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Citations for Takara Bio :
901 - 950 of 2499 citations for PCR Strips since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... DNA fragments containing eIF4A iso4 were PCR-amplified and inserted into pColdI (TaKaRa) downstream of the His tag with In-Fusion HD (TaKaRa) ...
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bioRxiv - Bioengineering 2019Quote: ... and Cd200 were amplified by extension PCR using PrimeStar HS mastermix (Takara R040) and gateway compatible attb1/attb2 sites were added to the 5’end and 3’ ends ...
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bioRxiv - Genomics 2019Quote: ... The IRES2 fragment was PCR amplified from the pTRE3G-IRES (Cat. # 631174, Clontech) using the primer pairs
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bioRxiv - Evolutionary Biology 2019Quote: ... These PCRs were performed with the Advantage2 Taq Polymerase (Clontech, Mountain View, CA) for high fidelity ...
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bioRxiv - Genetics 2019Quote: ... Real-time PCR was performed using a SYBR Premix Ex TaqTM kit (TaKaRa) on an ABI prism 7500 real-time PCR System and three biological repeats ...
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bioRxiv - Neuroscience 2019Quote: ... Physical viral titer was determined using Lenti-X qRT-PCR Titration Kit (Takara), and only viruses greater than 1×1011 GC/ml were used ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The PCR products were directly ligated into PMD19-T simple vector (TaKaRa, China) and then transformed into Escherichia coli BL21 competent cells (Transgen ...
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bioRxiv - Biochemistry 2019Quote: ... Any additional plasmids were constructed using backbone or insert PCR and inFusion (Clontech). Stellar competent cells were transformed by all the plasmids for amplification and DNA storage.
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bioRxiv - Genetics 2021Quote: ... The BE2+Cas9m4 insert was PCR amplified and cloned using in-Fusion (Clontech) into the pActin5c-attB-RFP vector isolated by digestion of pActin5c-Cas9 (Addgene# 62209 (Port et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative RT-PCR was performed using TB Green Premix Ex Taq II (Takara). Relative gene expression was normalized to ACTB expression ...
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bioRxiv - Microbiology 2021Quote: ... The gene quantitative PCR was performed using TB green Premix EX Taq (Takara) with a QuantStudio 6 Flex (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed with PrimeSTAR Max DNA polymerase (Takara Bio Inc, Shiga, Japan) with the following parameters ...
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bioRxiv - Microbiology 2020Quote: ... and 2 µl of lysate was used for PCR using SapphireAMP (Takara, RR350) and gene-specific primers ...
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bioRxiv - Synthetic Biology 2020Quote: ... Polymerase chain reaction (PCR) fragments were generated using PrimeSTAR Max DNA Polymerase (TaKaRa). The method for plasmid construction is described below.
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bioRxiv - Bioengineering 2021Quote: Polymerase Chain Reactions (PCR) were conducted using PrimeSTAR GXL DNA Polymerase (Takara Bio) per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... FveGID1c were PCR amplified and ligated into GAL4 AD vector PGADT7 (Clontech Inc.) at the BamHI/XhoI ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR reaction included 2.5 µL of 10X buffer (Mg2+ plus) (TaKaRa, Japan), 1.5 µL of 25 mM MgCl2 ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with the Takara ExTaq (Takara, Kusatsu, Japan) reaction kit ...
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bioRxiv - Genetics 2021Quote: ... Quantitative RT-PCR was performed by using SYBR Premix Ex TaqTM (TaKaRa Bio) on a ABI StepOnePlusTM Real-Time PCR thermocycler (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... Quantitative RT-PCR was performed by using SYBR Premix Ex TaqTM (TaKaRa Bio) on an ABI StepOnePlusTM Real-Time PCR thermocycler (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... PCR products were used for sub-cloning into the pMD18-T vector (Takara) and sequenced to determine mutation efficiency ...
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bioRxiv - Microbiology 2021Quote: ... then ligating both PCR products by In-Fusion (Takara Bio, Mountain View, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... BacT/ALERT 3D Microbial Identification System and PCR Mycoplasma Detection Set (Takara, 6601) were applied to detect bacterial/fungi and mycoplasma contamination ...
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bioRxiv - Neuroscience 2021Quote: ... One microgram of cDNA was added to the PCR-reaction premix (Takara Bio) with 10 pM corresponding primer pairs ...
