbiology 2023Quote: ... SEAP activity was quantified using the Great EscAPe SEAP assay (Clontech). Fluorescence was read on a Filtermax F5 plate reader (Molecular Devices).
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bioRxiv - Immunology 2022Quote: Lytic cell death was determined by measuring LDH release from cell-free supernatants using a colorimetric assay (Promega or Takara) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Both pFCDUET-YopH phosphatase and pGKJE8-GroEl/GroEs chaperones plasmids (Takara chaperone plasmid set cat. # 3340) were used for co-expression experiments ...
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bioRxiv - Plant Biology 2020Quote: ... Plasmids for the PARN activity assay were constructed by inserting the coding sequence of RRD1 or human PARN (hPARN) into the pHAT vector (Clontech). The hPARN sequence was derived from the GNP Human cDNA clone IRAK071M01 (RIKEN BioResource Research Center) ...
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bioRxiv - Microbiology 2022Quote: ... A second construct for total gene deletion was generated by cloning the 5’ (843 bp) and 3’ (292 bp) homology regions amplified by PCR using a Taq DNA polymerase with proofreading activity (Takara) and primers P9 + P12 and P13 + P14 into the pL0001 vector (BEI Resources ...
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bioRxiv - Plant Biology 2022Quote: ... β-galactosidase activity in liquid cultures was assayed using CPRG as substrate as described in the Yeast Protocols Handbook (Takara).
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bioRxiv - Molecular Biology 2020Quote: ... The relative luciferase activity was determined by normalizing the firefly luciferase activity by the respective total EGFP protein amount quantified by Western blot using a GFP antibody (Clontech, 632460) in Image Studio ver 5.2 (LI-COR).
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bioRxiv - Cell Biology 2023Quote: ... The β-galactosidase activity was determined by ONPG (o-nitrophenyl β-D-galactopyranoside) liquid assay (quantitative) as mentioned elsewhere (Clontech Laboratories, 2008). β-galactosidase activity from each sample was calculated from at least three biological replicates and the concentration of cells (O.D.600 ...
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bioRxiv - Developmental Biology 2021Quote: ... Extent of SRY-dependent expression of β-galactosidase was evaluated by quantitative assay of enzyme activity in liquid culture using ortho-nitrophenyl-β-galactoside (ONPG) as described by the vendor (Clontech Laboratories, Inc). Mammalian Cell Culture—Rodent preSertoli-cell line ...
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bioRxiv - Synthetic Biology 2019Quote: ... Muc14a_5 and Muc14a_6 and Cas9 were tested for in vitro activity using the Guide-it sgRNA In Vitro Transcription and Screening System (Takara Bio USA, Inc.). Each kmer was transcribed and purified according to the In Vitro Transcription of sgRNA protocol ...
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bioRxiv - Immunology 2022Quote: ... Seq Kit (Takara), as per the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was retro-transcribed by Takara kit (Takara Bio), GAPDH was used as internal reference ...
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bioRxiv - Developmental Biology 2021Quote: ... In-fusion HD kit and In-Fusion Snap Assembly kit (TaKaRa). pRSETa_mEos4b was a gift from Loren Looger (Addgene plasmid #51073 ...
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bioRxiv - Cancer Biology 2023Quote: ... LipofectamineTM 2000 kit and reverse transcription kit were purchased from TaKaRa Company ...
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bioRxiv - Immunology 2024Quote: ... The RNeasy kit and the cDNA Synthesis Kit (Takara, Cat#: 9767, RR047A) was used to extract total RNA and prepare cDNA following the manufacturer’s instructions ...
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Diversified expression of gamma-protocadherins regulates synaptic specificity in the mouse neocortexbioRxiv - Neuroscience 2021Quote: LATaq Kit (RR002B, TaKaRa) was used in nested PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... 3’RACE kit (Takara) was used to convert RNAs of the prostate tumors into cDNAs by a reverse transcriptase and oligo-dT adapter primer ...
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Effects of Sanhuang plaster on expression of MyD88, TRAF6, MIP-1β and IL-23 in rats infected by MRSAbioRxiv - Molecular Biology 2022Quote: ... Real-time fluorescence quantitative PCR kit and reverse transcription kit(TaKaRa Co., Ltd.); MyD88 Anti-body TRAF6 Anti-body MIP-1β Anti-body and IL-23 Anti-body (GeneTexCo. ...
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bioRxiv - Biophysics 2020Quote: ... using a cDNA synthesis kit (PrimeScript 1st strand cDNA Synthesis Kit, Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Adeno-X Maxi Purification Kit and Adeno-X Rapid Titer Kit from Clontech were used for purification and titration ...
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bioRxiv - Cancer Biology 2023Quote: ... Adeno-X Maxi Purification Kit and Adeno-X Rapid Titer Kit from Clontech were used ...
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bioRxiv - Systems Biology 2022Quote: ... The SMART-Seq Library Prep Kit and Unique Dual Index Kit (TaKaRa, cat# R400745) were used to generate barcoded template library for sequencing ...
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bioRxiv - Cell Biology 2019Quote: ... Calcium phosphate transfection kit (Takara) was used for transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... 3.0 kit (Takara Bio, Inc.) and cloned into the EcoRI/XhoI sites of the pET32a (+ ...
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bioRxiv - Cell Biology 2021Quote: ... SYBR Green PCR kit (Takara) was applied to conduct RT-qPCR ...