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Citations for Evrogen :
301 - 350 of 381 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Input and bound fractions were subjected to SDS-PAGE followed by immunoblotting analysis with anti-TagRFP antibody (Evrogen, Moskau, Russia) and anti-GFP antibody (3H9 ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit anti-Tag(CGY)FP (Evrogen) was used at 1:1000 to detect mTagGFP2 ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-tRFP (1:1000 Evrogen EVN-AB233-C100).
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bioRxiv - Cell Biology 2020Quote: ... we PCR amplified the coding region of the far-red fluores-cent protein turboFP635 (Evrogen) using primer sequences 5’-TGTACCGGTCTCGAGGCCACCAT-GGTGGGTGAGG and 5’-AGATCCGGAGCTGTG-CCCCAGTTTGCTA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For polymer chain reactions we used Evrogen PCR kits with Hot Start thermostable polymerase (HS Taq polymerase, Evrogen, Russia). PCR reactions were carried out for each locus separately in a total volume of 14.1 μl (0.2 μl of a primer mixture containing forward and reverse primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the number of molecules in each library was validated by real-time PCR using 2.5x Reaction mixture for PCR-RV in the presence of EVA Green (SINTOL, Russia) and primers for Illumina adapters (Evrogen, Russia). Further ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA molecules corresponding to the rRNA transcripts were depleted using the duplex-specific nuclease (Evrogen), as described elsewhere (42) ...
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bioRxiv - Molecular Biology 2020Quote: pVimentin-Padron2: The coding sequence of Padron2 was inserted into pmKate2-vimentin (Evrogen, Moscow, Russia) with restriction enzymes AgeI and NotI ...
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bioRxiv - Molecular Biology 2020Quote: ... Cytosolic expression was facilitated by expression of free Padron2 inserted into the TagRFP-N vector (Evrogen, Moscow, Russia) with enzymes AgeI and NotI ...
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bioRxiv - Molecular Biology 2020Quote: ... pKeratin-Padron2: The RSFP coding sequence of Padron2 was inserted into pTagRFP-keratin (Evrogen, Moscow, Russia) with restriction enzymes KpnI and NotI ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA library was then normalized using the Trimmer-2 cDNA normalization kit (Evrogen, Moscow, Russia).
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bioRxiv - Molecular Biology 2020Quote: ... PCR product was cloned to t-vector (pAL2-T, Evrogen, Russia) and sequenced by Sanger for at least 10 clones for each point.
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription was performed with random hexamer primers using the MMLV RT kit (Evrogen, Russia). The obtained cDNA was used as a template in RT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-PCR was performed with Taq polymerase (Evrogen, Russia) or Phusion High-Fidelity DNA polymerase (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... pre-washed cells were lysed in x1 Laemmli buffer and analyzed with one-dimensional PAGE followed by western blotting using Anti-TurboGFP(d) antibodies (Evrogen, Russia).
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bioRxiv - Molecular Biology 2020Quote: ... we used rabbit polyclonal Anti-tRFP and Anti-TurboGFP(d) antibodies (Evrogen, Russia) diluted at 1:7000 ...
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bioRxiv - Developmental Biology 2020Quote: ... and anti-tRFP Rabbit (Evrogen, 1:250). Secondary antibodies used were goat anti-Rabbit Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA fragments encoding M9M and Bimax2 peptides were inserted into the pTagRFP-C vector (Evrogen).
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bioRxiv - Cell Biology 2020Quote: Cells were maintained in anti-bleaching live cell visualization medium (DMEMgfp; Evrogen), supplemented with 10% fetal bovine serum at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... Tag-blue fluorescent protein (Tag-BFP, Evrogen), Nlgn3Δ ...
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bioRxiv - Neuroscience 2020Quote: ... pTagBFP-actin vector was from Evrogen (#FP174) 59 ...
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bioRxiv - Systems Biology 2020Quote: Cells were plated on 25 mm diameter coverslips (0.17 mm thick) in non-fluorescent media (DMEM gfp-2 with rutin; Evrogen). Coverslips were mounted in a temperature-controlled chamber with CO2 and imaged on an inverted OMXv3 Deltavision microscope in time-lapse mode ...
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bioRxiv - Cell Biology 2020Quote: ... pHyPer-cyto vector (FP942, Evrogen, Moscow, Russia) (Belousov et al. ...
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bioRxiv - Bioengineering 2020Quote: ... was synthesized by Evrogen and cloned into the pet302-NT-His plasmid ...
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bioRxiv - Biochemistry 2020Quote: ... All primers were synthesized by Evrogen (Russia); their sequences are listed in Supplemental Table S1 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-tRFP (tagBFP, AB233, Evrogen, Moscow, Russia) and mouse anti-GAPDH (MAB374 ...
