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Citations for Takara Bio :
901 - 950 of 5356 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and ligated with a DNA ligation kit (Takara). The constructs expressing GFP-BDI-bK6A and single amino acid substitutions in the SLiM sequence were generated by PCR-based site-directed mutagenesis using oligonucleotides and templates listed in Table S1 and inserted into the SalI site of pHE-C or phsp-GFP-Halo as described above ...
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bioRxiv - Bioengineering 2023Quote: ... Using a BCA protein kit (TaKaRa, Shiga, Japan), a working solution (BCA reagent A ...
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bioRxiv - Biochemistry 2023Quote: ... using the In-Fusion HD Cloning kit (Takara) to generate NanoLuc fusion on the N-terminus of NSD2 ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... using in-fusion cloning kit (Takara, Kusatsu, Japan). To generate pFA6a-link-GBP-CaURA3 ...
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bioRxiv - Genetics 2023Quote: ... and DNA Unique Dual Index Kit (Takara, R400666). Paired-end sequencing of ChIP-seq libraries was performed (150 bp ...
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bioRxiv - Microbiology 2023Quote: ... Protoplasts were stained following ApoAlert kit indications (TaKaRa) and the ratio of apoptotic cells was measured by flow cytometry ...
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bioRxiv - Cancer Biology 2024Quote: ... employing the CalPhos mammalian transfection kit (Takara Bio). Supernatants were harvested at 48 and 72 h post-transfection and subsequently stored at −80°C.
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bioRxiv - Plant Biology 2024Quote: ... with an In-Fusion Cloning Kit (Clontech Laboratories). Plasmid DNA was purified using a ZR Plasmid Miniprep-Classic Kit (Zymo Research ...
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bioRxiv - Plant Biology 2024Quote: ... The Prime Script TM RT reagent Kit (TAKARA) was used to synthesize the first-strand cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... The In-Fusion HD cloning kit (Takara 638909) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... or SYBR Premix Ex Taq II Kit (Takara) with gene-specific primers (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2024Quote: ... the In-Fusion HD Cloning kit (Takara Bio) was used to replace the sequence for puromycin resistance with the sequence of the Blue Fluorescent Protein (tagBFP) ...
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bioRxiv - Plant Biology 2024Quote: ... using In-Fusion HD Cloning Kit (Takara Bio). Amino acid substitutions were introduced into pMAL-c5X MAP4K1 or MAP4K2 by site-directed mutagenesis to produce the kinase-dead form of MAP4K1K44N or MAP4K2K44N and the non-phosphorylatable form of MAP4K1K44N/S479A or MAP4K2K44N/S488A ...
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bioRxiv - Plant Biology 2024Quote: ... using a SYBR Green RT-PCR kit (Takara) and a Roche Light Cycler 480 ...
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bioRxiv - Plant Biology 2024Quote: ... with the SYBR Premix Ex-Taq Kit (Takara). EF-1α (AT1G18070 ...
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bioRxiv - Plant Biology 2020Quote: The total RNA of the buds collected for the transcriptome research was extracted by the EASYspin Plus Plant RNA Kit RN37 (Aidlab Biotechnology) and cDNA synthesis by the M-MLV RTase cDNA Synthesis Kit (TaKaRa Company). cDNA amplified fragment length polymorphism (cDNA-AFLP ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from DRG was extracted using RNeasy micro kit and reverse-transcribed using PrimeScript RT Reagent Kit (Cat# RR037A, TaKaRa, Japan). Real-time PCR was performed using the SYBR Premix Ex Taq™ (Cat# DRR041A ...
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bioRxiv - Biochemistry 2019Quote: ... cDNAs were synthesized from the isolated RNA using a cDNA synthesis kit (Advantage RT-PCR kits) from Clontech (Mountain View, CA). Specific primers designed according to human ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells by TRIzol and an RNA extraction kit (MiniBEST Universal RNA Extraction Kit, RR820A, Takara, China). Total RNA (1 μg ...
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bioRxiv - Genetics 2020Quote: RNA was extracted using the Biostic Blood Total RNA Isolation Kit sample (MO BIO laboratories, Inc) and cDNA was obtained using the PrimeScript™RT reagent Kit (Takara). RTqPCR gene expression analysis was performed using the Taqman probes HSF2-Hs00988309_g1 and GADPH-Hs02758991_g1 for normalization (Applied Biosystems).
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Engineering of membrane complex sphingolipids improves osmotic tolerance of Saccharomyces cerevisiaebioRxiv - Bioengineering 2019Quote: Total RNA was extracted using MiniBEST universal RNA extraction kit and 1 μg was taken to synthesize cDNA using the PrimeScript II 1st-strand cDNA synthesis kit (TaKaRa, Japan). The cDNA mixture was diluted to about 100 ng/μl and used as the template for the gene expression level analysis by qRT-PCR ...
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bioRxiv - Physiology 2021Quote: ChIP-seq libraries were prepared on 5 ng of DNA from ChIP and input samples using the SMARTer ThruPlex DNA-seq kit along with DNA HT Dual Index Kit (both from Takara Bio) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was extracted directly from the virus stock using the Indimag Pathogen kit (Indical Biosciences) and transcribed to cDNA using the PrimeScript™ RT reagent Kit (Takara) using oligo-dT and random hexamers ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was isolated using the FastGene Plasmid Mini Kit (Nippon Genetics, Tokyo, Japan) or NucleoSpin Plasmid EasyPure Kit (Takara Bio). The REase NdeI was employed for the linearization of plasmid DNAs ...
