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Citations for Takara Bio :
1151 - 1200 of 2499 citations for PCR Strips since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... Mir-X miRNA qRT-PCR TB Green kit (Takara BIO, San Jose, CA, USA) was used for cDNA synthesis and miRNA expression analysis ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR was performed using TB Green Premix Ex Taq II (TaKaRa Bio, Japan) on a StepOne Real-time PCR (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and CEP44 mutants were amplified via PCR and cloned into pEGFP-N1 (Clontech Laboratories) and p3×FLAG-CMV-14 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative RT-PCR was carried out using SYBR Premix ExTaq™ (Takara Biotechnology, Dalian, China). The primers used in this study were listed in Supplementary Table 3.
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bioRxiv - Cell Biology 2020Quote: ... Quantitative RT-PCR was performed by using SYBR Premix Ex Taq (Takara Bio, Dalian, China) and the following primers ...
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bioRxiv - Neuroscience 2021Quote: ... the cpmApple module from jRGECO1a was amplified by PCR and inserted by In-Fusion (Clontech) to the ICL3 region of human DRD1 in the pcDNA5 vector ...
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bioRxiv - Molecular Biology 2021Quote: ... Real-time PCR was performed using TB Green Premix Ex Taq™ II (Takara Bio) on a StepOnePlus™ real-time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time PCR was performed using the SYBR Premix Ex Taq™ (Cat# DRR041A, Takara) on a LightCycler 480 Instrument II Real-Time PCR Detection System (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... DNA from each ChIP sample and the corresponding input sample was purified (PCR Cleanup, Takara) and analyzed further using qPCR ...
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bioRxiv - Genomics 2020Quote: Copy DNAs were generated from polyA(+) RNA fractions using SMARTer PCR cDNA Synthesis Kits (Clontech) according to PacBio Isoform Sequencing (Iso-Seq ...
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bioRxiv - Microbiology 2020Quote: DNA encoding the eGFP sequence was PCR amplified out of the eGFP-N2 plasmid (Clontech) and subcloned via single-cut restriction digest into the corresponding UL25 plasmid harboring the cleavable His6-SUMO tag [(either UL25Δ50 (pED13 ...
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bioRxiv - Developmental Biology 2019Quote: ... Complementary DNA was synthesized with 1μgtotal RNA using a PrimeScript™ RT-PCR Kit (Takara). The real-time PCR with the mixture reagent SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: All PCR for assembling transforming DNA were obtained by proof-reading polymerase (PrimeStar Max, Takara). Genomic DNA from Legionella was isolated using the Wizard Genomic DNA Purification Kit or the Maxwell DNA extraction kit (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Reactions were performed in duplicate using Tli RNaseH plus and universal PCR master mix (Takara). The relative expression was calculated by the 2(−ΔΔCt ...
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bioRxiv - Cancer Biology 2019Quote: ... The real-time quantitative PCR was performed by using SYBR Green Master Mix (Takara, RR420A) in StepOnePlus 96 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... Results were quantified using real-time PCR with SYBR premix Ex Taq II solution (TaKaRa). Chromatin immunoprecipitation (ChIP ...
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bioRxiv - Genomics 2019Quote: ... and on-chip PCR was performed using a SMARTer ICELL8 Thermal Cycler (Takara Bio USA) as follows ...
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bioRxiv - Cell Biology 2019Quote: ... PCR products were inserted into the respective plasmids using the In-Fusion cloning system (Clontech), the sequence- and ligation-independent cloning (SLIC ...
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bioRxiv - Cell Biology 2019Quote: ... followed by PCR-mediated amplification and plasmid insertion with the in-Fusion cloning kit (Clontech). The CLN6ΔL2 construct was obtained by removing the codons for amino acids 155-222 using the Q5 Site-Directed Mutagenesis kit (New England Biolabs).
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bioRxiv - Genetics 2019Quote: ... The lentiviral titers were determined with a Lenti-X™ qRT-PCR Titration Kit (Clontech).
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bioRxiv - Molecular Biology 2019Quote: ... The PCR product was cloned into the corresponding restriction sites of pEGFP-N1 vector (Clontech).
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bioRxiv - Immunology 2021Quote: ... The indicated chimeras of BRD7 and BRD9 were generated by overlapping PCRs or InFusion (Takara). Remaining variants of BRD9 were generated by overlap-extension PCR using Q5 Hot Start High-Fidelity (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was synthesized from 1μg of RNA via the One Step RT-PCR Kit (TaKaRa). Quantitative real time PCR was performed using the iQTM SYBR Green Supermix PCR kit with the iCycler apparatus system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using SYBR® Premix Ex Taq™ (TaKaRa) in a Bio-Rad CFX96™ real-time PCR system using the following thermocycling conditions ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time quantitative RT-PCR was performed with TB Green Premix Ex Taq™ (TakaRa) using a 7500 real-time PCR system (Applied Biosystems Inc.) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR was performed according to the the manuals of PrimeSTAR Max Premix (2X) (TaKaRa, R045) or 2×EasyTaq PCR SuperMix (+ dye ...
