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Citations for Takara Bio :
9201 - 9250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 1.8 μl reaction stop solution containing 5.3% SDS and 6.6 mg/mL proteinase K (9034, Takara) was added and the sample was incubated for 60 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed with SYBR Premix Ex Taq II (RR820A, TaKaRa Bio) or TB Green Premix DimerEraser (RR091A ...
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bioRxiv - Microbiology 2022Quote: ... ORF45 and ICP0 was subcloned into the BamHI and XhoI sites of pcDNA4/TO-2xStrep (N-terminal tag) to generate pcDNA4/TO-2xStrep-ORF45 using InFusion cloning (Clontech). Deletion mutants of 2xStrep-ORF45 were generated using site-directed mutagenesis PCR with Q5 DNA Polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were treated with Dox (Clontech, 631311) at a final concentration of 1 μg/mL to induce the expression of exogenous PTBP1 and the transcription of shRNA to cleavage endogenous PTBP1 mRNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... individual pTRIPZ-PTBP1 vectors were co-transfected with psPAX2 and pMD2.G into Lenti-X 293T cells (Takara, 632180), and the supernatant was collected twice at 48 h and 72 h post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... Aer01 tagged with dTomato were labeled by incubating larvae in blocking solution containing Rabbit anti-dsRed (Takara Bio #6324960) at 1:500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized using the PrimeScript™ RT reagent Kit (TAKARA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... qPCR reactions were performed with iQ SYBR green supermixture kit (TAKARA) in the CFX96TM Real-Time PCR System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5% SDS) and 50 μl of proteinase K (Takara, 9034) were added ...
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bioRxiv - Molecular Biology 2022Quote: ... were transcribed using in vitro Transcription T7 Kit (Takara). Template DNA for sna probe was PCR amplified from genomic DNA using primers (5 ’-CGT AAT ACG ACT CAC TAT AGG GCA GTT GGC TTA ACA GTA CTG-3 ’ ...
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Development of immortalized rhesus macaque kidney cells supporting infection with a panel of virusesbioRxiv - Microbiology 2022Quote: ... This vector has a shortened C-stretch at the 5’ end of the internal ribosomal entry site (IRES) and the modified multiple cloning site of pQCXIP-mcs (20) which was ultimately derived from pQCXIP (Clontech, Palo Alto, CA, USA). Finally ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant adenovirus was produced using an Adenovirus Dual Expression Kit (Takara Bio) in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The purified virus titer was determined using an Adeno-X Rapid Titer kit (Takara Bio). Sex and age at sampling for the cells were as follows ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant adenovirus was prepared in HEK293 cells and purified with an Adeno-X Virus Purification kit (Takara Bio). The purified virus titer was determined using an Adeno-X Rapid Titer kit (Takara Bio) ...
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bioRxiv - Microbiology 2022Quote: ... mRNA amounts were quantified with Premix Ex Taq (Perfect Real Time) (Takara Bio), and the Applied Biosystems 7300 Real-Time PCR system (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized from total RNA with a PrimeScript RT Reagent Kit (Takara Bio). mRNA amounts were quantified with Premix Ex Taq (Perfect Real Time ...
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bioRxiv - Microbiology 2022Quote: ... RNA was then purified and resuspended with SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions for mRNA amplification using 5’ template switching polymerase chain reaction (PCR) ...
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bioRxiv - Molecular Biology 2022Quote: 50 ng “treated” RNA sample and 10 ng “untreated” RNA sample were subjected to library construction using SMARTer® Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara Bio, 634485) according to the manufacturer’s protocol with several modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were split in two and passed through two CHROMA SPIN+TE-100 columns (Takara 636072), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: We amplified Complementary DNAs (cDNAs) of genes by PCR using PrimeSTAR Max DNA polymerase (TAKARA Bio), and cloned the PCR products into overexpression vectors EGFP-N1 (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... Pico-Input v2 (Takara). Libraries were sequenced on a NovaSeq 6000 (Illumina) ...
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bioRxiv - Molecular Biology 2022Quote: The pCMV DsRed-Express2 (Clontech 632539) expression plasmid was stably transfected into wildtype T-REx-293 cells by same protocol described above and was selected using Neomycin at 500 µg/mL for seven days ...
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bioRxiv - Neuroscience 2022Quote: Library generation was performed as previously described27 using the Clontech SMART-Seq HT (Takara) kit ...
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bioRxiv - Neuroscience 2022Quote: ... and a subset (approximately 75,000 cells) was pelleted prior to RNA extraction using the NucleoSpin RNA Plus XS kit (Takara) following manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: ... both non-replicant RGS14414- and vehicle-lentivirus (empty vector) were prepared and titered using the Lenti-XTM (Clontech, France) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Briefly, the RGS14414 gene (GenBank, AY987041) was cloned into the commercial vector p-LVX DsRed Monomer-C1 (Clontech, France) using DNA recombinant technology ...
