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Citations for Takara Bio :
9351 - 9400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... and impact of SAID1/SAID2 on SE interaction with other proteins was examined on SD-His/-Leu/-Met/-Trp quadruple dropout medium (Clontech, Cat. No. 630429) supplemented with 5 mM 3-amino-1,2,4-triazole (3-AT ...
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bioRxiv - Plant Biology 2022Quote: ... and interaction was examined on SD-Ade/-His/-Leu/-Trp quadruple dropout medium (Clontech, Cat. No. 630317 and 630323). For Y3H assays ...
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bioRxiv - Plant Biology 2022Quote: ... and ligated into pGBKT7 (Clontech, USA), where they were sequenced (Eurofins ...
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bioRxiv - Zoology 2022Quote: ... ExTaq (Takara, Shiga) for 16S ...
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bioRxiv - Physiology 2022Quote: ... The insulin-like growth factor 1 (IGF1) coding sequence was PCR amplified (CloneAmp, Takara Bio; Cat. No. 639298) from cDNA generated from a rat gastrocnemius muscle ...
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bioRxiv - Plant Biology 2022Quote: ... In-Fusion HD Cloning Kit (Takara Bio USA) was used to insert the AVR-Mgk1 fragment (Arg25-Trp85 ...
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bioRxiv - Plant Biology 2022Quote: ... and histidine (H) and containing 5-Bromo-4-Chloro-3-Indolyl α-D-galactopyranoside (X-α-gal) (Clontech) to detect interactions ...
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bioRxiv - Physiology 2022Quote: ... cDNA was generated from 200ng of RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio; Cat. No. RR047A) according to the manufacturer’s directions ...
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bioRxiv - Physiology 2022Quote: ... The rat IGF1 coding sequence was then inserted into the linearized scAAV-CMV plasmid using In-Fusion cloning (Takara Bio; Cat. No. 639650). The resulting plasmids for scAAV-CMV-GFP and scAAV-CMV-IGF1 were packaged using AAV2/9 serotype by Vector Biolabs (Malvern ...
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bioRxiv - Plant Biology 2022Quote: ... The Y2H assays were carried out in the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The pairs of corresponding recombinant constructs fused with pGBKT7 BD and pGADT7 AD were co-transformed into the Y2H Gold yeast competent cells ...
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bioRxiv - Plant Biology 2022Quote: ... annealed and inserted between EcoRI and SpeI of pHIS2.1 vector (Clontech). The GLK and GBF CDS were cloned to pGAD-T7 to generate AD fusion ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products were purified using NucleoSpin PCR clean-up kit (Takara).
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bioRxiv - Synthetic Biology 2022Quote: ... and PrimeSTAR® GXL DNA Polymerase (TaKaRa) were used for PCR reactions while DreamTaq Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Supernatant containing virus was collected 48 h later and 10-X concentrated using Lenti-X Concentrator (Takara Bio, Germany). AML-12 wild-type cells were transduced using concentrated virus and selected using 3 μg/mL of puromycin ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... PrimeScript™ RT reagent Kit with gDNA Eraser (Takara Biomedical Technology Co., Ltd. Japan) was used for reverse transcription ...
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bioRxiv - Neuroscience 2022Quote: We used the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara #634894) per the manufacturer’s instructions for reverse transcription of RNA and subsequent cDNA amplification as described (https://dx.doi.org/10.17504/protocols.io.8epv517xdl1b/v2) ...
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bioRxiv - Neuroscience 2022Quote: ... single nuclei were sorted into 8-well strip tubes containing 11.5μl of SMART-seq v4 collection buffer (Takara) supplemented with ERCC MIX1 spike-in synthetic RNAs at a final dilution of 1×10-8 (Ambion) ...
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bioRxiv - Neuroscience 2022Quote: ... and either 4 wells of 10 pg of Human Universal Reference Total RNA (Takara 636538) or 2 wells of 10 pg of Human Universal Reference and 2 wells of 10 pg Control RNA provided in the Clontech kit ...
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bioRxiv - Physiology 2022Quote: The coding sequence of PIK3CA was then cloned PIK3CA into the pcDNA3.4 vector through in-fusion cloning (Clontech) as an untagged protein or with an N-terminal polyhistidine tag ...
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bioRxiv - Physiology 2022Quote: ... PCR product were inserted into the vector backbone using In-fusion HD cloning (Takara) and transformed in Stbl3 competent cells and grown at 37°C overnight.
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bioRxiv - Neuroscience 2022Quote: ... and anti-DsRed (1:500, rabbit, Takara Bio ...
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bioRxiv - Plant Biology 2022Quote: ... the RGA5-HMA mutants and the effector genes (AVR-Pia and AVR-PikD) were separately cloned into pGADT7 (Clontech) and pGBKT7 (Clontech) ...
