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Citations for Takara Bio :
1 - 50 of 813 citations for 6 OXABICYCLO 3.2.1 OCT 3 EN 7 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... was obtained from Takara (Saint-Germain-en-Laye, France). IR dye conjugated secondary antibodies were used at a dilution of 1:20,000 and purchased from LI-COR Biosciences (Cambridge ...
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bioRxiv - Cell Biology 2020Quote: ... was obtained from Takara (Saint-Germain-en-Laye, France). Rat HA antibody (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... AP20187 (B/B) (Clontech, Saint-Germain-en-Laye France) was added 4 h post-transfection overnight.
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bioRxiv - Genomics 2021Quote: ... Stellar competent cells (Clontech, TaKaRa, Saint-Germain-en-Laye, France) were transformed with the ligated vector and plated ...
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bioRxiv - Genomics 2021Quote: ... Stellar competent cells (Clontech, TaKaRa, Saint-Germain-en-Laye, France) were transformed with the ligated vector and plated ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was introduced to measure the relative expression of RDase genes by using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (Takara Bio, Saint-Germain-en-Laye, Germany). Primers targeting RDase genes were designed using the NCBI primer design tool with the setting parameters ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... RetroNectin (12 µg/mL, Takara Bio, Saint-Germain-en-Laye, France) was coated and the previously mentioned cytokines were added in combination with polybrene (8 µg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... or PrimeSTAR GXL DNA Polymerase (Takara Bio, Saint-Germain-en-Laye, France) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... The Lenti-XTM HTX Packaging System (Clontech, Saint-Germain-en-Laye, France) was used with the appropriate plasmid (generated by DC Bioscience ...
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bioRxiv - Synthetic Biology 2022Quote: ... or PrimeSTAR GXL DNA Polymerase (Takara Bio, Saint-Germain-en-Laye, France) according to the manufacturers’ recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP (JL-8, 1:3000, Clontech, Saint-Germain-en-Laye, France) and anti-Actin (AC74 ...
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bioRxiv - Immunology 2020Quote: Lenti-X™ 293T cells (Takara Bio, Clontech, Saint Germain en Laye, France) were grown using complete Dulbecco’s Modified Eagle Medium F-12 (DMEM F-12 ...
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bioRxiv - Immunology 2021Quote: ... and PrimeScript™ RT Reagent Kit (RR037B, Takara Bio, Saint-Germaine-en-Laye, France), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with TALON resin (Takara Biotech, Saint-Germain-en-Laye, France) for 90 min in a ratio of 1 ml resin per 10 g of cells ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were seeded in one well of a 6 well plate in supplemented DEF-CS (Takara). After a recovery period of 5 days ...
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bioRxiv - Biophysics 2019Quote: ... The supernatant was incubated with 2 mL TALON resin (Takara, Saint-Germain-en-Laye, France) in a tube using a rotation shaker for 30-60 minutes ...
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bioRxiv - Microbiology 2021Quote: ... and 0.7 U of Terra PCR Direct Polymerase (ClonTech Europe, Saint-Germain-en-Laye, France).
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bioRxiv - Cell Biology 2023Quote: ... All other constructs were generated by In-Fusion Cloning (Takara, Saint-Germain-en-Laye, France) using the manufacture’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR was performed with Advantage-HD DNA polymerase (Takara Bio, Saint-Germain-en-Laye, France) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: The amplified library was then recombined with pCMV FAS wt minigene exon 5-6-7 (Förch et al., 2000) using the In-Fusion HD Cloning kit (639649, Clontech) in a 1:8 vector:insert optimized ratio and transformed into Stellar competent cells (636766 ...
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bioRxiv - Genomics 2020Quote: ... for subsequently cloned using the InFusion HD Cloning Kit protocol (Takara Bio, Saint-Germain-en-Laye, France). All plasmids were confirmed by Sanger sequencing ...
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bioRxiv - Genetics 2021Quote: ... and cloned into the equally digested bait DNA vector pAbAi (Takara Clontech, Saint-Germain-en-Laye, France). The yeast strain S ...
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bioRxiv - Immunology 2020Quote: ... filtered (0.45□μm) and concentrated using Lenti-XTM Concentrator (Takara Bio, Clontech, Saint Germain en Laye, France).
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bioRxiv - Microbiology 2024Quote: ... The reverse transcription was performed using the PrimeScript RT reagent kit (Takara, Saint-Germain-en-Laye, France) with a primer oligo-d(T)-AP (5’ GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTv 3’) ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies were used: anti-GFP (JL-8; 1:20, Clontech, Saint-Germain-en-Laye, France), anti-PHB1 (EP2803Y ...
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bioRxiv - Cell Biology 2019Quote: ... Point mutants were generated using the In-Fusion HD cloning kit (TakaraBio/Clontech, Saint-Germain-en-Laye, France). DNA sequencing was carried out by LGC Genomics (Berlin ...
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bioRxiv - Cell Biology 2020Quote: ... we took advantage of the In-Fusion® HD Cloning system (Takara Bio Europe, Saint-Germain-en-Laye, FR) to generate a PiggyBac™ transposon system (PB ...
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bioRxiv - Developmental Biology 2021Quote: ... was incubated with antibodies at 4°C for one hour: 3 µl (0.5 µg/µl) c-Myc monoclonal antibody (Cat. No. 631206, TaKaRa), or 1 µl (1.1 µg/µl ...
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bioRxiv - Immunology 2020Quote: ... Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio, Clontech, Saint Germain en Laye, France) and resuspended in phenol red-free medium ...