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bioRxiv - Plant Biology 2020Quote: ... an anti-TagRFP antibody (Evrogen) was added to the beads ...
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bioRxiv - Biochemistry 2020Quote: ... First strand of cDNA was synthesized using Mint kit (Evrogen) with primers for k-chain (TTG TCG TTC ACT GCC ATC AAT C) ...
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bioRxiv - Biochemistry 2020Quote: ... Obtained DNA was purified and cloned into pKAN-T (Evrogen) vector that was subsequently sequenced ...
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bioRxiv - Biochemistry 2020Quote: HT1080 cells were plated in wells of Lab-Tek II chamber and co-transfected with pTwist-EF1a-nCoV-2019-S-2xStrep and pTagGFP2-C (Evrogen) plasmids ...
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bioRxiv - Plant Biology 2020Quote: Amplification of TALE genes was performed via a two-step PCR process in conjunction with primers 5’-GATCCCATTCGTTCGCGCACACCAAGTC-3’ and 5’-CTCCATCAACCATGCGAGCTCCTCTTCG-3’ and Taq DNA polymerase (Evrogen, Russia) under the following conditions ...
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bioRxiv - Plant Biology 2020Quote: ... referred to as yfp (Evrogen, Moscow, Russia) (Shagin et al. ...
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bioRxiv - Microbiology 2020Quote: ... The quantitative RT-PCR experiments were performed in triplicates on two independently grown colonies for each isolate in the sample with the use of OneTube RT-PCR SYBR Kit (Evrogen, Russia). The reactions were carried out on CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-tRFP (Evrogen)) ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted from each fraction using the ExtractRNA kit (Evrogen, Russia). In each fraction ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was extracted using the ExtractRNA kit (Evrogen, Russia), the quality and quantity of preparations of total RNA and RNA from polysomal and monosomal fractions of plants was evaluated on an Agilent Bioanalyzer 2100 ...
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bioRxiv - Plant Biology 2020Quote: ... For cDNA synthesis MINT (Evrogen, Cat. No SK001, Moscow, Russia) was used ...
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bioRxiv - Neuroscience 2020Quote: ... human four repeat domain (4R) Tau (amino acids 243 to 375) with mutations P301L and V337M was fused to GFP or FR (Evrogen) with an 18-amino acid flexible linker (EFCSRRYRGPGIHRSPTA) ...
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bioRxiv - Microbiology 2020Quote: ... Chlamydiae were stained with polyclonal a rabbit anti-tRFP antibody (Evrogen, Cat. # 233), which recognized the RFP mKate protein ...
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bioRxiv - Plant Biology 2020Quote: ... thermocycler using the ScreenMix-HS kit (Evrogen, Moscow, Russia). The PCR cycling conditions were as follows ...
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bioRxiv - Microbiology 2020Quote: ... This was incubated with the appropriate antibodies (anti-GFP polyclonal antibody and anti-tRFP antibody, Evrogen, reference # AB233) and revealed with Roche LumiLight ECL kit after incubation with secondary antibody.
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bioRxiv - Molecular Biology 2020Quote: ... pET-NS1 was purified using a Plasmid Miniprep Kit (Evrogen, BC021). Commercial plasmid sequencing was performed using T7 primers at Evrogen (Moscow ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA bands were excised with subsequent purification of PCR products using a Cleanup Standard Kit (Evrogen, BC022). Restriction enzymes ...
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bioRxiv - Microbiology 2020Quote: The plasmid expressing phoP and phoQ was constructed by cloning the PCR fragment amplified with the Tersus DNA polymerase (Evrogen) and the phoPQf1 and phoPQr primers into the low copy number vector pZH449.
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bioRxiv - Microbiology 2020Quote: ... The fixed cells were harvested (8000 g, 5 min, 4 °C) and resuspended in 1 mL of ExtractRNA Reagent (Evrogen, Russia) and the subsequent procedures were performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-mKate (1:2500, Evrogen #AB233), anti-Cas9 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained in anti-bleaching live cell visualization medium (DMEMgfp; Evrogen), supplemented with 10% fetal bovine serum at 37°C in 5% CO2 and rutin at a final concentration of 20 mg/l.
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bioRxiv - Neuroscience 2020Quote: ... (ii) the same solution containing the primary antibody overnight at 4°C (anti-tRFP, 1:1000, Evrogen; anti-GABA ...
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bioRxiv - Neuroscience 2020Quote: ... 1.2 μg of KillerRed-dMito (Evrogen, Moscow, Russia) or mCherry-Mito-7 (Addgene #55102 ...
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bioRxiv - Genomics 2020Quote: ... USA) and normalized using the Trimmer cDNA Normalization kit from Evrogen (Moscow, Russia). The libraries were sequenced on a 454 genome sequencer FLX Titanium machine (Roche ...