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bioRxiv - Plant Biology 2022Quote: ... this construct was subcloned into pBINX’ plasmid using SalI (Takara, Japan) and BamHI (Takara ...
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bioRxiv - Plant Biology 2022Quote: ... and pGBKT7 (Clontech). For the transient expression assays in N ...
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bioRxiv - Plant Biology 2022Quote: ... and BamHI (Takara, Japan). pBINX’-amjaz8 or the empty vector (EV ...
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bioRxiv - Plant Biology 2022Quote: ... The yeast strain Y2H AH109 was cotransformed with the prey and corresponding bait vectors following the protocol provided by the Yeastmaker™ yeast transformation system (Clontech). The yeast transformants were grown on SD/-Trp/-Leu medium ...
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bioRxiv - Molecular Biology 2022Quote: Isolation of miRNAs from 300 μL of each serum sample was done with NucleoSpin miRNA Plasma (Takara Bio, Inc., Tsu, Japan) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... pcDNA4TO-bio-myc-K294A was prepared from pcDNA4TO-bio-myc-mCE by using In-Fusion Site Directed Mutagenesis kit (Takara Bio). The HA-NCK1-M3 construct was a generous gift from Dr ...
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bioRxiv - Microbiology 2022Quote: ... A CVB3 genome encoding a NanoLuc luciferase (NLuc) reporter was generated using the In-Fusion HD Cloning Kit (Takara, 638909). NanoLuc luciferase was cloned from the pLenti6.2-Nanoluc-ccdB ...
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bioRxiv - Molecular Biology 2022Quote: ... and amplified by PCR using a forward primer containing a T7 promoter sequence (Supplemental Table 10) with the Advantage HF 2 kit (Takara Bio 639124) and the following cycling conditions ...
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bioRxiv - Cancer Biology 2022Quote: CPT1a knockdown cell lines were generated using the shRNA-expressing lentiviral pLKO-shRNA2 vector (No. PT4052-5; Clontech), with a puromycin selection cassette ...
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bioRxiv - Cancer Biology 2022Quote: ... The expression of mRNA was examined by real-time quantitative-PCR (qRT-PCR) using SYBR Green PCR Master Mix (Takara, Japan) and a LightCycler480 Detection System (Roche ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells or frozen tissues using the TRIzol Reagent (Takara, Japan). The complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2022Quote: ... The complementary DNA (cDNA) was synthesized using a PrimeScript reverse transcriptase reagent kit (Takara, Japan). The expression of mRNA was examined by real-time quantitative-PCR (qRT-PCR ...
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bioRxiv - Microbiology 2022Quote: ... Genotyping was performed using Terra PCR Direct Polymerase Mix (Takara) following manufacturers specifications.
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bioRxiv - Molecular Biology 2022Quote: ... and concentrated 10-fold using Lenti-X concentrator (Takara). Lentivirus aliquots were stored at -80 °C until functional titration and use ...
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bioRxiv - Neuroscience 2022Quote: ... Control RNA was either Universal Human RNA (UHR) (Takara 636538) or control RNA provided in the SMART-Seq v4 kit ...
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bioRxiv - Neuroscience 2022Quote: ... and nucleus was removed from the pipette holder and contents were expelled into a PCR tube containing lysis buffer (Takara, 634894).
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bioRxiv - Neuroscience 2022Quote: We used the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, 634894) to reverse transcribe poly(A ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-dsRed (1:500; Clontech 632496) primary antibodies in PBS/0.5% normal donkey serum/0.1% Triton-X 100 ...
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bioRxiv - Molecular Biology 2022Quote: ... The sgRNA library was prepared using a one-step PCR with ExTaq polymerase (Takara) and a mixture of P5 forward primers with staggers from 1 to 8 bp and barcoded P7 reverse primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq Kits v2 (Clontech, Takara Bio) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq Kits v2 (Clontech, Takara Bio) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 1.67X First-Strand Buffer (Takara Bio, 639538), 1.67 µM TSO (Exiqon ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.6 µL of reaction mix (16.7 U/µl SMARTScribe Reverse Transcriptase (Takara Bio, 639538), 1.67 U/µl Recombinant RNase Inhibitor (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... 1.67 U/µl Recombinant RNase Inhibitor (Takara Bio, 2313B), 1.67X First-Strand Buffer (Takara Bio ...
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bioRxiv - Neuroscience 2022Quote: The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara #634894) was used per the manufacturer’s instructions ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: ... Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviruses concentrated in Lenti-X concentrator (Takara) were used immediately to transduce HCC827 and PC9 lung cancer cell lines ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral vectors were packaged into lentivirus particles by co-transfecting Lenti-X 293T cells (Clontech) with pMDLg/pRRE (a gift from Didier Trono ...