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bioRxiv - Microbiology 2021Quote: ... 0.4 µ mol of each primer and 0.7 U of Terra PCR Direct Polymerase (ClonTech Europe, Saint-Germain-en-Laye, France).
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bioRxiv - Cell Biology 2020Quote: ... Point mutants were generated by site-directed mutagenesis using the In-Fusion HD cloning kit (TakaraBio/Clontech, Saint-Germain-en-Laye, France). Sequence correctness was verified by sequencing (LGC Genomics ...
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bioRxiv - Plant Biology 2022Quote: Yeast one-hybrid assays were performed using the Matchmaker Yeast One-Hybrid System (Clontech). The DNA fragment 1,000 bp upstream of the start codon ATG of DDRM2 was cloned into pHis2.1 vector and SOG1 was cloned into the pGADT7 vector ...
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bioRxiv - Plant Biology 2019Quote: ... strategy and internal primers designed from the genomic contigs (Table S1) following the kit supplier’s recommendations (Takara Bio Europe/Clontech, Saint Germain-en-Laye, France). The 3’ ends were amplified using forward internal and polyA-anchored LD primers (Table S1 ...
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bioRxiv - Cancer Biology 2022Quote: ... pooled and filtered through a 0.45 μm syringe filter and then concentrated using the Lenti-X Concentrator solution (Clontech/Takara, Saint-Germain-en-Laye, France). Intestinal WT organoids were expanded 3 days prior to infection in normal growth medium supplemented with 1 μg/ml R-Spondin ...
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bioRxiv - Immunology 2020Quote: TZM-bl (kindly provided by Dr. Quentin Sattentau, University of Oxford) and Lenti-XTM 293T cells (Takara Bio, Clontech, Saint Germain en Laye, France) were cultured in either complete DMEM or complete DMEM F-12 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... the U2OS 2-6-3 cells expressing degron-tagged LacI fusion proteins were incubated with 300 nM Shield1 ligand (Takara Bio) and 1 µg/mL doxycycline for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... 0.5 µg of the isolated RNA was reverse transcribed to cDNA using an oligo dT primer and 12.5 units of reverse transcriptase mix (PrimeScript RT Reagent Kit, TaKaRa Bio, Saint-Germain-en-Laye, France). cDNA corresponding to 10 ng total RNA served as the template in a real-time PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... pooled and filtered through a 0.45 μm syringe filter and then concentrated using the Lenti-X Concentrator solution (Clontech/Takara, Saint-Germain-en-Laye, France). Intestinal WT organoids were expanded 3 days prior to infection in normal growth medium supplemented with 1 μg/ml R-Spondin ...
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bioRxiv - Bioengineering 2021Quote: ... 7 μL RNase-free water (Takara, Japan), and 10 μL iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: Yeast one-hybrid assays were performed using the Matchmaker Gold Yeast One-Hybrid System Kit (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: A codon-optimized synthetic gene corresponding to the full length nucleocapsid protein (Thermo GeneArt, Regensburg, Germany) was cloned into a modified pET28a vector using the In-Fusion cloning kit (Takara Bio Saint-Germain-en-Laye, France). The resulting plasmid encoded for an N-terminal His6 tag followed by thrombin and tobacco etch virus cleavage sequences upstream of the full-length nucleocapsid protein sequence ...
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bioRxiv - Biochemistry 2024Quote: ... a two-round PCR strategy was required to amplify the ORF using a high-fidelity PrimeStar ® DNA polymerase (Takara Europe, Saint-Germain-en-Laye, France). The first round of the nested PCR was carried out using the above cDNA as the template with primers designed in the 5’- and 3’-untranslated regions (UTR ...
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bioRxiv - Plant Biology 2021Quote: ... yeast one-hybrid assays were performed as the manual of Matchmaker Gold Yeast One-Hybrid System (Clontech). The coding sequence of BnaA03.WRKY28 was cloned into pGADT7 vector ...
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bioRxiv - Cell Biology 2021Quote: ... One-step qPCR was performed using One Step PrimeScript™ III RT-qPCR Mix (Takara, Kusatsu, Japan) with primers as follows ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were assembled into pEMC vector one-by-one using in-fusion HD cloning kit (Takara), generating pEMC-eGFP-MBaMV ...
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bioRxiv - Molecular Biology 2023Quote: ... These bulk kidney tissues were process from an OCT block using the SMARTer Stranded Total RNA-Seq Kit v2 (Takara). Sequencing was performed in a 2×75 bp paired-end configuration using a NovaSeq platform (Illumina).
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bioRxiv - Molecular Biology 2022Quote: ... pLVX-Tet-One-Puro vector (Clontech, #631847), encoding for an engineered Tet activator and the corresponding inducible promoter ...
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bioRxiv - Plant Biology 2019Quote: The Matchmaker yeast-one-hybrid system (Clontech) was used to test the binding of NaERF2-like and the NaEAS12 promoter in vitro according to the user manual ...
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bioRxiv - Microbiology 2023Quote: ... expression plasmid pLVX Tet-One Puro (Clontech) was modified to have a MCS at the 3’end of Tet responsive promoter TRE3GS using EcoR1/BamH1 cut sites ...
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bioRxiv - Cell Biology 2020Quote: ... we designed primers with complementary overhangs (Supplementary Table S3) and used them to generate a “NEON insert” by PCR amplification (high-fidelity DNA polymerase, CloneAmp™, Takara Bio Europe, Saint-Germain-en-Laye, FR) of the mNeonGreen sequence (Allele Biotechnology and Pharmaceuticals Inc. ...