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Citations for Takara Bio :
9101 - 9150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: cDNA fragments corresponding to Inka1 and Inka2 ORFs were isolated using RT-PCR of the RNAs of E12 embryonic and adult mice brains and subcloned in-frame into a pEGFP-C2 expression vector (Takara Bio Inc., Shiga, Japan). To construct the CAG promoter-driven Inka2 in mammalian cells ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: mouse anti-Nc82 (Laissue et al., 1999) rabbit anti-DsRed (TaKaRa Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... Each sample was then incubated at 37°C for 30 min with 20 U of mRNA-Interferase™ MazF enzyme (Takara, Cat# 2415A) in a 10 μL volume reaction containing 1X MazF reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... and COUP-TF2LBDΔAF2-TRF1 were generated using the InFusion cloning method (Clontech). Vectors were digested with BamHI and incubated overnight at 37 °C (5 µg Vector ...
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bioRxiv - Molecular Biology 2022Quote: ... One microgram of total RNA was used as a template in reverse transcription reactions using 0.2 mM dNTP (TakaRa Bio), 1 U/μL ribonuclease inhibitor (porcine liver ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 U/μL ribonuclease inhibitor (porcine liver) (TaKaRa Bio), 0.25 U/μL AMV reverse transcriptase XL (TaKaRa Bio) ...
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bioRxiv - Neuroscience 2022Quote: ... and were replaced with the FP fragment in Cyan pre-eGRASP(p32) and post-eGRASP via Infusion (Takara Bio, Shiga, Japan) to yield the Pre-Sphere-iGluSnFR(p32 ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed using the TB Green Ex Taq II Mix (Takara Bio) and the Thermal Cycler Dice Real-Time System (Takara Bio).
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bioRxiv - Neuroscience 2022Quote: ... Real-time PCR was performed using TB Green Premix Ex Taq II (Takara, Japan) and ViiA7 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA band was cut out and purified using the Nucleospin® Extract II Kit (Clontech Laboratories) protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products covering the whole sesRNA region was generated with CloneAmp HiFi PCR Premix (Takara, Cat. No. 639298), and purified with NucleoSpin Gel and PCR Clean-up kit (Takara ...
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bioRxiv - Molecular Biology 2022Quote: ... The 2× SYBR Green qPCR Mix Kit (TaKaRa, Japan) and the cDNA concentration and primers described above were used ...
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bioRxiv - Molecular Biology 2022Quote: ... and also remaining polymerase enzyme by use of Nucleospin® Extract II Kit (Clontech Laboratories) following Manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The titer of adenovirus was examined using an Adeno-XTM Rapid Titer Kit (Takara Bio USA) and determined to be 3.4 × 1011 ifu/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... The edit locus was PCR amplified using Titanium® Taq DNA Polymerase (Takara, 639208) followed by Sanger Sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... The titer of harvested lentivirus was established using the Lenti-X RT-qPCR Titration Kit (Takara Bio, Cat. no. 631235). A representative calibration plot and LV tittering of pAIO is added as Supplementary data S4.
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bioRxiv - Molecular Biology 2022Quote: ... 6xHN Polyclonal Antibody (1:2000, Takara, CA, USA) at 4°C for overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... red fluorescence protein (DsRed) and NK-NT or NKN1 fragments were cloned into pET6xHN-N Vector (Takara, CA, USA). HEK293T cells were cultured in FP medium (DMEM containing 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplicons were cloned into vector pRACE (Takara) and individual clones were selected for nucleotide sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... The amplified PCR products and pJM101/L1.3FLAG plasmid DNA then were digested with NotI and AgeI and ligated using the DNA Ligation Kit Mighty Mix (TaKaRa Bio, Shiga, Japan) at 16°C for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and 200 U SMARTScribe Reverse Transcriptase (Takara). EBIV M/L segment-specific sequences were amplified by PCR including Universal Primer A Mix (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: ... one subjected to RT-PCR using PrimeScript™ One Step RT-PCR Kit Ver.2 (Dye Plus) (Takara, Japan), then the region between S-5’UTR and N protein-ORF genes was amplified to monitor the expression of eGFP.
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bioRxiv - Molecular Biology 2022Quote: The G3BP1 cDNA sequence was amplified from a HeLa-JVM total cDNA library and concurrently inserted in-frame with a mCherry-coding sequence into a lentiviral vector (pCW)110 using the in-Fusion Cloning Kit (TaKaRa Bio). The HERC5 ...
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bioRxiv - Molecular Biology 2022Quote: ... and Tet System approved FBS (631106, Takara) containing medium was used to culture doxycycline (Dox ...
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bioRxiv - Molecular Biology 2022Quote: Synthesis of cDNA with biotinylated 3’ adaptor ligation was performed using a Small RNA Cloning Kit (Takara Bio Inc., Kusatsu, Japan) as previously reported (20 ...
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bioRxiv - Molecular Biology 2022Quote: ... RT–PCR and agarose gel electrophoresis were performed after RNA extraction with TRIzol (Takara, China). The primers are listed in the Supplementary Table 4.