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bioRxiv - Cell Biology 2020Quote: ... by PCR and inserted to vectors peGFP-C2 and pmRFP1-C2 (originally Clontech), designed for expression of protein chimeras with a fluorescent protein connected to the N-terminus of the target protein ...
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bioRxiv - Biophysics 2020Quote: ... The mCherry gene (AgeI / NheI) was obtained by PCR on pmCherry-N1 (Clontech). A four-fragment-ligation was then done to obtain the final construct (HindIII-HA-Nlg1-AgeI-mCherry-NheI-Nlg1Cter-NotI) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Long-range PCRs were performed with PrimeSTAR GXL DNA Polymerases (Takara Bio Europe). Initial sequences generated following Bourguignon et al ...
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bioRxiv - Plant Biology 2021Quote: ... the coding regions of RSL4 (AT1G27740.1) were amplified by PCR (PrimeStar max, Takara) using genomic DNA and cDNA as templates ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with 2x SeqAMP buffer and SeqAmp DNA polymerase (Takara USA) and for the final amplification ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with 2x SeqAMP buffer and SeqAmp DNA polymerase (Takara USA) and for the final amplification ...
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bioRxiv - Microbiology 2021Quote: ... using random hexamers and PCR was performed with PrimeSTAR Max DNA Polymerase (Takara). Primers were designed based on the assembled viral contigs ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was accomplished using the SYBR premix Ex Taq kit (Takara, China) on an ABI StepOnePlus Real-Time PCR System (Thermofisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Nested PCR was performed using TaKaRa Ex Taq (Takara Bio Inc., Shiga, Japan). The initial and nested PCR protocols have been described elsewhere(46 ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed using One Step PrimeScript RT-PCR Kit (Takara, Japan) with the following primers and probes ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was reverse-transcribed using mRNA Selective PCR kit (TaKaRa Bio-Clontech). Mouse integrin beta 1 ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was reverse-transcribed using mRNA Selective PCR kit (TaKaRa Bio-Clontech). Mouse integrin beta 1 ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplifications were carried out with Phusion polymerase (Finnzymes) or PrimestarMAX (Takara Clontech) as recommended by the manufacturers ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplifications were carried out with Phusion polymerase (Finnzymes) or PrimestarMAX (Takara Clontech) as recommended by the manufacturers ...
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bioRxiv - Developmental Biology 2020Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq II (Takara Bio) on an ABI 7500 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using One Step PrimeScript RT-PCR Kit (Takara, Japan) with the following primers and probes 55 ...
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bioRxiv - Genetics 2020Quote: ... PCR amplification was performed with TaKaRa LA Taq Hot Start polymerase (TaKaRa Biotechnology) under conditions of ...
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bioRxiv - Microbiology 2020Quote: ... Following PCR amplification with TaKaRa Ex Taq DNA Polymerase (ClonTech, Mountain View, CA), the PCR product and pRRK plasmid were digested with the XBaI restriction endonuclease (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... The ORF3b derivatives were generated by PCR using PrimeSTAR GXL DNA polymerase (Takara), the synthesized ORFs as templates ...
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bioRxiv - Synthetic Biology 2022Quote: ... PrimeStar DNA polymerase and SapphireAmp* Fast PCR Master Mix were purchased from TaKaRa Bio ...
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bioRxiv - Cancer Biology 2024Quote: ... and first-strand cDNA was prepared using an AMV RNA PCR kit (TaKaRa) from 1 mg of total RNA ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed using the TB Green Ex Taq II Mix (Takara Bio) and the Thermal Cycler Dice Real-Time System (Takara Bio).
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bioRxiv - Neuroscience 2022Quote: ... DNA was purified using NucleoSpin Gel and PCR Clean-up kits (Takara, 740609.250). Libraries were prepared identically to SHARE-seq ATAC libraries.
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bioRxiv - Microbiology 2022Quote: ... This cassette was further modified by PCR and circularized by recombination (InFusion Takara) to replace gRNA1-tracerRNA1-gRNA2 by a NotI site ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was then reverse transcribed by the SMARTer PCR cDNA Synthesis Kit (Clontech) and amplified for 15 cycles using KAPA HiFi PCR Kit (KAPA biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 µl of lysate was used for PCR using SapphireAMP (Takara, RR350) and primers specific for each gene ...