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Citations for Takara Bio :
9251 - 9300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The mutation Pro171Leu in PETC was introduced by In-FusionR (Clontech, TAKARA, Japan) with the DNA fragment between BbvCI and SnaBI sites which was generated by PCR with If-BbvCI_P171L_gcC_F and If-SnaBI_gcC_R primers ...
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bioRxiv - Genetics 2022Quote: ... Virus titer was estimated using Lenti-X GoStix Plus (Takara, 631281) after 100x dilution ...
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bioRxiv - Cell Biology 2022Quote: ... 500 nM B/B Homodimerizer (Takara, #635058) was also added ...
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bioRxiv - Molecular Biology 2022Quote: Stable clones were grown in medium containing Tet system-approved fetal bovine serum (TaKaRa Bio), puromycin ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa Tet On 3G cells (TaKaRa Bio) were transfected with pTRE3G-BI-GFP-FLAG-p27 FL or pTRE3G-BI-GFP-FLAG-p27* ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using TaKaRa ExTaq (TaKaRa). PCR products were analyzed using a 1% agarose gel ...
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bioRxiv - Cancer Biology 2022Quote: The total RNA is extracted from the stable cell lines (C33A/HeLa) using a trizol reagent (RNA Iso Plus reagent, Takara) and from paraffin-embedded cervical cancer tumour tissue sections using the RNeasy FFPE kit (catalogue number 73504 ...
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bioRxiv - Cancer Biology 2022Quote: ... USA).and converted to cDNA using a Primescript RT reagent kit (Cat# RR037A, DSS Takara Bio India Private Ltd) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... and 20U RRI (Takara, 2313). 40μm FlowMi cell strainers were firstly wetted with PBS and filtered the resuspended nuclei into 1.5 ml Eppendorf tubes ...
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bioRxiv - Bioengineering 2022Quote: ... The solution was heated at 65°C for 5 minutes using Takara® PCR Thermal Cycler (Takara Corp., Shiga, Japan), and then gradually cooled down to 30°C over a time span of 10 minutes before settling down to 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... 5 units of recombinant DNase I (TaKaRa, Kyoto, Japan), 200 units of RevertAid™ reverse transcriptase (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The resulting mutant BAC was electroporated into NEB 10-beta cells and purified using the Nucleobond BAC 100 kit (Takara).
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bioRxiv - Cell Biology 2022Quote: ... ERManI-YFP was constructed with human ERManI cloned into peYFP-N1 (Clontech) using Hind III and Xma I yielding ERManI fused on its C-terminus to YFP through a 6 amino acid flexible linker (SGGGGS) ...
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bioRxiv - Cell Biology 2022Quote: ... Sar1[T39N]-HA in pTARGET or in IRES-GFP (Clontech) were used before 6 ...
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bioRxiv - Cell Biology 2022Quote: The GFP-AktPH construct was obtained from the laboratory of Jason Haugh (North Carolina State University, Raleigh NC)(72) and cloned into the pLVX-IRES-puro vector (Clontech). The GFP-tractin construct was a gift from Dyche Mullins (Addgene plasmid # 58473 ...
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bioRxiv - Cell Biology 2022Quote: ... and was cloned into the pLVX-IRES-puro vector (Clontech). Paxillin-pEGFP was a gift from Rick Horwitz (Addgene plasmid # 15233 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells expressing lentiviral vectors were created by following the manufacturer’s instructions for virus preparation and cell infection (Clontech). Cells were selected for expression by treatment with puromycin ...
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bioRxiv - Cell Biology 2022Quote: Low passage Lenti-X 293T cells (Clontech) were seeded onto 6-well plates coated with 0.1 mg ml−1 poly-L-lysine and transfected using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... The fragments were then assembled using In-Fusion HD cloning kit (Clontech) to obtain the desired chimeric OR ...
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bioRxiv - Neuroscience 2022Quote: ... system and the transformation was performed using Stellar competent cells (Takara Bio USA) in heat-shock ...
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bioRxiv - Microbiology 2022Quote: Human liver cDNA library was obtained from Clontech (California, USA). Dual Luciferase reporter assay kit and CellTiter96 Aqueous one solution cell proliferation assay kits were from Promega (Madison ...
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bioRxiv - Neuroscience 2022Quote: ... Ligation was done with In Fusion (Takara Bio USA) system and the transformation was performed using Stellar competent cells (Takara Bio USA ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rabbit DsRed (1:3000, Takara Bio, 632496), rabbit β3-tubulin (1:3000 ...
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bioRxiv - Neuroscience 2022Quote: ... The amplified linear PCR products containing the desired modification were ligated and transformed into Stellar™ Competent Cells (Takara Bio USA). All sequences were confirmed by DNA sequencing (UC Berkeley DNA Sequencing Facility).
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bioRxiv - Molecular Biology 2022Quote: ... Section B step 4 in the protocol was modified to account for the incorporation of UDIs (Takara SMARTer RNA Unique Dual Index Kit-96A). A total of 2 uL of each UDI was used instead of the recommended 1 uL each of the 5’ and 3’ PCR primers ...
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bioRxiv - Molecular Biology 2022Quote: Single-cell K562 libraries were prepared using the SMART-seq Stranded (SSS) Kit (Takara Bio), following the manufacturer instructions with modifications ...
