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Citations for Takara Bio :
1001 - 1050 of 2305 citations for 6H Purin 6 one 2 amino 8 chloro 1 9 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and E484Q mutations of the SARS-CoV-2 spike protein were determined by qPCR using mutation detection kits purchased from Takara Bio (Shiga ...
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bioRxiv - Plant Biology 2022Quote: ... backbone with the FLAG and SV40 NLS (for version1) or without the FLAG and SV40NLS (for version 2) were amplified and assembled by TAKARA in-fusion HD cloning kit (cat639650 ...
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bioRxiv - Microbiology 2022Quote: ... Ct values were obtained from PCR tests conducted as administrative tests at the Toyama Institute of Health using the SARS-CoV-2 direct detection RT-qPCR test kit from Takara Bio Inc ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using QuantStudio Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... The genomic DNA sequences surrounding the potential off-target sites selected for further analysis (Table 2) were amplified by PCR using specific primers (Supplementary Table S1) and PrimeSTAR GXL DNA Polymerase (Takara) from CRISPR/Cas-edited hairy roots (R1 of E-CP1 [the main root and five lateral roots] ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells and hippocampal neurons were transfected with plasmids encoding FM4 recombinant receptors for 15 h and then treated with 2 μM DD-Solubilizer (TakaraBio/Clontech) to induce the release of the receptors from the ER into the secretory trafficking ...
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bioRxiv - Microbiology 2024Quote: Each individual SARS-CoV-2 fragment was amplified from their respective plasmids using an exclusive pair of primers (Supplementary Table 2) and high-fidelity PrimeSTAR GXL DNA polymerase (Takara), followed by gel isolation with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids expressing HuSaV (GI.2) and PSaV protease-polymerase fusion were generated by cloning into the XhoI and BamHI sites of pmCherry-C1 (Clontech). Vectors expressing RIG-I-CARD domain [25] and IRF3-5D [26] were previously described ...
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bioRxiv - Immunology 2024Quote: ... T cells were activated for 2 days and NK cells were activated for 4 days followed by retronectin (Takara, T100B) mediated viral transduction ...
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bioRxiv - Microbiology 2024Quote: ... individual FLAG-HA tagged Vpr genes were amplified from the pLRGatewayIRESeYFP-Vpr plasmids using the primers in Table 2 and subcloned into pLVX-TetOne-puro (Clontech) using EcoRI and BamHI restriction sites ...
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bioRxiv - Microbiology 2024Quote: ... Y453F and T76G were inserted in plasmid F5 or F1 respectively by Directed Site Mutagenesis using the primers (Supplementary Table 2) and high-fidelity PrimeSTAR GXL DNA polymerase (Takara).
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bioRxiv - Neuroscience 2023Quote: Mammalian expression constructs were mainly generated by Gibson assembly of PCR-amplified coding sequences (primers, Supp. Data File 2) into restriction-digested pC1 (CMV promoter, modified from pEGFP-C1, Clontech) or pCAG (CMV enhancer fused to chicken beta-actin promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... The sequence spanning the I-SceI cleavage site in the SceGFP gene was amplified from 150 ng of genomic DNA using Advantage 2 Polymerase (Clontech) with primers dr-for (5’-CCCGCCACCTGCCCCATCTGCTA ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: SseI gene was amplified from Salmonella typhimurium (STM) strain LT-2 strain using high fidelity DNA polymerase enzyme from TaKaRa and cloned in with N-terminal flag and HA tag in mammalian expression vector pcDNA3 flag HA Akt1 plasmid obtained from addgene(1477) ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... A total of 3.5 μl ribo-depleted material was used for SMARTer (SMARTer PCR cDNA Synthesis Kit, catalog num. 634926 and Advantage 2 PCR kit, catalog num. 639207, Clontech-Takara) protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysate was then filtered through a 0.45 µm syringe filter and mixed with 2 mL of washed TALON® Metal Affinity Resin beads (Takara). The mixture was allowed to bind under nutation at 4℃ for 1 hr ...
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... was used as a template to generate the pCMV-myc-SFPQ ΔRRM1-2 by mutagenesis using the InFusion HD kit from Clontech (catalog number 639649 ...
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bioRxiv - Genetics 2023Quote: ... cDNA was generated from 2 ng of RNA using the Smart-Sq V4 Ultra Low Input RNA Kit (Takara, 634894). Next ...