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bioRxiv - Physiology 2024Quote: cDNA synthesis and Real-Time PCR were performed following the manufacture instructions of the PrimeScript RT reagent Kit with gDNA Eraser kit (Takara, #RR047A). For the Real-Time PCR reaction ...
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bioRxiv - Neuroscience 2024Quote: Total RNAs from scramble shRNA or TDP-43 shRNA treated cells were used to construct RNA-sequencing libraries using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit (Takara 634411), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Whole genome amplification was performed on the prepared mixture samples with BioSkryb ResolveDNA™ Whole Genome Amplification Kit (BioSkryb Genomics) and Takara PicoPlex WGA v3 kit (Takara) following the manufacture’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... and CDS of fluorescent proteins were subcloned into pET-human αSyn51 using the KOD-Plus Mutagenesis kit (TOYOBO) and In-Fusion HD Cloning Kit (Takara Bio) according to the manufacturers’ protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The viral particles were purified from transfected cells and titrated using the AAVpro Purification Kit Maxi and AAVpro Titration Kit (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were prepared using SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian kit (Takara Bio USA, Cat.# 634411) and following the user manual (Rev ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using “SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian” kit (Takara Bio USA, Cat. # 634411), following “SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian User Manual (Rev ...
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bioRxiv - Molecular Biology 2024Quote: RNA that passed the quality test underwent RNA polyadenylation and cDNA synthesis according to the instructions of the kit manufacturer (Mir-X™ miRNA First-Strand Synthesis Kit; Takara). The reaction system was configured according to the real-time fluorescence quantitative PCR user manual (ChamQ Universal SYBR® qPCR Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using “SMARTer Stranded Total RNA-seq Kit v2 – Pico Input Mammalian” kit (Takara Bio USA, Cat. #634411). The protocol was started with 4-10 ng of total RNA ...
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bioRxiv - Developmental Biology 2022Quote: c-Kit-EGFP fusion protein: chicken c-Kit cDNA was inserted into multiple cloning site of pEGFP-N1 Vector (Clontech, #6085-1) including EGFP sequences using a primer set ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were induced by 1 mM IPTG at 16°C for 20 h, then purified by a GST-tag Protein Purification Kit (Beyotime, Shanghai, China) or His TALON Purification Kit (Takara, Beijing, China). Corresponding primers are listed in Supplemental Table S2.
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted with an Axygen RNA purification kit (Capital Scientific) and the first DNA was synthesized from RNA using a PrimeScript RT regent kit (Takara, Shiga, Japan). Actin-coding gene (KP341672 ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... RNA was reverse-transcribed to cDNA by using DNA removal and a cDNA synthesis kit (PrimeScriptTM RT reagent Kit with gDNA Eraser; TaKaRa, Kusatsu, Japan) with a mixture of oligo-dT and random primers ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was removed using the DNA-free kit (Thermo Fischer Scientific, Cat# AMl906) and subsequent cDNA synthesis was performed using the PrimeScript RT reagent Kit (TAKARA, Cat# RR037A), both according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was collected using the Nucleospin RNA Kit (Macherey–Nagel, Hoerdt, France) and transcribed into complementary DNA (cDNA) with the PrimeScript RT Reagent Kit (RR037A, Takara, Kusatsu, Japan). cDNA was then amplified with the SYBR Green Master kit (Roche ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted using the nucleospin RNA kit (Takara) according to the manufacturer’s recommended protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... SMART-Seq v4 Ultra Low input RNA kit (Clontech) was used to amplify cDNA which was subsequently sheared resulting in 200-500 bp fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was generated using PrimeScript RT kit (Takara #RR037A). For semi-quantitative RT-PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... using a Random Primer DNA Labeling Kit (TaKaRa, 6045) according to the manufacturer’s protocol ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... coli using the NucleoSpin Plasmid miniprep kit (Takara 740588). Transformants were sequenced to confirm successful cloning ...
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bioRxiv - Immunology 2021Quote: ... The SMARTer ThruPLEX DNA-Seq kit (Takara, Rockville, MD) was utilized to generate libraries from 10 μl of sheared cDNA template ...
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bioRxiv - Molecular Biology 2021Quote: ... using the In-Fusion® HD Cloning Kit (Takara). The subsequent mutations in the TCRA and TCRB 3’-UTRs were generated using these initial constructs ...
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bioRxiv - Molecular Biology 2021Quote: ... and CalPhos transfection kit according to manufacturer’s instructions (Takara). 2 days after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... using the IN-Fusion HD EcoDry Cloning Kit (Clontech).
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bioRxiv - Biochemistry 2019Quote: ... A SYBR Premix Ex Taq kit (TaKaRa, Dalian, China) was used to perform quantitative PCR on a CFX96 Real-Time PCR detection system (BioRad) ...
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bioRxiv - Physiology 2020Quote: ... and titered with Adeno-X Rapid Titer Kit (Clontech). Adenoviruses were delivered into mice intravenously at 5 × 108 pfu/mouse for overexpression experiments ...