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bioRxiv - Molecular Biology 2022Quote: ... In-Fusion HD Cloning Kit (Clontech 639649), and XL10-Gold Ultracompetent E ...
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bioRxiv - Neuroscience 2022Quote: ... dsRED (rabbit polyclonal, Living Colors, Clontech, Cat no. 632496); MAP2 (mouse monoclonal ...
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bioRxiv - Molecular Biology 2022Quote: ... reverse transcriptions were performed using PrimeScriptTM RT reagent kit (TaKaRa, Ohtsu, Japan). RT-qPCR was performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted using RNAiso Plus (Takara, Ohtsu, Japan). As described previously (Yu et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Detection relied on a 32P-labeled DNA probe specific for GFP sequence synthesized with a random priming labeling kit (Takara) followed by exposure to film and development as described (43).
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bioRxiv - Molecular Biology 2022Quote: ... pSiCBL4::LacZ or pSiCBL7::LacZ were co-transformed into yeast strain EGY48 according to the Yeast Protocols Handbook (Clontech). Transformed yeast (Saccharomyces cerevisiae ...
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bioRxiv - Molecular Biology 2022Quote: The respective sequences of the proteins were cloned into pGBKT7 and pGADT7 vectors from Clontech which harbor either the DNA binding domain or activation domain at the N-termini ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified and library was generated using Thruplex DNA-seq Kit from Takara according to manufactures’ instructions ...
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bioRxiv - Cell Biology 2022Quote: Mouse NIH/3T3 (ATCC, cat# CRL-1658), human IMR-90 (ATCC, cat# CCL-186) and Lenti-X™ 293T (Takara Bio, cat# 632180) cell lines were cultured in full-DMEM (Corning ...
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bioRxiv - Cell Biology 2022Quote: ... PRR14-GFP 1-212 PCR products were sub-cloned into pLVX-TetOne-Puro (Takara Bio, cat# 631847) vector using T7 ligase (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... and mixture with Lenti-X concentrator (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and cloned into pRetro-X-tight-PUR (Clontech), using NotI and EcoRI sites ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were assembled into pEMC vector one-by-one using in-fusion HD cloning kit (Takara), generating pEMC-eGFP-MBaMV ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was reverse-transcribed using a PrimeScript RT reagent Kit with gDNA Eraser (Cat: RR047A, TAKARA, Shiga, Japan), and qPCR was performed on a Bio-Rad CFX96 machine (Hercules ...
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bioRxiv - Molecular Biology 2022Quote: ... and subcloned into pTRE3G-BI (TaKaRa Bio) to construct pTRE3G-BI-GFP ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was prepared using Primescript Reverse Transcriptase (TaKaRa Bio) and random primers ...
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bioRxiv - Microbiology 2022Quote: ... we conducted additional long PCR using LA Taq polymerase (TaKaRa Bio Inc.) to detect occupied attB and confirm the functionality of primers used for detection of empty attB ...
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bioRxiv - Microbiology 2022Quote: ... and attS was performed using TaKaRa Ex Taq (TaKaRa Bio Inc., Kusatsu, Japan), and genomic DNA of 04Ya249 ...
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bioRxiv - Molecular Biology 2022Quote: ... and G418 were purchased from TaKaRa Bio (Otsu ...
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bioRxiv - Cancer Biology 2022Quote: ... The converted cDNA was amplified using gene-specific primers and TB Green® Premix Ex Taq™ II (Cat# RR820A; DSS Takara Bio India Private Ltd) to quantify the expression of the genes using a real-time PCR system (Applied Biosystem Quant Studio 7 Flex ...
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bioRxiv - Immunology 2022Quote: ... Ten ng total RNA was used as template for SMARTer® Stranded Total RNA-Seq Kit – Pico Input Mammalian (Takara Bio.) following the manufactureŕs recommendation.
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bioRxiv - Immunology 2022Quote: ... All cloning reactions were performed using In-Fusion HD cloning kit (Clontech) in a standard reaction mixture.
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bioRxiv - Neuroscience 2022Quote: ... 3 × 106 HEK293T cells (Clontech) were seeded on a 10 cm cell culture dish and transfected after 24 h with GenJet transfection reagent (SignaGen) ...
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bioRxiv - Plant Biology 2022Quote: ... The mutation Pro171Leu in PETC was introduced by In-FusionR (Clontech, TAKARA, Japan) with the DNA fragment between BbvCI and SnaBI sites which was generated by PCR with If-BbvCI_P171L_gcC_F and If-SnaBI_gcC_R primers ...