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bioRxiv - Genomics 2023Quote: ... The transfected cells were cultured for 2 days and lentivirus were harvested and concentrated using the Lenti-X concentrator (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized with 2 μg RNA using PrimeScript™ 1st strand cDNA synthesis kit (TaKaRa, Kusatsu, Shiga, Japan) in a final reaction volume of 20 μl following the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... and GC B cells (live singlet CD19+ CD4− IgDlo CD71+CD38int CD20+ CXCR5+) were sorted using a FACSAria II into 96-well plates containing 2 μL Lysis Buffer (Clontech) supplemented with 1 U μl−1 RNase inhibitor (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... Fractionated CD34+ cells from Animals #2 and #3 were cultured overnight on RetroNectin-coated plates (Takara, T100B, Mountain View, CA) in X-VIVOTM 10 (Lonza ...
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: The dCas9-KRAB-NLS-NLS sequence was amplified from the plasmid PB-TRE-dCas9-KRAB-MeCP2 (Tan, 2022) (addgene 122267) using PrimeSTAR Max 2 ×premix (Takara). A T7 promoter sequence was included in forward primer for in vitro transcription using the mMESSAGE mMACHINE T7 Ultra Transcription kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The upstream and downstream regions adjacent to the binding site were amplified using Advantage 2 polymerase (Takara Bio, Shiga, Japan) from 3T3-L1 genomic DNA and cloned into the HR110-PA-1 vector (System Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized using a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara).
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bioRxiv - Molecular Biology 2024Quote: ... AAV titer was determined by qPCR method using AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara-bio, #6233) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... The plasmids were used to transform competent yeast cells using YeastMaker Yeast Transformation System 2 (Takara, Mountain View, CA94043, USA), and the binding was validated in synthetic dropout-Leu-Trp-His-Ade medium supplemented with X-α-gal ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from RNA (approximately 2 μg) with oligo(dT) primers according to the instructions of the PrimeScript First Strand cDNA Synthesis Kit (Takara).
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice and single rEtpA-binding PBs (rEtpA+ CXCR5low CD71+ CD20low CD38+ IgDlow CD19+/int CD4− CD8− CD14− live singlet lymphocytes) were sorted using a Bigfoot into 96-well plates containing 2 µL Lysis Buffer (Clontech) supplemented with 1 U/µL rNase inhibitor (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice and single PBs (CD138+ CD38lo CD19+/lo CD3- live singlet lymphocytes) were sorted using a FACSAria II into 96-well plates containing 2 µL Lysis Buffer (Clontech) supplemented with 1 U/µL rNase inhibitor (NEB ...
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bioRxiv - Molecular Biology 2024Quote: Plasmids encoding SARS-CoV-2 NSP1 variants were generated by exchanging the EGFP coding sequence of pEGFP-N1 plasmid (Clontech) for the corresponding nsp1 genes ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies: anti-GFP (chick 1:20) and anti-DsRed (rabbit, 1:50, Takara Bio#632496). Secondary antibodies ...
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bioRxiv - Genetics 2024Quote: ... Aliquots of these samples were diluted 1:10 and 1:100 on two plates (Takara Bio): (1 ...
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bioRxiv - Genetics 2024Quote: ... Aliquots of these samples were diluted 1:10 and 1:100 on three plates (Takara Bio): (1 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-dsred (1/1000; TaKaRa), mouse anti-acetylated Tubulin (1/500 ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-DsRed (1:100, Clontech).
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bioRxiv - Physiology 2021Quote: ... and DSRed (1:1000, #632392, Clontech) antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... rabbit anti-DsRed (1:250; Clontech), mouse anti-ratCD2 (OX-34 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-GFP (Clontech, 1:5000) rat anti-RFP (Chromotek ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 U μl–1 SMARTScribe (Clontech) and 10 U μl–1 RNASin plus (Promega) ...
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bioRxiv - Neuroscience 2019Quote: ... Rabbit anti-Dsred (1:250, Takara Bio ...
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bioRxiv - Neuroscience 2019Quote: ... rabbit anti-DsRed (1:500; Clontech), mouse anti-rCD2 (OX-34 ...
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bioRxiv - Neuroscience 2019Quote: ... rabbit anti-DsRed (1:1000, Clontech), mouse mAb anti-ChAT (1:100 ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-RFP (1:1,000, Clontech), mouse monoclonal anti-Bruchpilot ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 U/μl RNase inhibitors (TaKaRa)) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 µg/mL of puromycin (Clontech) was added to cells for 2 weeks ...