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Citations for Takara Bio :
9401 - 9450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Purified DNA was directly ligated with pMD18-T vector (Takara) for TA cloning according to the pMD18-T kit protocol ...
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bioRxiv - Microbiology 2022Quote: ... This cassette was further modified by PCR and circularized by recombination (InFusion Takara) to replace gRNA1-tracerRNA1-gRNA2 by a NotI site ...
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bioRxiv - Microbiology 2022Quote: ... Vectors for expression of mcp were generated using the pEF1-mRFP-NAT vector by InFusion (Takara) into the NdeI or XhoI site for N- or C-terminal RFP tagging ...
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bioRxiv - Microbiology 2022Quote: ... and inserted into the NotI site by InFusion (Takara).
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bioRxiv - Biochemistry 2022Quote: ... the SYBR Premix Ex Taq II (Takara Bio) was used for quantifying the specific transcripts and analyzed with QuantStudio 6 Flex (Life Technologies) ...
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bioRxiv - Biochemistry 2022Quote: ... For reverse transcription using Primescript RT reagent kit (Takara Bio Inc.), 1 μg of extracted RNA was used according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... we co-transfected low-passage A549 cells with pIFNB1-Luc or pNFκB-Luc and a linear hygromycin DNA fragment flanked by the SV40 promoter and polyadenylation signal (Clontech Laboratories, Inc.) by using the TransIT LT-1 transfection reagent (Mirus Bio LLC) ...
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bioRxiv - Biochemistry 2022Quote: ... 100pmols of Cas9 (Takara) was incubated at room temperature for 10 minutes with 200pmols of sgRNA (Synthego) ...
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bioRxiv - Immunology 2022Quote: TCR repertoire profiling was performed using the SMARTer TCR α/β Profiling Kit (Takara Bio, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... downstream of the His tag with In-Fusion HD (TaKaRa). The Gly172Phe substitution was induced by site-directed mutagenesis.
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bioRxiv - Molecular Biology 2022Quote: ... downstream of the His tag with In-Fusion HD (TaKaRa). The His154Gly substitution was induced by site-directed mutagenesis.
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bioRxiv - Molecular Biology 2022Quote: ... sinensis eIF4A1 gene were synthesized by Integrated DNA Technologies (IDT) and inserted into pColdI (TaKaRa) downstream of the His tag with In-Fusion HD (TaKaRa) ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR product thus obtained was cloned using the In-Fusion HD Cloning Plus kit (Takara Bio – 638909) into the pMA_F3NeoLoxPPolyFRT vector provided by Dr ...
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bioRxiv - Developmental Biology 2022Quote: ... 50⍰ng of pLacZ-Control Vector (a transfection control, Clontech – 631709) and increasing concentrations of mouse miR-483-3p mimic (0 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was then reverse transcribed by the SMARTer PCR cDNA Synthesis Kit (Clontech) and amplified for 15 cycles using KAPA HiFi PCR Kit (KAPA biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 volume of Lenti-X Concentrator (TAKARA, 631231) was added to 3 volumes of supernatant and incubated at 4°C for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription was performed by using PrimerScriptTM RT Master Mix Kit (TaKaRa) according to directions ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was performed using SYBR Premix ExTaq™ (TAKARA, Japan) by StepOnePlus Real-time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara-Bio) and the following primers ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using QuantStudio Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... coli Stellar (Takara Bio) cells and plated on selective Luria-Bertani (LB ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-E-cadherin rat monoclonal antibody (Takara, M108, 1:500), anti-GFRα1 goat polyclonal antibody (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized by reverse transcription using the Prime Script RT reagent kit (TaKaRa) and subjected to quantitative RT-PCR with Setd2 and target gene primers in the presence of the SYBR Green Realtime PCR Master Mix (Thermo) ...
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bioRxiv - Molecular Biology 2022Quote: ... TB Green Premix Ex TaqTM Ⅱ Kit (TaKaRa) was applied in determining the abundance of mRNA according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2022Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara-Bio)] and incubated at room temperature for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... 1 ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was applied onto a pre-equilibrated column containing Protino Glutathione Sepharose™ 4B beads (Takara) and kept for binding for 3 hrs at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... were mixed to a final volume of 150 µL and incubated at room temperature for 30 min before adding to the Lenti-X™ 293T Cell Line (Takara, Cat. #: 632180). Media (10% FBS ...
