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Citations for Takara Bio :
401 - 450 of 5042 citations for Pyruvate Dehydrogenase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... or In-Fusion HD Cloning Kit (TaKaRa). The GFP-fused full-length NCAN-expressing plasmid was constructed by inserting the fragment encoding GFP amplified from the pCAG-GFP vector into the central region of NCAN (corresponding to Val641 to Leu644) ...
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bioRxiv - Microbiology 2022Quote: ... Mink ACE2 full length gene was amplified using long range PCR (LRPCR) amplification assay in two overlapping fragments using high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Bio USA) as previously described (34,35) ...
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bioRxiv - Plant Biology 2021Quote: ... The yeast strain AH109 was used for the yeast two-hybrid assay according to MatchmakerTM Two-Hybrid System 3 (Clontech, Palo Alto) and grown on YPD (full medium ...
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bioRxiv - Plant Biology 2024Quote: The Y1H assays were conducted according to the manufacturer’s instructions (Matchmaker Gold Y1H Library Screening System; Clontech Laboratories, Mountain View, CA, USA). The pGADT7 empty vector with the pHis2 promoter and pGADT7-ZmHsf20 with the pHis2 empty vector were used as negative controls ...
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bioRxiv - Cell Biology 2023Quote: ... The β-galactosidase activity was determined by ONPG (o-nitrophenyl β-D-galactopyranoside) liquid assay (quantitative) as mentioned elsewhere (Clontech Laboratories, 2008). β-galactosidase activity from each sample was calculated from at least three biological replicates and the concentration of cells (O.D.600 ...
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bioRxiv - Bioengineering 2022Quote: HEK293T cells were harvested for RNA extraction using RNeasy Plus Mini Kit (Qiagene) or NucleoSpin RNA Plus Kit (Takara). RNA concentration was measured by nanodrop and 500ng to 1000ng total RNAs were used for reverse transcription in 10 ul reaction volume by High-Capacity RNA-to-cDNA kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription (RT) was then initiated with the cDNA synthesis kit (PrimeScript™ 1st strand cDNA Synthesis Kit; Takara) using the random hexamers option ...
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bioRxiv - Microbiology 2021Quote: ... RNA was converted to cDNA with the PrimeScript™ RT reagent Kit Prime Script TMRT reagent Kit (Takara, Japan), and the cDNA was analyzed by qRT-PCR using TB Green® Premix Ex TaqTM II (Takara ...
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bioRxiv - Microbiology 2020Quote: 5’-RACE analysis was performed using SMARTer RACE 5’/3’ kit and In-Fusion HD Cloning kit according to the manufacturer’s instructions with slight modifications (Clontech). 5’-RACE ready cDNA was synthesized from purified mRNA (TG ...
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bioRxiv - Immunology 2021Quote: ... Cells were harvested after 3 d and AAV6 was purified with a filtration-based kit (Takara AAVpro Purification Kit) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The extracted probes were radioactively labeled with 2.5 μl [alpha-32P] dCTP (Amersham) according to the instructions of the kit (LaddermanTM Labeling Kit, Takara). The probe was mixed with 100 μl TE buffer (10mM ...
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bioRxiv - Molecular Biology 2022Quote: ... library preparation was performed using the SMARTER Stranded Total RNA Seq kit v2-Pico Input Mammalian kit (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... chimeric fluorescence detection kit and TB Green Premix Ex Taq™ (Tli RNaseH Plus) kit were obtained from Takara Biomedical Technology (Beijng ...
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bioRxiv - Zoology 2024Quote: ... and reverse transcribed using the PrimeScriptTM RT reagent Kit with gDNA Eraser (Perfect Real Time) kit (Takara, Dalian, China). The RT-qPCR reactions were carried out on the PikoReal 96 Real-Time PCR System (Thermo ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was isolated (HiPure Plant RNA MIni Kit, Magen) and reverse transcribed (The PrimeScript® RT reagent Kit, Takara). Two pairs of primers (5′-GGCAAGAATCATCACGACCAG-3′ and 5′-GTATGCCATGAGGTCGTCCAC-3′ ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from wing discs of individual silkworms at the wandering stage using the MicroElute Total RNA Kit (OMEGA) and reverse transcription was performed using the PrimeScript RT reagent Kit (Takara). qRT-PCR experiments were performed using Hieff SYBR Green Master Mix (YEASEN) ...