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bioRxiv - Immunology 2022Quote: ... Lenti-X 293T cells (Takara) were seeded in 15-cm2 dishes at a density of 10L×L106 cells per dish and the following day were transfected with 25Lµg of spike expression plasmid with TransIT-Lenti (Mirus ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RBDs were purified using a Cobalt affinity column (HisTALON Superflow column from Takara or HiTrap TALON crude column from Cytiva ...
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bioRxiv - Biophysics 2022Quote: ... The stability of the fusion constructs was verified by gel electrophoresis and immunoblotting using an anti-GFP primary antibody (JL-8 monoclonal, Takara). Point mutations were introduced by site-directed mutagenesis (New England Biosciences) ...
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bioRxiv - Neuroscience 2022Quote: ... The yeast strains AH109 and Y187 as a partner (both from Clontech) were used.
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bioRxiv - Neuroscience 2022Quote: Jacob-LMO4 interaction was reconfirmed using fusion vectors (bait vector pGBKT7, prey vector pGADT7) using MATCHMAKER Two-Hybrid System 3 (Takara Bio Europe/Clontech, France). Co-transformed yeasts were assayed for growth on quadruple drop-out medium (SD/–Ade/–His/–Leu/–Trp ...
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bioRxiv - Neuroscience 2022Quote: Jacob-LMO4 interaction was reconfirmed using fusion vectors (bait vector pGBKT7, prey vector pGADT7) using MATCHMAKER Two-Hybrid System 3 (Takara Bio Europe/Clontech, France). Co-transformed yeasts were assayed for growth on quadruple drop-out medium (SD/–Ade/–His/–Leu/–Trp ...
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bioRxiv - Cell Biology 2022Quote: ... Aureobasidin A (TaKaRa), phytosphingosine (Tokyo Chemical Industry) ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA synthesis was performed using the M-MLV reverse transcriptase (Takara Bio, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: The In-Fusion Advantage PCR Cloning Kit (Clontech) and Gateway Cloning Technology (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... 0.1 g of leaves or fruits was mixed with 1 mL RNAiso plus according to manufacturer’s instruction (Takara, Kusatsu, Japan), then RNA was reverse-transcribed into cDNA using PrimeScript RT reagent with gDNA Eraser (Takara ...
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bioRxiv - Plant Biology 2022Quote: ... The SlBZR1 CDS was introduced into the pGBKT7 vector and then used as bait to screen the cDNA library based on the Matchmaker Gold Yeast Two-Hybrid System (Clontech). Yeast transformants were exhaustively selected on SD/-Ade/-His/-Leu/-Trp/X-α-Gal medium ...
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bioRxiv - Plant Biology 2022Quote: Y2H protein interaction assays were performed according to the manufacturer’s instructions as described in the Yeast Protocols Handbook (Clontech; TaKaRa Bio USA, Inc., Forster, CA, USA). Constructs that contained NZR3.1 ...
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bioRxiv - Plant Biology 2022Quote: ... with SYBR Green PCR master mix (TaKaRa, Dalian, China). All the genes expressed were normalized against the internal control gene MdACTIN (CN938023) ...
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bioRxiv - Plant Biology 2022Quote: ... and anti-GFP (632381; Clontech, USA) antibodies ...
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bioRxiv - Plant Biology 2022Quote: ... the entire ELF3 genomic sequence present in three HIF pairs was amplified using Ex Taq DNA Polymerase (Takara Bio, Kusatu, Shiga, Japan) and 1186 bp of promoter sequence upstream of the ELF3 start codon in HIF pair 10 was amplified using ALLin™ RPH Polymerase (highQu GmbH ...
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bioRxiv - Plant Biology 2022Quote: ... 10 μl SYBR Premix ExTaq II (Takara), 0.4 μM gene-specific primers and 20 ng cDNA ...
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bioRxiv - Microbiology 2022Quote: ... were synthesized in vitro transcription based on the corresponding cDNAs inserted into the pGEM-T easy vector with T7 RNA polymerase (Takara, Beijing, China) as previously described (22) ...
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bioRxiv - Microbiology 2022Quote: ... the lentiviral transfer plasmid pCSII-EF-luciferase (Agarwal et al., 2006), and pCMV-VSV-G (Stewart et al., 2003) using the CalPhos Mammalian Transfection Kit (Takara Bio Company, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... 25 μl PrimeSTAR GXL Premix (2x, Takara Bio, cat# R051A, Japan), 20 ng DNA and NF water to a total volume of 50 μl ...
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bioRxiv - Microbiology 2022Quote: ... cDNAs synthesized from the extracted total RNAs using a PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA, Japan) were used as templates to perform qPCR assays for the checking of the transcriptions of VEGFA ...
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bioRxiv - Microbiology 2022Quote: ... and cDNA was synthesized with a PrimeScript™ RT Reagent Kit (Takara) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Extracted RNA was treated with DNase I (Takara) to remove contaminating DNA ...
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bioRxiv - Microbiology 2022Quote: ... and used for plasmid DNA preps using the Endotoxin-Free Nucleobond Plasmid Midiprep kit (Takara Bio; 740422.10). The HIVDEP library was sequenced and contains all 4,191 sgRNAs included in the synthesis (GEO Dataset ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplification from genomic and plasmid DNA templates was performed using PrimeStar Max DNA polymerase (Takara Bio, Kusatsu, Japan) or GoTaq Master Mix (Promega ...