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative RT-PCR was used to determine gene expression using the SYBR Green Realtime Master Mix (Takara, DaLian, China). Table 1 contains a list of all primers used in this study ...
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bioRxiv - Molecular Biology 2022Quote: ... and reverse transcribed using the PrimeScript™ RT reagent Kit (Takara). Quantitative RT-PCR was used to determine gene expression using the SYBR Green Realtime Master Mix (Takara ...
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bioRxiv - Molecular Biology 2022Quote: ... The bait stain was combined with a Mate & Plate™ Human Heart library (Takara Bio) for 24 hours after which cells were plated on selective synthetic defined (SD ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatant was incubated with equilibrated TALON Metal Affinity Resin (Takara Bio USA, Inc., CA) for 20 min on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... the repeat insert was further subcloned to a doxycycline-inducible (Tet-On, Clontech) expression vector containing 12x MS2 hairpins.
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bioRxiv - Molecular Biology 2022Quote: ... The cDNAs (1-5 ng RNA equivalents) were used for real-time PCR amplification using SYBR Premix Ex Taq II (Takara/Clontech) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... The cDNAs (1-5 ng RNA equivalents) were used for real-time PCR amplification using SYBR Premix Ex Taq II (Takara/Clontech) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from jejunum tissues using RNA isolation reagent (TaKaRa Biotechnology, Dalian, Liaoning, China) according to the manufacturer’s instructions for animal tissue ...
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bioRxiv - Molecular Biology 2022Quote: ... Reaction mix contained 7.5 µL SYBR TB Green® Premix Ex Taq™ (RR420A, Takara) with 0.5 µM forward and reverse primers and 2.5 µL of each cDNA sample ...
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bioRxiv - Plant Biology 2022Quote: ... The downstream genomic region was then amplified using the primer set MpHEC1_CDS_IF_F and MpHEC1_3UTR_IF_R2 and integrated into the first binary plasmid with an In-Fusion HD Cloning Kit (Clontech) using the BamHI and AscI sites ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized using the PrimeScript Reverse Transcriptase kit (Takara, Japan) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... DNA was purified using NucleoSpin Gel and PCR Clean-up kits (Takara, 740609.250). Libraries were prepared identically to SHARE-seq ATAC libraries.
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bioRxiv - Neuroscience 2022Quote: ... 0.2U/ml Recombinant ribonuclease inhibitor (Takara, #2313B). A second centrifugation (same conditions ...
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bioRxiv - Plant Biology 2022Quote: ... and pDONR221-f1/pSWEET14:SWEET9 by In-Fusion® (TaKaRa, Shiga, Japan) after the linearization at the XbaI restriction site ...
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bioRxiv - Plant Biology 2022Quote: ... One base of 5’ dA overhang was added to the PCR amplicon of pMYB93 using Taq polymerase (Takara Bio Inc. Shiga, Japan), which was then cloned into pENTR5’-TOPO (Thermo Fischer Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... qPCRs were performed using SYBR Premix Ex Taq II reagent kit (Takara, Japan) and CFX96 Touch Real-Time PCR Detection System (Biorad ...
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bioRxiv - Plant Biology 2022Quote: ... RNA was isolated from 500 mg pulverized tissue using RNAiso Plus reagent (Takara, Japan), as recommended by the manufacturer ...
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bioRxiv - Plant Biology 2022Quote: ... β-galactosidase activity in liquid cultures was assayed using CPRG as substrate as described in the Yeast Protocols Handbook (Takara).
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bioRxiv - Plant Biology 2022Quote: ... pastoris was transformed as described in the yeast protocol handbook (Clontech, Mountain View, USA). Gene replacement and transformation assays for U ...
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bioRxiv - Plant Biology 2022Quote: ... bait and linker proteins were cloned into the appropriate position of the pBridge vector (Clontech, Mountain View, California), which encodes a GAL4 DNA binding domain and a linker protein ...
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bioRxiv - Plant Biology 2022Quote: ... All matings were performed as detailed in the Yeast Protocols Handbook (Clontech, Mountain View, California) using the 96-well plate format ...
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bioRxiv - Plant Biology 2022Quote: ... the gene of interest was cloned from its pENTR-STOP template using primers pDAN2441/pDAN2442 (Table S2) and recombined into the first MCS of pBridge digested with EcoRI using In-Fusion HD cloning (Clontech, Mountain View, California) or using primers pDAN2443/pDAN2444 (Table S2 ...
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bioRxiv - Plant Biology 2022Quote: ... For cloning into the pBridge vector (Clontech, Mountain View, California), the gene of interest was cloned from its pENTR-STOP template using primers pDAN2441/pDAN2442 (Table S2 ...
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bioRxiv - Plant Biology 2022Quote: ... bait proteins were cloned into the pGBKT7 vector which encodes the GAL4 DNA binding domain and then transformed into the Y2H Gold strain (Clontech, Mountain View, California) and plated on SD/- Trp to select for positive transformants ...