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bioRxiv - Microbiology 2021Quote: ... Remaining DNA was removed using the TURBO DNA-free kit (Thermo-Scientific) and cDNA generated using the PrimeScript cDNA synthesis kit (Takara). qRT-PCR reactions were set up according to the manufacturer’s instructions (Alkali Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were prepared using the SMART-Seq v3 Ultra Low RNA Input Kit for Sequencing and the Low Library Prep Kit v1 from Clontech. For the an3 RNAseq experiment ...
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bioRxiv - Molecular Biology 2020Quote: ... the cDNAs were produced using BioRad Reverse Transcription Kit (iScriptTM Reverse Transcription Supermix) or the PrimeScript RT reagent kit (RR037A, TaKaRa). cDNAs from HEK and THP89 cells were quantified by quantitative PCR using Bio Rad’s SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Immunology 2021Quote: ... Following bulk BCR and TCR are prepared using SMARTer Mouse BCR IgG H/K/L Profiling Kit and SMARTer Mouse TCR a/b profiling kit separately (Takara). Based on the extracted mRNA amount of each sample ...
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bioRxiv - Neuroscience 2022Quote: ... RNA with RIN > 7 was prepared for sequencing using the RNAseq Ovation V2 kit (Nugen) or Smart-seq 4 low-abundance RNA kit (Takara) and sequenced on the Illumina HiSeq 4000 or Novaseq 2 platform in collaboration with Oxford Genomics Centre.
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bioRxiv - Microbiology 2020Quote: ... Total viral DNA was extracted using Qiagen viral DNA extraction kit (QIAamp DNA Mini Kit, Hilden, Germany) and DNA polymerase Tag (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and indexing were performed as previously described using the Qiagen AllPrep DNA/RNA Micro Kit and the SMARTer Stranded Total RNA-Seq Kit v2 (Takara) (46 ...
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bioRxiv - Molecular Biology 2019Quote: ... Directional libraries were prepared using the Smarter Stranded Total RNA-Seq kit-Pico Input Mammalian kit following the manufacturer’s instructions (Clontech, 635005). The quality of all libraries was verified with the DNA-1000 kit (Agilent ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA synthesis and library preparation was performed using the SMART-Seq v4 Ultra Low Input RNA Kit and Low Input Library Prep Kit v2 (Clontech), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA synthesis and library preparation was performed using the SMART-Seq v4 Ultra Low Input RNA Kit and Low Input Library Prep Kit v2 (Clontech), respectively ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the manufacturer specifically states they should not occur in RNA-Seq reads if used in combination with the Nextera XT DNA library preparation kit (Appendix C in SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing User Manual, Takara Bio Inc ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA libraries were prepared with the Trio RNA-seq + UDI Library Preparation Kit (NuGEN) or the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Clontech) and sequenced on the MiSeq ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing libraries were prepared using “SMARTer Stranded Total RNA-seq Kit v3 – Pico Input Mammalian” kit (Takara Bio, Cat. # 634487) and the “SMARTer RNA Unique Dual Index Kit - 24U” (Takara Bio ...
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bioRxiv - Cancer Biology 2024Quote: ... The full-length cDNA of the human ZNRF3 was acquired from the human CRC DLD-1 cell line by RNA purification using NucleoSpin RNA II kit (Macherey Nagel) and reverse transcription RT-PCR using specific primers and Primescript RT reagent kit (TaKaRa). Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using a NucleoSpin RNA kit (Macherey Nagel) and reverse transcribed with the Primescript RT reagent kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... A total of 3.5 μl ribo-depleted material was used for SMARTer (SMARTer PCR cDNA Synthesis Kit, catalog num. 634926 and Advantage 2 PCR kit, catalog num. 639207, Clontech-Takara) protocol ...