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bioRxiv - Biophysics 2022Quote: ... containing virus was collected after 48 hrs and concentrated by 50-fold following the protocol of Lenti-X™ Concentrator (Takara, Cat. #: 631232). The concentrated virus in 40uL volume was added to HEK293T cells plated several hours ahead started with 80,000 cells in one well of a 24-well plate ...
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bioRxiv - Bioengineering 2022Quote: ... Capturem large volume filters (Takara Bio USA Inc., San Jose, CA, USA) were used to estimate the quantity of membrane required ...
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bioRxiv - Cancer Biology 2022Quote: ... Both cell lines were initially infected with the pLVX-Tet3G (Clontech) regulator vector and then underwent selection with Geneticin (1 mg/mL) ...
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bioRxiv - Cancer Biology 2022Quote: ... Surviving cells were then infected with lentivirus for either mitoGFP or NDI1 expressed in the pLVX-TRE3G (Clontech) response vector and selected with puromycin (2 μg/mL).
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bioRxiv - Cancer Biology 2022Quote: ... and 20 post infection using a NucleoSpin kit (Takara). PCR was performed as previously described and samples were sequenced on a MiSeq sequencer (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... a DNA fragment was PCR-amplified from human genomic DNA using Tks Gflex DNA Polymerase (#R060A, Takara Bio) and the primers listed in Table S1 ...
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bioRxiv - Cell Biology 2022Quote: ... sites using the In-Fusion HD Cloning Kit (#Z9648N, Takara Bio), yielding entry plasmids pENTR4XE153 ...
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bioRxiv - Cell Biology 2022Quote: ... The virus titer (ifu/ml) defined by Clontech’s Lenti-X qRT-PCR Titration Kit (Cat ...
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bioRxiv - Cell Biology 2022Quote: ... containing a Kozak sequence added right before the ATG (RefSeq NC_045512.2) were synthesized by Eurofins (Ebersberg, EU) and subcloned into the pIRES2 vector with eGFP in the second cassette (Takara Bio Europe, EU). Truncated Δ4 and Δ8 E proteins ...
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bioRxiv - Cell Biology 2022Quote: ... as described previously.15 Alkaline phosphatase (ALP) staining was also performed for pluripotency characterization using TRACP & ALP double-stain Kit (Takara) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Nuclei were isolated from 1.5 × 109 DT40 cells and digested with 60 units/ml MNase (Takara Bio Inc.) in buffer A (15 mM Hepes-KOH ...
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bioRxiv - Cell Biology 2022Quote: ... and viral particles were precipitated using Lenti-X concentrator (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized with 1 μg of total RNA using the Prime ScriptTM RT Reagent Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... using SYBR® Premix Ex TaqTM II (Takara) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Produced lentiviruses were concentrated 100x with Lenti-X Concentrator (#631232, Takara) to increase virus concentration ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was filtered and concentrated with LentiX™ Concentrator reagent following manufacturer’s instructions (Clontech). Primary neurons were infected at DIV 7 and after 24 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequences derived from pRetroX-G1-Red and pRetroX-SG2M (Clontech) were cloned into pHR lentiviral vectors under puromycin or blasticidin selection ...
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bioRxiv - Immunology 2022Quote: ... the same sequences were cloned into the pQCXIP vector backbone (Takara Bio, Mountain View, CA) with a C-terminal Myc tag ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 100 ng of total RNA was used as input for RNA- seq libraries using SMARTer Stranded Total RNA-seq - Pico input (Takara Bio USA, Inc.) to remove rRNA transcripts ...
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bioRxiv - Cancer Biology 2022Quote: ... The PCR products were purified from agarose gels using NucleoSpin Gel and PCR Clean-Up Kit (740609, Clontech) and subcloned into the pENTR/D-TOPO vector (K240020 ...
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bioRxiv - Neuroscience 2022Quote: ... Transient transfection was performed with the use of HilyMax liposome transfection reagent (Dojindo Laboratories) or CalPhos Mammalian Transfection Kit (Clontech). Unless otherwise noted ...
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bioRxiv - Neuroscience 2022Quote: ... Lentiviruses were purified and concentrated using the LentiX Concentrator (Clontech, PT4421-2) according to the manufacturer’s protocol ...