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bioRxiv - Plant Biology 2020Quote: Generation of expression constructs was performed with both In-Fusion Advantage PCR Cloning Kit (Clontech) and Gateway Cloning Technology (Invitrogen).
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bioRxiv - Plant Biology 2020Quote: ... The PCR products were amplified from genomic DNA using PrimeSTAR® Max DNA Polymerase (TaKaRa). A restriction digest was performed using BglII and BstEII (NEB ...
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bioRxiv - Neuroscience 2020Quote: Long-range PCR was performed using PrimeSTAR® GXL DNA Polymerase (R050A) (Takara, Shiga, Japan). A 10,143 kb fragment was amplified by PCR in a thermal cycler (TaKaRa PCR Thermal Cycler ...
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bioRxiv - Microbiology 2021Quote: ... were prepared by PCR and the In-Fusion cloning system (Takara Bio, Kusatsu, Shiga, Japan). The inserted DNA sequence of each vector was confirmed by direct DNA sequencing ...
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed using a One Step PrimeScript RT-PCR Kit (Takara Bio, Otsu, Japan), and the following real-time PCR conditions were applied ...
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bioRxiv - Microbiology 2019Quote: All PCR reactions were performed using Prime STAR GXL DNA Polymerase (Takara Bio, Otsu, Japan). The pCold™ II (Takara Bio ...
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bioRxiv - Physiology 2021Quote: ... PCR was performed with cDNA derived from 0.5 μg of total RNA with Extaq (TaKaRa). The primer sequences were 5’-CAACGCCCATCGTTTCATGG-3’ and 5’-CAAAAACTTGAAGCCCGCCA-3’ for NtGAox20 and 5’-ATGTGTTCGTTTCAGCCCGA-3’ and 5’-CCGCTGAAAAGTGTGCTTCC-3’ for tobacco arcA as an internal gene control (Ishida et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Youren RT-PCR products were cloned into the pENTR™/D-TOPO™ vector (Takara) and sequenced by Sanger sequencing (Applied Biosystems 3500).
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bioRxiv - Neuroscience 2020Quote: ... The cDNA was then amplified using the Advantage 2 PCR Enzyme System (Takara, Fremont, CA) for 6 cycles ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting PCR product was recombined using in-Fusion HD cloning plus CE (Clontech, 638916) to create a functional circular plasmid ...
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bioRxiv - Microbiology 2019Quote: ... Specific PCR products were excised from 2% agarose gel and cloned into pMD19-T (TaKaRa) and then sequenced ...
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bioRxiv - Microbiology 2020Quote: ... consisting of 20 μL Emerald AMP GT PCR 1X Master Mix (Takara Bio, Shiga, Japan), 0.5 μL (10 μM ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time PCR was performed using SYBR-Green based fluorescence detection kit (TAKARA, Japan) and HT9700 detection system (AB ...
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bioRxiv - Cancer Biology 2021Quote: High-throughput quantitative PCR (qPCR) was performed using the SmartChip system (Takara Bio, Kusatsu, JAPAN) on 47 oral melanoma samples to detect focal amplifications on tumor DNA on CFA 10 and CFA 30 ...
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bioRxiv - Biochemistry 2022Quote: ... the cDNA of PFK1-mEGFP was amplified by PCR and cloned into pTRE3G vector (Clontech). The pTRE3G vector containing PFK1-mEGFP was then co-transfected into HeLa Tet-On 3G cells (Clontech ...
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bioRxiv - Molecular Biology 2022Quote: The Delta PAS sfRps29 vector was generated by PCR using PrimeSTAR MAX Polymerase (Takara Bio) with primers containing the point mutation (forward ...
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bioRxiv - Molecular Biology 2022Quote: ... Long range PCR reactions were performed using the Takara LA Taq polymerase (Takara Bio, RR002M), with 250 ng genomic DNA ...
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bioRxiv - Pathology 2022Quote: ... PCR reactions were conducted with SYBR qPCR Premix Ex Taq II Tli RNaseH+ (TAKRR820W,Takara). Primer pairs are listed in Table S2 ...
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bioRxiv - Neuroscience 2022Quote: ... We inserted the PCR fragments at NheI/BamHI sites by infusion HD-cloning (Takara Biosciences), to excise HA-tag from the plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was performed to detect mRNA expression of genes by TB Green PCR Kit (TaKaRa) and primers are shown in table S2.
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bioRxiv - Neuroscience 2022Quote: ... For first-strand cDNA synthesis the Takara PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan) was used with 50 μM Oligo (dT ...
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed using SYBR Premix EX Taq (Tli RNaseH Plus) (Takara) in a QuantStudio3 thermal cycler (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... PCR reactions were carried out using Hot Start TaKaRa LA Taq kit (Takara Biotechnology, #RR042A) with the following cycling conditions ...
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bioRxiv - Plant Biology 2021Quote: ... the clones were used as a template for PCR using PrimeSTAR MAX (TaKaRa, Kusatsu, Japan); the primers are listed in Table S2 ...