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bioRxiv - Microbiology 2023Quote: ... Remaining DNA was removed using the TURBO DNA-free kit (Thermo-Scientific) and cDNA generated using the PrimeScript cDNA synthesis kit (Takara). qRT-PCR reactions were set up according to the manufacturer’s instructions (Alkali Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Isolated RNA was dissolved in 10 μl of DEPC-treated water and reverse-transcribed using a reverse transcription reagent kit (PrimeScript RT Reagent Kit with gDNA Eraser, Takara) and a thermal cycler (Mastercycler ...
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bioRxiv - Neuroscience 2024Quote: ... were purchased from Thermo Fisher Scientific. cDNA synthesis kit (cat no. 6110B) and real-time PCR kit (cat no. PN 4367659) were purchased from TAKARA. ECL reagent (cat no ...
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bioRxiv - Microbiology 2020Quote: For Bioluminescence Resonance Energy Transfer (BRET) assay we used the pEYFP-C1/N1 plasmids encompassing EYFP tag in the N- or C-terminal positions (Clontech, Mountain View, CA). To obtain pNluc-C1/N1 plasmids ...
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bioRxiv - Developmental Biology 2021Quote: ... Extent of SRY-dependent expression of β-galactosidase was evaluated by quantitative assay of enzyme activity in liquid culture using ortho-nitrophenyl-β-galactoside (ONPG) as described by the vendor (Clontech Laboratories, Inc). Mammalian Cell Culture—Rodent preSertoli-cell line ...
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bioRxiv - Neuroscience 2020Quote: ... The reaction was performed in a final volume of 12 μL using the Premix Ex Taq Probe based qPCR assay (Takara Biotechnology, ref. RR390A). All reactions were run in duplicate ...
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bioRxiv - Microbiology 2020Quote: ... SYBRgreen real-time quantitative assays were made by using the SYBR® Premix Ex Taq™ II (Tli RNaseH Plus; Takara Bion Inc.) and an Applied Biosystems® Step One PCR System ...
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bioRxiv - Physiology 2024Quote: Flies treated with the same procedure as the lifespan assay were collected after 7-day EF/Sham exposure and homogenized in RNAiso reagent (Takara Bio, Shiga, Japan). Ten to twenty flies were homogenized in one tube ...
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bioRxiv - Neuroscience 2023Quote: ... The qPCR reaction was performed on 96-well plates in a final volume of 12 μL using the Premix Ex Taq Probe based qPCR assay (Takara Biotechnology, ref. RR390A). All reactions were run in duplicate on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... selections and LacZ reporter assays were made according to the Matchmaker Gold Yeast Two-Hybrid System user manual (Clontech; Mountain View, Ca, USA).
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bioRxiv - Cancer Biology 2021Quote: ... a Prime Script RT kit (Takara, Tokyo, Japan) was adopted for the generation of complementary DNA ...
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bioRxiv - Systems Biology 2020Quote: Human mitochondrial to nuclear DNA ratio kit (Takara) was used to assess mitochondrial DNA content ...
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bioRxiv - Neuroscience 2020Quote: We used the In-Fusion cloning kit (Takara) to amplify the cDNA of the long isoform of UNC-13 (UNC-13L ...
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bioRxiv - Microbiology 2021Quote: ... ligated together using Mighty Mix ligation kit (Takara), cloned into competent DH5α E ...
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bioRxiv - Developmental Biology 2020Quote: ... using the In-fusion HD cloning kit (Clontech) according to supplier’s instructions.
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bioRxiv - Genetics 2022Quote: ... GenTLE (from yeast) high recovery kit (Takara Bio). The genomic DNA concentration was measured using the Quantifluor® ONE dsDNA Dye System (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... and SMARTer DNA Unique Dual Index Kit (TAKARA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: The SMART® cDNA Library Construction Kit (Clontech) was used to reverse transcribe the zebrafish heart RNA ...